RNA is a long macromolecule consisting of nucleotides joined by phosphodiester bridges. The main types of RNA are messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). mRNA carries the genetic code from DNA to the ribosome for protein synthesis. tRNA transfers amino acids to the ribosome during protein synthesis according to the mRNA sequence. rRNA is the major component of ribosomes and plays a catalytic and structural role in protein synthesis.
RNA splicing, in molecular biology, is a form of RNA processing in which a newly made precursor messenger RNA transcript is transformed into a mature messenger RNA. During splicing, introns are removed and exons are joined together.
Transcription and post-transcriptional modification.Abhishek Dahal
A slide about Transcription and Post-transcription modification prepared for undergraduates understanding but PG levels may find it good for revision and handy for exams.
For MBBS, BDS and General Biochemistry students, coding strand, sense strand, anti-sense strand, promoter, enhancers, silencers, TATA box, Goldberg Hogness box, alternative spilicing, post-transcriptional modification
Ribose Nucleic Acid, also named RNA, is the intermediary of DNA and protein. Compared with DNA, RNA has less molecular-weight but more classification.With the development of all kinds of research, scientists found that RNA plays an important role in the evolution of life. I hope this article can share basic knowledge with you and can make you have a better understanding on RNA.
RNA splicing, in molecular biology, is a form of RNA processing in which a newly made precursor messenger RNA transcript is transformed into a mature messenger RNA. During splicing, introns are removed and exons are joined together.
Transcription and post-transcriptional modification.Abhishek Dahal
A slide about Transcription and Post-transcription modification prepared for undergraduates understanding but PG levels may find it good for revision and handy for exams.
For MBBS, BDS and General Biochemistry students, coding strand, sense strand, anti-sense strand, promoter, enhancers, silencers, TATA box, Goldberg Hogness box, alternative spilicing, post-transcriptional modification
Ribose Nucleic Acid, also named RNA, is the intermediary of DNA and protein. Compared with DNA, RNA has less molecular-weight but more classification.With the development of all kinds of research, scientists found that RNA plays an important role in the evolution of life. I hope this article can share basic knowledge with you and can make you have a better understanding on RNA.
RNA is a ribonucleic acid that helps in the synthesis of proteins in our body. This nucleic acid is responsible for the production of new cells in the human body. It is usually obtained from the DNA molecule.
Ribonucleic acid (RNA) is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes. RNA and DNA are nucleic acids, and, along with proteins and carbohydrates, constitute the four major macromolecules essential for all known forms of life. Like DNA, RNA is assembled as a chain of nucleotides, but unlike DNA it is more often found in nature as a single-strand folded onto itself, rather than a paired double-strand.
Unlocking Productivity: Leveraging the Potential of Copilot in Microsoft 365, a presentation by Christoforos Vlachos, Senior Solutions Manager – Modern Workplace, Uni Systems
Sudheer Mechineni, Head of Application Frameworks, Standard Chartered Bank
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zkStudyClub - Reef: Fast Succinct Non-Interactive Zero-Knowledge Regex ProofsAlex Pruden
This paper presents Reef, a system for generating publicly verifiable succinct non-interactive zero-knowledge proofs that a committed document matches or does not match a regular expression. We describe applications such as proving the strength of passwords, the provenance of email despite redactions, the validity of oblivious DNS queries, and the existence of mutations in DNA. Reef supports the Perl Compatible Regular Expression syntax, including wildcards, alternation, ranges, capture groups, Kleene star, negations, and lookarounds. Reef introduces a new type of automata, Skipping Alternating Finite Automata (SAFA), that skips irrelevant parts of a document when producing proofs without undermining soundness, and instantiates SAFA with a lookup argument. Our experimental evaluation confirms that Reef can generate proofs for documents with 32M characters; the proofs are small and cheap to verify (under a second).
Paper: https://eprint.iacr.org/2023/1886
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GraphSummit Singapore | The Art of the Possible with Graph - Q2 2024Neo4j
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UiPath Test Automation using UiPath Test Suite series, part 6DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 6. In this session, we will cover Test Automation with generative AI and Open AI.
UiPath Test Automation with generative AI and Open AI webinar offers an in-depth exploration of leveraging cutting-edge technologies for test automation within the UiPath platform. Attendees will delve into the integration of generative AI, a test automation solution, with Open AI advanced natural language processing capabilities.
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What will you get from this session?
1. Insights into integrating generative AI.
2. Understanding how this integration enhances test automation within the UiPath platform
3. Practical demonstrations
4. Exploration of real-world use cases illustrating the benefits of AI-driven test automation for UiPath
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What is generative AI
Test Automation with generative AI and Open AI.
UiPath integration with generative AI
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Alt. GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using ...James Anderson
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The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
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One such alternative that has garnered significant attention and acclaim is Nitrux Linux 3.5.0, a sleek, powerful, and user-friendly Linux distribution that promises to redefine the way we interact with our devices. With its focus on performance, security, and customization, Nitrux Linux presents a compelling case for those seeking to break free from the constraints of proprietary software and embrace the freedom and flexibility of open-source computing.
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CI/CD with in UiPath
End-to-end overview of CI/CD pipeline with Azure devops
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Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Epistemic Interaction - tuning interfaces to provide information for AI support
Rna
1. RNA
RNA is a long un-branched macromolecule consisting of nucleotides joined by
Phosphodiester Bridge. The sugar unit is ribose. The bases are Adenine, Uracil, and
Guanine & Cytosine.
RNA molecules are usually single stranded, except some viruses. In fact the
proportion of adenine differs from that of uracil and the proportion of guanine
differs from that of cytosine, in most RNA molecules.
RNA molecules have region of double helical structure that are produced by the
formation of hairpin loops. In these region A pairs with U and G pairs with C.
Cell contains several kinds of RNA:
1. Messenger RNA(mRNA):
i) It is the template for protein synthesis. Also called the information carrier
in protein synthesis.
ii) The base composition of the messenger should reflect the base
composition of the DNA that specifies it.
iii) An mRNA is produced from each genes or group of genes that is to be
expressed.
iv) mRNA is a very heterogeneous class of molecule. They correctly assumed
that three nucleotides code for one amino acid.
v) The messenger should be synthesized and degraded very rapidly.
vi) In E.coli, the average length of an mRNA is about 1.2kb.
2. 2. Transfer RNA(tRNA):
i) Carries amino acids in the activated form to the ribosome for peptide bond
formation, in such a sequence that can be determined by the mRNA
template.
ii) There is at least one kind of tRNA for each of the 20 amino acids.
iii) Transfer RNA consists about 75 (approximately 73-93) nucleotides, which
makes it smallest of the RNA molecule.
3. Ribosomal RNA(rRNA):
i) It is the major component of ribosome.
ii) It plays both a catalytic and structural role in protein synthesis.
iii) rRNA is the most abundant of the three types of RNA. tRNA comes next,
and then mRNA constitutes only 5% of the total RNA.
4. Small nuclear RNA(snRNA):
i) Eukaryotic cells contain additional small RNA molecule that participate in
the splicing of RNA exons.
RNA polymerase I, II, III
RNA polymerase I (pol I): that only transcribes rRNA.
RNA polymerase II (pol II): catalyzes the transcription of DNA to synthesize precursor
of mRNA and most snRNA and microRNA (22 nucleotides). A wide range og
transcription factors are required for it to bind to its promoters & begin
transcription.
RNA polymerase III (pol III): transcribes DNA to synthesize tRNA and other small
RNA.
Transcription begins near promoter sites and ends at a terminator sites.-Explain.
Promotor: A promoter is a region of DNA that facilitates the transcription of a
particular gene. RNA polymerase binds in the promoter site of DNA.
DNA templates contain regions called promoter sites that specifically bind RNA
polymerase and determine where transcription begins.
3. Eukaryotic genes encoding proteins have promoter sites with a TATAAAA sequence
centered at about -25 (25 nucleotides on the 5’ side of the first one transcribed). This
is called TATA box. Many eukaryotic promoters also have CAAT box with a
GGNCAATCT consensus sequence centered at about -75.
Transcription of eukaryotic gene is further stimulated by enhancer sequences which
can be quite distant (up to several kilo bases) from the start site.
Termination: RNA polymerase proceeds along the DNA template and transcribes one
of its strands until a terminator sequence is reached. That sequence codes for a
termination signal, a stable hairpin like structure is formed. This hairpin is formed by
base pairing of self complementary sequence that is rich in G & C. The termination is
also done by rho protein.
The features of tRNA:
All known tRNA has the following features:
1. Each is a single chain containing between 73 and 93 ribonucleotides.
2. They contain many unusual bases, typically between 7 and 15 per molecule. Some
are methylated or dimethylated derivatives of A, U, C, and G formed by enzymatic
modification of a precursor tRNA. Methylation prevents the formation of certain
base pairs, thereby rendering some of the bases accessible for other interactions. In
addition, methylation imparts a hydrophobic character to some regions of tRNAs,
4. which may be important for their interaction with synthetases and ribosomal
proteins.
3. About half the nucleotides in tRNAs are base-paired to form double helices. Five
groups of bases are not base paired in this way:
The 3’ CCA terminal region, which is part of a region called the acceptor stem;
The TψC loop, which acquired its name from the sequence ribothyminepseudouracil-cytosine;
The "extra arm," which contains a variable number of residues;
The DHU loop, which contains several dihydrouracil residues;
The anticodon loop.
The structural diversity generated by this combination of helices and loops
containing modified bases ensures that the tRNAs can be uniquely distinguished,
though structurally similar overall.
4. The 5’ end of a tRNA is phosphorylated. The 5’ terminal residue is usually pG.
5. 5. The activated amino acid is attached to a hydroxyl group of the adenosine residue
located at the end of the 3’ CCA component of the acceptor stem.
6. The anticodon is present in a loop near the center of the sequence. This loop
contains seven bases.
Restriction Enzyme
Restriction enzyme splits DNA into specific fragments. It is also called Restriction
endonuclease. They recognize specific base sequence in double helical DNA and
cleave both strands of the duplex at specific places.
They are used for:
1.
2.
3.
4.
For example:
Analyzing chromosome structure.
Sequencing very long DNA molecule.
Isolating genes &
Creating new DNA molecule that can be cloned.