This document provides an overview of sequence analysis, including defining sequence analysis, its applications, DNA sequencing methods, protein sequencing, sequence assembly, sequence alignment, and biological databases. Sequence analysis involves subjecting a DNA, RNA, or protein sequence to analytical methods to understand its features, function, structure, or evolution. It includes sequencing, sequence assembly, alignment, and searching databases. The main applications are comparing sequences to find similarity and relatedness, identifying intrinsic sequence features, and revealing evolution.