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1] Vegetative reproduction
2]Sexual reproduction
3]Asexual reproduction
Reproduction in angiosperms
Vegetative Reproduction
• Natural vegetative propagation
• By –
• Underground stem
• Sub-aerial stem
• Roots
• Leaves
Underground stems
• Rhizome
Tuber
Bulb
Corm
Sub-aerial stem
• Runner
Offset
Sucker
• Stolon
• Vegetative reproduction
by Leaves
Ex. Brayophyllum
[Patharkuchi ],Begonia [Berki]
Kelanchoe[अमर पोई,
ज़ख़्महयात, Kop pata
• By bulbils
Artificial vegetative propagation
• Cutting
• Layering
• Grafting
• Tissue culture
[micropropagation]
Cutting
• Portion of root ,stem ,and leaf [cuttings].
• These cuttings are rooted in the soil which develop
into new plant .
• Soft wood cuttings
• Hard wood cuttings
• Examples:-
Sugarcane , roses, grapes, tapioca, coleus,
Bougainvillea.
Layering
• Roots are indused on branches still attached to
the parent plant.
• Examples :- fruit trees , ornamental plants
Layering
Layering
• 1]Mound Layering
Air layering
Grafting
1] Scion grafting
a] Whip or tongue grafting
b]Wedge grafting
c]crown grafting
2] Bud grafting
1] Scion grafting [Whip or tongue grafting]
2] Wedge grafting
Wedge grafting
Crown grafting
Bud grafting
Tissue culture
[micropropagation]
Plant tissue culture is a collection of techniques
used to maintain or grow plant cells, tissues or
organs under sterile conditions on a nutrient
culture medium of known composition. Plant
tissue culture is widely used to produce clones
of a plant in a method known as
micropropagation.
Totipotency
• Cellular totipotency is defined as the
ability of somatic cells of a plant to
produce a new complete plant. The
first evidence of cellular totipotency
was given by Steward in 'carrot
culture' experiment.
Pluripotency
• Pluripotent is also described as
something that has no fixed
developmental potential, as in being
able to differentiate into different
cell types in the case of pluripotent
stem cells.
• Equipments in tissue culture
• Autoclave
• Centrifuge machine
• Laminar airflow
• Tissue culture lab
Autoclave
Centrifuge machine
Laminar air flow
Tissue culture lab
Work under sterilised condition
CALLUS
Growth of plant [on nutrient medium]
Changing the nutrient media
Steps in tissue culture
1]Selection of explant
2]Sterlisation
3]Inoculation
4]Callus formation
5]Sub-Culturing
6]Transplantation of the Regenerated Plant
• Importance of vegetative propagation
• Rapid ,easier ,less expensive
• Specially for –
• 1]poor seed viability
• 2] prolonged seed dormancy
• 3]plants those who have lost their capacity to
produce seeds through sexual reproduction.
[banana ,seedless grapes , oranges, rose etc.]
• 4]highly heterozygous plants.
• Plants raised through vegetative reproduction
are exact genetic copies of parent.
• For economically important plants
• For raising disease free plant
• Yield of fruits can be increased by grafting
• Limitations of vegetative propagation
• Do not have tap roots .
• Not helpful in developing new varieties
Important technical terms in the description of
flower
• Complete flower Incomplete flower
• Perfect/bisexual Imperfect /unisexual
• Staminate Pistillate
• Monoecious [both flowers on same plant]
• birch, hazelnut, oak, pine, spruce, corn.
• Dioecious [when on different plants]
• asparagus, dates, mulberry, ginkgo, currant
bushes, juniper bushes ,spinach.
• Polygamous [when both bisexual and unisexual
flowers are present on same plant.]
Monoecious
plant
Relative position of floral parts on the
thalamus
brassica rose Cucumber,apple
Symmetry
Aestivation
Corolla shapes
Shapes of corolla
1]cruciform
2]Caryophyllaceous
claws and limbs right angle to each other
Rosaceous
petals not divided into claws & limbs
Campanulate
[bell shaped ]
Tubular
[tube like]
Infundibuliform
Rotate
Papillionaceous
Bilabiate
Personate
Ligulate
Urceolate
Cohesion of stamens
cucurbita Sunflower Bombax Pea Hibiscus
synandrous
syngenesious
polyadelphous
diadelphous
monoadelphous
Length of stamen
Occimum
Brassica
Adhesion of stamen
Epiphyllous- perianth
petals
sunflower
liliaceae
Gynandrous stamen-calotropis
Number of lobes in anther
Attachment of filament to anther
Dehiscence of anther
• Extrose :- an anther dehiscing towards the
periphery of the flower
• Ex. Argemone
• Introse :- an anther dehiscing towards the
centre of the flower
• Ex. Dianthus
Cohesion of carpels
Number of locules in ovary
Placentation
• Life Span :-
• Life span may be defined as a period from
birth to natural death of an organism .
• Natural death is a last event of ageing .
Life span of
• May fly – 24 hrs to 1 day banyan tree – 200 to 300 yrs
• Sequoia – 3000 to 4000 yrs Butter fly – 1 -2 weeks
• Fruit fly – 30 days Crow – 15 yrs
• Dog – 14 to 20 yrs Horse – 60 yrs
• Crocodile – 60 yrs Elephant – 60 to 90 yrs
• Parrot – 140 yrs Tortoise – 100 to 250 yrs
• Cow – 20 to 25 yrs Rice plant – 3 to 4 months
• Rose bush – 5 to 7 yrs Banana tree – 25 yrs.
• Banyan tree – 200 yrs. Sequoia – 3000 to 4000 yrs
• Actually, single celled organisms are
considered to be biologically immortal. This is
because they don't die as they grow old. They
usually undergo Mitosis (asexual
reproduction) to reproduce, in which the
organism itself gets divided into two (cell
division). ... So there is no way we could say
that the organism has died.
• (a) Juvenile phase: It is the period of growth
in an individual organism after its birth and
before it reaches reproductive maturity.
• (b) Reproductive phase: It is the period when
an individual organism reproduces sexually.
• (c) Senescent phase: It is the period when an
organism grows old and loses the ability to
reproduce.
Bambusa vulgaris
Unusual
flowering
Bamboo
Life span : 120 yrs
Flower once in a life time
• Continuous breeders
• E.g., Poultry, Rabbit, Cattle
• seasonal breeders
• E.g., Frogs, Lizards
• house fly
• Fruit fly
• Butter fly
• Human
• Rat
• Maize
• Apple
• Onion
• Rice
ophioglossum
1260 chromosomes
Rafflesia arnoldii This rare flower is found in the rainforests of
Indonesia. It can grow to be 3 feet across and
weigh up to 15 pounds! It is a parasitic plant, with
no visible leaves, roots, or stem.
Amorphophallus titanum
One of the world's tallest flowers bloomed at the Niagara Parks
Commission's Floral Showhouse . The Amorphophallus titanum, or
Titan Arum, is a massive bell-shaped flower, growing up to three
metres in height. Aside from its size, the flower is also known for
its unique smell.Jun
Flower that blossom after 12 yrs
• Strobilanthes kunthianus. Kurinji or
Neelakurinji (Strobilanthes kunthianus) is a
shrub that is found in the shola forests of the
Western Ghats in South India. Nilgiri Hills,
which literally means the blue mountains, got
their name from the purplish blue flowers of
Neelakurinji that blossoms only once in 12
years.
Strobilanthes kunthianus
Inflorescence
• Racemose
• 1]main axis elongated
a]raceme b]spike
c]catkin d]spadix
• 2] main axis shortened
a]corymb
b] umbel
• 3] main axis flattened
a]head of capitulum
Ex sunflower
Banana
Large bract
Main axis flatened
Cymose inflorescence:-
1] monochasial cyme
Ex.-begonia
2]dichasial cyme
Ex-jasmine
3]multichasial cyme
Ex .-calotropis
Special inflorescence
Hypanthodium
Ex.-Ficus
Cyathium
Poinsettia
Verticillaster
colius ,occimum
Vertisilastor
Steps in development of
microsporangia
• 1. Development of anther (microsporangium)
• 2. Formation of microspore (pollen grain) –
microsporogenesis
• 3. Pollination
• 4. Development in microspore to form mature
male gametophyte .
Structure of mature pollen grain
Development
• Zostera
• The plant known to have the longest pollen
grain till now is Zostera, with pollen grains up
to 2500 µm long.
• Pollen grains of pines, firs, and spruces are
winged.
• The smallest pollen grain, that of the forget-
me-not (Myosotis spp.), is around 6 µm
(0.006 mm) in diameter.
• In most water pollinated plants, the pollen
grains are long and ribbon like and are
protected from wetting by mucilaginous
covering. Ex. Sea grass.
Development of female gametophyte
Pollination
• Pollination is the act of transferring pollen
grains from the male anther of a flower to the
female stigma.
Agencies of pollination
By water
By wind
By bird
By bats
Self pollination
Autogamy Geitonogamy
[pollen grains are [pollen grains of one
transferred to the flower transferred
Stigma of same flower] to the stigma of
different flower of
same plant]
Contrivances or adaptations for self -
pollination
• Homogamy :- anthers and stigma of the
flower mature at the same time .
• Cleistogamy :- these flowers are bound to be
pollinated by pollens of the same flower
Contrivances or adaptations for cross
pollination
1] self sterility :-[self incompatibility]
When pollen of the same flower falls on the
stigma of the same flower fails to grow and
prevents self pollination.
2]Dichogamy :-maturation of pollen and stigma
at different time .
protogyny-ex.-Magnolia , aristolochia ,
scrophularia
Protoandry –ex.-salvia ,gossipium,helianthus
3] Herkogamy
Structure of anther and style are in such manner
that it prevents self pollination
4]Heterostyly
Long styled flower Primula vulgaris
Short styled flower
Extraordinary mode of pollination in fig
[Ficus carica]COEVOLUTION
[Blastophaga psenes]
Fertilization
FERTILIZATION
Pollen –pistil interaction
Germination of pollen grain and
growth of pollen tube
Entry of pollen tube into ovule
Routes for the entry of pollen tube
Post fertilization changes
Post fertilisation structure and events
• Development of endosperm
• 1]free nuclear
• 2]cellular
• 3]helobial
Types of endosperms
Nuclear
Cellular
Helobial
Development of zygote into an
embryo
[till formation of seed ]
• Embryogeny :- similar to both dicot and
monocot .
• Different stages on the basis of shape and
number of cells
• Then formation of epicotyl ,plumule and
cotyledons.
• Formation of testa.
Structure of dicot & monocot embryo
Seed
• It is a mature fertilized ovule that possess an
embryo ,stored food material in the
cotyledons or endosperm and a protective
seed coat .
• Integuments --develop into seed coats.
• Egg cell --grows into an embryo.
• Triploid nucleus-- gives rise to endosperm.
Types of seeds
1]Endospermic [albuminous]
2]Non-endospermic [ex-albuminous]
Advantages of seed
• Adaptive strategies for dispersal
• Sufficient food reserves
• Protection
• New genetic combination
• Variation
• Seed dormancy
• Viability
• Oldest viable seed :-lupine [Lupinus arcticus ]
[excavated from Arctic Tundra –seed germinated
and flowered after 10000 years of dormancy]
Date palm:-[Phoenix dactylifera]
2000 yrs old viable seed.
Seed dormancy can be broken by various
means
Formation of fruit
• True fruit
• False fruit
• Parthenocarpic fruit
Wall of the fruit
• Pericarp –
– Epicarp--- outer skin
– Mesocarp ---- edible fleshy region
– Endocarp ------ innermost hard region that
encloses seed
Epicarp/
True fruit
Development from ripened ovary [exclusively from ovary]
Seed develops----ovary wall matures ----floral
parts wither away.
Ex.mango ,coconut,cucumber,tomato,pea.
False fruit
• Pulpy ,edible portion is not the
wall of an ovary ,but the floral
parts like receptacle.
• Ex. –apple & strawberry
FALSE FRUIT
Parthenocarpic fruit
• Fruit develops without fertilisation
[seedless]
• Naturally or artificially
• Ex. Banana ,grapes ,oranges ,
watermelon
Kinds of fruit
Simple aggregate multiple
Fleshy dry
Dehiscent indehiscent
Aggregate fruit
Mango tomato orange cucumber apple
Types of simple fruits
dry
dehiscent
गोमेटी
bahoti
Depend on the condition of pericarp
Apomixis [agamospermy]
• Seeds are formed without fusion of
gmts.
• Diploid cells of ovule-forms nucellus
/or megaspore develops into an
embryo.
• Apomict – such plant
Parthenogenesis
• Special type of apomixis –
• Seeds develop from unfertilised
female gmt.
• Seeds could be haploid or diploid.
Parthenocarpy
• Development of fruit from the ovary without
fertilisation
• Unfertilised ovary—no ovules– no seeds in
fruit – seedless fruit
• Gustafon – first to discover parthenocarpy
• Naturally occur in– pineapple ,grapes ,apple
,pear,banana.
Sporophytic budding and polyembryony
• 1] development of embryo from sporophytic
part of the plant –like cells of nucellus or
integuments.
2]polyembryony –many embryos in the same
seed –reported by Leewenhoek
Reasons for polyembryony
• --more than one egg cell
• --more than one embryo sac in ovule.
• ---all the egg cells get fertilised
• ---number of embryos develop simultaneously
from diff. Parts of ovule like –synergids
,antipodal cells
• Number of embryos develop from the tissue
of nucellus and integuments.
• Ex. conifers, onion, groundnut, mango lemon,
orange
End of reproduction in angiosperms
Prepared by :-
Kalpana Wagh

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