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REPROCESSING OF UPPER TRACT
ENDOSCOPIC INSTRUMENTS
​Dr Mayank Mohan Agarwal
MS, MRCS(Ed), ​DNB, MCh (PGI, Chd)
​VMMF and IAUA Fellowships Uro-Oncology, Pelvic Floor Reconstruction
(MSKCC, NY; UCLA, LA; WFUBMC, NC)​
Formerly, Associate Professor of Urology (PGI, Chd)
Formerly, Consultant & Head of Urology (NMCSH, Abu Dhabi)
Senior Consultant and Head of Urology
Aster Ramesh Hospitals
Guntur (AP), India
<mayank.mohan@rameshhospitals.com>
Introduction
• Control of environment
• Control of personnel – patient & HCW
• Control of equipment
• Disinfection / sterilization
• Instrument reprocessing – principles
Sterilization and disinfection
Enveloped
viruses
Vegetative
Bacteria
Fungi Mycobacteria Nonenveloped
viruses
spores
Sterilizant
+ + + + + +
HL Disinfectant
+ + + + + +/-
IL Disinfectant
+ + + + - -
LL disinfectant
+ + +/- - - -
Rutala et al. CDC Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008
Spaulding Classification
Spaulding Classification
STEAM STERILIZATION
• The best form of sterilization whenever permissible
• 1210C 15min
• 1340C 5min
• Open instruments
• Laparoscopic instruments
• Modern telescopes
• Long channels
Flash sterilization –
• Not a replacement
• Do not pack instruments
ETHYLENE OXIDE (ETO)
• Sterilizant for moisture and heat sensitive instruments
• Gas concentration (450 to 1200 mg/l)
• Temperature (37 to 63°C)
• Relative humidity (40 to 80%)
• Exposure time (1 to 6 hours)
• Plastic wrapped stuff
• Long channels
• Problem –
• Flammable
• Environmental pollution
• Neurotoxic and carcinogenic
ETHYLENE OXIDE (ETO)
• Sterilizant for moisture and heat sensitive instruments
• Gas concentration (450 to 1200 mg/l)
• Temperature (37 to 63°C)
• Relative humidity (40 to 80%)
• Exposure time (1 to 6 hours)
• Plastic wrapped stuff
• Long channels
• Problem –
• Flammable
• Environmental pollution
• Neurotoxic and carcinogenic
125cm length, 3mm diameter –
NOT EFFECTIVE
40cm lengh, 1mm diameter -
EFFECTIVE
STERRAD® : H2O2 plasma
• Sterilization of heat sensitive instruments
• Temperature <55°C
• Exposure time <60 min
• Advantage –
• Short cycle
• Non-toxic effluent
• Efficacy against PRION
• Disadvantage –
• Luminal instruments
STERRAD® : H2O2 plasma
• Sterilization of heat sensitive instruments
• Temperature <55°C
• Exposure time <60 min
• Advantage –
• Short cycle
• Non-toxic effluent
• Efficacy against PRION
• Disadvantage –
• Luminal instruments
STERRAD® : H2O2 plasma
• Sterilization of heat sensitive instruments
• Temperature <55°C
• Exposure time <60 min
• Advantage –
• Short cycle
• Non-toxic effluent
• Efficacy against PRION
• Disadvantage –
• Luminal instruments
CHEMICAL IMMERSION
Gluteraldehyde O-pthalaldehyde Peracetic acid
Level of disinfection HLD HLD HLD / Sterilizant
Contact time for HLD 20 - 45 min 10 – 12 min 5 min
Contact time for
sterilization
10 hours 24 hours 10 min
Specialized
ventilation
Required Required Not required
Toxic to environment +++ + -
Neutralization 25g/g GLYCINE 25g/g GLYCINE NA
Rutala et al. CDC Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008
FORMALIN CHAMBER
• No standardization
• How many pills? May be 10-15 tabs
• How frequent changes? May be 7-10 days
• How much time? At least 24hrs for disinfection, 96hrs for sterilization
• Penetration into lumens – uniformly poor
• Pulmonary toxicity
• carcinogenicity
Suzuki A, Namba Y. Nagoya J Med Sci 1982
FORMALIN CHAMBER
• No standardization
• How many pills? May be 10-15 tabs
• How frequent changes? May be 7-10 days
• How much time? At least 24hrs for disinfection, 96hrs for sterilization
• Penetration into lumens – uniformly poor
• Pulmonary toxicity
• carcinogenicity
Suzuki A, Namba Y. Nagoya J Med Sci 1982
INSTRUMENT RE-PROCESSING
INSTRUMENT RE-PROCESSING
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
ENZYME SOLUTION
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
FLUSH
BRUSH
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
BRUSH
BRUSH
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION : LIQUID
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION : LIQUID
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION : GAS
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
AIR FLUSH
• DISMANTLING
• PRECLEANING
• LEAK TESTING
• MANUAL CLEANING
• RINSING AND DRYING
• DISINFECTION
• RINSING AND DRYING
• ALCOHOL FLUSH
• STORAGE / USAGE
INSTRUMENT RE-PROCESSING
Conclusion
• Instrument mechanical cleaning is A MUST (not an option)
• Never forget to FLUSH and BRUSH the channel
• Sterilization and HLD is not a substitute of mechanical cleaning
• Do not cut corners in immersion time
Conclusion
• Instrument mechanical cleaning is A MUST (not an option)
• Never forget to FLUSH and BRUSH the channel
• Sterilization and HLD is not a substitute of mechanical cleaning
• Do not cut corners in immersion time
Thank you

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REPROCESSING URETERORENOSCOPE

  • 1. REPROCESSING OF UPPER TRACT ENDOSCOPIC INSTRUMENTS ​Dr Mayank Mohan Agarwal MS, MRCS(Ed), ​DNB, MCh (PGI, Chd) ​VMMF and IAUA Fellowships Uro-Oncology, Pelvic Floor Reconstruction (MSKCC, NY; UCLA, LA; WFUBMC, NC)​ Formerly, Associate Professor of Urology (PGI, Chd) Formerly, Consultant & Head of Urology (NMCSH, Abu Dhabi) Senior Consultant and Head of Urology Aster Ramesh Hospitals Guntur (AP), India <mayank.mohan@rameshhospitals.com>
  • 2. Introduction • Control of environment • Control of personnel – patient & HCW • Control of equipment • Disinfection / sterilization • Instrument reprocessing – principles
  • 3. Sterilization and disinfection Enveloped viruses Vegetative Bacteria Fungi Mycobacteria Nonenveloped viruses spores Sterilizant + + + + + + HL Disinfectant + + + + + +/- IL Disinfectant + + + + - - LL disinfectant + + +/- - - - Rutala et al. CDC Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008
  • 6. STEAM STERILIZATION • The best form of sterilization whenever permissible • 1210C 15min • 1340C 5min • Open instruments • Laparoscopic instruments • Modern telescopes • Long channels Flash sterilization – • Not a replacement • Do not pack instruments
  • 7. ETHYLENE OXIDE (ETO) • Sterilizant for moisture and heat sensitive instruments • Gas concentration (450 to 1200 mg/l) • Temperature (37 to 63°C) • Relative humidity (40 to 80%) • Exposure time (1 to 6 hours) • Plastic wrapped stuff • Long channels • Problem – • Flammable • Environmental pollution • Neurotoxic and carcinogenic
  • 8. ETHYLENE OXIDE (ETO) • Sterilizant for moisture and heat sensitive instruments • Gas concentration (450 to 1200 mg/l) • Temperature (37 to 63°C) • Relative humidity (40 to 80%) • Exposure time (1 to 6 hours) • Plastic wrapped stuff • Long channels • Problem – • Flammable • Environmental pollution • Neurotoxic and carcinogenic 125cm length, 3mm diameter – NOT EFFECTIVE 40cm lengh, 1mm diameter - EFFECTIVE
  • 9. STERRAD® : H2O2 plasma • Sterilization of heat sensitive instruments • Temperature <55°C • Exposure time <60 min • Advantage – • Short cycle • Non-toxic effluent • Efficacy against PRION • Disadvantage – • Luminal instruments
  • 10. STERRAD® : H2O2 plasma • Sterilization of heat sensitive instruments • Temperature <55°C • Exposure time <60 min • Advantage – • Short cycle • Non-toxic effluent • Efficacy against PRION • Disadvantage – • Luminal instruments
  • 11. STERRAD® : H2O2 plasma • Sterilization of heat sensitive instruments • Temperature <55°C • Exposure time <60 min • Advantage – • Short cycle • Non-toxic effluent • Efficacy against PRION • Disadvantage – • Luminal instruments
  • 12. CHEMICAL IMMERSION Gluteraldehyde O-pthalaldehyde Peracetic acid Level of disinfection HLD HLD HLD / Sterilizant Contact time for HLD 20 - 45 min 10 – 12 min 5 min Contact time for sterilization 10 hours 24 hours 10 min Specialized ventilation Required Required Not required Toxic to environment +++ + - Neutralization 25g/g GLYCINE 25g/g GLYCINE NA Rutala et al. CDC Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008
  • 13. FORMALIN CHAMBER • No standardization • How many pills? May be 10-15 tabs • How frequent changes? May be 7-10 days • How much time? At least 24hrs for disinfection, 96hrs for sterilization • Penetration into lumens – uniformly poor • Pulmonary toxicity • carcinogenicity Suzuki A, Namba Y. Nagoya J Med Sci 1982
  • 14. FORMALIN CHAMBER • No standardization • How many pills? May be 10-15 tabs • How frequent changes? May be 7-10 days • How much time? At least 24hrs for disinfection, 96hrs for sterilization • Penetration into lumens – uniformly poor • Pulmonary toxicity • carcinogenicity Suzuki A, Namba Y. Nagoya J Med Sci 1982
  • 17. INSTRUMENT RE-PROCESSING • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE
  • 18. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 19. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 20. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 21. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 22. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING ENZYME SOLUTION
  • 23. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING FLUSH BRUSH
  • 24. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING BRUSH BRUSH
  • 25. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 26. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 27. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION : LIQUID • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 28. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION : LIQUID • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 29. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION : GAS • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 30. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 31. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING AIR FLUSH
  • 32. • DISMANTLING • PRECLEANING • LEAK TESTING • MANUAL CLEANING • RINSING AND DRYING • DISINFECTION • RINSING AND DRYING • ALCOHOL FLUSH • STORAGE / USAGE INSTRUMENT RE-PROCESSING
  • 33. Conclusion • Instrument mechanical cleaning is A MUST (not an option) • Never forget to FLUSH and BRUSH the channel • Sterilization and HLD is not a substitute of mechanical cleaning • Do not cut corners in immersion time
  • 34. Conclusion • Instrument mechanical cleaning is A MUST (not an option) • Never forget to FLUSH and BRUSH the channel • Sterilization and HLD is not a substitute of mechanical cleaning • Do not cut corners in immersion time