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Essential
Asepsis &
Sterilization
Practical Suggestions for a clean practice
DR.CHITRA CHAKRAVARTHY
PROF. & HOD DEPT OF OMFS
NAVODAYA DENTAL COLLEGE
The 4 areas of
interest
DENTAL INSTRUMENTS
DENTAL UNIT
ENVIRONMENTAL SURFACES
DENTAL LABORATORY
Classification based on
risk of transmission
CRITICAL
SEMI-CRITICAL
NON-CRITICAL
Penetrate MUCOUS MEMBRANES or
CONTACT BONE, BLOODSTREAM, or
other normally sterile tissues.
HEAT STERILIZE between uses or use
sterile single- use, DISPOSABLE
devices.
Examples include SURGICAL
INSTRUMENTS, SCALPEL BLADES,
PERIODONTAL SCALERS, AND
SURGICAL DENTAL BURS
Classification based on
risk of transmission
CRITICAL
SEMI-CRITICAL
NON-CRITICAL
Contact MUCOUS
MEMBRANES but do NOT
PENETRATE SOFT TISSUE
HEAT STERILIZE or HIGH-
LEVEL DISINFECT
Examples: DENTAL MOUTH
MIRRORS, AMALGAM
CONDENSERS, AND
DENTAL HANDPIECES
Classification based on
risk of transmission
CRITICAL
SEMI-CRITICAL
NON-CRITICAL
Contact intact SKIN
Clean and disinfect
using a LOW TO
INTERMEDIATE LEVEL
DISINFECTANT
Examples: X-RAY
HEADS, FACE BOWS,
PULSE OXIMETER,
BLOOD PRESSURE
METHODS OF STERILIZATION
HEAT CHEMICAL RADIATION
BOILING WATER CHEMICLAVE
IONIZING
RADIATION
HOT AIR OVENS ETHYLENE OXIDE GAS
STEAM AUTOCLAVE
HEAT STERILIZATION
PRE SEALED AUTOCLAVABLE POUCHES
AUTOCLAVE - COOKER TYPE, B CLASS, C CLASS
HEAT STERILIZATION - AUTOCLAVE
Sterilization with STEAM UNDER PRESSURE
Time required at 1210 C is 15 mins at 15 lbs of
pressure.
Advantages
† Rapid and effective
† Effective for sterilizing cloth surgical packs and towel
packs
Disadvantages
† Items sensitive to heat cannot be sterilized
† It tends to corrode carbon steel burs and instruments
DRY HEAT STERILIZATION
Dry Heat Sterilization by CONVENTIONAL DRY HEAT OVENS.
Achieved at temperature above 1600 C.
Have heated chambers that allow air to circulate by gravity
flow.
6-12mins is required for sterilization
Disadvantages
Without careful calibration, more chances sterilization failures
The most accurate way to calibrate a sterilization cycle is by
using external temperature gauge (pyrometer) attached to a
thermocouple wire.
CHEMICAL STERILIZATION
Operates at 1310 C and 20 lbs of pressure.
They have a cycle time of half an hour.
Advantages
† Carbon steel and other carbon sensitive burs, instruments
and pliers are sterilized without rust or corrosion
Disadvantages
† Items sensitive to elevated temperature will be damaged
† Instruments must be very lightly packed.
† Towel and heavy clothing cannot be sterilized.
STERILIZATION MONITORING
Types of Indicators
Mechanical
† Measure time
† Temperature
† Pressure
Chemical
† Change in color when physical parameter is reached
Biological (spore tests)
† Use biological spores to assess the sterilization
process directly
DENTAL UNIT
DISINFECTION
Cleaned by DISPOSABLE TOWELING
Use an EPA registered hospital
disinfectant
(EPA-Environmental Protective Agency)
Cleaning Agents Like PHENOLICS,
IODOPHORS, CHLORINE containing
compounds
ENVIRONMENTAL
SURFACES DISINFECTION
CLINICAL CONTACT SURFACES
High potential for DIRECT
CONTAMINATION from spray or spatter or
by contact with gloved hand.
HOUSEKEEPING SURFACES
Do not come into contact with patients or
devices –LIMITED RISK of disease
transmission
CLINICAL CONTACT SURFACES
LIGHT
HANDLES
HANDPIECE
HANDLES
INSTRUMENT
TROLLEY
CHAIR
SURFACES
SPITOON
MICROSCOPE
HANDLES
CLINICAL CONTACT SURFACES
Risk of transmitting infections greater than for
housekeeping surfaces.
Surface barriers can be used and changed
between patients.
OR
Clean, then disinfect using an EPA-registered
low- (HIV/HBV claim) to intermediate-level
(tuberculocidal claim) hospital disinfectant.
HOUSEKEEPING SURFACES
WASH
BASINS
STOOLS
STORAGE
CABINETS
HOUSEKEEPING SURFACES
Routinely clean with SOAP AND WATER or
an EPA- REGISTERED
DETERGENT/HOSPITAL DISINFECTANT
routinely
Clean MOPS AND CLOTHS and allow to
dry thoroughly before re-using.
Prepare FRESH CLEANING AND
DISINFECTING SOLUTIONS daily and per
manufacturer recommendations.
DENTAL PLASTER LAB
Need COORDINATION between DENTAL OFFICE AND LAB
Use of proper methods/materials for handling and
decontaminating soiled incoming items
All contaminated INCOMING ITEMS should be cleaned and
DISINFECTED before being HANDLED BY LAB PERSONNEL,
and before being returned to the patient
DENTAL PLASTER LAB
AS A RULE ALLOW ONLY
BIOLOGICALLY CLEAN ITEMS ONLY
A SIGN AND MONITOR SYSTEM BE
IMPLEMENTED
DENTAL PLASTER LAB
INCOMING ITEMS
† Rinse under running tap water to remove blood/saliva
† Use ultrasonic cleaners to remove macro debris
† Disinfect as appropriate
† Rinse thoroughly with tap water to remove residual
disinfectant
† No single disinfectant is ideal or compatible with all
items
DENTAL PLASTER LAB
OUTGOING ITEMS
† Clean and disinfect before delivery
to patient
† After disinfection: rinse and place
in plastic bag with diluted
mouthwash until insertion
† Do not store in disinfectant before
insertion
† Label the plastic bag: “This case
shipment has been disinfected with
______ for _____ minutes”
In “Cleanliness” lies “Godliness”

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Basic Sterilization & Asepsis.pptx

  • 1. Essential Asepsis & Sterilization Practical Suggestions for a clean practice DR.CHITRA CHAKRAVARTHY PROF. & HOD DEPT OF OMFS NAVODAYA DENTAL COLLEGE
  • 2. The 4 areas of interest DENTAL INSTRUMENTS DENTAL UNIT ENVIRONMENTAL SURFACES DENTAL LABORATORY
  • 3. Classification based on risk of transmission CRITICAL SEMI-CRITICAL NON-CRITICAL Penetrate MUCOUS MEMBRANES or CONTACT BONE, BLOODSTREAM, or other normally sterile tissues. HEAT STERILIZE between uses or use sterile single- use, DISPOSABLE devices. Examples include SURGICAL INSTRUMENTS, SCALPEL BLADES, PERIODONTAL SCALERS, AND SURGICAL DENTAL BURS
  • 4. Classification based on risk of transmission CRITICAL SEMI-CRITICAL NON-CRITICAL Contact MUCOUS MEMBRANES but do NOT PENETRATE SOFT TISSUE HEAT STERILIZE or HIGH- LEVEL DISINFECT Examples: DENTAL MOUTH MIRRORS, AMALGAM CONDENSERS, AND DENTAL HANDPIECES
  • 5. Classification based on risk of transmission CRITICAL SEMI-CRITICAL NON-CRITICAL Contact intact SKIN Clean and disinfect using a LOW TO INTERMEDIATE LEVEL DISINFECTANT Examples: X-RAY HEADS, FACE BOWS, PULSE OXIMETER, BLOOD PRESSURE
  • 6. METHODS OF STERILIZATION HEAT CHEMICAL RADIATION BOILING WATER CHEMICLAVE IONIZING RADIATION HOT AIR OVENS ETHYLENE OXIDE GAS STEAM AUTOCLAVE
  • 7. HEAT STERILIZATION PRE SEALED AUTOCLAVABLE POUCHES AUTOCLAVE - COOKER TYPE, B CLASS, C CLASS
  • 8. HEAT STERILIZATION - AUTOCLAVE Sterilization with STEAM UNDER PRESSURE Time required at 1210 C is 15 mins at 15 lbs of pressure. Advantages † Rapid and effective † Effective for sterilizing cloth surgical packs and towel packs Disadvantages † Items sensitive to heat cannot be sterilized † It tends to corrode carbon steel burs and instruments
  • 9. DRY HEAT STERILIZATION Dry Heat Sterilization by CONVENTIONAL DRY HEAT OVENS. Achieved at temperature above 1600 C. Have heated chambers that allow air to circulate by gravity flow. 6-12mins is required for sterilization Disadvantages Without careful calibration, more chances sterilization failures The most accurate way to calibrate a sterilization cycle is by using external temperature gauge (pyrometer) attached to a thermocouple wire.
  • 10. CHEMICAL STERILIZATION Operates at 1310 C and 20 lbs of pressure. They have a cycle time of half an hour. Advantages † Carbon steel and other carbon sensitive burs, instruments and pliers are sterilized without rust or corrosion Disadvantages † Items sensitive to elevated temperature will be damaged † Instruments must be very lightly packed. † Towel and heavy clothing cannot be sterilized.
  • 11. STERILIZATION MONITORING Types of Indicators Mechanical † Measure time † Temperature † Pressure Chemical † Change in color when physical parameter is reached Biological (spore tests) † Use biological spores to assess the sterilization process directly
  • 12. DENTAL UNIT DISINFECTION Cleaned by DISPOSABLE TOWELING Use an EPA registered hospital disinfectant (EPA-Environmental Protective Agency) Cleaning Agents Like PHENOLICS, IODOPHORS, CHLORINE containing compounds
  • 13. ENVIRONMENTAL SURFACES DISINFECTION CLINICAL CONTACT SURFACES High potential for DIRECT CONTAMINATION from spray or spatter or by contact with gloved hand. HOUSEKEEPING SURFACES Do not come into contact with patients or devices –LIMITED RISK of disease transmission
  • 15. CLINICAL CONTACT SURFACES Risk of transmitting infections greater than for housekeeping surfaces. Surface barriers can be used and changed between patients. OR Clean, then disinfect using an EPA-registered low- (HIV/HBV claim) to intermediate-level (tuberculocidal claim) hospital disinfectant.
  • 17. HOUSEKEEPING SURFACES Routinely clean with SOAP AND WATER or an EPA- REGISTERED DETERGENT/HOSPITAL DISINFECTANT routinely Clean MOPS AND CLOTHS and allow to dry thoroughly before re-using. Prepare FRESH CLEANING AND DISINFECTING SOLUTIONS daily and per manufacturer recommendations.
  • 18. DENTAL PLASTER LAB Need COORDINATION between DENTAL OFFICE AND LAB Use of proper methods/materials for handling and decontaminating soiled incoming items All contaminated INCOMING ITEMS should be cleaned and DISINFECTED before being HANDLED BY LAB PERSONNEL, and before being returned to the patient
  • 19. DENTAL PLASTER LAB AS A RULE ALLOW ONLY BIOLOGICALLY CLEAN ITEMS ONLY A SIGN AND MONITOR SYSTEM BE IMPLEMENTED
  • 20. DENTAL PLASTER LAB INCOMING ITEMS † Rinse under running tap water to remove blood/saliva † Use ultrasonic cleaners to remove macro debris † Disinfect as appropriate † Rinse thoroughly with tap water to remove residual disinfectant † No single disinfectant is ideal or compatible with all items
  • 21. DENTAL PLASTER LAB OUTGOING ITEMS † Clean and disinfect before delivery to patient † After disinfection: rinse and place in plastic bag with diluted mouthwash until insertion † Do not store in disinfectant before insertion † Label the plastic bag: “This case shipment has been disinfected with ______ for _____ minutes”
  • 22. In “Cleanliness” lies “Godliness”