This standard operating procedure summarizes the safety protocols for operating the BD FACSARIA III cell sorter. Personal protective equipment like gloves and safety glasses are required. Potential hazards include exposure to cells and electricity from deflection plates. The document provides detailed instructions on starting up and shutting down the device, installing nozzles, running tests and cleaning procedures. Waste disposal and emergency response procedures are also outlined.
This document discusses infection control practices in operating rooms for ophthalmology. It covers possible infections that can occur, issues with current practices, operating room etiquette, planning and designing the operating room complex layout, caring for instruments, cleaning and sterilization/disinfection of operating rooms. The operating room is the most critical area of the hospital and cleanliness is important for patient safety. A systematic approach by the operating team following proper protocols can help reduce infections.
10 don't miss practical summary for your safetyaakaricls
WHY YOU NEED TO DO THIS COURSE?
You are doctors and are well aware about current scenario. You are even taking adequate care. Then why you should do this course?
1. Friends this course aims to provide general guidance and information on how to prevent the spread of COVID-19 in the workplace, to enable staff to return to work safely while keeping the risk of contamination as low as possible.
2. It also provides ideas on how to protect mental well-being during the pandemic.
3. All General Practitioners, Consultants owning their own workplace and Freelancing Consultants can get information on how to take care while restarting medical practice,
4. Happy Doctor Foundation always helps doctors. And you will agree with us that a doctor is at MORE RISK AND IS MORE VULNERABLE TO GET INFECTION! So the more you learn, more you become wise. Isn’t it?
5. Do you know that your family’s health depends on HOW WELL YOU PROTECT YOURSELF?
6. You have nothing to lose by undergoing these course modules, so why not give it a try?
by - dr. sheetal kapse, 2nd year p.g. student, dept. of oral & maxillofacial surgery, RCDSR, Bhilai, C.G. please contact for any question...email id - sheetal.kpse@yahoo.com
Sterilization and disinfection in eye hospitals asim sillionsleaders
This document discusses sterilization, disinfection, and cleaning procedures that are important for maintaining safety in eye hospitals. It provides definitions and guidelines for key concepts like sterilization, disinfection, and cleaning. Specific recommendations are given for operating theatre layout, construction, equipment, air handling, and sterilization protocols using autoclaving or chemicals. Maintaining proper sterilization and disinfection practices requires discipline from staff, leadership support, and quality assurance processes.
Infection Control Guidelines for Ophthalmology Clinic [compatibility mode]drnahla
Infection Control Guidelines for Ophthalmology Clinic
Infection Prevention in Ophthalmology Clinic
Dr. NAHLA ABDEL KADERوMD, PhD.
INFECTION CONTROL CONSULTANT, MOH
INFECTION CONTROL CBAHI SURVEYOR
Infection Control Director, KKH.
This document provides guidelines for maintaining a sterile environment in an operating theatre (OT) to prevent surgical site infections. It outlines policies for OT staff dress code and conduct, including proper hand hygiene and restricting access. The OT layout separates zones by sterility. Cleaning procedures are described for daily cleaning between surgeries, deep weekly cleaning, and handling soiled equipment and laundry. Standard infection control precautions like proper disinfectant use and spills management are also covered.
Cleaning and disinfection of p atient care equipmentMEEQAT HOSPITAL
This document provides definitions and guidelines for cleaning, disinfecting, and sterilizing patient care equipment. It outlines that cleaning removes foreign material, disinfection eliminates most pathogens, and sterilization destroys all microbes. It distinguishes between critical equipment that enters sterile tissues and non-critical equipment that touches intact skin. The goals are to eliminate transmission between patients and staff. It provides policies on sterilizing critical items and disinfecting high-touch non-critical surfaces between each patient use. Responsibilities and proper procedures are defined to ensure effective cleaning and protection of staff.
This document outlines the 9 key steps for reprocessing flexible gastrointestinal endoscopes: 1) precleaning, 2) leak testing, 3) manual cleaning, 4) rinsing after cleaning, 5) visual inspection, 6) high level disinfection, 7) rinsing after disinfection, 8) drying, and 9) storage. It emphasizes that manual cleaning is the most important step for removing debris and that all 9 steps must be followed diligently to ensure endoscope decontamination and prevent patient infection. Proper reprocessing according to manufacturer guidelines is critical for patient and staff safety.
This document discusses infection control practices in operating rooms for ophthalmology. It covers possible infections that can occur, issues with current practices, operating room etiquette, planning and designing the operating room complex layout, caring for instruments, cleaning and sterilization/disinfection of operating rooms. The operating room is the most critical area of the hospital and cleanliness is important for patient safety. A systematic approach by the operating team following proper protocols can help reduce infections.
10 don't miss practical summary for your safetyaakaricls
WHY YOU NEED TO DO THIS COURSE?
You are doctors and are well aware about current scenario. You are even taking adequate care. Then why you should do this course?
1. Friends this course aims to provide general guidance and information on how to prevent the spread of COVID-19 in the workplace, to enable staff to return to work safely while keeping the risk of contamination as low as possible.
2. It also provides ideas on how to protect mental well-being during the pandemic.
3. All General Practitioners, Consultants owning their own workplace and Freelancing Consultants can get information on how to take care while restarting medical practice,
4. Happy Doctor Foundation always helps doctors. And you will agree with us that a doctor is at MORE RISK AND IS MORE VULNERABLE TO GET INFECTION! So the more you learn, more you become wise. Isn’t it?
5. Do you know that your family’s health depends on HOW WELL YOU PROTECT YOURSELF?
6. You have nothing to lose by undergoing these course modules, so why not give it a try?
by - dr. sheetal kapse, 2nd year p.g. student, dept. of oral & maxillofacial surgery, RCDSR, Bhilai, C.G. please contact for any question...email id - sheetal.kpse@yahoo.com
Sterilization and disinfection in eye hospitals asim sillionsleaders
This document discusses sterilization, disinfection, and cleaning procedures that are important for maintaining safety in eye hospitals. It provides definitions and guidelines for key concepts like sterilization, disinfection, and cleaning. Specific recommendations are given for operating theatre layout, construction, equipment, air handling, and sterilization protocols using autoclaving or chemicals. Maintaining proper sterilization and disinfection practices requires discipline from staff, leadership support, and quality assurance processes.
Infection Control Guidelines for Ophthalmology Clinic [compatibility mode]drnahla
Infection Control Guidelines for Ophthalmology Clinic
Infection Prevention in Ophthalmology Clinic
Dr. NAHLA ABDEL KADERوMD, PhD.
INFECTION CONTROL CONSULTANT, MOH
INFECTION CONTROL CBAHI SURVEYOR
Infection Control Director, KKH.
This document provides guidelines for maintaining a sterile environment in an operating theatre (OT) to prevent surgical site infections. It outlines policies for OT staff dress code and conduct, including proper hand hygiene and restricting access. The OT layout separates zones by sterility. Cleaning procedures are described for daily cleaning between surgeries, deep weekly cleaning, and handling soiled equipment and laundry. Standard infection control precautions like proper disinfectant use and spills management are also covered.
Cleaning and disinfection of p atient care equipmentMEEQAT HOSPITAL
This document provides definitions and guidelines for cleaning, disinfecting, and sterilizing patient care equipment. It outlines that cleaning removes foreign material, disinfection eliminates most pathogens, and sterilization destroys all microbes. It distinguishes between critical equipment that enters sterile tissues and non-critical equipment that touches intact skin. The goals are to eliminate transmission between patients and staff. It provides policies on sterilizing critical items and disinfecting high-touch non-critical surfaces between each patient use. Responsibilities and proper procedures are defined to ensure effective cleaning and protection of staff.
This document outlines the 9 key steps for reprocessing flexible gastrointestinal endoscopes: 1) precleaning, 2) leak testing, 3) manual cleaning, 4) rinsing after cleaning, 5) visual inspection, 6) high level disinfection, 7) rinsing after disinfection, 8) drying, and 9) storage. It emphasizes that manual cleaning is the most important step for removing debris and that all 9 steps must be followed diligently to ensure endoscope decontamination and prevent patient infection. Proper reprocessing according to manufacturer guidelines is critical for patient and staff safety.
This document discusses methods for monitoring surgical site infections through air sampling in operating theaters. It reviews debates around whether air sampling or conventional swabbing is better. The document provides guidelines for effective air sampling, including using a slit sampler or air centrifuge to quantitatively measure bacterial colony counts. It recommends counts below 35 CFU per cubic meter for conventional theaters and 1 CFU for clean theaters. Overall it aims to establish best practices for regular air sampling to prevent surgical site infections through airborne contamination in operating theaters.
The Central Sterile Supply Department (CSSD) processes reusable medical devices for hospitals by cleaning, disinfecting, packaging, and sterilizing items. The CSSD area is divided into four sections - a dirty area for decontamination, a clean area for packaging and preparation, a linen packaging area, and a sterile storage area. The centralized CSSD model ensures staff are trained specifically for processing devices, the department is properly equipped, and quality control is maintained, improving safety.
This document discusses cleaning and decontamination procedures for medical devices. It covers the importance of cleaning as the first step in reprocessing, factors that impact the cleaning process like environmental design and staff training, and selection and use of cleaning agents. It also explains manual and mechanical cleaning methods and procedures for cleaning different types of instruments.
This document outlines procedures for cleaning, disinfecting, and sterilizing instruments and medical supplies at a hospital's Central Sterile Supply Department (CSSD). It describes collecting soiled instruments from operating rooms and consumer departments, transporting them to the CSSD, and pre-disinfecting them to reduce microorganisms. It then details the steps for thoroughly cleaning and disinfecting instruments through scrubbing and soaking in detergent solutions, rinsing, inspecting, and drying them to prepare for sterilization. The procedures are intended to ensure staff safety, minimize the spread of germs, and produce clean instruments ready for sterilization.
This presentation discusses how to properly clean and decontaminate spills of blood and other potentially infectious materials in laboratories. It defines minor and major spills and outlines the appropriate response and cleanup procedures for each. Specific protocols are provided for cleaning spills on floors, benches, in biosafety cabinets, and centrifuges. Key steps include wearing proper PPE, covering spills with absorbent materials, applying an appropriate disinfectant for 20 minutes of contact time, and proper disposal of contaminated waste. The goal is to safely and effectively decontaminate any spilled infectious materials to prevent disease transmission.
Central sterilization supply departmentAshraf selim
The document discusses concepts and procedures for central sterilization and supply departments (CSSD). It covers general planning concepts like airflow, staff flow and workflow. It also discusses criteria for CSSD areas including changing rooms, wall finishes and lighting. The document then outlines the steps for cleaning, drying, inspecting, packaging, loading and storing sterilized instruments as well as environmental parameters and storage time protocols. The overall goal is to prevent contamination and effectively reprocess reusable medical equipment to maintain sterility.
The Central Sterile Supply Department (CSSD) is responsible for receiving, processing, sterilizing, storing, and distributing medical equipment and supplies. It aims to provide safe sterile supplies and reduce hospital-acquired infections. CSSD developed from the need for aseptic techniques after the discovery of microorganisms. It has specific areas for receiving, cleaning, sterilizing, storing and distributing supplies following a one-way workflow. CSSD uses various sterilization methods like heat, ETO, radiation and chemicals depending on the item to be sterilized. Regular bacteriological testing of sterilizers is done to ensure sterility.
Aseptic Garbing, Hand Washing, and Gloving Kdurant36
The document provides an overview of aseptic garbing, hand washing, and gloving procedures according to USP Chapter <797> guidelines. It discusses learning objectives, topics that will be covered, and the importance of maintaining asepsis when preparing sterile compounded products. Specific procedures covered include self-assessment, use of personal protective equipment like gowns and gloves, essential supplies, and designated areas for aseptic hand washing. The goal is to avoid introducing pathogens while protecting both patients and healthcare workers.
This document provides information about aseptic techniques for intravenous (IV) preparation:
1) Aseptic technique involves procedures to keep sterile products contamination-free. IV is the most common parenteral route of drug administration today.
2) Risks of IV therapy include infection, air embolism, bleeding, allergic reactions, incompatibilities, extravasation, particulate contamination, and phlebitis.
3) Sterile areas and laminar flow hoods are used to maintain sterile conditions during IV preparation, with strict protocols for equipment placement, personnel movement, and cleaning.
This document outlines standards of behavior and practices for cleanrooms and cleanzones. It defines key terms like cleanroom, cleanzone, critical activity, and aseptic processing. The document provides guidelines for behaviors in all cleanrooms and cleanzones, including proper gowning, minimizing contamination, and maintaining orderly work areas. It also outlines additional requirements for aseptic filling suites, such as proper glove sanitization procedures and using aseptic technique. The document states that compliance with these standards will be assessed through microbiological audits and is necessary to meet regulatory expectations for sterile drug production.
New technologies for surfaces disinfectionAshraf selim
This document discusses new technologies for disinfecting surfaces in hospitals to help prevent healthcare-associated infections. It describes no-touch disinfection technologies like hydrogen peroxide vapor, ultraviolet light, ozone gas, and chlorine dioxide fogging that can disinfect entire rooms. It also discusses self-disinfecting surfaces like those impregnated with copper or silver, coated with light-activated antimicrobials, or having a sharklet pattern that hinders bacterial attachment. Adopting these supplemental methods along with routine cleaning could help reduce recontamination of surfaces between patients and decrease infection risks.
Central sterile supply department(cssd)anees fatima
The Central Sterile Supply Department (CSSD) is responsible for receiving, processing, sterilizing, storing and distributing sterile supplies and equipment for hospitals. Key functions of the CSSD include cleaning, packaging, sterilizing items using autoclaves or ethylene oxide, storing sterile supplies, and issuing items to different hospital units. CSSDs aim to provide safe sterile supplies efficiently while standardizing equipment and assisting in infection control.
This document provides guidance on managing spills of cytotoxic drugs. It defines a cytotoxic spill as any uncontrolled release of hazardous chemicals, whether solid, liquid, or gas. The recommended procedure is to notify personnel, isolate the spill area, contain the spill, put on protective equipment, clean up the spill using a 5% decon 90 solution, dispose of all contaminated waste properly, wash hands, and file an incident report. Spill kits should contain protective gowns, masks, gloves, waste bags, cleaning supplies, and instructions for managing cytotoxic spills.
This document provides an overview of sterile packaging and storage. It discusses the objectives of sterile packaging including protecting contents, maintaining sterility, and allowing for aseptic opening. It reviews various reusable and disposable packaging materials like muslin, pouches, and rigid containers. Proper packaging procedures are outlined including preparation, closure methods, labeling, and storage standards. Maintaining sterility through careful handling and following event-related concepts is emphasized.
The document summarizes the requirements for an IV room project based on USP <797> standards. It discusses the primary engineering controls including laminar airflow workbenches and biological safety cabinets. It also outlines the architecture of buffer and ante areas, recommended devices for compounding, cleaning and disinfecting frequency guidelines, personnel hygiene and garbing procedures, packaging and labeling standards, and staff responsibilities. The IV room is designed to meet most USP requirements, with some exceptions like not fully separating the buffer and ante areas into different rooms.
Pharmacy CSP Preparation: Personnel Garbing and GlovingJerry Fahrni
Personnel garbing and gloving procedures are outlined to minimize contamination when compounding sterile preparations. Individuals are excluded if shedding particles at high rates from infections or wounds. Garbing begins with removing jewelry and outerwear then donning shoe covers, hair/beard covers, and a mask in the ante-area. Hands and forearms are washed to the elbows for 30 seconds followed by drying and donning a gown. In the buffer area, hands are scrubbed with alcohol-based sanitizer and allowed to dry before donning sterile gloves. Gloves are disinfected throughout compounding and replaced if breached. PPE is replaced or redonned as needed to maintain sterility when reentering the compound
The document discusses the importance and functions of the Central Sterile Supply Department (CSSD) in hospitals. It outlines the history and objectives of CSSDs, which aim to provide sterilized medical supplies from a central location. The optimal design principles are described, including workflow zones and layout. Key areas like cleaning, sterilization, storage and distribution are explained. Finally, the document provides an inventory of recommended equipment for setting up an ideal CSSD.
Haldor's flagship solution, the ORLocate™ system, tracks, manages and analyses usage patterns of surgical instruments using radio frequency identification (RFID) technology. This highly modular, reliable, scalable and accurate system blends seamlessly with the workflow in the sterile processing department and during the surgical procedures. It also provides the capabilities to interact with the hospital’s real-time location system as well as hospital information systems and technologies. The ORLocate™ system is FDA 510(k) cleared and CE certified.
This document summarizes the development of a cost-effective test tube holder for a cell sorting instrument. A team created a custom device to hold test tubes of varying sizes and included a magnetic stirring plate to mix fluids during sorting, improving product collection and cell viability. Their design costs significantly less than the manufacturer's device and provides extra functionality. They successfully tested cell sorting and mixing with the new holder.
Three 3D printed models of a custom test tube holder were created for the BD FACS ARIA III cell sorting machine. Each model can hold different combinations of test tube sizes. Testing showed that a model holding two 50mL test tubes sorted cells with 88.77% accuracy, sorting different cell types into their respective test tubes. A magnetic stirring plate was attached to help mix fluids inside the test tubes. Results demonstrated that the stirring plate successfully mixed fluids together.
This document discusses methods for monitoring surgical site infections through air sampling in operating theaters. It reviews debates around whether air sampling or conventional swabbing is better. The document provides guidelines for effective air sampling, including using a slit sampler or air centrifuge to quantitatively measure bacterial colony counts. It recommends counts below 35 CFU per cubic meter for conventional theaters and 1 CFU for clean theaters. Overall it aims to establish best practices for regular air sampling to prevent surgical site infections through airborne contamination in operating theaters.
The Central Sterile Supply Department (CSSD) processes reusable medical devices for hospitals by cleaning, disinfecting, packaging, and sterilizing items. The CSSD area is divided into four sections - a dirty area for decontamination, a clean area for packaging and preparation, a linen packaging area, and a sterile storage area. The centralized CSSD model ensures staff are trained specifically for processing devices, the department is properly equipped, and quality control is maintained, improving safety.
This document discusses cleaning and decontamination procedures for medical devices. It covers the importance of cleaning as the first step in reprocessing, factors that impact the cleaning process like environmental design and staff training, and selection and use of cleaning agents. It also explains manual and mechanical cleaning methods and procedures for cleaning different types of instruments.
This document outlines procedures for cleaning, disinfecting, and sterilizing instruments and medical supplies at a hospital's Central Sterile Supply Department (CSSD). It describes collecting soiled instruments from operating rooms and consumer departments, transporting them to the CSSD, and pre-disinfecting them to reduce microorganisms. It then details the steps for thoroughly cleaning and disinfecting instruments through scrubbing and soaking in detergent solutions, rinsing, inspecting, and drying them to prepare for sterilization. The procedures are intended to ensure staff safety, minimize the spread of germs, and produce clean instruments ready for sterilization.
This presentation discusses how to properly clean and decontaminate spills of blood and other potentially infectious materials in laboratories. It defines minor and major spills and outlines the appropriate response and cleanup procedures for each. Specific protocols are provided for cleaning spills on floors, benches, in biosafety cabinets, and centrifuges. Key steps include wearing proper PPE, covering spills with absorbent materials, applying an appropriate disinfectant for 20 minutes of contact time, and proper disposal of contaminated waste. The goal is to safely and effectively decontaminate any spilled infectious materials to prevent disease transmission.
Central sterilization supply departmentAshraf selim
The document discusses concepts and procedures for central sterilization and supply departments (CSSD). It covers general planning concepts like airflow, staff flow and workflow. It also discusses criteria for CSSD areas including changing rooms, wall finishes and lighting. The document then outlines the steps for cleaning, drying, inspecting, packaging, loading and storing sterilized instruments as well as environmental parameters and storage time protocols. The overall goal is to prevent contamination and effectively reprocess reusable medical equipment to maintain sterility.
The Central Sterile Supply Department (CSSD) is responsible for receiving, processing, sterilizing, storing, and distributing medical equipment and supplies. It aims to provide safe sterile supplies and reduce hospital-acquired infections. CSSD developed from the need for aseptic techniques after the discovery of microorganisms. It has specific areas for receiving, cleaning, sterilizing, storing and distributing supplies following a one-way workflow. CSSD uses various sterilization methods like heat, ETO, radiation and chemicals depending on the item to be sterilized. Regular bacteriological testing of sterilizers is done to ensure sterility.
Aseptic Garbing, Hand Washing, and Gloving Kdurant36
The document provides an overview of aseptic garbing, hand washing, and gloving procedures according to USP Chapter <797> guidelines. It discusses learning objectives, topics that will be covered, and the importance of maintaining asepsis when preparing sterile compounded products. Specific procedures covered include self-assessment, use of personal protective equipment like gowns and gloves, essential supplies, and designated areas for aseptic hand washing. The goal is to avoid introducing pathogens while protecting both patients and healthcare workers.
This document provides information about aseptic techniques for intravenous (IV) preparation:
1) Aseptic technique involves procedures to keep sterile products contamination-free. IV is the most common parenteral route of drug administration today.
2) Risks of IV therapy include infection, air embolism, bleeding, allergic reactions, incompatibilities, extravasation, particulate contamination, and phlebitis.
3) Sterile areas and laminar flow hoods are used to maintain sterile conditions during IV preparation, with strict protocols for equipment placement, personnel movement, and cleaning.
This document outlines standards of behavior and practices for cleanrooms and cleanzones. It defines key terms like cleanroom, cleanzone, critical activity, and aseptic processing. The document provides guidelines for behaviors in all cleanrooms and cleanzones, including proper gowning, minimizing contamination, and maintaining orderly work areas. It also outlines additional requirements for aseptic filling suites, such as proper glove sanitization procedures and using aseptic technique. The document states that compliance with these standards will be assessed through microbiological audits and is necessary to meet regulatory expectations for sterile drug production.
New technologies for surfaces disinfectionAshraf selim
This document discusses new technologies for disinfecting surfaces in hospitals to help prevent healthcare-associated infections. It describes no-touch disinfection technologies like hydrogen peroxide vapor, ultraviolet light, ozone gas, and chlorine dioxide fogging that can disinfect entire rooms. It also discusses self-disinfecting surfaces like those impregnated with copper or silver, coated with light-activated antimicrobials, or having a sharklet pattern that hinders bacterial attachment. Adopting these supplemental methods along with routine cleaning could help reduce recontamination of surfaces between patients and decrease infection risks.
Central sterile supply department(cssd)anees fatima
The Central Sterile Supply Department (CSSD) is responsible for receiving, processing, sterilizing, storing and distributing sterile supplies and equipment for hospitals. Key functions of the CSSD include cleaning, packaging, sterilizing items using autoclaves or ethylene oxide, storing sterile supplies, and issuing items to different hospital units. CSSDs aim to provide safe sterile supplies efficiently while standardizing equipment and assisting in infection control.
This document provides guidance on managing spills of cytotoxic drugs. It defines a cytotoxic spill as any uncontrolled release of hazardous chemicals, whether solid, liquid, or gas. The recommended procedure is to notify personnel, isolate the spill area, contain the spill, put on protective equipment, clean up the spill using a 5% decon 90 solution, dispose of all contaminated waste properly, wash hands, and file an incident report. Spill kits should contain protective gowns, masks, gloves, waste bags, cleaning supplies, and instructions for managing cytotoxic spills.
This document provides an overview of sterile packaging and storage. It discusses the objectives of sterile packaging including protecting contents, maintaining sterility, and allowing for aseptic opening. It reviews various reusable and disposable packaging materials like muslin, pouches, and rigid containers. Proper packaging procedures are outlined including preparation, closure methods, labeling, and storage standards. Maintaining sterility through careful handling and following event-related concepts is emphasized.
The document summarizes the requirements for an IV room project based on USP <797> standards. It discusses the primary engineering controls including laminar airflow workbenches and biological safety cabinets. It also outlines the architecture of buffer and ante areas, recommended devices for compounding, cleaning and disinfecting frequency guidelines, personnel hygiene and garbing procedures, packaging and labeling standards, and staff responsibilities. The IV room is designed to meet most USP requirements, with some exceptions like not fully separating the buffer and ante areas into different rooms.
Pharmacy CSP Preparation: Personnel Garbing and GlovingJerry Fahrni
Personnel garbing and gloving procedures are outlined to minimize contamination when compounding sterile preparations. Individuals are excluded if shedding particles at high rates from infections or wounds. Garbing begins with removing jewelry and outerwear then donning shoe covers, hair/beard covers, and a mask in the ante-area. Hands and forearms are washed to the elbows for 30 seconds followed by drying and donning a gown. In the buffer area, hands are scrubbed with alcohol-based sanitizer and allowed to dry before donning sterile gloves. Gloves are disinfected throughout compounding and replaced if breached. PPE is replaced or redonned as needed to maintain sterility when reentering the compound
The document discusses the importance and functions of the Central Sterile Supply Department (CSSD) in hospitals. It outlines the history and objectives of CSSDs, which aim to provide sterilized medical supplies from a central location. The optimal design principles are described, including workflow zones and layout. Key areas like cleaning, sterilization, storage and distribution are explained. Finally, the document provides an inventory of recommended equipment for setting up an ideal CSSD.
Haldor's flagship solution, the ORLocate™ system, tracks, manages and analyses usage patterns of surgical instruments using radio frequency identification (RFID) technology. This highly modular, reliable, scalable and accurate system blends seamlessly with the workflow in the sterile processing department and during the surgical procedures. It also provides the capabilities to interact with the hospital’s real-time location system as well as hospital information systems and technologies. The ORLocate™ system is FDA 510(k) cleared and CE certified.
This document summarizes the development of a cost-effective test tube holder for a cell sorting instrument. A team created a custom device to hold test tubes of varying sizes and included a magnetic stirring plate to mix fluids during sorting, improving product collection and cell viability. Their design costs significantly less than the manufacturer's device and provides extra functionality. They successfully tested cell sorting and mixing with the new holder.
Three 3D printed models of a custom test tube holder were created for the BD FACS ARIA III cell sorting machine. Each model can hold different combinations of test tube sizes. Testing showed that a model holding two 50mL test tubes sorted cells with 88.77% accuracy, sorting different cell types into their respective test tubes. A magnetic stirring plate was attached to help mix fluids inside the test tubes. Results demonstrated that the stirring plate successfully mixed fluids together.
An analyst for NYP's Capital Asset Planning & Development Group, I compiled this presentation to share with senior leadership. The research is based on a a study done by the Institute for the Future while the conclusions of Hospital implications were my own. I hope you find this presentation useful as well as inspirational. Thanks for watching.
A Alusolda oferece soluções para solda e corte há mais de 30 anos, locando equipamentos, vendendo acessórios e prestando serviços de manutenção qualificados. A empresa busca a satisfação dos clientes através da melhoria contínua e conta com profissionais treinados para atender demandas relacionadas à manutenção e utilização de equipamentos de solda.
" Tudo sobre os Lions " é uma apresentação em Powerpoint, que relata os marcos importantes da histório dos Lions Clubes. Marcos que todos os Lions devem conhecer.
O documento fornece orientações sobre como redigir um projeto de pesquisa, abordando questões como escolha do tema, revisão de literatura, objetivos, metodologia e estrutura do projeto. É destacada a importância do projeto para planejar, organizar e subsidiar a pesquisa, além de permitir a avaliação dos orientadores e da banca examinadora. São explicados os componentes essenciais de um projeto como introdução, objetivos, justificativa, revisão bibliográfica, metodologia, cronograma e referências.
O documento descreve o sistema genital masculino, incluindo os testículos, que produzem espermatozoides e testosterona, e os ductos que transportam os espermatozoides.
SAVASSI, LCM; PEREIRA, RPA. Federal University of Ouro Preto report on rural family medicine teaching: Rural internship and family medicine discipline. Lecture at World Wonca Congress
2015 oficina mudança de comportamento 13o cbmfc natalLeonardo Savassi
SENS, GR; SAVASSI, LCM. OFICINA Mudança de Comportamento (Parte 1). In: 13o Congresso Brasileiro de Medicina de Família e Comunidade. Natal-RN: SBMFC, 2015. [palestra] [online] [disponível em https://sites.google.com/site/leosavassi/] [acesso em ##/##/20##]
O documento discute a importância da humanização na formação do fisioterapeuta. Ele descreve como a humanização é incorporada no currículo da FTC/Ssa através de três eixos: 1) atuação docente focada no mundo real, 2) práticas curriculares como visitas técnicas e estágios, 3) avaliação reflexiva dos estudantes. Vários programas de extensão são citados como exemplos de como a humanização é aplicada na prática.
O documento discute a atenção domiciliar na Atenção Básica à Saúde, definindo suas modalidades (AD1, AD2, AD3) de acordo com a legislação e propondo ferramentas para classificação de risco, vulnerabilidade e necessidade dos pacientes.
Como publicar apresentações no SlideShareMariaQuiteria
O documento explica como publicar apresentações de slides no SlideShare e incorporá-las em blogs. Resume os passos: 1) Criar conta no SlideShare; 2) Enviar apresentação e fornecer metadados; 3) Publicá-la após conversão; 4) Verificar e-mail para liberar recursos.
This document provides guidelines on safety regulations for school science labs. It discusses aseptic techniques like sterilizing surfaces and wearing gloves to prevent contamination. It also describes how to properly use autoclaves to sterilize equipment and autoclave tape to indicate sterilization. Biological safety cabinets and laminar flow cabinets are compared. Proper centrifuge use and sample labeling are outlined. Procedures for dealing with spills, accidents, and injuries involving microorganisms, chemicals and electricity are provided.
This document provides information on Jotun Thinner No. 17, including identification, hazards, composition, first aid measures, fire-fighting measures, accidental release measures, handling and storage, exposure controls, and personal protection. It identifies the product as a solvent-borne thinner and lists its manufacturer as Jotun Paints, Inc. It warns that the product is flammable and may cause eye, skin, and respiratory irritation as well as target organ damage and cancer.
Chlorinated paraffin (CP) is a complicated chemical that has a wide range of uses in a variety of industries. It is frequently used in rubber, paints, adhesives, caulks, sealants, and plastics as a secondary plasticizer and flame retardant. Another application is as a lubricant or coolant in the cutting or shaping of metal.
Elchemy is trusted chlorinated paraffin supplier, and exporter, offering high-quality and innovative chemical products to a wide range of industries. For more details, visit our website now!
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1) Sterilization and disinfection have a long history dating back to ancient Greek and Roman times when practices like boiling water and hand washing were used.
2) In the 1800s, figures like Semmelweiss, Nightingale, and Lister advanced practices of sterilization and antisepsis in hospitals and operating rooms which reduced infection rates and mortality.
3) Modern sterilization practices in operating rooms focus on preventing surgical site infections through strict protocols for cleaning, disinfection, air filtration, sterile techniques, and care of equipment, personnel, and facilities.
The Central Sterile Supply Department (CSSD) is responsible for receiving, processing, sterilizing, storing and distributing sterile surgical instruments and supplies. The document outlines the various processes involved in CSSD such as decontamination, packaging, sterilization and storage. It provides guidelines on CSSD work flow, cleaning and sterilization methods, packaging, load monitoring, and sterile storage area requirements to ensure sterility of supplies.
lecture 10.Procedures for Handling Hazardous SpillsRaghda alomari
The document provides guidance on handling hazardous chemical and biological spills in laboratories. It outlines factors to consider in spill risk assessment and describes general spill cleanup procedures. For chemical spills, it recommends containing the spill, neutralizing acids and bases, cleaning with water, and reporting the incident. For biological spills, it advises treating all spills as potentially infectious and following cleanup procedures using appropriate personal protective equipment and disinfectants. Proper spill kits and training are important to safely manage spills of hazardous materials.
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UCM SOP
1. Environmental Health and Safety
Standard Operating Procedure
BD FACSARIA III
Purpose
Test cell viability and determine whether the prototype cell collector is able to collect cells
Physical & Chemical Properties/Definition of Chemical Group
CAS#: not applicable
Class: not applicable
Molecular Formula: not applicable
Form (physical state): not applicable
Color: not applicable
Boiling point: not applicable
Potential Hazards/Toxicity
Disposing of cells into the appropriate biohazard container. As well as the potential to endanger ourselves
due to deflection plates that produce electricity.
Personal Protective Equipment (PPE)
Respiratory Protection not applicable
Respirators should be used only
under any of the following circumstances:
• As a last line of defense (i.e., after engineering and administrative controls have been
exhausted).
• When Permissible Exposure Limit (PEL) has exceeded or when there is a possibility that PEL will
be exceeded.
• Regulations require the use of a respirator.
• An employer requires the use of a respirator.
• There is potential for harmful exposure due to an atmospheric contaminant (in the absence of
PEL)
• As PPE in the event of a chemical spill clean-up process
Lab personnel intending to use/wear a respirator mask must be trained and fit-tested by EH&S. This is a
regulatory requirement.
Hand Protection
Latex gloves
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2. Environmental Health and Safety
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are
compatible with cell collection
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
Safety glasses or chemical splash goggles. Goggles are preferred.
Skin and Body Protection
Lab personnel working with the chemical need to wear full-length pants or its equivalent, closed-toe
footwear with no skin being exposed, and a lab coat.
Hygiene Measures
Wash hands after working with the substance.
Engineering Controls
The tube which contains the cells will be placed in a compartment where the tube is held inside the
equipment (ARIA) which has the ability to close therefore there will not be any interaction between the
cells and the person’s conducting the experiment.
First Aid Procedures
If inhaled
Evacuate the victim to a safe area as soon as possible. Loosen tight clothing such as a collar, tie, belt or
waistband. If breathing is difficult, administer oxygen. If the victim is not breathing, perform mouth-to-
mouth resuscitation. WARNING: It may be hazardous to the person providing aid to give mouth-to-mouth
resuscitation when the inhaled material is toxic, infectious or corrosive. Seek immediate medical attention.
In case of skin contact
In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing
contaminated clothing and shoes. Cold water may be used. Wash clothing before reuse. Thoroughly
clean shoes before reuse. Get medical attention immediately.
In case of eye contact
Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water
for at least 15 minutes. Cold water may be used. Get medical attention.
If swallowed not applicable
Special Handling and Storage Requirements
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In order to insure cell viability, cells are placed inside a refrigerator to keep cool and removed when
needed.
Spill and Accident Procedure
Chemical Spill Dial 9-911 and 228-7864
Spill – Assess the extent of danger. Help contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material.
Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic
bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 and EH&S at 228-7864 for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower
for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S at 228-7864 immediately.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water from the
emergency eyewash station for 15 minutes by forcibly holding the eye open. Seek medical attention.
Notify supervisor and EH&S at 228-7864 immediately.
Medical Emergency Dial 9-911 or 228-7864
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 9-911 Note: All serious
injuries must be reported to EH&S at 228-7864 within 8 hours.
Non-Life Threatening Emergency – Go to the Olivewood Meadows Occupational Health 374 Olive
during regular business hours. All other times report to Mercy Medical Center 315 Mercy Ave. Note: All
serious injuries must be reported to EH&S at 228-7864 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected
area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the
affected area for 15 minutes using an eyewash station. Go to the Olivewood Meadows Occupational
Health 374 Olive Ave. during regular business hours. All other times report to Mercy Medical Center 315
Mercy Ave. Note: All needle stick/puncture exposures must be reported to EH&S at 228-7864 within 8
hours.
Decontamination/Waste Disposal Procedure
Cells will be disposed of in the appropriate biohazard waste container as instructed by the Faculty
Supervisor.
General hazardous waste disposal guidelines:
Label Waste
• Affix an on-line hazardous waste tag on all waste containers using the Online Tag Program
https://ehs.ucop.edu/waste as soon as the first drop of waste is added to the container
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Store Waste
• Store hazardous waste in closed containers, in secondary containment and in a designated
location
• Double-bag dry waste using transparent bags
• Waste must be under the control of the person generating & disposing of it
Dispose of Waste
• Dispose of regularly generated chemical waste within 90 days
• Call EH&S at 228-7864 for questions
• Empty Containers
o Dispose as hazardous waste if it once held extremely hazardous waste (irrespective of
the container size) A list can be found at
http://ehs.ucla.edu/Pub/ExtremelyHazardousWaste.pdf
Prepare for transport to pick-up location
Check on-line waste tag
Use secondary containment
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://ehs.ucmerced.edu/material-safety-data-sheets.
Protocol/Procedure
Start Up
Note. If you are the first user of the day, steps 1-9 will already be completed for you. If you need to
change nozzles or experience a major blockage, start from step 1.
1. Empty waste (A) down the lab sink if full. Decant 1L 70% Ethanol into the empty waste container.
2. Refill sheath tank (B) with 1x PBS to the top 'fill line' (Note. Use the autoclaved 1x PBS prepared
especially for this instrument. DO NOT use the NERL Diluent Sheath Fluid that is used for the
LSR2). Ensure air and fluid lines are connected to tank.
3. Switch on the computer and log in to Windows using the Admin account and password “BDIS”.
Wait for Windows to load until the network icon appears in the lower right system taskbar. Turn
on the compressed air line (orange valve on the wall). Then turn on the instrument (C), AMO (Aria
III only) and circulating water bath (4˚C, Aria III only).
4. Log in to windows (password BDIS). Open the program BD FACSDiva, log in using your
username and password. Wait for the cytometer to connect.
5. From the dropdown menu, select Cytometer>Fluidics startup, follow the prompts.
6. Note the nozzle size needed. Select the appropriate nozzle, and follow the steps in Installing the
Nozzle to ensure the configuration is correct.
7. Bleed sheath line filter (D) of air. Turn on the stream (red X on the stream window) (E). Allow 3-5
minutes for the stream to settle. Adjust the Amplitude so that the break-off point (S) is on screen
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(Drop 1 should be ~100-300), and the Gap is stable: 70um ~6-10; 100um ~10-18 the frequency
node should be appropriate at the previously set frequency. If you want to inspect/change the
frequency node. A good check for stream stability is that side streams (F) are clean and stable.
8. Run CST
9. Turn the Sweet Spot (G) on
10. Set the drop delay
11. Set the side stream (F) trajectory
12. Run 10% bleach for 2 minutes, followed by DI H20 for 1 minute. Once you have finished your
sort, you should check the calendar booking system to see if you are the last user of the day. If
there is someone after you, complete the Final Clean. If you are the last user of the day, complete
the Shut Down procedure.
Final Clean
1. Run 10% bleach for 5 minutes
2. Run DI H20 for 5 minutes
3. Log out of your username in BD FACSDiva
Shut Down
1. Run 10% bleach for 5 minutes
2. Run DI H20 for 5 minutes
3. Turn the stream off (green tick on the Stream Window) (E)
4. Remove the nozzle and place it into its allocated 5ml tube containing fresh DI H20. Install the
closed loop nozzle. With ~4ml of DI H20 on the sample loading station (T),
selectCytometer>Cleaning Modes>Flow Cell Clean from the drop down menu. Repeat.
5. Export your data.
6. Quit BD FACSDiva, turn off the cytometer (C), computer and AMO. De-pressurise the sheath
tank (B).
Bulk sort
The collection apparatus will have a bulk sort into desired collection tube type of 50ml and two 5ml
collection tubes that can be housed simultaneously.
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1. The amount of populations that will be collected will be noted and the collection housing will be
chosen accordingly.
2. The housing will be filled with blank dummy collection tubes and slide into place.
3. Press voltage (O) and test sort (P). Open the waste drawer (Q), and open the front of the sort
chamber.
4. Move the side stream charge sliders (R) out to a point where the side stream is targeted to the
bottom of its corresponding collection tube (verify this by looking down through the sort chamber
to the collection tubes below). Only set up those side streams you are planning to use.
5. UCM net ID will be used as the Diva username, a password must be made
6. If the Closed Loop Nozzle is in place, store it in the nozzle holder (H) to the right of the sort
chamber.
7. The values on the right side are the live values of the Gap/Drop 1.
8. The up/down arrows of the frequency field will adjust the frequency by 0.1 MHz
9. One node of potentially several
10. The bead lot is displayed on the bead vial
11. When the optical filter is applied, you will not be able to see the side stream. Turn the filter on and
off again to approximate the position of the side stream within it.
12. If you cannot attain 100, try adjusting the Accudrop laser.
13. For most 1 or 2-way applications, this will be purity. For most 3 or 4-way applications, this will be
4-way.
14. The goal here is to accurately find the point where the most beads (highest left side stream
number) are deflected. This implies that the drop delay reflects the time is takes for a segment of
stream to move from the laser interrogation point to the droplet break-off (S).
NOTE
Any deviation from this SOP requires approval from PI.
Documentation of Training
• Prior to conducting any work with BD FACSARIA III, designated personnel must provide training to
his/her laboratory personnel specific to the hazards involved in working with this substance, work
area decontamination, and emergency procedures.
• The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a
copy of the SDS provided by the manufacturer.
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• The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate
laboratory safety training or refresher training within the last one year.
I have read and understand the content, requirements, and responsibilities of this SOP:
Name Signature Date
.
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