rDNA technology involves deliberately manipulating the genetic makeup of cells or organisms in a controlled way. Its major goals are to form gene libraries, isolate and amplify genes of interest, and alter genetic makeup and gene expression patterns. The key steps are isolating DNA from cells, fragmenting the DNA with restriction enzymes, amplifying the gene of interest with PCR, and inserting the recombinant DNA into a host with enzymes.

2. WHAT IS rDNA TECHNOLOGY?
• Is a deliberate and directional manipulation of the genetic make up of a
cell or organism in a pre determined way for producing a genetically
altered cell or organism.
• It is the manipulation of genes in a desired manner under controlled
conditions.
• Also known as gene splicing, gene manipulation or genetic modification.

3. MAJOR GOALS OF RDNA TECHNOLOGY:
 Formation of gene library
 Identification, isolation and amplification of desired genes
 Study of the expression of specific genes.
 Study of the regulation of expression of selected genes
 Alteration and modifications in the genetic make up of cell or organisms.
 Alteration in the pattern of gene expression.

5. STEPS INVOVLED IN RDNA CONSTRUCTION
1. Isolation of DNA:Enzymes such as cellulase (plant cells), lysozyme
(bacteria) and chitinase (fungi) are used to isolate pure DNA from the
cells.
2. Fragmentation of DNA:The isolated and purified DNA is treated with
restriction endonucleases which cut the DNA into fragments.
3. Amplification of Gene of Interest: Polymerase chain reaction (PCR) is
a process to amplify the gene once the proper gene of interest has been
cut using the restriction enzymes. Through this process, multiple
copies of the gene of interest can be produced.

6. 4. Insertion of recombinant DNA into the host: The desired dna is inserted
into the host dna with the help of various enzymes

8. QUESTIONS:
Q1. What is rDNA technology? What are the major goals of this
technology?
OR
Define rDNA technology and list the major goals.
Q2. List out the major steps involved in the rDNA construction.