Antibiotics are organic compounds produced by microorganisms that inhibit or kill other microorganisms at low concentrations. They are commonly produced by bacteria and fungi. Penicillin was the first natural antibiotic discovered from Penicillium molds by Alexander Fleming. It contains a beta-lactam ring structure and includes natural and semi-synthetic types. Citric acid is a weak organic acid found in citrus fruits that is produced commercially through fermentation using the fungus Aspergillus niger. It has various industrial uses and millions of tons are produced annually mainly through submerged fermentation.
PREPARATION OF BACTERIAL VACCINES:
Steps involved in killed bacterial vaccine preparation:
1. Selection of an antigen:
The exact strain or strains to be incorporated for preparation of bacterial vaccine.
Eg. Cholera vaccine: smooth strains of the two serological types Inaba and Ogawa
TABC vaccine: O and H antigens in S. typhi and S. paratyphi microorganisms and these organisms also contains Vi antigen.
Each strain is carefully checked for freedom from variation and absence of contaminating organisms.
Use of microbes in industry. Production of enzymes-General consideration-Amyl...Steffi Thomas
Industrial uses of microbes, properties of useful industrial microbes, various industrial products, production of enzymes-general consideration-amylase, catalase, peroxidase, lipase, protease, penicillinase, procedure for culturing bacteria and inoculum preparation, submerged fermentation and solid state fermentation, uses of different enzymes
BOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT MICROBIAL BIO TRANSFORMATION WITH BIOCHEMICAL REACTIONS
UNIT 6 Fermentation technology, Fermenters, Study of Media, types of fermenta...Shyam Bass
UNIT-6 6th Sem B.Pharma Pharmaceutical Biotechnology-
Following slides include-
Fermentation technology and biotechnological products :
Fermentation methods and general requirements
Study of media
Equipment
Sterilization methods
Aeration process
Stirring
large scale production fermenter design and its various controls
BY- SHYAM BASS
PREPARATION OF BACTERIAL VACCINES:
Steps involved in killed bacterial vaccine preparation:
1. Selection of an antigen:
The exact strain or strains to be incorporated for preparation of bacterial vaccine.
Eg. Cholera vaccine: smooth strains of the two serological types Inaba and Ogawa
TABC vaccine: O and H antigens in S. typhi and S. paratyphi microorganisms and these organisms also contains Vi antigen.
Each strain is carefully checked for freedom from variation and absence of contaminating organisms.
Use of microbes in industry. Production of enzymes-General consideration-Amyl...Steffi Thomas
Industrial uses of microbes, properties of useful industrial microbes, various industrial products, production of enzymes-general consideration-amylase, catalase, peroxidase, lipase, protease, penicillinase, procedure for culturing bacteria and inoculum preparation, submerged fermentation and solid state fermentation, uses of different enzymes
BOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT MICROBIAL BIO TRANSFORMATION WITH BIOCHEMICAL REACTIONS
UNIT 6 Fermentation technology, Fermenters, Study of Media, types of fermenta...Shyam Bass
UNIT-6 6th Sem B.Pharma Pharmaceutical Biotechnology-
Following slides include-
Fermentation technology and biotechnological products :
Fermentation methods and general requirements
Study of media
Equipment
Sterilization methods
Aeration process
Stirring
large scale production fermenter design and its various controls
BY- SHYAM BASS
VIRAL VACCINES
Since viruses are intracellular parasites they will grow only within other living cells.
Methods of viral vaccine production:
Cultivation of virus using free living animals
Fertile eggs
Tissue cultures
General method of the preparation of Bacterial Infections, Toxoids, Viral vaccine, Antitoxins, Serum-immune blood derivatives and other products relative to immunity.
genetic engineering, principles, b pharma 6th sem, biotechnology
What is a gene ?
Definition
History
Process
Molecular tools of genetic engineering
Restriction enzymes
History of restriction enzyme
Mechanism of action
Types of restriction enzymes
Application of restriction enzymes
Blunt ends
Sticky ends
transgenic
cisgenic.
knockout organism.
Host organism vector
TRANSGENIC PLANTS
DOLLY THE SHIP
TRANSGENIC ANIMALS
VIRAL VACCINES
Since viruses are intracellular parasites they will grow only within other living cells.
Methods of viral vaccine production:
Cultivation of virus using free living animals
Fertile eggs
Tissue cultures
General method of the preparation of Bacterial Infections, Toxoids, Viral vaccine, Antitoxins, Serum-immune blood derivatives and other products relative to immunity.
genetic engineering, principles, b pharma 6th sem, biotechnology
What is a gene ?
Definition
History
Process
Molecular tools of genetic engineering
Restriction enzymes
History of restriction enzyme
Mechanism of action
Types of restriction enzymes
Application of restriction enzymes
Blunt ends
Sticky ends
transgenic
cisgenic.
knockout organism.
Host organism vector
TRANSGENIC PLANTS
DOLLY THE SHIP
TRANSGENIC ANIMALS
BOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT PRODUCTION OF CITRIC ACID , PENICILLIN, GLUTAMIC ACID , GRISIOFULVIN , VITAMIN B 12
production of penicillin b pharma 6th sem by piyush lodhiDrx Piyush Lodhi
project b pharma 6th sem.
Penicillium mold naturally produces the antibiotic penicillin. ... Scientists learned to grow Penicillium mold in deep fermentation tanks by adding a kind of sugar and other ingredients. This process increased the growth of Penicillium.
It is about information about well known pharmaceutical industry in Pune , which also includes production of penicillin and Streptomycin which is helpful for biotechnology students
Fermentation of Penicillin Antibiotic
Penicillin is an antibiotic produced by microorganisms. These antibiotics inhibit the growth and development of another micro-organism. Generally, the penicillin antibiotic is produced by some actinomycetes and some filamentous fungi. The antibiotics produced by these micro-organisms can be used medicine field, veterinary as well as agricultural field. Penicillin antibiotic was the first antibiotic used in large amount during world war second for treatment of soldiers. Penicillin is a antibiotic used against Gram positive bacteria as well as high dosage can be used against Gram negative bacteria. Penicillin is not harmful to plants, animals or human beings except in some cases of allergies
A broad module on industrial microbiology is summarized with pictures .It includes the production of vitamins,vaccine ,alcohol,vinegar,steroids,amino acids ,antibiotics .it also includes the general idea on history ,media,equipment,fermentation,procedure ,uses of industrial microbiology .The production of wine,beer and vinegar are mine core interest .Hope may help ....Thank you .
Fed-Batch fermentation for the Production of penicillin G from Penicillium Ch...Yassos Osman
Penicillium Chrysogenium is a very high yielding strain, this is why it is widely used in the production of Penicillin G (Benzylpenicillin). This is achieved by introducing spores of strain to appropriate environment so that spores can germinate and form mycelium which is the vegetative part of the mold, then microorganism is allowed to grow and reproduce, until stationary phase, it is then starting to produce penicillin.
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
2. What is anantibiotic?
The term antibiotic is refers to an organic compound that is
produced by one microorganism that, at great dilutions,
inhibits the growth of or kills another or even group of other
harmful microorganisms.
Antibiotics are available in various forms.
For example,
As ointment or cream to treat bacterial infection on the
surface.
As tablet/capsules/injection to treat internal infection.
3. Antibiotics are produced primarily by bacteria, Streptomycetes,
Nocardia and fungi. However, antibiotics produced by
Streptomycetes spp. Having greatest commercial applications.
On the basis of mode of action antibiotics can be divided as:
Antibiotics affecting cell wall
Antibiotics damaging cell membrane
Antibiotics interfering with protein synthesis
Antibiotics inhibiting nucleic acid synthesis
Antibiotics blocking cell metabolism
What is antibiotic
4. Penicillin
• The first discovered natural antibiotic was Penicillin which was
obtained from multicellular fungi, “Penicillium molds”.
• Penicillin is a group of compounds having 6-amino penicillinic
acid, contains ring like structure termed as a β-lactam ring.
• Penicillin are of two different types,
1. Natural Penicillin (directly harvested from the
penicillium mold).
2. Semi-synthetic Penicillin consists of the basic
Penicillin nucleus (6-APA), but with new side chain. It
includes Ampicillin, Methicillin, Penicillin V, etc.
5. Penicillin
• The first discovered natural antibiotic was Penicillin by Dr
Flemming; which was obtained from multicellular fungi,
“Penicillium molds”.
• Penicillin is a group of compounds having 6-amino penicillinic
acid, contains ring like structure termed as a β-lactam ring.
• Penicillin are of two different types,
1. Natural Penicillin (directly harvested from the
penicillium mold).
2. Semi-synthetic Penicillin consists of the basic
Penicillin nucleus (6-APA), but with new side chain. It
includes Ampicillin, Methicillin, Penicillin V, etc.
6. Production of Penicillin
Inoculum
• P. notatum & P. chrysogenum are two species used.
• P. chrysogenum is high yielding strain and therefore most widely
used as production strain.
• A starter culture transferred to an agar containing plate to allow
growth.
• After getting growth on solid media, one or two growth stages
should allowed in shaken flask cultures to create a suspension,
which can be transferred to seed tanks for further growth.
• Feed tank contain all the nutrients including Growth factors like
vitamins & amino acid as well as it may maintain temperature,
pH, agitation, aeration etc.
7. Media
Components Percent(%) Use
Lactose 3.5 to 6 C-sourse
Calcium Carbonate 1.0 buffer
Corn steep Liquor 3.5 N-source
Glucose 1.0 C-sourse)
Phenyl acetic acid 0.5 Precursor
Sodium hydrogen phosphate 0.4 catalyst
Edible oil 0.25
Bio parameters
• pH: near 6.5
• Temperature: 26°C to 28°C
• Aeration: a continuous stream of sterilized air is pumped into it.
• Agitation: achieved by turbine type impellers for constant agitation.
• Time period 5-6 days
Production of Penicillin
8. Recovery and purification
• Vacuum filter is used for separation of mycelium
from the broth on a rotary.
• The lowering of pᴴ is done by adding phosphoric
acid or sulphuric acid for conversion of penicillin to
the anionic form occurs at low pᴴ (2.0 to 2.5).
• For removal of pigment and other impurities from
solvent containing penicillin it is treated with active
charcoal.
• The product of penicillin is then crystallized into
sodium or potassium penicillin.
Production of Penicillin
9. Citric Acid
Citric acid is the most important commercial product,
which is found in almost all plant & animal tissues.
Citric acid is the most important organic acid produced in
tonnage and is extensively used in food and
pharmaceutical industries.
Citric acid is a weak organic acid found in citrus
fruits(lemon).
It is natural preservative and is also used to add an acidic
taste to food and soft drinks.
Million tonnes of citric acid are produced every year by
fermentation.
10. Inoculum
• The fungus Aspergillus niger is used.
• Mutant strain of Aspergillus niger is used for improved
production.
• It can tolerate high sugar concentration and low pH with
reduced synthesis of undesired byproducts.
Industrial fermentationprocess is carried out in three different ways as:
1. Surface fermentation
2. Submerged fermentation
3. Solid state fermentation
Production of Citric acid
11. Surface Fermentation
a. Solid Surface Fermentation
Carried out in some Asian countries like Japan using
A. niger.
Solid Substrate such as wheat bran or pulp from
sweet potato starch are used, as culture media.
Fermentation is done at pH is kept 4-5 at incubation
temp. of 280 C for 80-100 h.
Recovery and isolation is done using hot water .
Production of Citric acid
12. Surface Fermentation
b. Liquid Surface Fermentation
This is oldest method & carried out using liquid
nutrient such as beet carried out in aluminium trays
filled with nutrient media.
Inoculum in the form of spores sprayed.
Temperature spread 300 C
The pH of medium fall below 2 , as mycellium grows.
After 7-15 days , the mycellium is separated, pressed
mechanically and washed to get maximum amout of
citric acid.
Production of Citric acid
13. SUBMERGEDFERMENTATION:
• In this case, the strains are inoculated of about 15cm
depth in fermentation tank.
• The culture is enhanced by giving aeration using air
bubbles. And its allowed to grow for about 5 to 14
days at 27 to 33 degree Celsius.
• The citric acid produced in the fermentation tank and
it is purified.
Production of Citric acid
14. Solid statefermentation:
• It is simplest method for citric acid production.
• Solid state fermentation is also known as Koji
process, was first developed in Japan.
• Citric acid production reached a
maximum(88g/kg dry matter) when
fermentation as carried out with cassava
having initial moisture of 62% at 26degree
Celsius for 120 hours.
Production of Citric acid
15. Recovery
• The mycellium is separated by filtration of culture broth
and washing.
• Oxalic acid as unwanted byproduct is separated by
precipitation by adding lime.
• The liquid is transferred to recovery process
• Separation of citric acidis done at pH 7.2 and temp. 70-90
degree by precipitation.
• Further purification is done by dissolving citric acid in
sulfuric acid.
• Finally treated with activated charcoal, cation, anion
exchanger and crystallize
Production of Citric acid