The document discusses the production of several pharmaceutical products through microbial fermentation processes. It describes the fermentation of penicillin using Penicillium chrysogenum, the production of citric acid using Aspergillus niger, and the microbial synthesis of vitamin B12, glutamic acid, and other compounds. Key details provided include microbial strains used, fermentation media composition, culture methods, and process parameters.
BOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT PRODUCTION OF CITRIC ACID , PENICILLIN, GLUTAMIC ACID , GRISIOFULVIN , VITAMIN B 12
Fermentation of Penicillin Antibiotic
Penicillin is an antibiotic produced by microorganisms. These antibiotics inhibit the growth and development of another micro-organism. Generally, the penicillin antibiotic is produced by some actinomycetes and some filamentous fungi. The antibiotics produced by these micro-organisms can be used medicine field, veterinary as well as agricultural field. Penicillin antibiotic was the first antibiotic used in large amount during world war second for treatment of soldiers. Penicillin is a antibiotic used against Gram positive bacteria as well as high dosage can be used against Gram negative bacteria. Penicillin is not harmful to plants, animals or human beings except in some cases of allergies
Use of microbes in industry. Production of enzymes-General consideration-Amyl...Steffi Thomas
Industrial uses of microbes, properties of useful industrial microbes, various industrial products, production of enzymes-general consideration-amylase, catalase, peroxidase, lipase, protease, penicillinase, procedure for culturing bacteria and inoculum preparation, submerged fermentation and solid state fermentation, uses of different enzymes
BOTECHNOLOGY IS CHALLENGING SUBJECT TO TEACH AND UNDERSTAND ALSO .....THEIR INTERESTING PART IS TO LEARN ABOUT PRODUCTION OF CITRIC ACID , PENICILLIN, GLUTAMIC ACID , GRISIOFULVIN , VITAMIN B 12
Fermentation of Penicillin Antibiotic
Penicillin is an antibiotic produced by microorganisms. These antibiotics inhibit the growth and development of another micro-organism. Generally, the penicillin antibiotic is produced by some actinomycetes and some filamentous fungi. The antibiotics produced by these micro-organisms can be used medicine field, veterinary as well as agricultural field. Penicillin antibiotic was the first antibiotic used in large amount during world war second for treatment of soldiers. Penicillin is a antibiotic used against Gram positive bacteria as well as high dosage can be used against Gram negative bacteria. Penicillin is not harmful to plants, animals or human beings except in some cases of allergies
Use of microbes in industry. Production of enzymes-General consideration-Amyl...Steffi Thomas
Industrial uses of microbes, properties of useful industrial microbes, various industrial products, production of enzymes-general consideration-amylase, catalase, peroxidase, lipase, protease, penicillinase, procedure for culturing bacteria and inoculum preparation, submerged fermentation and solid state fermentation, uses of different enzymes
A broad module on industrial microbiology is summarized with pictures .It includes the production of vitamins,vaccine ,alcohol,vinegar,steroids,amino acids ,antibiotics .it also includes the general idea on history ,media,equipment,fermentation,procedure ,uses of industrial microbiology .The production of wine,beer and vinegar are mine core interest .Hope may help ....Thank you .
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Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
A broad module on industrial microbiology is summarized with pictures .It includes the production of vitamins,vaccine ,alcohol,vinegar,steroids,amino acids ,antibiotics .it also includes the general idea on history ,media,equipment,fermentation,procedure ,uses of industrial microbiology .The production of wine,beer and vinegar are mine core interest .Hope may help ....Thank you .
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Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
Follow us on: Pinterest
Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
The Gram stain is a fundamental technique in microbiology used to classify bacteria based on their cell wall structure. It provides a quick and simple method to distinguish between Gram-positive and Gram-negative bacteria, which have different susceptibilities to antibiotics
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Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
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Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
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Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
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2. Antibiotics, vitamins, enzymes and alcoholic products
is the main aim of the fermentation industry.
Most pharmaceuticals are produced by the process of
fermentation using specific microbial strains .
Antibiotics for therapy was discovered by scientists
Alexander Flaming in 1929 found that a world
Penicillin had an effect against Staphylococcus aureus.
Penicillin was the first antibiotic commercially
produced from the Penicillin Species by the process of
fermentation.
These antibiotics are mainly produced from fungi,
actinomycets and bacteria.
This substances have wide application in medical
,veterinary and agriculture practices.
These antibiotics produce by microbial fermentation
or chemical synthesis or combination of both.
3. Production of Penicillin began in US in 1941 by
surface culture fermentation of Penicillin
notatum.
Penicillin is produced from Penicillium
Chrysogenum by submerged culture techniques .
Penicillin is effective against gram positive
bacteria and also some large viruses and
rickettsia.
Natural penicillin penicillin V& G are effective
against several gram positive bacteria.
They inhibit the bacterial cell wall synthesis and
destroy the cell.
Penicillin is easily hydrolyzed by enzyme beta
lactamase.
4. The selected strain of Penicillin Chrysogenum is
maintained in the form of a master culture and
preserve by mixing the spores in sterilized soils.
For inoculum preparation spores from working solid
cultures are suspended in water .
These spores are added in a flask containing nutrient
solution and incubated for four to six days at 25° C .
The resulting force are used directly to inoculate
inoculum tank .
The inoculum tanks are incubated for 48 hours with
agitation and aeration to grow more mycelium.
The resulting inoculum is used for a production tank
or it is added to a second or even third stage
inoculum tank to produce more inoculum for large
scale fermentation .
5. The exact composition of penicillin production media
used in the industry for production of penicillin is
unknown.
A typical medium described by Jackson 1958 contains
fermentable carbohydrates such as corn steep liquor
solids 3.5%, lactose 3.5%, and glucose 1% ,
potential hydrogen phosphate 0.4%, calcium
carbonate 1% , edible oil 4% and Penicillin precursor .
The PH of this medium after sterilization is 5 to 6.
Inoculum Media are similar to production media
except the lactose and precursor are not added in the
Inoculum media.
These medium compositions may be slightly changed
to increase the yields and meet economic changes.
6. The media are placed in the fermentation vessel sterilize and
inoculated with a suspension of Penicillin chrysogenum.
The fermentation vessel is equipped with devices which allow
continuous addition of nutrients acid or bases to maintain the PH
7 to 7.4 and cooling coils to maintain the temperature 24 ° C .
Maximum antibiotics are produced with four to five days
The growth of mycelium (the mass of branched, tubular
filaments (hyphae) of fungi.)is occur in the 1st 20-30 hours.
For fermentation the mycelium grows rapidly by utilizing glucose
, lactic acid and organic nitrogen compounds.
fungal growth becomes very thick and heavy.
Initially PH may decreases with glucose utilization and
production of acids ammonia is liberated by Deamination of the
amino acid of corn steep liquor and ph becomes 7 to 7.5
Glucose and lactic acid are utilized first them lactose is available
for continuation of fermentation .
7.
8.
9. Citric acid eats natural constituent and common metabolite of
plant and animals it is the most versatile and widely used organic
acid in the field of food and pharmaceuticals
citric acid was isolated from lemons but today about 99 % of the
world’s citric acid produced from microbial fermentation .
Citric acid fermentation was first observed by Wehmer in 1893
in the fungus species of Penicillium glaucum.
Now Citric acid mainly produced by submerged fermentation
using aspargillus niger or Canada species from different sources
of carbohydrates .
Citric acid is mainly used in food industry because of its high
solubility pleasant taste .
It is commonly used as a flavoring agent in food and beverages
example jam , jellies , candies, deserts , soft drinks etc .
Citrix acid also act as a antioxidant and preservatives .
10. A large number of microorganisms are used
for production of citric acid including bacteria
example bacillus licheniformis,
corynebaterium sp.
Molds like Aspargilius niger Penicillium
luteum, Penicillium citrinum.Is like Candida
tropicals, Candida oleophila, Candida
Citroformans
Citric acid is primary metabolic product form
in tricarboxylic acid i.e kreb cycle
11. Mutant species of Aspargillus Niger is commonly
used for production of Citric acid for commercial use
Stock culture of species are maintained in the culture
tube in the form of lyophilized spores or storage in
refrigerator .
Spores are produced in glass bottle on solid
substrates up to 12 to 14 days incubation at 25 to 27
A nutrient medium containing 15 % sugar from
molasses is used in seed fermentation to induce
mycelium formation in the form of pellets .
The sports germinate at 30- 32 degrees Celsius
within 20 to 24 hours to form a pellets
These pellets are used as a inoculum for production
medium
12. Citric acid is produced by both surface as well as
submerged method
In Surface fermentation method Microorganisms can grow
as a layer or a film on a surface of nutrient medium which
may be liquid or solid in nature
liquid surface fermentation is the oldest method for
production of citric acid the more sophisticated and simple
method requires less effort in operation and installation
less energy cost and higher reproducibility .
The inoculum in the form of spores is spread over the
medium and fungus develops as a mycelial mat on the
surface of the medium
The trays are made of high purity of aluminum or special
grade steel .
Sterilized nutrient solutions automatically flow over a
distribution system onto the trays .
13. Microbial culture is inoculated in the incubation
chamber at 30 to 40 degree Celsius by blowing
dry spore or by spraying spore suspensions.
The fermentation chambers are provided with
an effective air circulation to control the
temperature 30 degree Celsius and humidity .
Within 24 hours after inoculation germinating
spores form a thin cover of mycelium on the
surface of medium
The fermentation is stopped after 7 to 14 days
14.
15. Vitamin B12 is water soluble vitamin commonly known as
Cobalamin.
It is an important dietary component for normal growth in
human being and animal Ricke E.L and Smith L in 1948
isolated small amount of active material from liver and
crystallized it and determine Vitamin B12 which was active in
the treatment of pernicious anemia.
Vitamin B12 is one of the largest and most complex
molecules
The main part in the structure of B12 is porphyrin ring
containing cobalt as a central element.
The cynide (CN)group, hydroxyl group(-OH) or nitride
group (-NO2) attached to the cobalt is called
CynoCobalamine, hydroxocobalamine and
Nitritocobalamine respectively .
Vitamin B12 is produced by bacteria and pseudomonas
denitrificans, bacillus subtilis, bacillus magaterium and
actinomycetes Nocardia species, streptomyces olivaceus ,
streptomyces griseus .
16. Pure culture of streptomyces olivaceus is
inoculated in inoculum medium contained in
Erlenmeyer flasks.
Bennett's broth [ east extract 1% , Beef extract 1
%, Glucose 1 % and enzymatic hydrolysate of
casein 25% ] is employed for development of
Inoculum.
Flash are kept on mechanical shaker during
incubation for the aeration
These flask cultures are used to inoculate the
large amount of inoculums media the required
amount of inoculums ( 5% of volume of
production medium) is prepared by successive
transfers.
17. The culture of streptomyces olivaceus is grown with aeration at
20 degrees Celsius in nutritionally reached crude medium
distiller soluble 4% dextrous 1% and calcium carbonate 0.5% are
present in typical production medium used in production of
vitamin B12
cobalt chloride( COCL2. 6H2O at approximately 2 -10 ppm is
added on this medium as a precursor
The p H of the medium is adjusted to 7 to 7.5 before
sterilization and may or may not be controlled during
fermentation .
In the 1st 24 hours the PH of fermentation is reduced due to
rapid sugar consumption and again ph increases after 48 to 96
hours due to lysis of mycelium.
A correct rate of aeration and speed of agitation are required for
proper growth of microbial strains
The optimum rate of aeration is about 0.5 volume air /volume
medium /minute
Soya bean oil corn oil etc are used as a antifoaming agent to
suppress the foam formation
18.
19. Glutamic acid was first amino acid to be
produced by microorganisms cynebacterium
glutamicum
Glutamic acid is widely used in the
production of monosodium glutamate which
is commonly known as a seasoning salt
Monosodium glutamate is flavor enhancing
agent and it is commonly used in convenient
food stuffs
20. L- glutamic acid can be synthesized from
wide species of bacteria actinomycets and
fungi
In addition of corynebacterium glutamicum
the other different strains are used for the
production of acid such as E- coli, bacillus
subtilis, cephalosporium , bacillus
megatherium etc.
21. A suitable strain of Corynebacterium
glutamicum from stock culture is used for
Inoculum development
This srain is inoculated in sterilized medium
and incubated at 35 degree Celsius for 16
hours
Sufficient inoculum 6% can be developed and
then added in a final production fermenter
22. The production medium consists of carbon sources
nitrogen sources minerals and vitamins one of production
medium for glutamic acid contains glucose k2HP of four
KH2PO4 , KH2PO4 , MnSO4.7H2O, urea and biotin .
Carbon sources Such as glucose , sucrose , fructose ,
maltose , sugarcane molasses are commonly used in
fermentation.
Since Mueller says he had high biotin content penicillin or
fatty acid derivatives must be added to the fermentation
Glutamic acid is intracellular components hence
production and excretion is depend upon cell permeability
Permeability of acid producing bacteria can be increased
by growth under biotin limiting condition , addition of
penicillin and saturated fatty acids and use of oleic acid
and glycerol auxotroph.
The concentration of ammonia Is very important for
converting carbon sources of glutamic acid biotin is an
important growth factor an essential coenzyme in fatty
acid synthesis .