Alexander Fleming accidentally discovered penicillin in 1928 when he found that the Penicillium fungus prevented bacterial growth. In the 1940s, Howard Florey and Ernst Chain purified penicillin into a powdered form. Penicillin is biosynthesized by Penicillium fungi through a three step process and derivatives were later developed with altered R groups to deal with bacterial resistance. Modern industrial production of penicillins uses high yielding Penicillium chrysogenum strains in a fed-batch fermentation process under controlled conditions to maximize antibiotic yield.
streptomycin production, uses, disadvantages , medium, inoculum preparation, commercial production, harvest and recovery process, biosynthetic pathway from glucose to streptomycin, flow sheet of streptomycin production by submerged culture method, chemical structure of streptomycin,
which functional unit have antibiotic activity?
Industrial production of penicillin.ppt523JoyceAngel
Industrial productioon of penicillin.
penicillin is a group of antibiotic obtained from fungi mold Penicillium notatum (in begining) ,Penicillium chrysogenum (used in present days due to high yield) and P. rubens. Most penicillins in clinical use are synthesised by P. chrysogenum .
First discovered Antibiotic.
Discovered by Alexander Fleming in 1928.
Penicillin is a group of antibiotics which includes Penicillin G, Penicillin V, Amoxillin, Ampicillin, Methicillin, Oxacillin, Dicloxallin, Carbenicillin, Propicillin and Benzathine penicillin.
Narrow spectrum antibiotic
Cell wall inhibitor – Inhibits peptidoglycan synthesis.
More effective against Gram positive bacteria.
5 steps in penicillin production
1.Selection of microorganism
2.Selection of raw materials
3. Preparation of inoculum
4. Fermentation process
5. Product recovery
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
streptomycin production, uses, disadvantages , medium, inoculum preparation, commercial production, harvest and recovery process, biosynthetic pathway from glucose to streptomycin, flow sheet of streptomycin production by submerged culture method, chemical structure of streptomycin,
which functional unit have antibiotic activity?
Industrial production of penicillin.ppt523JoyceAngel
Industrial productioon of penicillin.
penicillin is a group of antibiotic obtained from fungi mold Penicillium notatum (in begining) ,Penicillium chrysogenum (used in present days due to high yield) and P. rubens. Most penicillins in clinical use are synthesised by P. chrysogenum .
First discovered Antibiotic.
Discovered by Alexander Fleming in 1928.
Penicillin is a group of antibiotics which includes Penicillin G, Penicillin V, Amoxillin, Ampicillin, Methicillin, Oxacillin, Dicloxallin, Carbenicillin, Propicillin and Benzathine penicillin.
Narrow spectrum antibiotic
Cell wall inhibitor – Inhibits peptidoglycan synthesis.
More effective against Gram positive bacteria.
5 steps in penicillin production
1.Selection of microorganism
2.Selection of raw materials
3. Preparation of inoculum
4. Fermentation process
5. Product recovery
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
Penicillin has been one of the best Discoveries of Medical Science which literally changed the face of Medical Science. Life before Penicillin & Life after penicillin has shown tremendous difference in Life Span of Humans as well as other mammals .
Alexander Fleming showed the world THE POTENCY of ANTIBIOTICS ....
Vitamin B12 biosynthesis is restricted to microorganisms. Most of the steps in the
biosynthesis of vitamin B12 have been characterized in Pseudomonas denitrificans, Salmonella
typhimurium and Propionibacterium freudenreichii. Some authors have reported about the
requirement of more than 30 genes for the entire de novo biosynthesis of cobalamin, which
amounts to about 1 % of a typical bacterial genome. Two different biosynthetic routes for
vitamin B12 exist in nature:
• aerobic, or more precisely an oxygen-dependent pathway that is found in organisms like P.
denitrificans, and
• anaerobic, oxygen-independent pathway investigated in organisms like P. shermanii,
Salmonella typhimurium and Bacillus megaterium.
Extraction, Purification and Production of Enzymes (Biotechnology) Ajjay Kumar Gupta
Extraction, Purification and Production of Enzymes (Biotechnology) (Polystyrenes, Polypeptides, Polysaccharides, Proteins, Carbon, Propylene Oxide, Vinyl Chloride, Biosensors, Amino Acids, Antibiotics, Acrylamide, Organic Acids, Maltose Syrups, Hollow Fibres, Hollow Fibres, Enzyme Immunoassay (ELA), Enzyme Electrodes, Biocatalysts)
Industrial biotechnology is the practice of using cells to generate industrially useful products. An enzyme is a protein that catalyzes, or speeds up, a chemical reaction. Enzymes are the focal point of biotechnological processes, without them biotechnology as a subject would not exist. The main advantage of enzymes compared to most other catalysts is their stereo, region and chemo selectivity and specificity. Enzymes are responsible for many essential biochemical reactions in microorganisms, plants, animals, and human beings.
See more
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https://goo.gl/hMGIqd
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Contact us:
Niir Project Consultancy Services
106-E, Kamla Nagar, Opp. Spark Mall,
New Delhi-110007, India.
Email: npcs.ei@gmail.com , info@entrepreneurindia.co
Tel: +91-11-23843955, 23845654, 23845886, 8800733955
Mobile: +91-9811043595
Website: www.entrepreneurindia.co , www.niir.org
Tags
Enzymes in Biotechnology, Enzymes in Industrial Biotechnology, Enzymes and Biotechnology, Enzymes Biotechnology, Enzymes Used in Biotechnology, Biotechnology and Enzymes in Food Industry, Enzymes Used in Industry, Industrial Uses of Enzymes, Industrial Production of Enzymes, Production of Enzymes, Methods of Enzyme Production, Large Scale Production of Enzymes, Enzyme Production Methods, Enzyme Production, Production of Industrial Enzymes, Industrial Production Process of Enzymes, Enzyme Production and Purification, Enzyme Production Industry, Enzymes Manufacturing Plant, Manufacture and Formulators of Enzymes, Formulation of Enzymes, Enzymes Formulation, Purification and Formulation of Enzymes, Ethanol Fermentation, Bioaffinity Procedures, Phase Separation Method, Method and Formulation for Enzymes, Formulas for Enzymes, Formulae of Enzymes, Enzymic Production of Amino Acids, Method for Production of Enzymic of Amino Acids, Fruit Processing, Small Scale Fruit Processing, Enzyme Industry, Enzyme Industry in India, Enzyme Business, Profitable Biotechnology Business Ideas, Biotechnology Industry in India, Fruit Processing Industry, Fruits Processing Methods, Fruit Processing in India, Methods of Processing Fruits, Enzyme Inhibition, Methods of Purification of Enzymes, Enzyme Purification, Purification of Enzymes, Large-Scale Purification of Enzymes, Enzyme Extraction and Purification Process, Enzyme Purification Methods, Enzyme Biotechnology, Guide to Protein Purification, Cheese Production, Cheese Making Process, Cheese Manufacture, Cheese Production Process, Cheese Production Steps, Manufacture of Cheese, Manufacturing, Cheese, Cheese Making, Cheese Manufacturing
Use of microbes in industry. Production of enzymes-General consideration-Amyl...Steffi Thomas
Industrial uses of microbes, properties of useful industrial microbes, various industrial products, production of enzymes-general consideration-amylase, catalase, peroxidase, lipase, protease, penicillinase, procedure for culturing bacteria and inoculum preparation, submerged fermentation and solid state fermentation, uses of different enzymes
Production of tetracyclin and cephalosporinSamsuDeen12
Tetracyclin and cephalosporins are one of the major used antibiotics commonly all around the world. They are used to treat against microorganisms as a bactericidal, these eliminates those organisms in the host through various mechanism. These antibiotics are produced in a large scale using a bioreactors in many countries.
This PPT will provide the basic idea of Fermentation technology and it's use. The reference book is 'Pharmaceutical Biotechnology' by Giriraj Kulkarni.
Penicillin has been one of the best Discoveries of Medical Science which literally changed the face of Medical Science. Life before Penicillin & Life after penicillin has shown tremendous difference in Life Span of Humans as well as other mammals .
Alexander Fleming showed the world THE POTENCY of ANTIBIOTICS ....
Vitamin B12 biosynthesis is restricted to microorganisms. Most of the steps in the
biosynthesis of vitamin B12 have been characterized in Pseudomonas denitrificans, Salmonella
typhimurium and Propionibacterium freudenreichii. Some authors have reported about the
requirement of more than 30 genes for the entire de novo biosynthesis of cobalamin, which
amounts to about 1 % of a typical bacterial genome. Two different biosynthetic routes for
vitamin B12 exist in nature:
• aerobic, or more precisely an oxygen-dependent pathway that is found in organisms like P.
denitrificans, and
• anaerobic, oxygen-independent pathway investigated in organisms like P. shermanii,
Salmonella typhimurium and Bacillus megaterium.
Extraction, Purification and Production of Enzymes (Biotechnology) Ajjay Kumar Gupta
Extraction, Purification and Production of Enzymes (Biotechnology) (Polystyrenes, Polypeptides, Polysaccharides, Proteins, Carbon, Propylene Oxide, Vinyl Chloride, Biosensors, Amino Acids, Antibiotics, Acrylamide, Organic Acids, Maltose Syrups, Hollow Fibres, Hollow Fibres, Enzyme Immunoassay (ELA), Enzyme Electrodes, Biocatalysts)
Industrial biotechnology is the practice of using cells to generate industrially useful products. An enzyme is a protein that catalyzes, or speeds up, a chemical reaction. Enzymes are the focal point of biotechnological processes, without them biotechnology as a subject would not exist. The main advantage of enzymes compared to most other catalysts is their stereo, region and chemo selectivity and specificity. Enzymes are responsible for many essential biochemical reactions in microorganisms, plants, animals, and human beings.
See more
https://goo.gl/LBmTLd
https://goo.gl/hMGIqd
https://goo.gl/KjIzGj
Contact us:
Niir Project Consultancy Services
106-E, Kamla Nagar, Opp. Spark Mall,
New Delhi-110007, India.
Email: npcs.ei@gmail.com , info@entrepreneurindia.co
Tel: +91-11-23843955, 23845654, 23845886, 8800733955
Mobile: +91-9811043595
Website: www.entrepreneurindia.co , www.niir.org
Tags
Enzymes in Biotechnology, Enzymes in Industrial Biotechnology, Enzymes and Biotechnology, Enzymes Biotechnology, Enzymes Used in Biotechnology, Biotechnology and Enzymes in Food Industry, Enzymes Used in Industry, Industrial Uses of Enzymes, Industrial Production of Enzymes, Production of Enzymes, Methods of Enzyme Production, Large Scale Production of Enzymes, Enzyme Production Methods, Enzyme Production, Production of Industrial Enzymes, Industrial Production Process of Enzymes, Enzyme Production and Purification, Enzyme Production Industry, Enzymes Manufacturing Plant, Manufacture and Formulators of Enzymes, Formulation of Enzymes, Enzymes Formulation, Purification and Formulation of Enzymes, Ethanol Fermentation, Bioaffinity Procedures, Phase Separation Method, Method and Formulation for Enzymes, Formulas for Enzymes, Formulae of Enzymes, Enzymic Production of Amino Acids, Method for Production of Enzymic of Amino Acids, Fruit Processing, Small Scale Fruit Processing, Enzyme Industry, Enzyme Industry in India, Enzyme Business, Profitable Biotechnology Business Ideas, Biotechnology Industry in India, Fruit Processing Industry, Fruits Processing Methods, Fruit Processing in India, Methods of Processing Fruits, Enzyme Inhibition, Methods of Purification of Enzymes, Enzyme Purification, Purification of Enzymes, Large-Scale Purification of Enzymes, Enzyme Extraction and Purification Process, Enzyme Purification Methods, Enzyme Biotechnology, Guide to Protein Purification, Cheese Production, Cheese Making Process, Cheese Manufacture, Cheese Production Process, Cheese Production Steps, Manufacture of Cheese, Manufacturing, Cheese, Cheese Making, Cheese Manufacturing
Use of microbes in industry. Production of enzymes-General consideration-Amyl...Steffi Thomas
Industrial uses of microbes, properties of useful industrial microbes, various industrial products, production of enzymes-general consideration-amylase, catalase, peroxidase, lipase, protease, penicillinase, procedure for culturing bacteria and inoculum preparation, submerged fermentation and solid state fermentation, uses of different enzymes
Production of tetracyclin and cephalosporinSamsuDeen12
Tetracyclin and cephalosporins are one of the major used antibiotics commonly all around the world. They are used to treat against microorganisms as a bactericidal, these eliminates those organisms in the host through various mechanism. These antibiotics are produced in a large scale using a bioreactors in many countries.
This PPT will provide the basic idea of Fermentation technology and it's use. The reference book is 'Pharmaceutical Biotechnology' by Giriraj Kulkarni.
production of penicillin b pharma 6th sem by piyush lodhiDrx Piyush Lodhi
project b pharma 6th sem.
Penicillium mold naturally produces the antibiotic penicillin. ... Scientists learned to grow Penicillium mold in deep fermentation tanks by adding a kind of sugar and other ingredients. This process increased the growth of Penicillium.
Explanation on the industrial production of penicillin covering the history, fermentors, specific conditions required for penicillin production, how to increase yield amongst others.
Fermentation of Penicillin Antibiotic
Penicillin is an antibiotic produced by microorganisms. These antibiotics inhibit the growth and development of another micro-organism. Generally, the penicillin antibiotic is produced by some actinomycetes and some filamentous fungi. The antibiotics produced by these micro-organisms can be used medicine field, veterinary as well as agricultural field. Penicillin antibiotic was the first antibiotic used in large amount during world war second for treatment of soldiers. Penicillin is a antibiotic used against Gram positive bacteria as well as high dosage can be used against Gram negative bacteria. Penicillin is not harmful to plants, animals or human beings except in some cases of allergies
This presentation is about what exactly is penicillin and how it was discovered along with its industrial production process from fermentors until yield.
Penicillin is one of the most commonly used antibiotics globally, as it has a wide range of clinical indications. Penicillin is effective against many different types of infections involving gram-positive cocci, gram-positive rods (e.g., Listeria), most anaerobes, and gram-negative cocci (e.g., Neisseria). Importantly, certain bacterial species have obtained penicillin resistance, including enterococci. Enterococci infections now receive treatment with a combination of penicillin and streptomycin or gentamicin. Certain gram-negative rods are also resistant to penicillin due to penicillin’s poor ability to penetrate the porin channel. However, later generations of broad-spectrum penicillins are effective against gram-negative rods. Second-generation penicillins (ampicillin and amoxicillin) can also penetrate the porin channel, making these drugs effective against Proteus mirabilis, Shigella, H. influenzae, Salmonella, and E. coli. Third-generation penicillins such as carbenicillin and ticarcillin are also able to penetrate gram-negative bacterial porin channels. Fourth-generation penicillins such as piperacillin are effective against the same bacterial strains as third-generation penicillins as well as Klebsiella, enterococci, Pseudomonas aeruginosa, and Bacteroides fragilis.
A broad module on industrial microbiology is summarized with pictures .It includes the production of vitamins,vaccine ,alcohol,vinegar,steroids,amino acids ,antibiotics .it also includes the general idea on history ,media,equipment,fermentation,procedure ,uses of industrial microbiology .The production of wine,beer and vinegar are mine core interest .Hope may help ....Thank you .
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
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- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
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263778731218 Abortion Clinic /Pills In Harare ,ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group of receptionists, nurses, and physicians have worked together as a teamof receptionists, nurses, and physicians have worked together as a team wwww.lisywomensclinic.co.za/
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
2. ● First true naturally-occurring antibiotic ever discovered
● Penicillin is a secondary metabolite of certain species of Penicillium
Examples: Amoxicillin, ampicillin, phenoxymethylpenicillin.
What is Penicillin
3. History: Discovery &
Production
● 1928: Scottish biologist, Alexander Fleming
accidently discovered Penicillin. He found
that the fungus Penicillium notatum
prevented growth of bacteria Staphylococcus
spp.
● 1932: Clutterbuck and his colleagues studied
the nature of Penicillin and found it as an
organic acid
4. History: Discovery &
Production
● 1940; Howard Florey and Ernst Chain managed to
purify penicillin in a powdered form.
● 1942:Purified protein was used to treat streptococcal
meningitis
● 1943: Penicillin on a large scale.
This helped immensely to treat casualties during the
WWII that had bacterial infections due to their
wounds.
5. Classes of Penicillins
◦ Natural Penicillins:
◦ Penicillin G (same as Benzylpenicillin)
◦ Penicillin V (same as Phenoxymethylpenicillin)
◦ Aminopenicillins
◦ Ampicillin
◦ Amoxicillin
◦ Hetacillin
◦ Penicillinase-resistant Penicillins (Antistaphylococcal Penicillins)
◦ Methicillin (prototype)
◦ Cloxacillin
◦ Dicloxacillin
◦ Nafcillin
◦ Oxacillin
7. The basic chemical structure of all penicillin group consists of a beta-lactam ring,
a thiazolidine ring, and a side chain (6-aminopenicillanic acid)
Structure ofPenicillin:
8. Penicillin Derivative
●Derivatives produced to deal with the
problemof bacterial resistance to penicillin.
●All penicillin or penicillin derivative have a
constant core region which is the 6-APA.
●The only region that is different from different
types of penicillin derivative is its R group.
11. Biosynthesis of Penicillin:
◦ The catalytic step involves an ACV synthetase enzyme that condenses
the lateral chain of cysteine, valine, and alpha aminoadipate into
tripeptide ACV.
◦ In the second step, tripeptide ACV forms a bicyclic ring by oxidative ring
closure. Isopenicillin N synthase is involved resulting in isopenicillin N
which is a bioactive intermediate in the pathway.
◦ The third step involves the exchange of L-aminoadipate. Acyl-CoA
synthetase and Acyl-CoA racemase, a two enzyme system is involved
that helps in converting isopenicillin N into Penicillin G.
13. Stagesof
Production
1. Primary metabolism will be emphasised. Media for this stage will
be focussed on achieving maximum growth and biomass
production.
2. Once the desired biomass has been achieved, starve (Limiting the
amount of C and N available to the culture) the culture and induce
the kind of stress conditions that trigger the production of the
antibiotic.
★ Use the fed-batch method to feed the culture. As stated above, this
allows us to add the substrate to the reactor in small increments
and to even change the substrate if we so desire.
15. Penicillin production steps:
1. Seed Culture development in laboratory.
2. After three days of incubation, the content is used for inoculation and
kept in a fermentor that is well equipped with optimum conditions.
3. The content is filtered after six days of incubation which contains
penicillin.
4. The penicillin is extracted into amyl or butyl acetate and is transferred
into an aqueous solution with phosphate buffer.
16. Penicillin production steps:
5. Acidify the extract and again re-extract penicillin into butyl acetate
6. In the solvent extract potassium acetated is added to a crystallization
tank to crystallize as a potassium salt.
7. Crystals were recovered and further sterilization of salt is done.
18. Strain Improvement
• At the end of the WWII, penicillin was first made using the fungus
Penicillium notatum, which produced a yield of 1mg/dm3
● Today, using a different species known as Penicillium
chrysogenum, and better extraction procedures, the yield is 50
mg/dm3
21. ★ Medium for
penicillin
1. The Penicillium chrysogenum usually contain its carbon source
which is found in corn steep liquor and glucose.
2. A medium of corn steep liquor and glucose are added to the
fermenter. Medium also consists of salts such as MgSO4, K3PO4 and
sodium nitrates. They provide the essential ions required for the
fungus metabolic activity.
22. Production Method
● Secondary metabolites are only produced in times of stress when
resources are low and the organism must produce these compounds
to kill off its competitors to allow it to survive.
● It is these conditions that we wish to duplicate in order to achieve
the maximum amount of product from our fermentation.
23. Heat sterilization
3.Medium is sterilized at high heat and high pressure, usually through a
holding tube or sterilized together with the fermenter.
4.The pressurized steam is used and the medium is heated to 121°C at 30
psi or twice the atm. pressure
24. Seed Culture
9. The seed culture is developed first in the lab by the addition of
Penicillium chrysogenum spores into a liquid medium. When it has
grown to the acceptable amount, it is inoculated into the fermenter.
10. The medium is constantly aerated and agitated. Carbon and
nitrogen are added sparingly alongside precursor molecules for
penicillin fed-batch style. Typical parameters such as pH, temperature,
stirrer speed and dissolved oxygen concentration, are observed.
25. Seed Culture
11. After about 40 hours, penicillin begins to be secreted by the fungus.
12.After about 7 days, growth is completed, the pH rises to 8.0 or above
and penicillin production ceases.
The Penicillium fungus
26. Fermentation:
5.It is done in a fed-batch mode as glucose must not be added in high
amounts at the beginning of growth (which will result in low yield of
penicillin production as excessive glucose inhibit penicillin production).
6.The fermentation conditions for the Penicillium mold, usually
requires temperatures at 20-24°C while pH conditions are kept at 6.5
7.The pressure in the bioreactor is much higher than the atmospheric
pressure (1.02atm). This is to prevent contamination from occurring as it
prevents external contaminants from entering.
27. Fermentation
It is necessary to mix the culture evenly throughout the culture
medium. Fungal cells are able to handle rotation speed of around 200
rpm.
Fermentors
28. Product Recovery
• Harvest broth from fermented tank by filtration (rotary vaccum
filtration) chill to 5-10 C (because penicillin is highly reactive and
destroyed by alkali and enzyme)
• Acidify filtrate to pH 2.0-2.5 with H2SO4 ( to convert penicillin to its
anionic form)
• Extract penicillin from aqueous filtrate into butyl acetate or amyl
acetate (at this very low pH as soon as possible in centrifugal
counter current extractor)
• Discard aqueous fraction
Rotary vacuum filter
29. ◦ Allow the organic solvent to pass through charcoal
to remove impurities and extract penicillin from
butylacetate to 2% aqueous phosphate buffer at pH
7.5
◦ Acidify the aq. Fraction to pH 2-2.5 and re-extract
penicillin into fresh butylacetate ( it concentrated up
to 80-100 times)
◦ Add potassium acetate to the solvent extract in a
crystallization tank to crystalize as potassium salt
◦ Recover crystal in filter centrifuge
◦ Sterilization
◦ Further processing
◦ Packaging
30. Storage
Penicillin is stored in containers and kept in a dried environment.
The White Penicillin-Sodium salt
The resulting penicillin (called Penicillin G) can be chemically and
enzymatically modified to make a variety of penicillins with slightly
different properties.
These can be semi-synthetic penicillins, such as; Penicillin V,
Penicillin O, ampicillin and amoxycillin.
31. SpecificConditions forPenicillin
Production
● Most penicillins form filamentous broths. This means they can be
difficult to mix due to their high viscosity. Also the increasing
viscosity of the broth can hinder oxygen transfer.
32. A solution for the viscosity and the
filamentous growth of penicillium
species could be bubble columns (air lift
reactors) which would distribute the
oxygen equally and also to agitate the
medium.
SpecificConditions forPenicillin
Production
33. ● Penicillin is an aerobic organism; oxygen supply is critical: reactor
must have an efficient oxygen supply system.
● The optimum pH for penicillin
efficiently (pH controller
growth is 6.5: maintain pH
and acid-base reservoir).
● Strain Stability problems (mutations): careful strain maintenance is
required.
● Biomass doubling is about 6h: provisions must be made.
SpecificConditions forPenicillin
Production