This document discusses primary cells and their use in biomedical research. Primary cells are isolated directly from tissues and have important advantages over cell lines, including greater biological relevance. Primary cells are used in applications such as biomarker research, drug development, immunotherapeutics, and tissue engineering. The document reviews techniques for working with primary cells, including isolation, culture, differentiation, and characterization using methods like flow cytometry and immunoassays. Primary cells provide a more physiologically relevant model for research compared to cell lines.
Cell Storage Best Practices: Techniques to Ensure Greater Research Resultsallcells
Featuring: Cryopreservation Specialist, Michael George
This webinar will introduce cell storage equipment and techniques in order to provide the most up-to-date trends in research today. There will be special emphasis on human primary cell storage, cryopreservation, and transportation.
Topics to be covered in the webinar:
• Background on life science storage methods
• Advantages/disadvantages of storage equipment
• What not to do (common mistakes/misconceptions)
• Interview with James Lee, Associate Director of Production, AllCells
• Q&A
What process is used to obtain cells from bone marrow and normal peripheral blood?
What is the best cell counting and viability method for primary cells?
AllCells, your primary cells research partner, and Nexcelom Bioscience, your cell counting experts, have joined together in an exclusive collaboration to host a free webinar to help educate researchers and present data from their own experiences.
Stem cells are one of the important cells present in both plant and animals. these cells have ability to regenerate any part of the body work similarily as meristem cells in plant. The advances in the stem cell technology has open a new era in medical field. the advances in this technology has been presented here and their important application has been included in this present in this presentation.
Cell Storage Best Practices: Techniques to Ensure Greater Research Resultsallcells
Featuring: Cryopreservation Specialist, Michael George
This webinar will introduce cell storage equipment and techniques in order to provide the most up-to-date trends in research today. There will be special emphasis on human primary cell storage, cryopreservation, and transportation.
Topics to be covered in the webinar:
• Background on life science storage methods
• Advantages/disadvantages of storage equipment
• What not to do (common mistakes/misconceptions)
• Interview with James Lee, Associate Director of Production, AllCells
• Q&A
What process is used to obtain cells from bone marrow and normal peripheral blood?
What is the best cell counting and viability method for primary cells?
AllCells, your primary cells research partner, and Nexcelom Bioscience, your cell counting experts, have joined together in an exclusive collaboration to host a free webinar to help educate researchers and present data from their own experiences.
Stem cells are one of the important cells present in both plant and animals. these cells have ability to regenerate any part of the body work similarily as meristem cells in plant. The advances in the stem cell technology has open a new era in medical field. the advances in this technology has been presented here and their important application has been included in this present in this presentation.
This presentation deals with stem cell therapy & new avenues in stem cell therapy. It also discusses latest advances such as treatment against baldness, multiple sclerosis, type 1 diabetes, spinal cord injury, demyelinating diseases, deafness, eye, Parkinson's disease. Also discusses about umbilical cord stem cells and finally clinical trials without patients (organs on chips).
Cellular coning refers to generation of genetically identical cells from parent cells. This presentation teaches differences between cell coning and molecular cloning and various methods of cell cloning. Sample questions are also provided for your review of concept learned
Stem cells are the cells which have the capability to differentiate into any cells of the body when provided with right stimulus and environment. This presentation teaches about stem cells, characteristics, types and cultivation of stem cells in artificial environment. Sample practice questions are also provided in the end to review the concept learned from this presentation.
EU: Primary Cells and Primary Batteries – Market Report. Analysis and Forecas...IndexBox Marketing
IndexBox Marketing has just published its report: “EU: Primary Cells and Primary Batteries - Market Report. Analysis And Forecast To 2020”. This report focuses on the EU primary cell and battery market, providing a comprehensive analysis and the most recent data on its market size and volume, EU trade, price dynamics, domestic production, and turnover in the industry. The market trends section reveals the main issues and uncertainties concerning the industry, while the medium-term outlook uncovers market prospects. The attractivity index (IB Index) summarizes the source of existing opportunities as they appear in this market, as well as an interpretation of the trade figures.
This presentation deals with stem cell therapy & new avenues in stem cell therapy. It also discusses latest advances such as treatment against baldness, multiple sclerosis, type 1 diabetes, spinal cord injury, demyelinating diseases, deafness, eye, Parkinson's disease. Also discusses about umbilical cord stem cells and finally clinical trials without patients (organs on chips).
Cellular coning refers to generation of genetically identical cells from parent cells. This presentation teaches differences between cell coning and molecular cloning and various methods of cell cloning. Sample questions are also provided for your review of concept learned
Stem cells are the cells which have the capability to differentiate into any cells of the body when provided with right stimulus and environment. This presentation teaches about stem cells, characteristics, types and cultivation of stem cells in artificial environment. Sample practice questions are also provided in the end to review the concept learned from this presentation.
EU: Primary Cells and Primary Batteries – Market Report. Analysis and Forecas...IndexBox Marketing
IndexBox Marketing has just published its report: “EU: Primary Cells and Primary Batteries - Market Report. Analysis And Forecast To 2020”. This report focuses on the EU primary cell and battery market, providing a comprehensive analysis and the most recent data on its market size and volume, EU trade, price dynamics, domestic production, and turnover in the industry. The market trends section reveals the main issues and uncertainties concerning the industry, while the medium-term outlook uncovers market prospects. The attractivity index (IB Index) summarizes the source of existing opportunities as they appear in this market, as well as an interpretation of the trade figures.
Transfection is the process of introduction of foreign DNA into the nucleus of eukaryotic cell. The cells which has incorporated exogenous DNA are called transfectants.
There are two types of Transfection possible,
Transient and
Stable Transfection.
In transient Transfection, the foreign DNA will not get incorporated in to the host genome, but genes are expressed for limited period of time (24-96 hrs).
Stable transfectants will have the foreign DNA incorporated into the genome.
In the development of cancer immunotherapies, vaccines, and autoimmune disease treatments, researchers are increasingly aware of the importance of host human leukocyte antigens (HLA) in understanding the complexity of immune responses. HLA allelic variants have distinct binding affinities for peptide antigens during presentation, and thus regulate auto-antigen and pathogen-specific T cell activation. HLA polymorphisms have been correlated with disease risk, immune responses to infectious disease, as well as adverse responses to therapies. In this webinar, we will review the genetics and biology of HLA types and explain the technology underlying HLA identification. We will also provide some examples of how HLA-typing can be a valuable investment during early stage drug-development in human primary cells.
Plant biopharming is defined as the farming of transgenic plants genetically modified to produce “humanised” pharmaceutical substances for use in humans.
stem cell research is yet to be advanced , once fully developed can alleviate human suffering, this ppt reviews the contemporary evidence, pitfalls and challenges
Creative Bioarray offers 35 human cell systems with over 160 different cell types. Moreover, we also provide our customers with primary cells from over 13 types of other animals.
https://www.creative-bioarray.com/products/primary-cell-types-list-14.htm?page=1
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Toxic effects of heavy metals : Lead and Arsenicsanjana502982
Heavy metals are naturally occuring metallic chemical elements that have relatively high density, and are toxic at even low concentrations. All toxic metals are termed as heavy metals irrespective of their atomic mass and density, eg. arsenic, lead, mercury, cadmium, thallium, chromium, etc.
Salas, V. (2024) "John of St. Thomas (Poinsot) on the Science of Sacred Theol...Studia Poinsotiana
I Introduction
II Subalternation and Theology
III Theology and Dogmatic Declarations
IV The Mixed Principles of Theology
V Virtual Revelation: The Unity of Theology
VI Theology as a Natural Science
VII Theology’s Certitude
VIII Conclusion
Notes
Bibliography
All the contents are fully attributable to the author, Doctor Victor Salas. Should you wish to get this text republished, get in touch with the author or the editorial committee of the Studia Poinsotiana. Insofar as possible, we will be happy to broker your contact.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
2. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Webinar Outline
•What are primary cells?
•Primary Cells vs Cell Lines
•Examples of primary cell applications
•How to work with primary cells
•Q&A
3. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
The diverse history of Cell Culture
1885:
Chicken embryology
1940s:
Vaccines
1960s: Embryonic
stem cells
1970s: Recombinant DNA
1951
HeLa
Cell Line
4. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
What are Primary Cells?
Subculture/ passage
transformationtumor
• Cell Lines
• Continuous Cell lines
• Primary Cells
• Primary Hematopoietic Cells
Lymphocytes
HeLa Cells
5. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Primary cells vs. Cell lines
Biological relevanceBiological relevance
Uniform cell type
Chromosomal aberrations
Identity is questionable
Heterogeneous cell population (PB-MNC)
Variability
Can select specific donor characteristics
Can order many vials from a single donor
6. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Primary cells vs. Cell lines
Access and Ease of useAccess and Ease of use
Low cost (buy once)
Easy to keep alive
Frozen or fresh
High viability
No specialized media
Limited lifespan
7. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Primary cells vs. Cell lines
Applications
Cell Biology
Disease mechanisms
Drug development
Biomarkers
Tissue engineering
Reagent development
Manufacturing/ Industrial
8. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Examples of Primary Cell Applications
1. Biomarkers
•$53.6 bn (2018)
•Cancer biomarkers-up 18.26% by 2019
2. Drug screening
•$16.6 bn (2015)
3. Immuno-therapeutics
• $67.9 bn (2018)
4. Tissue Engineering
•$10.6 bn (2018)
•Americas hold largest market share
Source: MarketWatch (Dow Jones)
10. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Biomarker Research: Drug Resistance
Gandhi, et al. (2014) British Journal of Haematology. 164: 233-244.
• Immunomodulatory drugs (IMiDs) are used to treat
hematological pathologies
• Cereblon mRNA levels correlate with IMiD resistance
Wild Type IMiD Resistant
Microscopy of Cell lines
Cereblon levels vs. Drug resistance
Wild Type IMiD Resistant
100 um
CD138+ Multiple Myeloma Bone MarrowCD138+ Multiple Myeloma Bone Marrow
11. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Drug Screening: Cytotoxicity Micro-arrays
• Test drug compounds for effects on cells
• Detect cell death, phenotype changes, specificity, protein expression
12. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Drug Screening: Cytotoxicity Micro-arrays
1. Fabricate DNA micro-spots
2. Cancer cell death after 16 hr α-CD20 treatments
3. Drug dosage data
100 um
Blue-Live cancer cells
Purple-Dead cancer cells
Red- Dead PBMNC
Hsiao SC, Liu H, Holstlaw TA, Liu C, Francis CY, et al. (2013) PloS ONE 8(6) e66739.
Peripheral Blood Mononuclear CellsPeripheral Blood Mononuclear Cells
16. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Tissue Engineering: Vascularized Grafts
• A novel methodology for co-implantation of CD34+ hematopoietic progenitors
and mesenchymal stem cells (MSC)
Moioli, E. K. et al. (2008) PLoS One. 3 (12); e3922.
Bone Marrow, Bone Marrow CD34+Bone Marrow, Bone Marrow CD34+
17. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
How to work with Primary Cells
• Cell Isolation and Handling Procedures
• Tissue culture, differentiation and characterization
• Downstream processing
18. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Purified Cell Isolation and Handling
Instructional videos on cell handling available at AllCells.com
Immunomagnetic cell separation Whole Tissue Isolation
Freezing Primary CellsThawing Primary Cells
19. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Tissue Culture, Differentiation, Characterization
100 um 100 um
T-cell activation
Macrophage polarization
M2 Phenotype100 um
CD68 CD80
CD163CD68
Inactive Active
M1 Phenotype
Lineage Tree
20. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Tissue Culture, Differentiation, Characterization
Multipotency of Bone Marrow Mesenchymal Stem Cells
100 um
Microscopy images of differentiation
Bench-top bioreactors for MSC tissue engineering
Perfusion co-cultures
Mechanical-based cues during culture
Lineage Tree
Cardiomyocytes
21. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Tissue Culture, Differentiation, Characterization
TargetA-APC
Target B-PE
TargetC-FITC
TargetE-PacificBlue
Target F-APC-CY7
Propidium Iodide (PerCP)
SSC-A
Multi-color Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS)
Data readout:
BD FACS Canto II
Target D-PE-CY7
BD FACS Aria III
Relative equipment:
Cell Sorting
22. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Tissue Culture, Differentiation, Characterization
Bio Rad Bio-plex 200
Meningococcal Vaccine Research
Multiplex Luminex Cytokine analysis
Relevant Equipment:
Example:
Applications:
Mechanism:
23. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Conclusions
• Primary cells lines provide a more relevant system for biomedical
research in contrast to cell lines
• Primary cells are sourced via immunomagnetic separation and
whole tissue isolation
• Primary cells are used in multifaceted research involving
biomarkers, drug development, immunology and tissue engineering
• After isolation, primary cells can be cultured, differentiated and
characterized using flow cytometry, immunoassays, and many other
techniques
24. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Erin Kelly, PhD.
Bioservices Supervisor
Please direct offline questions to ekelly@allcells.com or
sales@allcells.com
www.allcells.com/technical-support/
www.allcells.com/support/webinars/
Contact Information
Editor's Notes
Four major areas of primary cell research are the field of biomarkers, drug screening, immunotherapeu
Chen and Tuan Arthritis Research & Therapy 2008 10:223
Kuhn Lab, Harvard University