This document provides an overview of HLA typing and its applications in research. It begins with a brief introduction to HLA molecules and their role in presenting peptides to T cells. It then discusses the diversity of HLA alleles and the evolution of HLA typing techniques. The main applications of HLA typing are described as transplantation, forensics, biomarker identification, vaccine development, and studying associations with autoimmune diseases. The document emphasizes that HLA typing performed by AllCells on primary human cells can accelerate immunology and vaccine research.

1. Your Reliable Source of Human Primary Cells
Donor HLA Typing 101:
How HLA Characterization
Can Accelerate Your Research

2. HLA – What is it?
HLA typing technology
Applications
Q&A
1
2
3
4

3. Macrophage or APC
B cells
foreign
protein
peptide / HLA
T cells
Activated T cells
60-sec Immunology Review

4. ER
Major
Histocompatibility
Complex (MHC)
Antigen-specific T cell
T cell
Receptor
(TCR)
Human Leukocyte Antigen
(HLA)
HLA molecules “present” peptide to T cells

5. CD4 T helper cell
HLA Class IIHLA Class I
CD8+ Cytotoxic T cell
Cytosolic proteins
Proteosome
All nucleated cells
Extracellular pathogens
(except cross-presentation)
Lysosome
Only APCs
13-25 aa 9-22 aa
2 flavors of HLA

6. CLASSI
1
CLASSII
HLA diversity is the result of genetic
polymorphisms
HLA-B8
*0801
*0802
*0803
…..
*0821
HLA- B > 3,000 known alleles

7. • Crossmatch
• Microcytotoxicity Test
• Mixed Lymphocyte Reaction Assay
• Immunophenotyping
• SSP Genotyping
• SSO Genotyping
Evolution of HLA Typing

8. SSO Genotyping Method

9. HLA Data Analysis

10. Transplantation
Forensics
Disease-related Biomarkers
HLA-typing applications
Vaccine Development

11. HLA and Autoimmune disease
Disease HLA Allele
Celiac Disease HLA-DQ8, -DQ2
Type I Diabetes HLA-DRB1*04:01
Multiple Sclerosis HLA-DRB1*15:01
RA (ankylosing
spondylitis)
B27
Behçet's disease HLA-B51

12. Transplantation
Forensics
Disease-related Biomarkers
HLA-typing applications
Immunogenicity
Vaccine Development

13. HLA and Immunogenicity
• HLA-B*57:01
Ostrov et al, PNAS, 2012

14. AllCells HLA-typed
Primary Cells*
AllCells
Immunoassays
PBMC
T cells
B cells monocytes
Cytokine Release Assays
MLR Assays
T cell proliferation
T helper cell polarization
HLA typing at AllCells
* Primary cells from healthy donors or diseased patients

15.  Bruce Sun
Sr. Manager, Product and Application Development
Please direct offline questions to bsun@allcells.com or
sales@allcells.com
www.allcells.com/technical-support/
www.allcells.com/support/webinars/
Contact Information