This research paper investigates the uptake and transport of furanodiene in Caco-2 cell monolayers, comparing its performance with furanodiene-loaded PLGA and PEG-PLGA nanoparticles. The study demonstrates that the modified nanoparticles enhance the stability and bioavailability of furanodiene, addressing its low aqueous solubility and permeability. Overall, the findings suggest improved drug delivery potential through these nanoparticle formulations.

1. Seminar on,
Recent Trends in Pharmaceutical Sciences
(Research Paper Title: Uptake and transport of furanodiene in Caco-2 cell
monolayers: a comparison study between furanodiene and furanodiene
loaded PLGA nanoparticles)
BY:
Nitin P. Kanwale
Roll no. 06
M. Pharm. Sem III
Dept. QAT
UNDER GUIDANCE OF:
Dr. Mrudula H. Bele
(Pharmaceutics department)
MVP Samaj's college of Pharmacy, Nashik
MVP Samaj’s College of
Pharmacy, Nashik05-11-2015 1

2. 1. Drug profile
2. Introduction
3. Preparation of FDE-PLGA-NPs and FDE-PEG-PLGA-
NPs:
4. Characterization of FDE-PLGA-NPs And FDE-PEG-
PEGA-NPs:
5. Conclusion
6. References
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3. 3
FURANODIENE
Stucture
Chemical name
(5E,9E)-3,6,10-trimethyl-4,7,8,11-
tetrahydrocyclodeca[b]furan
Pharmacological
activities
Anti-tumor, Anti-inflammation.
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4. RESEARCH QUESTION ?
As it possible to improve the stability and bioavailability of FDE by preparing
FDE loaded PLGA and PEGylated PLGA nanoparticles ?
GOAL OF WORK :
furanodiene has some disadvantages like low aqueous solubility, low
stability, low permeability, uptake by cell so there is need to improve the
stability and oral bioavailability of furanodiene.
Use Caco-2 cell line as a in vitro model to evaluate the response of Caco-2
cells to FDE, FDE-PLGA-NPs and FDE-PEG-PLGA-NPs to compare the
capacity of the carrier to mediate FDE transport across cell monolayers.
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5. • By using modified spontaneous emulsion solvent diffusion
method:
3 mg furanodiene+25 mg PLGA(PEG-PLGA) dissolved in 2 ml acetone
slowelly injected into
15 ml of 0.2% tween80 solution
magnetic stirring at room temp. to evaporate the organic
solvent.
nanoparticle suspension was collected by centrifugation(15000 rpm/min.)
wash 3 times by using deionized water to remove
excessive
emulsifier.
nanoparticle suspension then freeze dried to obtain a fine power of
nanoparticle.
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6. • 4.1Nanoparticle size, size distribution, zeta potential, surface morphology,
entrapment efficiency:
EE= (T-F) /T×100
Where: T= total amount of furanodiene added
F= free furanodiene amount in the supernatant
Table1: Characteristics of the nanoparticles
Fig. 1 Scanning electron microscope (SEM) images of the nanoparticles. Scale bar = 200 nm. (a) FDE-PLGA-NPs,
(b) FDE-PEG-PLGA-NPs
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7. 05-11-2015 7
Fig. 2: Stabilities of FDE, FDE-PLGA-NPs and FDE-PEG-PLGA- Fig. 3: Stabilities of FDE, FDE-PLGA-
NPs and FDE-
NPs in PBS (pH 7.4). PEG-PLGA-NPs in artificial gastric
fluids.
Fig. 4: Stabilities of FDE, FDE-PLGA-NPs and FDE-PEG-PLGA-NPs in artificial
intestinal fluids.

8. Fig. 5: The cytotoxicity of FDE, FDE-PLGA-NPs and FDE-PEG-PLGA-NPs.
Caco-2 cells were incubated with sample solutions (1 to 40 μg−1 of FDE) for
6 h. Cell viability was determined by MTT assay.
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9. Fig. 6: Quantitative analysis of FDE cellular uptake. Caco-2 cells were
incubated with sample solutions (15 μg·mL−1 of FDE) for different durations.
Intracellular FDE was assayed by RP-HPLC.
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10. Where: Papp= Apparent permeability coefficient
dq= amount of FDE in the receptor compartment at time t.
A = diffusion area
C0 = initial concentration of FDE in the donor compartment.
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Papp=
𝑑𝑄
𝑑𝑡
×
1
𝐴×𝐶0

11. Fig. 7: Transport of FDE across Caco-2 cell monolayers (AP to BL ).
Fig. 9: Transport of FDE across Caco-2 cell monolayers (BL to AP ).
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12. These results indicated that FDE-PLGA-NPs, especially
FDE-PEG-PLGA-NPs, could enhance the stability and
hydrophilicity of FDE and increase the permeation of FDE
across Caco-2 cell monolayers.
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OVER VIEW:
POSITIVE POINT:
NEGATIVE POINT:

13. 1. Gang L, Dong-hai L, Xin-xin X, Li-fang Q, Jun-teng W, Ke L. Uptake
And Transport Of Furanodiene In Caco-2 Cell Monolayers: A
Comparison Study Between Furanodiene And Furanodiene Loaded
PLGA Nanoparticles. Chinese Journal Of Natural
Medicines2013;11(1):049-055.
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14. 14
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