Mercury pollution in the aquatic environment can cause intoxication of marine
organisms and the formation of free radicals in the human body if consumed. Curcumin
is a natural ingredient that contains extracellular antioxidants to overcome the
formation of free radicals in the body. The purpose of the study was to determine the
effect of administering curcumin to the number of pyramid cells that were necrotic in
mice (Mus musculus) exposed to methylmercury. This experiment used a completely
randomized design with 4 groups of mice, i.e. 0.5 ml distilled water, 0.056 mg/kg
methylmercury, 0.0056 mg/kg methylmercury + 150 mg/kg curcumin and 0.0056 mg/kg
methylmercury + 300 mg/kg curcumin. Examination of total pyramid cell necrosis was
observed with a 400x magnification light microscope. In this study, there was a
decrease in the number of pyramid cell necrosis in mice. The decrease in the number
of necrotic cells was directly proportional to the increase in dose given. The treatment
results as positive controls showed the highest value with the number of pyramid cells
experiencing necrosis of 23.28. The highest decreases in the number of each treatment
were 10.32 in 150 mg/kgBW curcumin and 5.80 in 300 mg/kgBW curcumin. Curcumin
can reduce the number of pyramid cells that experience necrosis due to exposure to
methylmercury.
The document summarizes a study that examined histopathological changes in rat testes, liver, kidney, and brain tissues after acute oral administration of boric acid. Rats were given 1000 mg/kg/day of boric acid for 7 days. Significant weight loss and organ weight reductions were observed in treated rats compared to controls. Histopathological examination found edema, cellular degeneration, and inhibited spermatogenesis in testes as well as edema in brain tissue of treated rats. The study concludes that acute boric acid administration caused widespread toxic effects and histopathological changes, especially by inhibiting spermatogenesis in testicular tissue.
Maternal curcumin exposure causes fetal gross morphological anomalies and ske...Innspub Net
Curcumin is a phenolic compound extracted from the rhizome of turmeric (Curcuma longa L.). Although declared as safe for human consumption, curcumin has been found to be embryotoxic in some organisms indicating its potential as a teratogen. In this study, the teratogenic effect of maternal curcumin exposure in mouse fetuses was evaluated. Three experimental groups of pregnant mice were treated with 1.05, 1.52, and 2.0 mg/g body weight/day 95% curcumin, respectively, from gestation day (GD) 6 to 15. A fourth group without curcumin exposure served as a control. At GD18, the mice were sacrificed and the total number of implanted embryos including resorbed, dead, and live fetuses were counted for litter analysis. Extracted fetuses were also analyzed for gross morphological anomalies and subsequently have undergone alizarin staining for the visualization of skeletal malformations. Results showed an increased resorption rate in the 2.0 mg/g treatment (p<0.001). There is also a reduction of fetal weight (p<0.001) and crown-rump length (p<0.001) in a dose-dependent manner. Gross morphological analysis shows cranio-facial malformations such as flattened nose bridge (p<0.05) and micrognathia (p<0.05) in 2.0 mg/g treatment. Skeletal malformations such as large anterior fontanelle (p<0.001), misaligned ossification centers in the sternum (p<0.001), and delayed ossification in the forepaws, hind paws, and caudal vertebrae (p<0.001) were also observed at 2.0 mg/g treatment. Meanwhile, the presence of supernumerary ribs is not statistically different in the four groups. The results indicate that curcumin is teratogenic in mouse fetuses due to observed gross morphological anomalies and skeletal malformations.
The document reports on a study investigating the role of the p38 mitogen-activated protein kinase p38γ in Alzheimer's disease (AD) pathogenesis. The study found that depletion of p38γ exacerbated neuronal excitotoxicity, cognitive deficits, neuronal circuit abnormalities, and premature mortality in an AD mouse model overexpressing amyloid-β (Aβ). In contrast, increasing p38γ activity abolished these Aβ-induced deficits. Furthermore, mimicking site-specific tau phosphorylation by p38γ alleviated Aβ-induced neuronal death and excitotoxicity. The findings suggest p38γ phosphorylation of tau at specific sites inhibits Aβ toxicity in early AD, challenging the view that tau phosphorylation is purely pathogenic.
Effect of sodium selenite and vitamin e on the renal cortex in rats an ultras...Prof. Hesham N. Mustafa
This study examined the effects of sodium selenite and vitamin E supplementation on renal cortex tissue in rats. Rats were divided into groups receiving sodium selenite alone for 2 or 4 weeks, or sodium selenite with vitamin E for 2 or 4 weeks. Kidney tissue was analyzed for changes at the microscopic and ultrastructural levels. Sodium selenite alone caused damage to renal structures like Bowman's capsule and proximal convoluted tubules. Vitamin E supplementation helped reduce this damage and improved cellular integrity when given alongside sodium selenite. The study suggests vitamin E may help alleviate toxic effects from sodium selenite overdose.
Abstract
Objective(s):
Gold nanoparticles (GNPs) command a great deal of attention for biomedical applications nowadays. The data about the degree of toxicity and the accumulation of gold nanoparticles in-vivo is not enough to judge.
Materials and Methods:
A total of 32 healthy male Wistar rats were randomly divided into 4 including: three GNP-treated and one control group. Groups 1, 2 and 3 received 0.5 cc of a solution containing 5, 10, and 100 ppm Au daily via intraperitoneal (IP) injection for 7 days, respectively. The control group was treated with 0.5 cc normal saline with same procedure. Then, several biochemical parameters such as serum glutamate oxaloacetat transaminase (SGOT) and serum glutamate pyrvate transaminase (SGPT) were evaluated at 2, 7 and 14 days after the last injection. After 14 days, all the rats were sacrificed and liver, lung tissues were separated and evaluated.
Results:
SGOT two days after intervention was significantly greater in the group 2 than the control group. In liver histological assessment, in group 1, basophils were observed around the central veins, in group 2 fading and no observation of central veins was seen, and in group 3 hepatic damage was noticed. The lung histological results showed severe vascular hyperemia in group 1, air sacs damage in group 2, and complete air sacs destruction in group 3.
Conclusion:
The results showed extreme changes in the histopathology of lung and liver tissues caused by spherical nanogold with 5-10 nm size in all of three treatment groups.
Objective(s):
There is a rising use of gold nanoparticles (AuNPs) in goods and in the medical fields but there is concern about the toxicity of them. So in this study spherical AuNPs with 3 different concentrations were applied for investigating their effects in vivo.
Materials and Methods:
40 male albino mice were randomly divided into sham, control, 25 ppm, 50 ppm, 100 ppm groups and were treated by intraperitoneal injection for period of 14 days. Blood was taken for measuring of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase (SGOT and SGPT) enzyme levels and Complete Blood Count (CBC).
Results:
After the treatment and comparing groups with sham group, in 50 ppm group significant increases on RBC, HCT, HGB, MCHC and in 25 ppm group significant increase on MCHC and significant decrease on MCV and in 100 ppm group significant increase on MCHC were observed. Also in 50 ppm group an increase on SGOT enzyme level was observed. However, it was nonsignificant.
Conclusion:
By observing the abnormality on the RBC count and SGOT enzyme level in the 50 ppm group, we concluded a slight toxicity effect for AuNPs and the threat potential of their use in human.
The document summarizes a study that examined histopathological changes in rat testes, liver, kidney, and brain tissues after acute oral administration of boric acid. Rats were given 1000 mg/kg/day of boric acid for 7 days. Significant weight loss and organ weight reductions were observed in treated rats compared to controls. Histopathological examination found edema, cellular degeneration, and inhibited spermatogenesis in testes as well as edema in brain tissue of treated rats. The study concludes that acute boric acid administration caused widespread toxic effects and histopathological changes, especially by inhibiting spermatogenesis in testicular tissue.
Maternal curcumin exposure causes fetal gross morphological anomalies and ske...Innspub Net
Curcumin is a phenolic compound extracted from the rhizome of turmeric (Curcuma longa L.). Although declared as safe for human consumption, curcumin has been found to be embryotoxic in some organisms indicating its potential as a teratogen. In this study, the teratogenic effect of maternal curcumin exposure in mouse fetuses was evaluated. Three experimental groups of pregnant mice were treated with 1.05, 1.52, and 2.0 mg/g body weight/day 95% curcumin, respectively, from gestation day (GD) 6 to 15. A fourth group without curcumin exposure served as a control. At GD18, the mice were sacrificed and the total number of implanted embryos including resorbed, dead, and live fetuses were counted for litter analysis. Extracted fetuses were also analyzed for gross morphological anomalies and subsequently have undergone alizarin staining for the visualization of skeletal malformations. Results showed an increased resorption rate in the 2.0 mg/g treatment (p<0.001). There is also a reduction of fetal weight (p<0.001) and crown-rump length (p<0.001) in a dose-dependent manner. Gross morphological analysis shows cranio-facial malformations such as flattened nose bridge (p<0.05) and micrognathia (p<0.05) in 2.0 mg/g treatment. Skeletal malformations such as large anterior fontanelle (p<0.001), misaligned ossification centers in the sternum (p<0.001), and delayed ossification in the forepaws, hind paws, and caudal vertebrae (p<0.001) were also observed at 2.0 mg/g treatment. Meanwhile, the presence of supernumerary ribs is not statistically different in the four groups. The results indicate that curcumin is teratogenic in mouse fetuses due to observed gross morphological anomalies and skeletal malformations.
The document reports on a study investigating the role of the p38 mitogen-activated protein kinase p38γ in Alzheimer's disease (AD) pathogenesis. The study found that depletion of p38γ exacerbated neuronal excitotoxicity, cognitive deficits, neuronal circuit abnormalities, and premature mortality in an AD mouse model overexpressing amyloid-β (Aβ). In contrast, increasing p38γ activity abolished these Aβ-induced deficits. Furthermore, mimicking site-specific tau phosphorylation by p38γ alleviated Aβ-induced neuronal death and excitotoxicity. The findings suggest p38γ phosphorylation of tau at specific sites inhibits Aβ toxicity in early AD, challenging the view that tau phosphorylation is purely pathogenic.
Effect of sodium selenite and vitamin e on the renal cortex in rats an ultras...Prof. Hesham N. Mustafa
This study examined the effects of sodium selenite and vitamin E supplementation on renal cortex tissue in rats. Rats were divided into groups receiving sodium selenite alone for 2 or 4 weeks, or sodium selenite with vitamin E for 2 or 4 weeks. Kidney tissue was analyzed for changes at the microscopic and ultrastructural levels. Sodium selenite alone caused damage to renal structures like Bowman's capsule and proximal convoluted tubules. Vitamin E supplementation helped reduce this damage and improved cellular integrity when given alongside sodium selenite. The study suggests vitamin E may help alleviate toxic effects from sodium selenite overdose.
Abstract
Objective(s):
Gold nanoparticles (GNPs) command a great deal of attention for biomedical applications nowadays. The data about the degree of toxicity and the accumulation of gold nanoparticles in-vivo is not enough to judge.
Materials and Methods:
A total of 32 healthy male Wistar rats were randomly divided into 4 including: three GNP-treated and one control group. Groups 1, 2 and 3 received 0.5 cc of a solution containing 5, 10, and 100 ppm Au daily via intraperitoneal (IP) injection for 7 days, respectively. The control group was treated with 0.5 cc normal saline with same procedure. Then, several biochemical parameters such as serum glutamate oxaloacetat transaminase (SGOT) and serum glutamate pyrvate transaminase (SGPT) were evaluated at 2, 7 and 14 days after the last injection. After 14 days, all the rats were sacrificed and liver, lung tissues were separated and evaluated.
Results:
SGOT two days after intervention was significantly greater in the group 2 than the control group. In liver histological assessment, in group 1, basophils were observed around the central veins, in group 2 fading and no observation of central veins was seen, and in group 3 hepatic damage was noticed. The lung histological results showed severe vascular hyperemia in group 1, air sacs damage in group 2, and complete air sacs destruction in group 3.
Conclusion:
The results showed extreme changes in the histopathology of lung and liver tissues caused by spherical nanogold with 5-10 nm size in all of three treatment groups.
Objective(s):
There is a rising use of gold nanoparticles (AuNPs) in goods and in the medical fields but there is concern about the toxicity of them. So in this study spherical AuNPs with 3 different concentrations were applied for investigating their effects in vivo.
Materials and Methods:
40 male albino mice were randomly divided into sham, control, 25 ppm, 50 ppm, 100 ppm groups and were treated by intraperitoneal injection for period of 14 days. Blood was taken for measuring of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase (SGOT and SGPT) enzyme levels and Complete Blood Count (CBC).
Results:
After the treatment and comparing groups with sham group, in 50 ppm group significant increases on RBC, HCT, HGB, MCHC and in 25 ppm group significant increase on MCHC and significant decrease on MCV and in 100 ppm group significant increase on MCHC were observed. Also in 50 ppm group an increase on SGOT enzyme level was observed. However, it was nonsignificant.
Conclusion:
By observing the abnormality on the RBC count and SGOT enzyme level in the 50 ppm group, we concluded a slight toxicity effect for AuNPs and the threat potential of their use in human.
This document lists 24 publications by B. Easwaramoorthy and E. Balasubramaniam from 1992-2005 and 23 publications by B. Easwaramoorthy from 2005-present. The publications cover a wide range of topics including neurobehavioral toxicity of insecticides, photoelectrochemical properties of porphyrin systems, electroluminescent materials, and radiotracers for positron emission tomography imaging. Many of the recent publications focus on development and evaluation of novel radiotracers to image nicotinic acetylcholine and other neuroreceptors.
Yuanchao Ye is a postdoctoral fellow in the Department of Pharmacology at the University of Iowa. He received his Ph.D. in Pharmacology from Shenyang Pharmaceutical University in China in 2012. His research focuses on cell biology, molecular biology, and animal experiments related to cancer, apoptosis, autophagy, and cell signaling pathways. He has over 15 peer-reviewed publications and has served as a reviewer for several academic journals. His skills include cell culture, molecular techniques, animal handling, and data analysis software.
This curriculum vitae summarizes the qualifications and experience of B. Annadurai. He holds a M.Sc. in Botany, D.H.Ed. in Education, and Ph.D. in Biochemistry. He has held various professor positions teaching biotechnology in India and Ethiopia. His research has focused on plant molecular biology, biochemistry, and biotechnology. He has over 30 years of research experience and has published extensively in his areas of expertise.
In silico characterization of industrial important cellulases using computati...Alexander Decker
This document summarizes a study that used computational tools to characterize cellulase enzymes from different Pseudomonas species. The researchers analyzed physicochemical properties, secondary structure, multiple sequence alignments, phylogenetic relationships, and 3D structures of the cellulases. Key findings include:
- Molecular weights ranged from 40,927 to 100,058 Daltons and isoelectric points were acidic.
- Secondary structure prediction showed random coils dominated over other structures.
- Phylogenetic analysis using neighbor joining showed relationships between the cellulases.
- Homology modeling was used to predict 3D structures, providing insight into function.
2014 increasing survival study of kidney hek-293 tOscar Moreno
This document summarizes a study that examined the effects of magnetic field stimulation on the growth of Human Embryonic Kidney 293 cells (HEK-293T cells). The cells were exposed to magnetic field vortices at frequencies between 100-2500 Hz and field intensities of 1.13-4.13 mT. A nanofluid containing gadolinium was also added to the cells. Flow cytometry analysis after 72 hours found that cell survival increased by 12.89% in the exposed cells compared to the unexposed control cells. The results suggest that magnetic field stimulation may activate cellular mechanisms that promote increased cell survival. Further experiments are still needed to fully understand the magnetic effects on cell growth.
Characterizations and optimization study on influence of different parameters...eSAT Journals
Abstract
A new technological approach was used for the production of silver nanoparticles using metal acquiring microorganism as a
means of fabrication and making of superior biomaterials. Silver nanoparticles (AgNPs) are nanoparticles of silver which are in
the range of 1 and 100 nm in size. Extracellular synthesis of AgNPs was observed when Streptomyces species was exposed to
colloidal silver salt solution optimised at 1mM ionic strength by autoclaving method.UV-Visible spectroscopic analysis were done
to monitor the drift in change of pH, precursor concentration, Streptomyces supernatant volume and effect of temperature on the
formation of AgNPs. Influence of different factors such as pH, initial silver molar strength, water dilution, and methodology used o
synthesize nanoparticles were found to be extremely reliable for consistent stability and storage purpose. The results indicated
that temperature optimised was at 280C for storage, pH was at neutral 7 and water orchestrated as “diluent”, showed increased
Nano-particle spectra. The formation of nanoparticles was first monitored by measuring the surface plasmon resonance (SPR)
band at 410nm by UV-Visible absorption spectroscopy. The presence of elemental silver and the crystalline structure of the
AgNPs were confirmed by Thermogravimetric analysis (TGA) and Powder X-ray diffraction (PXRD).In addition, Atomic Force
Microscopy (AFM) along with FESEM imaging used for characterising nanoparticles revealed that they in spherical shape with
an average particle size of 20-70nm.Finally, FTIR investigations revealed that many efficient clusters of functional bio molecules
are playing significant role in capping and synthesis process.
Keywords: Antagonism, Silver Nanoparticles (AgNPs), 16S Ribosomal Gene, Atomic Force Microscopy (AFM).
This study examined the effects of copper nanoparticles on liver tissue and function in rats. Rats were injected with different doses of copper nanoparticles and examined after 2, 7, and 14 days. Histological analysis found changes in liver and lung tissue like thickened air sac walls and increased fibrous tissue. Biochemical analysis found elevated levels of hepatic enzymes SGOT and SGPT, indicating liver damage. The highest doses of copper nanoparticles caused the most severe effects. This suggests that copper nanoparticles can cause toxicity in liver and lung tissues.
Radiation Response of Bacteria Associated with Human Cancellous BoneIOSR Journals
Cancellous bones from twenty five live tissue donors were tested for bacterial contamination and initial bioburden ranged from 4.1×101 to 3.1×103 cfu/g (average 9.0×102 cfu/g). Forty six representative bacterial isolates were characterized on the basis of morphological, cultural and biochemical characteristics. Staphylococcus spp. was found to be predominant contaminant in tissue samples (41.30%). To assess the radiation resistance all the bacterial isolates were exposed to 1 to 10 kGy gamma radiation from 60Co gamma source. The radiation decimal reduction dose values (D10) and twelve log reduction values (12 D value) of the isolates were calculated. D10 values of the isolates were ranged from 0.59 to 1.20 kGy. Among the studied bacterial isolates, Streptococcus spp. was the most radioresistant isolates (D10 value 0.93-1.20 kGy) and three of the Streptococcus spp. survived up to 8 kGy. All the bacterial isolates were killed at 9 kGy. Twelve log reduction value (12D value) of the most resistant isolate was 14.4 kGy. These results indicate that standard radiation sterilization dose (25 kGy) is satisfactory for the sterilization of the cancellous bone allografts
Morphohistometric analysis of the effects of Coriandrum sativum on cortical a...Prof. Hesham N. Mustafa
The document summarizes a study that analyzed the effects of Coriandrum sativum (C. sativum) on lead-induced neurotoxicity in the cerebellar cortex and somatosensory cortex of rats. The study found that lead exposure increased oxidative stress in the brain and caused structural changes in the cerebellar and cortical layers. However, supplementation with C. sativum extract reduced lead levels in the blood and brain, decreased oxidative stress, and corrected the changes to layer thickness and nuclei density caused by lead exposure. The results suggest that C. sativum has protective effects against lead neurotoxicity due to its antioxidant and metal-chelating properties.
1. The document discusses the recombinant expression and purification of the two subunit proteins, IFα and IFγ, that make up hagfish slime intermediate filaments.
2. Methods are described for expressing the proteins individually and together in bacterial vectors, purifying the proteins using nickel chromatography, and forming a biopolymer membrane through dialysis and drop casting of the purified proteins.
3. Key results showed that individual expression of IFα and IFγ was successful but co-expression was not. Purification methods concentrated the proteins and dialysis formed a biopolymer membrane modeling hagfish slime threads.
1) Current in vitro drug development models do not fully replicate the complex in vivo environment, leading to drugs being withdrawn from the market after approval.
2) New tissue engineering approaches are being developed to create more physiologically relevant in vitro models using techniques like organ-on-chip, 3D bioprinting, and engineered tissues.
3) The presentation describes research developing improved 3D models of the heart and intestine to better predict drug toxicity and study disease, including models with relevant cell types, extracellular matrix interactions, tissue architecture, and barrier functions.
Evaluation of the Impact of Biofield Treatment on Physical and Thermal Proper...wilhelm mendel
In the present study, the influence of biofield treatment on physical and thermal properties of Casein Enzyme Hydrolysate (CEH) and Casein Yeast Peptone (CYP) were investigated. The control and treated samples were characterized by Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC), Thermo Gravimetric Analysis (TGA), particle size and surface area analysis. The FTIR results revealed that biofield treatment has caused reduction of amide group (amide-I and amide-II) stretching vibration peak that is associated with strong intermolecular hydrogen bonding in treated CEH as compared to control. However, no significant changes were observed in FTIR spectrum of treated CYP. The TGA analysis of treated CEH showed a substantial improvement in thermal stability which was confirmed by increase in maximum thermal decomposition temperature (217°C) as compared to control (209°C). Similarly, the treated CYP also showed enhanced thermal stability as compared to control. DSC showed increase in melting temperature of treated CYP as compared to control. However the melting peak was absent in DSC of treated CEH which was probably due to rigid chain of the protein. The surface area of treated CEH was increased by 83% as compared to control. However, a decrease (7.3%) in surface area was observed in treated CYP. The particle size analysis of treated CEH showed a significant increase in average particle size (d50) and d99 value (maximum particle size below which 99% of particles are present) as compared to control sample. Similarly, the treated CYP also showed a substantial increase in d50 and d99 values which was probably due to the agglomeration of the particles which led to formation of bigger microparticles. The result showed that the biofield treated CEH and CYP could be used as a matrix for pharmaceutical applications.
Ameliorative potentials of a combination of fenugreek and alpha-tocopherol on...Prof. Hesham N. Mustafa
Background: The current study aimed to elucidate the protective role of combined fenugreek and a-tocopherol against cadmium induced histopathological
changes in the testes.
Materials and methods: Thirty adult male albino rats divided into three equal
groups 10 rats each. Group I is the control group. Group II received 5 mg/kg/day
cadmium chloride. Group III received 5 mg/kg/day cadmium chloride and 150
mg/kg/day fenugreek and 100 mg/kg/day of a-tocopherol. The treatment of all
groups was done by oral gavage for 60 consecutive days. The testes were removed
and subjected to histopathological and ultrastructure study.
Results: Rats exposed to cadmium showed severe histopathological changes in
the testicular spermatogenic series, many vacuoles and multinucleated giant cells.
Treatment with fenugreek and a-tocopherol partially improved the morphological
changes, reduced tissue damage and rebuilt of the spermatogonia layer.
Conclusions: Fenugreek and a-tocopherol might represent a promising medicinal
combination to ameliorate the toxic effects of cadmium exposure. (Folia Morphol
2015; 74, 3: 325–334)
Key words: cadmium chloride, fenugreek, a-tocopherol, seminiferous
epithelium, ultrastructure
Evaluation of the safety of conventional lighting replacement by artificial d...Prof. Hesham N. Mustafa
Background
Short morning exposure to high illuminance visible electromagnetic radiations termed as artificial daylight is beneficial for the mental health of people living in geographical areas with important seasonal changes in daylight illuminance. However, the commercial success of high illuminance light sources has raised the question of the safety of long hour exposure.
Methods
We have investigated the effect of the replacement of natural daylight by artificial daylight in Swiss mice raised under natural lighting conditions. Mice were monitored for neurotoxicity and general health changes. They were submitted to a battery of conventional tests for mood, motor and cognitive functions’ assessment on exposure day (ED) 14 and ED20. Following sacrifice on ED21 due to marked signs of neurotoxicity, the expression of markers of inflammation and apoptosis was assessed in the entorhinal cortex and neurons were estimated in the hippocampal formation.
Results
Signs of severe cognitive and motor impairments, mood disorders, and hepatotoxicity were observed in animals exposed to artificial daylight on ED20, unlike on ED14 and unlike groups exposed to natural daylight or conventional lighting. Activated microglia and astrocytes were observed in the entorhinal cortex, as well as dead and dying neurons. Neuronal counts revealed massive neuronal loss in the hippocampal formation.
Conclusions
These results suggest that long hour exposure to high illuminance visible electromagnetic radiations induced severe alterations in brain function and general health in mice partly mediated by damages to the neocortex-entorhinal cortex-hippocampus axis. These findings raise caution over long hour use of high illuminance artificial light.
1. The study characterized Echinococcus granulosus genotypes from cyst samples collected from buffalo, sheep, and humans in Bangladesh using PCR of mitochondrial genes.
2. Two genotypes were identified: the common sheep strain G1 and the buffalo strain G3. Nine of 15 buffalo samples and 6 of 9 sheep samples tested positive for G1 using 12S rRNA, while 3 buffalo tested positive for G3 using COX1.
3. Sequence analysis revealed close identity between Bangladeshi isolates and reference sequences for G1 and G3 from other countries. This is the first molecular characterization of Echinococcus spp. in Bangladesh.
Genotoxicity Evaluation of Polystyrene Membrane with Collagen and Norbixin by...inventionjournals
The biocompatible membranes are widely applied in the medical field in order to stimulate tissue repair. The biological principle of this type of treatment is the repair and guided regeneration. In the literature, there are few reports of studies evaluating the effects and biological properties of norbixin in animal tissues. Thus, the present study was to evaluate the effect of polystyrene membrane with collagen and norbixin, through the micronucleus test and comet assay in rats, as part of the recommended test battery to evaluate the mutagenic potential. The research project was approved by CEP / FACID Protocol 069/2014. For this study, 15 rats were divided into 3 groups were used: A - the membrane was introduced into the peritoneum of the animals through a laparotomy; B - received cyclophosphamide at a dose of 50mg / kg intraperitoneally; C - were performed only one laparotomy. A peripheral blood sample was collected from the animals for conducting Comet assay and 72 hours after the start of the experiment were euthanized. It was collected bone marrow material of each rat to perform the micronucleus test. In conclusion, through the tests, the membrane is not genotoxic
This document summarizes a study that investigated the larvicidal effects of Aegle marmelos (bael tree) leaf extracts on Aedes aegypti mosquitoes. Specifically, it assessed the efficacy of methanol extracts from A. marmelos leaves in killing A. aegypti larvae (at the third instar stage) and altering their midgut proteins. The study found that the leaf extract achieved 50% larval mortality (LC50) at a concentration of 49 ppm. Proteomic analysis of larval midguts revealed changes in protein expression levels after exposure to the extract, suggesting its bioactive compounds can disrupt the midgut. The aim is to identify specific inhibitor proteins in the midg
Evaluation of In-vitro neuroprotective effect of Ethanolic extract of Canariu...AI Publications
The ethanolic extract of canarium solomonense leaves (ecsl) was studied for its neuroprotective activity. The neuroprotective activity of ECSL was found to have a significant impact on neuronal cell death triggered by hydrogen peroxide (MTT assay) in human SH-SY5Y neuroblastoma cells. Scopolamine, a muscarinic receptor blocker, is frequently used to induce cognitive impairment in laboratory animals. Injections of scopolamine influence multiple cognitive functions, including motor function, short-term memory, and attention. Using the Morris water maze, the Y maze, and the passive avoidance paradigm, memory enhancing activity in scopolamine-induced amnesic rats was evaluated. Using the Morris water maze, the Y maze, and the passive avoidance paradigm, ECSL was found to have a substantial effect on the memory of scopolamine- induced amnesic rats. Our experimental data indicated that ECSL can reverse scopolamine induced amnesia and assist with memory issues.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Genotoxicity of Goji Berry (Lyciumbarbarum) In Vivo Mammalian Cellsinventionjournals
Lyciumbarbarum (Gojji berry) belongs to family Salonaceae which is found in China and Himalayan. This herb is used to prevent various diseases and in medical treatments as an alternative medicine being widely used for its antioxidant and revitalizing potential effects. In recent years, Gojji has become increasingly popular in Europe and North America as a "superfruit" and dietary supplement. The belief that herbal products do not bring any risk to health, is part of popular culture. However the term "natural" assigned to many products cannot assure no health risk. The aim of this study was to evaluate the possible genotoxic effects of aqueous extract of Lyciumbarbarum (Gojji berry) by micronucleus test and comet assay. Thirty Rattus norvegicus were divided into three equal groups: 1) experimental group, submitted to Gojji berry (200mg/kg orally); 2) positive control group (cyclophosphamide), and; 3) negative control group (distilled water). Micronucleus Tests were done by smear method of bone marrow cells performed after 48h for acute, and 72h for chronic exposure. The comet assay was performed on peripheral blood taken from the tail of each animal 4h, and 24h after intervention. Cytotoxicity was assessed by observing the DNA damage measuring the percentage of DNA in the tail (% DNA- measurement of the proportion of the total DNA present in the tail) and the tail moment (TM-tail length times the percentage of DNA in the tail), calculated by 100 nucleoids per animal and the presence of micronuclei in 2,000 polychromatic erythrocytes per animal. Analysis of variance (ANOVA) followed by Tukey test at 5% significance was used comparing the results. The data showed no significant difference in the frequency of DNA damage and the number of micronuclei between the experimental group and the negative control group. The results also suggest that the aqueous extract of Lyciumbarbarum (Gojji berry) at the dose of 200 mg/kg showed no genotoxic effect, which could, to a certain point, justifies its use.
This document lists 24 publications by B. Easwaramoorthy and E. Balasubramaniam from 1992-2005 and 23 publications by B. Easwaramoorthy from 2005-present. The publications cover a wide range of topics including neurobehavioral toxicity of insecticides, photoelectrochemical properties of porphyrin systems, electroluminescent materials, and radiotracers for positron emission tomography imaging. Many of the recent publications focus on development and evaluation of novel radiotracers to image nicotinic acetylcholine and other neuroreceptors.
Yuanchao Ye is a postdoctoral fellow in the Department of Pharmacology at the University of Iowa. He received his Ph.D. in Pharmacology from Shenyang Pharmaceutical University in China in 2012. His research focuses on cell biology, molecular biology, and animal experiments related to cancer, apoptosis, autophagy, and cell signaling pathways. He has over 15 peer-reviewed publications and has served as a reviewer for several academic journals. His skills include cell culture, molecular techniques, animal handling, and data analysis software.
This curriculum vitae summarizes the qualifications and experience of B. Annadurai. He holds a M.Sc. in Botany, D.H.Ed. in Education, and Ph.D. in Biochemistry. He has held various professor positions teaching biotechnology in India and Ethiopia. His research has focused on plant molecular biology, biochemistry, and biotechnology. He has over 30 years of research experience and has published extensively in his areas of expertise.
In silico characterization of industrial important cellulases using computati...Alexander Decker
This document summarizes a study that used computational tools to characterize cellulase enzymes from different Pseudomonas species. The researchers analyzed physicochemical properties, secondary structure, multiple sequence alignments, phylogenetic relationships, and 3D structures of the cellulases. Key findings include:
- Molecular weights ranged from 40,927 to 100,058 Daltons and isoelectric points were acidic.
- Secondary structure prediction showed random coils dominated over other structures.
- Phylogenetic analysis using neighbor joining showed relationships between the cellulases.
- Homology modeling was used to predict 3D structures, providing insight into function.
2014 increasing survival study of kidney hek-293 tOscar Moreno
This document summarizes a study that examined the effects of magnetic field stimulation on the growth of Human Embryonic Kidney 293 cells (HEK-293T cells). The cells were exposed to magnetic field vortices at frequencies between 100-2500 Hz and field intensities of 1.13-4.13 mT. A nanofluid containing gadolinium was also added to the cells. Flow cytometry analysis after 72 hours found that cell survival increased by 12.89% in the exposed cells compared to the unexposed control cells. The results suggest that magnetic field stimulation may activate cellular mechanisms that promote increased cell survival. Further experiments are still needed to fully understand the magnetic effects on cell growth.
Characterizations and optimization study on influence of different parameters...eSAT Journals
Abstract
A new technological approach was used for the production of silver nanoparticles using metal acquiring microorganism as a
means of fabrication and making of superior biomaterials. Silver nanoparticles (AgNPs) are nanoparticles of silver which are in
the range of 1 and 100 nm in size. Extracellular synthesis of AgNPs was observed when Streptomyces species was exposed to
colloidal silver salt solution optimised at 1mM ionic strength by autoclaving method.UV-Visible spectroscopic analysis were done
to monitor the drift in change of pH, precursor concentration, Streptomyces supernatant volume and effect of temperature on the
formation of AgNPs. Influence of different factors such as pH, initial silver molar strength, water dilution, and methodology used o
synthesize nanoparticles were found to be extremely reliable for consistent stability and storage purpose. The results indicated
that temperature optimised was at 280C for storage, pH was at neutral 7 and water orchestrated as “diluent”, showed increased
Nano-particle spectra. The formation of nanoparticles was first monitored by measuring the surface plasmon resonance (SPR)
band at 410nm by UV-Visible absorption spectroscopy. The presence of elemental silver and the crystalline structure of the
AgNPs were confirmed by Thermogravimetric analysis (TGA) and Powder X-ray diffraction (PXRD).In addition, Atomic Force
Microscopy (AFM) along with FESEM imaging used for characterising nanoparticles revealed that they in spherical shape with
an average particle size of 20-70nm.Finally, FTIR investigations revealed that many efficient clusters of functional bio molecules
are playing significant role in capping and synthesis process.
Keywords: Antagonism, Silver Nanoparticles (AgNPs), 16S Ribosomal Gene, Atomic Force Microscopy (AFM).
This study examined the effects of copper nanoparticles on liver tissue and function in rats. Rats were injected with different doses of copper nanoparticles and examined after 2, 7, and 14 days. Histological analysis found changes in liver and lung tissue like thickened air sac walls and increased fibrous tissue. Biochemical analysis found elevated levels of hepatic enzymes SGOT and SGPT, indicating liver damage. The highest doses of copper nanoparticles caused the most severe effects. This suggests that copper nanoparticles can cause toxicity in liver and lung tissues.
Radiation Response of Bacteria Associated with Human Cancellous BoneIOSR Journals
Cancellous bones from twenty five live tissue donors were tested for bacterial contamination and initial bioburden ranged from 4.1×101 to 3.1×103 cfu/g (average 9.0×102 cfu/g). Forty six representative bacterial isolates were characterized on the basis of morphological, cultural and biochemical characteristics. Staphylococcus spp. was found to be predominant contaminant in tissue samples (41.30%). To assess the radiation resistance all the bacterial isolates were exposed to 1 to 10 kGy gamma radiation from 60Co gamma source. The radiation decimal reduction dose values (D10) and twelve log reduction values (12 D value) of the isolates were calculated. D10 values of the isolates were ranged from 0.59 to 1.20 kGy. Among the studied bacterial isolates, Streptococcus spp. was the most radioresistant isolates (D10 value 0.93-1.20 kGy) and three of the Streptococcus spp. survived up to 8 kGy. All the bacterial isolates were killed at 9 kGy. Twelve log reduction value (12D value) of the most resistant isolate was 14.4 kGy. These results indicate that standard radiation sterilization dose (25 kGy) is satisfactory for the sterilization of the cancellous bone allografts
Morphohistometric analysis of the effects of Coriandrum sativum on cortical a...Prof. Hesham N. Mustafa
The document summarizes a study that analyzed the effects of Coriandrum sativum (C. sativum) on lead-induced neurotoxicity in the cerebellar cortex and somatosensory cortex of rats. The study found that lead exposure increased oxidative stress in the brain and caused structural changes in the cerebellar and cortical layers. However, supplementation with C. sativum extract reduced lead levels in the blood and brain, decreased oxidative stress, and corrected the changes to layer thickness and nuclei density caused by lead exposure. The results suggest that C. sativum has protective effects against lead neurotoxicity due to its antioxidant and metal-chelating properties.
1. The document discusses the recombinant expression and purification of the two subunit proteins, IFα and IFγ, that make up hagfish slime intermediate filaments.
2. Methods are described for expressing the proteins individually and together in bacterial vectors, purifying the proteins using nickel chromatography, and forming a biopolymer membrane through dialysis and drop casting of the purified proteins.
3. Key results showed that individual expression of IFα and IFγ was successful but co-expression was not. Purification methods concentrated the proteins and dialysis formed a biopolymer membrane modeling hagfish slime threads.
1) Current in vitro drug development models do not fully replicate the complex in vivo environment, leading to drugs being withdrawn from the market after approval.
2) New tissue engineering approaches are being developed to create more physiologically relevant in vitro models using techniques like organ-on-chip, 3D bioprinting, and engineered tissues.
3) The presentation describes research developing improved 3D models of the heart and intestine to better predict drug toxicity and study disease, including models with relevant cell types, extracellular matrix interactions, tissue architecture, and barrier functions.
Evaluation of the Impact of Biofield Treatment on Physical and Thermal Proper...wilhelm mendel
In the present study, the influence of biofield treatment on physical and thermal properties of Casein Enzyme Hydrolysate (CEH) and Casein Yeast Peptone (CYP) were investigated. The control and treated samples were characterized by Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC), Thermo Gravimetric Analysis (TGA), particle size and surface area analysis. The FTIR results revealed that biofield treatment has caused reduction of amide group (amide-I and amide-II) stretching vibration peak that is associated with strong intermolecular hydrogen bonding in treated CEH as compared to control. However, no significant changes were observed in FTIR spectrum of treated CYP. The TGA analysis of treated CEH showed a substantial improvement in thermal stability which was confirmed by increase in maximum thermal decomposition temperature (217°C) as compared to control (209°C). Similarly, the treated CYP also showed enhanced thermal stability as compared to control. DSC showed increase in melting temperature of treated CYP as compared to control. However the melting peak was absent in DSC of treated CEH which was probably due to rigid chain of the protein. The surface area of treated CEH was increased by 83% as compared to control. However, a decrease (7.3%) in surface area was observed in treated CYP. The particle size analysis of treated CEH showed a significant increase in average particle size (d50) and d99 value (maximum particle size below which 99% of particles are present) as compared to control sample. Similarly, the treated CYP also showed a substantial increase in d50 and d99 values which was probably due to the agglomeration of the particles which led to formation of bigger microparticles. The result showed that the biofield treated CEH and CYP could be used as a matrix for pharmaceutical applications.
Ameliorative potentials of a combination of fenugreek and alpha-tocopherol on...Prof. Hesham N. Mustafa
Background: The current study aimed to elucidate the protective role of combined fenugreek and a-tocopherol against cadmium induced histopathological
changes in the testes.
Materials and methods: Thirty adult male albino rats divided into three equal
groups 10 rats each. Group I is the control group. Group II received 5 mg/kg/day
cadmium chloride. Group III received 5 mg/kg/day cadmium chloride and 150
mg/kg/day fenugreek and 100 mg/kg/day of a-tocopherol. The treatment of all
groups was done by oral gavage for 60 consecutive days. The testes were removed
and subjected to histopathological and ultrastructure study.
Results: Rats exposed to cadmium showed severe histopathological changes in
the testicular spermatogenic series, many vacuoles and multinucleated giant cells.
Treatment with fenugreek and a-tocopherol partially improved the morphological
changes, reduced tissue damage and rebuilt of the spermatogonia layer.
Conclusions: Fenugreek and a-tocopherol might represent a promising medicinal
combination to ameliorate the toxic effects of cadmium exposure. (Folia Morphol
2015; 74, 3: 325–334)
Key words: cadmium chloride, fenugreek, a-tocopherol, seminiferous
epithelium, ultrastructure
Evaluation of the safety of conventional lighting replacement by artificial d...Prof. Hesham N. Mustafa
Background
Short morning exposure to high illuminance visible electromagnetic radiations termed as artificial daylight is beneficial for the mental health of people living in geographical areas with important seasonal changes in daylight illuminance. However, the commercial success of high illuminance light sources has raised the question of the safety of long hour exposure.
Methods
We have investigated the effect of the replacement of natural daylight by artificial daylight in Swiss mice raised under natural lighting conditions. Mice were monitored for neurotoxicity and general health changes. They were submitted to a battery of conventional tests for mood, motor and cognitive functions’ assessment on exposure day (ED) 14 and ED20. Following sacrifice on ED21 due to marked signs of neurotoxicity, the expression of markers of inflammation and apoptosis was assessed in the entorhinal cortex and neurons were estimated in the hippocampal formation.
Results
Signs of severe cognitive and motor impairments, mood disorders, and hepatotoxicity were observed in animals exposed to artificial daylight on ED20, unlike on ED14 and unlike groups exposed to natural daylight or conventional lighting. Activated microglia and astrocytes were observed in the entorhinal cortex, as well as dead and dying neurons. Neuronal counts revealed massive neuronal loss in the hippocampal formation.
Conclusions
These results suggest that long hour exposure to high illuminance visible electromagnetic radiations induced severe alterations in brain function and general health in mice partly mediated by damages to the neocortex-entorhinal cortex-hippocampus axis. These findings raise caution over long hour use of high illuminance artificial light.
1. The study characterized Echinococcus granulosus genotypes from cyst samples collected from buffalo, sheep, and humans in Bangladesh using PCR of mitochondrial genes.
2. Two genotypes were identified: the common sheep strain G1 and the buffalo strain G3. Nine of 15 buffalo samples and 6 of 9 sheep samples tested positive for G1 using 12S rRNA, while 3 buffalo tested positive for G3 using COX1.
3. Sequence analysis revealed close identity between Bangladeshi isolates and reference sequences for G1 and G3 from other countries. This is the first molecular characterization of Echinococcus spp. in Bangladesh.
Genotoxicity Evaluation of Polystyrene Membrane with Collagen and Norbixin by...inventionjournals
The biocompatible membranes are widely applied in the medical field in order to stimulate tissue repair. The biological principle of this type of treatment is the repair and guided regeneration. In the literature, there are few reports of studies evaluating the effects and biological properties of norbixin in animal tissues. Thus, the present study was to evaluate the effect of polystyrene membrane with collagen and norbixin, through the micronucleus test and comet assay in rats, as part of the recommended test battery to evaluate the mutagenic potential. The research project was approved by CEP / FACID Protocol 069/2014. For this study, 15 rats were divided into 3 groups were used: A - the membrane was introduced into the peritoneum of the animals through a laparotomy; B - received cyclophosphamide at a dose of 50mg / kg intraperitoneally; C - were performed only one laparotomy. A peripheral blood sample was collected from the animals for conducting Comet assay and 72 hours after the start of the experiment were euthanized. It was collected bone marrow material of each rat to perform the micronucleus test. In conclusion, through the tests, the membrane is not genotoxic
This document summarizes a study that investigated the larvicidal effects of Aegle marmelos (bael tree) leaf extracts on Aedes aegypti mosquitoes. Specifically, it assessed the efficacy of methanol extracts from A. marmelos leaves in killing A. aegypti larvae (at the third instar stage) and altering their midgut proteins. The study found that the leaf extract achieved 50% larval mortality (LC50) at a concentration of 49 ppm. Proteomic analysis of larval midguts revealed changes in protein expression levels after exposure to the extract, suggesting its bioactive compounds can disrupt the midgut. The aim is to identify specific inhibitor proteins in the midg
Evaluation of In-vitro neuroprotective effect of Ethanolic extract of Canariu...AI Publications
The ethanolic extract of canarium solomonense leaves (ecsl) was studied for its neuroprotective activity. The neuroprotective activity of ECSL was found to have a significant impact on neuronal cell death triggered by hydrogen peroxide (MTT assay) in human SH-SY5Y neuroblastoma cells. Scopolamine, a muscarinic receptor blocker, is frequently used to induce cognitive impairment in laboratory animals. Injections of scopolamine influence multiple cognitive functions, including motor function, short-term memory, and attention. Using the Morris water maze, the Y maze, and the passive avoidance paradigm, memory enhancing activity in scopolamine-induced amnesic rats was evaluated. Using the Morris water maze, the Y maze, and the passive avoidance paradigm, ECSL was found to have a substantial effect on the memory of scopolamine- induced amnesic rats. Our experimental data indicated that ECSL can reverse scopolamine induced amnesia and assist with memory issues.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Genotoxicity of Goji Berry (Lyciumbarbarum) In Vivo Mammalian Cellsinventionjournals
Lyciumbarbarum (Gojji berry) belongs to family Salonaceae which is found in China and Himalayan. This herb is used to prevent various diseases and in medical treatments as an alternative medicine being widely used for its antioxidant and revitalizing potential effects. In recent years, Gojji has become increasingly popular in Europe and North America as a "superfruit" and dietary supplement. The belief that herbal products do not bring any risk to health, is part of popular culture. However the term "natural" assigned to many products cannot assure no health risk. The aim of this study was to evaluate the possible genotoxic effects of aqueous extract of Lyciumbarbarum (Gojji berry) by micronucleus test and comet assay. Thirty Rattus norvegicus were divided into three equal groups: 1) experimental group, submitted to Gojji berry (200mg/kg orally); 2) positive control group (cyclophosphamide), and; 3) negative control group (distilled water). Micronucleus Tests were done by smear method of bone marrow cells performed after 48h for acute, and 72h for chronic exposure. The comet assay was performed on peripheral blood taken from the tail of each animal 4h, and 24h after intervention. Cytotoxicity was assessed by observing the DNA damage measuring the percentage of DNA in the tail (% DNA- measurement of the proportion of the total DNA present in the tail) and the tail moment (TM-tail length times the percentage of DNA in the tail), calculated by 100 nucleoids per animal and the presence of micronuclei in 2,000 polychromatic erythrocytes per animal. Analysis of variance (ANOVA) followed by Tukey test at 5% significance was used comparing the results. The data showed no significant difference in the frequency of DNA damage and the number of micronuclei between the experimental group and the negative control group. The results also suggest that the aqueous extract of Lyciumbarbarum (Gojji berry) at the dose of 200 mg/kg showed no genotoxic effect, which could, to a certain point, justifies its use.
Genotoxicity of Goji Berry (Lyciumbarbarum) In Vivo Mammalian Cellsinventionjournals
Lyciumbarbarum (Gojji berry) belongs to family Salonaceae which is found in China and Himalayan. This herb is used to prevent various diseases and in medical treatments as an alternative medicine being widely used for its antioxidant and revitalizing potential effects. In recent years, Gojji has become increasingly popular in Europe and North America as a "superfruit" and dietary supplement. The belief that herbal products do not bring any risk to health, is part of popular culture. However the term "natural" assigned to many products cannot assure no health risk. The aim of this study was to evaluate the possible genotoxic effects of aqueous extract of Lyciumbarbarum (Gojji berry) by micronucleus test and comet assay. Thirty Rattus norvegicus were divided into three equal groups: 1) experimental group, submitted to Gojji berry (200mg/kg orally); 2) positive control group (cyclophosphamide), and; 3) negative control group (distilled water). Micronucleus Tests were done by smear method of bone marrow cells performed after 48h for acute, and 72h for chronic exposure. The comet assay was performed on peripheral blood taken from the tail of each animal 4h, and 24h after intervention. Cytotoxicity was assessed by observing the DNA damage measuring the percentage of DNA in the tail (% DNA- measurement of the proportion of the total DNA present in the tail) and the tail moment (TM-tail length times the percentage of DNA in the tail), calculated by 100 nucleoids per animal and the presence of micronuclei in 2,000 polychromatic erythrocytes per animal. Analysis of variance (ANOVA) followed by Tukey test at 5% significance was used comparing the results. The data showed no significant difference in the frequency of DNA damage and the number of micronuclei between the experimental group and the negative control group. The results also suggest that the aqueous extract of Lyciumbarbarum (Gojji berry) at the dose of 200 mg/kg showed no genotoxic effect, which could, to a certain point, justifies its use.
Genotoxicity of Goji Berry (Lyciumbarbarum) In Vivo Mammalian Cellsinventionjournals
This document summarizes a study that evaluated the genotoxic effects of an aqueous extract of Goji berry (Lyciumbarbarum) using micronucleus and comet assays in rats. Rats were divided into three groups: an experimental group that received 200 mg/kg of the Goji berry extract orally, a positive control group that received cyclophosphamide, and a negative control group. The comet assay showed no significant increase in DNA damage in the experimental group compared to the negative control group at 4 or 24 hours. The micronucleus test also found no significant difference in micronuclei between the experimental and negative control groups for acute or chronic exposure. The results suggest that the Goji berry
Protein was extracted from muscles of Channa striatus and attempts were
made to evaluate in vitro antibacterial activity against clinical bacterial isolates. The
higher concentration of protein (100μg/ml) extracts exhibited a pronounced activity
against Pseudomonas aeruginosa (21 mm), Proteus vulgaris (19 mm), Citrobacter sp
(19 mm), Klebsiella pneumoniae (18 mm), Micrococcus sp (17 mm), Bacillus subtilis (16
mm), Staphylococcus aureus (15 mm), E. coli (14 mm) and Serratia marcescens (5
mm). The minimum inhibitory concentration and minimum bactericidal concentration
were found to be 20-40 μg/ml and 80-100 μg/ml respectively for the extracts of
Channa striatus protein against test organisms. This study confirms that C. striatus fish
protein extracts possess antibacterial activity against a wide range of microbes and
justified that it could be used in the traditional medicine as a remedy for the
treatment of bacterial diseases.
A cost effective scheme developed for studying human malaria caused by plasmo...Alexander Decker
This document summarizes a study that developed a cost-effective mouse model for studying human malaria caused by Plasmodium falciparum. Key points:
- Researchers cultured P. falciparum from infected human blood samples in vitro. They then injected cultured parasites into mice that were immunosuppressed using aspirin and doxycycline and "humanized" by injecting human blood, creating a non-genetically manipulated mouse model.
- All 20 mice inoculated with cultured parasites developed blood parasitemia, indicating the model could induce and study human malaria infection.
- Infected mice were then treated with the antimalarial drug quinine or a placebo. Quinine significantly suppressed blood
The Invention of the Mid-Infrared Generating Atomizer and its Human and Veter...semualkaira
In the current scenario, there
are different therapies for different diseases of human and animals. The existed therapies are associated with hurdles like drug
resistance, less sensitivity, side effects, uneconomical, etc. However, none of the therapies provide multi-disease management on
a molecular basis. Every disease originates due to inter and/or intra-molecular (cell/ tissue) changes, which changes their respective chemical bonds. Therapies focusing to rectify the molecular
changes (molecular medicine) are growing slowly, which could
act as a single remedy, but needs extensive multi-faculty research.
The Invention of the Mid-Infrared Generating Atomizer and its Human and Veter...semualkaira
In the current scenario, there
are different therapies for different diseases of human and animals. The existed therapies are associated with hurdles like drug
resistance, less sensitivity, side effects, uneconomical, etc. However, none of the therapies provide multi-disease management on
a molecular basis. Every disease originates due to inter and/or intra-molecular (cell/ tissue) changes, which changes their respective chemical bonds. Therapies focusing to rectify the molecular
changes (molecular medicine) are growing slowly, which could
act as a single remedy, but needs extensive multi-faculty research.
The Invention of the Mid-Infrared Generating Atomizer and its Human and Veter...semualkaira
In the current scenario, there
are different therapies for different diseases of human and animals. The existed therapies are associated with hurdles like drug
resistance, less sensitivity, side effects, uneconomical, etc. However, none of the therapies provide multi-disease management on
a molecular basis. Every disease originates due to inter and/or intra-molecular (cell/ tissue) changes, which changes their respective chemical bonds. Therapies focusing to rectify the molecular
changes (molecular medicine) are growing slowly, which could
act as a single remedy, but needs extensive multi-faculty research.
Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimu...ijtsrd
In this study, clinical isolate of Staphylococcus aureus was subjected to susceptibility tests against commercially available antibiotics and Ocimum gratissimum scent leaf leaf extracts. disc diffusion nmethod was adopted for the antibiotics test while agar well diffusion technique was employed for the antimicrobial screening of the leaf extracts. The result shows that the organism was sensitive to Gentamycin, Zinnacef, Ciprofloxacin and Streptomycin representing 40 of the antibiotics while showing outright resistance to Ampiclox, Amoxicillin and Erythromycin 30 and intermediate results for the rest. Also, the cold water extract of O. gratissimum did not show any activity against the bacterium. However, both hot water extract and ethanol extract of the plant had varying degree of activity against the organism with ethanol extract recording a zone of inhibition of 25.33 mm compared with 32.00 mm recorded for Gentamycin. Furthermore, the minimum inhibitory concentration recorded ofr the extract against the organism were 10 mg ml and 2.5 mg ml respectively for hot water and ethanol extracts. These results indicate that ethanol extract of O. gratissimum leaf is very effective against Staphylococcus aureus and could be used as potential source of natural product for the treatment of infections caused by the organism especially the antibiotic resistant strains. Komolafe T. O. | Ogunyankin O. G "Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimum Extract" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46333.pdf Paper URL : https://www.ijtsrd.com/other-scientific-research-area/other/46333/antibiogram-of-staphylococcus-aureus-and-its-sensitivity-to-ocimum-gratissimum-extract/komolafe-t-o
This study investigated the protective effects of Salacia oblanga and quercetin on cyclophosphamide-induced chromosome aberrations in rat bone marrow cells. Rats were treated with Salacia oblanga or quercetin for 15 days, then given cyclophosphamide on days 14 and 15. Cyclophosphamide is known to induce chromosome aberrations and oxidative stress. The study found that quercetin completely prevented cyclophosphamide-induced chromosome aberrations, while Salacia oblanga partially prevented them. Both treatments decreased oxidative stress caused by cyclophosphamide. The results suggest that Salacia oblanga and quercetin can protect against the genotoxic and oxidative effects of cyclophosph
Genotoxicity Evaluation of Polystyrene Membrane with Collagen and Norbixin by...inventionjournals
The biocompatible membranes are widely applied in the medical field in order to stimulate tissue repair. The biological principle of this type of treatment is the repair and guided regeneration. In the literature, there are few reports of studies evaluating the effects and biological properties of norbixin in animal tissues. Thus, the present study was to evaluate the effect of polystyrene membrane with collagen and norbixin, through the micronucleus test and comet assay in rats, as part of the recommended test battery to evaluate the mutagenic potential. The research project was approved by CEP / FACID Protocol 069/2014. For this study, 15 rats were divided into 3 groups were used: A - the membrane was introduced into the peritoneum of the animals through a laparotomy; B - received cyclophosphamide at a dose of 50mg / kg intraperitoneally; C - were performed only one laparotomy. A peripheral blood sample was collected from the animals for conducting Comet assay and 72 hours after the start of the experiment were euthanized. It was collected bone marrow material of each rat to perform the micronucleus test. In conclusion, through the tests, the membrane is not genotoxic.
Genotoxicity Evaluation of Polystyrene Membrane with Collagen and Norbixin by...inventionjournals
The biocompatible membranes are widely applied in the medical field in order to stimulate tissue repair. The biological principle of this type of treatment is the repair and guided regeneration. In the literature, there are few reports of studies evaluating the effects and biological properties of norbixin in animal tissues. Thus, the present study was to evaluate the effect of polystyrene membrane with collagen and norbixin, through the micronucleus test and comet assay in rats, as part of the recommended test battery to evaluate the mutagenic potential. The research project was approved by CEP / FACID Protocol 069/2014. For this study, 15 rats were divided into 3 groups were used: A - the membrane was introduced into the peritoneum of the animals through a laparotomy; B - received cyclophosphamide at a dose of 50mg / kg intraperitoneally; C - were performed only one laparotomy. A peripheral blood sample was collected from the animals for conducting Comet assay and 72 hours after the start of the experiment were euthanized. It was collected bone marrow material of each rat to perform the micronucleus test. In conclusion, through the tests, the membrane is not genotoxic
Effects of Acalypha torta (Muell) Leaf Extract on Histological Indices of the...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
IRJET- The Antibacterial Effect of Dragonfly Larvae Diplocodes Trivialis ...IRJET Journal
The document discusses an experiment that tested the antibacterial effects of hemolymph (blood) from dragonfly larvae (Diplocodes trivialis) on three foodborne pathogens: Salmonella typhimurium, Escherichia coli, and Campylobacter jejuni. Hemolymph was collected from dragonfly larvae and tested against bacterial cultures using a plate growth-inhibition assay. The results showed that the hemolymph had antibacterial effects and inhibited the growth of S. typhimurium and E. coli. This suggests that compounds in the dragonfly hemolymph may serve as potential alternatives to conventional antibiotics. It was the first study to investigate the antibacterial properties of D. trivialis hemoly
This study investigated the effects of parietin, an anthraquinone compound isolated from Rheum ribes L, on an in vitro wound model using human dermal fibroblast cells. Parietin was isolated from Rheum ribes L and its antioxidant properties were determined using the DPPH method. An in vitro wound model was created using human dermal fibroblast cells, and different concentrations of parietin and zinc were added to test their effects on cell proliferation and viability. Parietin showed antioxidant activity and significantly increased cell viability and proliferation at concentrations of 5 to 10 μM, similar to the effects of 50 μM zinc. The results suggest that parietin may promote wound healing at low doses by inducing dermal fibro
Non-human primates in research and safety testingGreenFacts
Every year, more than 100 000 monkeys and apes are used for biomedical research around the world. Their genetic similarities to humans make them particularly suitable candidates for testing the safety of new drugs and for studying infectious diseases or the brain. But those very similarities to humans also raise specific ethical questions about their use for scientific experiments.
Are there alternatives to the use of non-human primates in research and testing? Would it be feasible to stop using them altogether?
The document discusses a primary screening of actinomycetes isolated from arid zones in Kazakhstan for their potential antitumor activity. 157 actinomycete strains were tested against Staphylococcus aureus 209P and its mutants UF-2 and UF-3 using an agar block technique. 36 strains from sandy soils and 6 from plant rhizospheres showed activity against S. aureus 209P. 22 strains from takyrs and takyr-like soils had at least twice as much activity against the mutants compared to the stock strain, indicating potential to produce antitumor antibiotics. In total, it was estimated that 24.2% of the actinomycete strains may be potential producers of antit
Similar to POTENTIAL OF CURCUMIN IN PYRAMID CELL NECROSIS OF MICE (MUS MUSCULUS) DUE TO METHYLMERCURY EXPOSURE (20)
Submission Deadline: 30th September 2022
Acceptance Notification: Within Three Days’ time period
Online Publication: Within 24 Hrs. time Period
Expected Date of Dispatch of Printed Journal: 5th October 2022
MODELING AND ANALYSIS OF SURFACE ROUGHNESS AND WHITE LATER THICKNESS IN WIRE-...IAEME Publication
White layer thickness (WLT) formed and surface roughness in wire electric discharge turning (WEDT) of tungsten carbide composite has been made to model through response surface methodology (RSM). A Taguchi’s standard Design of experiments involving five input variables with three levels has been employed to establish a mathematical model between input parameters and responses. Percentage of cobalt content, spindle speed, Pulse on-time, wire feed and pulse off-time were changed during the experimental tests based on the Taguchi’s orthogonal array L27 (3^13). Analysis of variance (ANOVA) revealed that the mathematical models obtained can adequately describe performance within the parameters of the factors considered. There was a good agreement between the experimental and predicted values in this study.
A STUDY ON THE REASONS FOR TRANSGENDER TO BECOME ENTREPRENEURSIAEME Publication
The study explores the reasons for a transgender to become entrepreneurs. In this study transgender entrepreneur was taken as independent variable and reasons to become as dependent variable. Data were collected through a structured questionnaire containing a five point Likert Scale. The study examined the data of 30 transgender entrepreneurs in Salem Municipal Corporation of Tamil Nadu State, India. Simple Random sampling technique was used. Garrett Ranking Technique (Percentile Position, Mean Scores) was used as the analysis for the present study to identify the top 13 stimulus factors for establishment of trans entrepreneurial venture. Economic advancement of a nation is governed upon the upshot of a resolute entrepreneurial doings. The conception of entrepreneurship has stretched and materialized to the socially deflated uncharted sections of transgender community. Presently transgenders have smashed their stereotypes and are making recent headlines of achievements in various fields of our Indian society. The trans-community is gradually being observed in a new light and has been trying to achieve prospective growth in entrepreneurship. The findings of the research revealed that the optimistic changes are taking place to change affirmative societal outlook of the transgender for entrepreneurial ventureship. It also laid emphasis on other transgenders to renovate their traditional living. The paper also highlights that legislators, supervisory body should endorse an impartial canons and reforms in Tamil Nadu Transgender Welfare Board Association.
BROAD UNEXPOSED SKILLS OF TRANSGENDER ENTREPRENEURSIAEME Publication
Since ages gender difference is always a debatable theme whether caused by nature, evolution or environment. The birth of a transgender is dreadful not only for the child but also for their parents. The pain of living in the wrong physique and treated as second class victimized citizen is outrageous and fully harboured with vicious baseless negative scruples. For so long, social exclusion had perpetuated inequality and deprivation experiencing ingrained malign stigma and besieged victims of crime or violence across their life spans. They are pushed into the murky way of life with a source of eternal disgust, bereft sexual potency and perennial fear. Although they are highly visible but very little is known about them. The common public needs to comprehend the ravaged arrogance on these insensitive souls and assist in integrating them into the mainstream by offering equal opportunity, treat with humanity and respect their dignity. Entrepreneurship in the current age is endorsing the gender fairness movement. Unstable careers and economic inadequacy had inclined one of the gender variant people called Transgender to become entrepreneurs. These tiny budding entrepreneurs resulted in economic transition by means of employment, free from the clutches of stereotype jobs, raised standard of living and handful of financial empowerment. Besides all these inhibitions, they were able to witness a platform for skill set development that ignited them to enter into entrepreneurial domain. This paper epitomizes skill sets involved in trans-entrepreneurs of Thoothukudi Municipal Corporation of Tamil Nadu State and is a groundbreaking determination to sightsee various skills incorporated and the impact on entrepreneurship.
DETERMINANTS AFFECTING THE USER'S INTENTION TO USE MOBILE BANKING APPLICATIONSIAEME Publication
The banking and financial services industries are experiencing increased technology penetration. Among them, the banking industry has made technological advancements to better serve the general populace. The economy focused on transforming the banking sector's system into a cashless, paperless, and faceless one. The researcher wants to evaluate the user's intention for utilising a mobile banking application. The study also examines the variables affecting the user's behaviour intention when selecting specific applications for financial transactions. The researcher employed a well-structured questionnaire and a descriptive study methodology to gather the respondents' primary data utilising the snowball sampling technique. The study includes variables like performance expectations, effort expectations, social impact, enabling circumstances, and perceived risk. Each of the aforementioned variables has a major impact on how users utilise mobile banking applications. The outcome will assist the service provider in comprehending the user's history with mobile banking applications.
ANALYSE THE USER PREDILECTION ON GPAY AND PHONEPE FOR DIGITAL TRANSACTIONSIAEME Publication
Technology upgradation in banking sector took the economy to view that payment mode towards online transactions using mobile applications. This system enabled connectivity between banks, Merchant and user in a convenient mode. there are various applications used for online transactions such as Google pay, Paytm, freecharge, mobikiwi, oxygen, phonepe and so on and it also includes mobile banking applications. The study aimed at evaluating the predilection of the user in adopting digital transaction. The study is descriptive in nature. The researcher used random sample techniques to collect the data. The findings reveal that mobile applications differ with the quality of service rendered by Gpay and Phonepe. The researcher suggest the Phonepe application should focus on implementing the application should be user friendly interface and Gpay on motivating the users to feel the importance of request for money and modes of payments in the application.
VOICE BASED ATM FOR VISUALLY IMPAIRED USING ARDUINOIAEME Publication
The prototype of a voice-based ATM for visually impaired using Arduino is to help people who are blind. This uses RFID cards which contain users fingerprint encrypted on it and interacts with the users through voice commands. ATM operates when sensor detects the presence of one person in the cabin. After scanning the RFID card, it will ask to select the mode like –normal or blind. User can select the respective mode through voice input, if blind mode is selected the balance check or cash withdraw can be done through voice input. Normal mode procedure is same as the existing ATM.
IMPACT OF EMOTIONAL INTELLIGENCE ON HUMAN RESOURCE MANAGEMENT PRACTICES AMONG...IAEME Publication
There is increasing acceptability of emotional intelligence as a major factor in personality assessment and effective human resource management. Emotional intelligence as the ability to build capacity, empathize, co-operate, motivate and develop others cannot be divorced from both effective performance and human resource management systems. The human person is crucial in defining organizational leadership and fortunes in terms of challenges and opportunities and walking across both multinational and bilateral relationships. The growing complexity of the business world requires a great deal of self-confidence, integrity, communication, conflict and diversity management to keep the global enterprise within the paths of productivity and sustainability. Using the exploratory research design and 255 participants the result of this original study indicates strong positive correlation between emotional intelligence and effective human resource management. The paper offers suggestions on further studies between emotional intelligence and human capital development and recommends for conflict management as an integral part of effective human resource management.
VISUALISING AGING PARENTS & THEIR CLOSE CARERS LIFE JOURNEY IN AGING ECONOMYIAEME Publication
Our life journey, in general, is closely defined by the way we understand the meaning of why we coexist and deal with its challenges. As we develop the "inspiration economy", we could say that nearly all of the challenges we have faced are opportunities that help us to discover the rest of our journey. In this note paper, we explore how being faced with the opportunity of being a close carer for an aging parent with dementia brought intangible discoveries that changed our insight of the meaning of the rest of our life journey.
A STUDY ON THE IMPACT OF ORGANIZATIONAL CULTURE ON THE EFFECTIVENESS OF PERFO...IAEME Publication
The main objective of this study is to analyze the impact of aspects of Organizational Culture on the Effectiveness of the Performance Management System (PMS) in the Health Care Organization at Thanjavur. Organizational Culture and PMS play a crucial role in present-day organizations in achieving their objectives. PMS needs employees’ cooperation to achieve its intended objectives. Employees' cooperation depends upon the organization’s culture. The present study uses exploratory research to examine the relationship between the Organization's culture and the Effectiveness of the Performance Management System. The study uses a Structured Questionnaire to collect the primary data. For this study, Thirty-six non-clinical employees were selected from twelve randomly selected Health Care organizations at Thanjavur. Thirty-two fully completed questionnaires were received.
Living in 21st century in itself reminds all of us the necessity of police and its administration. As more and more we are entering into the modern society and culture, the more we require the services of the so called ‘Khaki Worthy’ men i.e., the police personnel. Whether we talk of Indian police or the other nation’s police, they all have the same recognition as they have in India. But as already mentioned, their services and requirements are different after the like 26th November, 2008 incidents, where they without saving their own lives has sacrificed themselves without any hitch and without caring about their respective family members and wards. In other words, they are like our heroes and mentors who can guide us from the darkness of fear, militancy, corruption and other dark sides of life and so on. Now the question arises, if Gandhi would have been alive today, what would have been his reaction/opinion to the police and its functioning? Would he have some thing different in his mind now what he had been in his mind before the partition or would he be going to start some Satyagraha in the form of some improvement in the functioning of the police administration? Really these questions or rather night mares can come to any one’s mind, when there is too much confusion is prevailing in our minds, when there is too much corruption in the society and when the polices working is also in the questioning because of one or the other case throughout the India. It is matter of great concern that we have to thing over our administration and our practical approach because the police personals are also like us, they are part and parcel of our society and among one of us, so why we all are pin pointing towards them.
A STUDY ON TALENT MANAGEMENT AND ITS IMPACT ON EMPLOYEE RETENTION IN SELECTED...IAEME Publication
The goal of this study was to see how talent management affected employee retention in the selected IT organizations in Chennai. The fundamental issue was the difficulty to attract, hire, and retain talented personnel who perform well and the gap between supply and demand of talent acquisition and retaining them within the firms. The study's main goals were to determine the impact of talent management on employee retention in IT companies in Chennai, investigate talent management strategies that IT companies could use to improve talent acquisition, performance management, career planning and formulate retention strategies that the IT firms could use. The respondents were given a structured close-ended questionnaire with the 5 Point Likert Scale as part of the study's quantitative research design. The target population consisted of 289 IT professionals. The questionnaires were distributed and collected by the researcher directly. The Statistical Package for Social Sciences (SPSS) was used to collect and analyse the questionnaire responses. Hypotheses that were formulated for the various areas of the study were tested using a variety of statistical tests. The key findings of the study suggested that talent management had an impact on employee retention. The studies also found that there is a clear link between the implementation of talent management and retention measures. Management should provide enough training and development for employees, clarify job responsibilities, provide adequate remuneration packages, and recognise employees for exceptional performance.
ATTRITION IN THE IT INDUSTRY DURING COVID-19 PANDEMIC: LINKING EMOTIONAL INTE...IAEME Publication
Globally, Millions of dollars were spent by the organizations for employing skilled Information Technology (IT) professionals. It is costly to replace unskilled employees with IT professionals possessing technical skills and competencies that aid in interconnecting the business processes. The organization’s employment tactics were forced to alter by globalization along with technological innovations as they consistently diminish to remain lean, outsource to concentrate on core competencies along with restructuring/reallocate personnel to gather efficiency. As other jobs, organizations or professions have become reasonably more appropriate in a shifting employment landscape, the above alterations trigger both involuntary as well as voluntary turnover. The employee view on jobs is also afflicted by the COVID-19 pandemic along with the employee-driven labour market. So, having effective strategies is necessary to tackle the withdrawal rate of employees. By associating Emotional Intelligence (EI) along with Talent Management (TM) in the IT industry, the rise in attrition rate was analyzed in this study. Only 303 respondents were collected out of 350 participants to whom questionnaires were distributed. From the employees of IT organizations located in Bangalore (India), the data were congregated. A simple random sampling methodology was employed to congregate data as of the respondents. Generating the hypothesis along with testing is eventuated. The effect of EI and TM along with regression analysis between TM and EI was analyzed. The outcomes indicated that employee and Organizational Performance (OP) were elevated by effective EI along with TM.
INFLUENCE OF TALENT MANAGEMENT PRACTICES ON ORGANIZATIONAL PERFORMANCE A STUD...IAEME Publication
By implementing talent management strategy, organizations would have the option to retain their skilled professionals while additionally working on their overall performance. It is the course of appropriately utilizing the ideal individuals, setting them up for future top positions, exploring and dealing with their performance, and holding them back from leaving the organization. It is employee performance that determines the success of every organization. The firm quickly obtains an upper hand over its rivals in the event that its employees having particular skills that cannot be duplicated by the competitors. Thus, firms are centred on creating successful talent management practices and processes to deal with the unique human resources. Firms are additionally endeavouring to keep their top/key staff since on the off chance that they leave; the whole store of information leaves the firm's hands. The study's objective was to determine the impact of talent management on organizational performance among the selected IT organizations in Chennai. The study recommends that talent management limitedly affects performance. On the off chance that this talent is appropriately management and implemented properly, organizations might benefit as much as possible from their maintained assets to support development and productivity, both monetarily and non-monetarily.
A STUDY OF VARIOUS TYPES OF LOANS OF SELECTED PUBLIC AND PRIVATE SECTOR BANKS...IAEME Publication
Banking regulations act of India, 1949 defines banking as “acceptance of deposits for the purpose of lending or investment from the public, repayment on demand or otherwise and withdrawable through cheques, drafts order or otherwise”, the major participants of the Indian financial system are commercial banks, the financial institution encompassing term lending institutions. Investments institutions, specialized financial institution and the state level development banks, non banking financial companies (NBFC) and other market intermediaries such has the stock brokers and money lenders are among the oldest of the certain variants of NBFC and the oldest market participants. The asset quality of banks is one of the most important indicators of their financial health. The Indian banking sector has been facing severe problems of increasing Non- Performing Assets (NPAs). The NPAs growth directly and indirectly affects the quality of assets and profitability of banks. It also shows the efficiency of banks credit risk management and the recovery effectiveness. NPA do not generate any income, whereas, the bank is required to make provisions for such as assets that why is a double edge weapon. This paper outlines the concept of quality of bank loans of different types like Housing, Agriculture and MSME loans in state Haryana of selected public and private sector banks. This study is highlighting problems associated with the role of commercial bank in financing Small and Medium Scale Enterprises (SME). The overall objective of the research was to assess the effect of the financing provisions existing for the setting up and operations of MSMEs in the country and to generate recommendations for more robust financing mechanisms for successful operation of the MSMEs, in turn understanding the impact of MSME loans on financial institutions due to NPA. There are many research conducted on the topic of Non- Performing Assets (NPA) Management, concerning particular bank, comparative study of public and private banks etc. In this paper the researcher is considering the aggregate data of selected public sector and private sector banks and attempts to compare the NPA of Housing, Agriculture and MSME loans in state Haryana of public and private sector banks. The tools used in the study are average and Anova test and variance. The findings reveal that NPA is common problem for both public and private sector banks and is associated with all types of loans either that is housing loans, agriculture loans and loans to SMES. NPAs of both public and private sector banks show the increasing trend. In 2010-11 GNPA of public and private sector were at same level it was 2% but after 2010-11 it increased in many fold and at present there is GNPA in some more than 15%. It shows the dark area of Indian banking sector.
EXPERIMENTAL STUDY OF MECHANICAL AND TRIBOLOGICAL RELATION OF NYLON/BaSO4 POL...IAEME Publication
An experiment conducted in this study found that BaSO4 changed Nylon 6's mechanical properties. By changing the weight ratios, BaSO4 was used to make Nylon 6. This Researcher looked into how hard Nylon-6/BaSO4 composites are and how well they wear. Experiments were done based on Taguchi design L9. Nylon-6/BaSO4 composites can be tested for their hardness number using a Rockwell hardness testing apparatus. On Nylon/BaSO4, the wear behavior was measured by a wear monitor, pinon-disc friction by varying reinforcement, sliding speed, and sliding distance, and the microstructure of the crack surfaces was observed by SEM. This study provides significant contributions to ultimate strength by increasing BaSO4 content up to 16% in the composites, and sliding speed contributes 72.45% to the wear rate
ROLE OF SOCIAL ENTREPRENEURSHIP IN RURAL DEVELOPMENT OF INDIA - PROBLEMS AND ...IAEME Publication
The majority of the population in India lives in villages. The village is the back bone of the country. Village or rural industries play an important role in the national economy, particularly in the rural development. Developing the rural economy is one of the key indicators towards a country’s success. Whether it be the need to look after the welfare of the farmers or invest in rural infrastructure, Governments have to ensure that rural development isn’t compromised. The economic development of our country largely depends on the progress of rural areas and the standard of living of rural masses. Village or rural industries play an important role in the national economy, particularly in the rural development. Rural entrepreneurship is based on stimulating local entrepreneurial talent and the subsequent growth of indigenous enterprises. It recognizes opportunity in the rural areas and accelerates a unique blend of resources either inside or outside of agriculture. Rural entrepreneurship brings an economic value to the rural sector by creating new methods of production, new markets, new products and generate employment opportunities thereby ensuring continuous rural development. Social Entrepreneurship has the direct and primary objective of serving the society along with the earning profits. So, social entrepreneurship is different from the economic entrepreneurship as its basic objective is not to earn profits but for providing innovative solutions to meet the society needs which are not taken care by majority of the entrepreneurs as they are in the business for profit making as a sole objective. So, the Social Entrepreneurs have the huge growth potential particularly in the developing countries like India where we have huge societal disparities in terms of the financial positions of the population. Still 22 percent of the Indian population is below the poverty line and also there is disparity among the rural & urban population in terms of families living under BPL. 25.7 percent of the rural population & 13.7 percent of the urban population is under BPL which clearly shows the disparity of the poor people in the rural and urban areas. The need to develop social entrepreneurship in agriculture is dictated by a large number of social problems. Such problems include low living standards, unemployment, and social tension. The reasons that led to the emergence of the practice of social entrepreneurship are the above factors. The research problem lays upon disclosing the importance of role of social entrepreneurship in rural development of India. The paper the tendencies of social entrepreneurship in India, to present successful examples of such business for providing recommendations how to improve situation in rural areas in terms of social entrepreneurship development. Indian government has made some steps towards development of social enterprises, social entrepreneurship, and social in- novation, but a lot remains to be improved.
OPTIMAL RECONFIGURATION OF POWER DISTRIBUTION RADIAL NETWORK USING HYBRID MET...IAEME Publication
Distribution system is a critical link between the electric power distributor and the consumers. Most of the distribution networks commonly used by the electric utility is the radial distribution network. However in this type of network, it has technical issues such as enormous power losses which affect the quality of the supply. Nowadays, the introduction of Distributed Generation (DG) units in the system help improve and support the voltage profile of the network as well as the performance of the system components through power loss mitigation. In this study network reconfiguration was done using two meta-heuristic algorithms Particle Swarm Optimization and Gravitational Search Algorithm (PSO-GSA) to enhance power quality and voltage profile in the system when simultaneously applied with the DG units. Backward/Forward Sweep Method was used in the load flow analysis and simulated using the MATLAB program. Five cases were considered in the Reconfiguration based on the contribution of DG units. The proposed method was tested using IEEE 33 bus system. Based on the results, there was a voltage profile improvement in the system from 0.9038 p.u. to 0.9594 p.u.. The integration of DG in the network also reduced power losses from 210.98 kW to 69.3963 kW. Simulated results are drawn to show the performance of each case.
APPLICATION OF FRUGAL APPROACH FOR PRODUCTIVITY IMPROVEMENT - A CASE STUDY OF...IAEME Publication
Manufacturing industries have witnessed an outburst in productivity. For productivity improvement manufacturing industries are taking various initiatives by using lean tools and techniques. However, in different manufacturing industries, frugal approach is applied in product design and services as a tool for improvement. Frugal approach contributed to prove less is more and seems indirectly contributing to improve productivity. Hence, there is need to understand status of frugal approach application in manufacturing industries. All manufacturing industries are trying hard and putting continuous efforts for competitive existence. For productivity improvements, manufacturing industries are coming up with different effective and efficient solutions in manufacturing processes and operations. To overcome current challenges, manufacturing industries have started using frugal approach in product design and services. For this study, methodology adopted with both primary and secondary sources of data. For primary source interview and observation technique is used and for secondary source review has done based on available literatures in website, printed magazines, manual etc. An attempt has made for understanding application of frugal approach with the study of manufacturing industry project. Manufacturing industry selected for this project study is Mahindra and Mahindra Ltd. This paper will help researcher to find the connections between the two concepts productivity improvement and frugal approach. This paper will help to understand significance of frugal approach for productivity improvement in manufacturing industry. This will also help to understand current scenario of frugal approach in manufacturing industry. In manufacturing industries various process are involved to deliver the final product. In the process of converting input in to output through manufacturing process productivity plays very critical role. Hence this study will help to evolve status of frugal approach in productivity improvement programme. The notion of frugal can be viewed as an approach towards productivity improvement in manufacturing industries.
A MULTIPLE – CHANNEL QUEUING MODELS ON FUZZY ENVIRONMENTIAEME Publication
In this paper, we investigated a queuing model of fuzzy environment-based a multiple channel queuing model (M/M/C) ( /FCFS) and study its performance under realistic conditions. It applies a nonagonal fuzzy number to analyse the relevant performance of a multiple channel queuing model (M/M/C) ( /FCFS). Based on the sub interval average ranking method for nonagonal fuzzy number, we convert fuzzy number to crisp one. Numerical results reveal that the efficiency of this method. Intuitively, the fuzzy environment adapts well to a multiple channel queuing models (M/M/C) ( /FCFS) are very well.
Harnessing WebAssembly for Real-time Stateless Streaming PipelinesChristina Lin
Traditionally, dealing with real-time data pipelines has involved significant overhead, even for straightforward tasks like data transformation or masking. However, in this talk, we’ll venture into the dynamic realm of WebAssembly (WASM) and discover how it can revolutionize the creation of stateless streaming pipelines within a Kafka (Redpanda) broker. These pipelines are adept at managing low-latency, high-data-volume scenarios.
Embedded machine learning-based road conditions and driving behavior monitoringIJECEIAES
Car accident rates have increased in recent years, resulting in losses in human lives, properties, and other financial costs. An embedded machine learning-based system is developed to address this critical issue. The system can monitor road conditions, detect driving patterns, and identify aggressive driving behaviors. The system is based on neural networks trained on a comprehensive dataset of driving events, driving styles, and road conditions. The system effectively detects potential risks and helps mitigate the frequency and impact of accidents. The primary goal is to ensure the safety of drivers and vehicles. Collecting data involved gathering information on three key road events: normal street and normal drive, speed bumps, circular yellow speed bumps, and three aggressive driving actions: sudden start, sudden stop, and sudden entry. The gathered data is processed and analyzed using a machine learning system designed for limited power and memory devices. The developed system resulted in 91.9% accuracy, 93.6% precision, and 92% recall. The achieved inference time on an Arduino Nano 33 BLE Sense with a 32-bit CPU running at 64 MHz is 34 ms and requires 2.6 kB peak RAM and 139.9 kB program flash memory, making it suitable for resource-constrained embedded systems.
A review on techniques and modelling methodologies used for checking electrom...nooriasukmaningtyas
The proper function of the integrated circuit (IC) in an inhibiting electromagnetic environment has always been a serious concern throughout the decades of revolution in the world of electronics, from disjunct devices to today’s integrated circuit technology, where billions of transistors are combined on a single chip. The automotive industry and smart vehicles in particular, are confronting design issues such as being prone to electromagnetic interference (EMI). Electronic control devices calculate incorrect outputs because of EMI and sensors give misleading values which can prove fatal in case of automotives. In this paper, the authors have non exhaustively tried to review research work concerned with the investigation of EMI in ICs and prediction of this EMI using various modelling methodologies and measurement setups.
A SYSTEMATIC RISK ASSESSMENT APPROACH FOR SECURING THE SMART IRRIGATION SYSTEMSIJNSA Journal
The smart irrigation system represents an innovative approach to optimize water usage in agricultural and landscaping practices. The integration of cutting-edge technologies, including sensors, actuators, and data analysis, empowers this system to provide accurate monitoring and control of irrigation processes by leveraging real-time environmental conditions. The main objective of a smart irrigation system is to optimize water efficiency, minimize expenses, and foster the adoption of sustainable water management methods. This paper conducts a systematic risk assessment by exploring the key components/assets and their functionalities in the smart irrigation system. The crucial role of sensors in gathering data on soil moisture, weather patterns, and plant well-being is emphasized in this system. These sensors enable intelligent decision-making in irrigation scheduling and water distribution, leading to enhanced water efficiency and sustainable water management practices. Actuators enable automated control of irrigation devices, ensuring precise and targeted water delivery to plants. Additionally, the paper addresses the potential threat and vulnerabilities associated with smart irrigation systems. It discusses limitations of the system, such as power constraints and computational capabilities, and calculates the potential security risks. The paper suggests possible risk treatment methods for effective secure system operation. In conclusion, the paper emphasizes the significant benefits of implementing smart irrigation systems, including improved water conservation, increased crop yield, and reduced environmental impact. Additionally, based on the security analysis conducted, the paper recommends the implementation of countermeasures and security approaches to address vulnerabilities and ensure the integrity and reliability of the system. By incorporating these measures, smart irrigation technology can revolutionize water management practices in agriculture, promoting sustainability, resource efficiency, and safeguarding against potential security threats.
Comparative analysis between traditional aquaponics and reconstructed aquapon...bijceesjournal
The aquaponic system of planting is a method that does not require soil usage. It is a method that only needs water, fish, lava rocks (a substitute for soil), and plants. Aquaponic systems are sustainable and environmentally friendly. Its use not only helps to plant in small spaces but also helps reduce artificial chemical use and minimizes excess water use, as aquaponics consumes 90% less water than soil-based gardening. The study applied a descriptive and experimental design to assess and compare conventional and reconstructed aquaponic methods for reproducing tomatoes. The researchers created an observation checklist to determine the significant factors of the study. The study aims to determine the significant difference between traditional aquaponics and reconstructed aquaponics systems propagating tomatoes in terms of height, weight, girth, and number of fruits. The reconstructed aquaponics system’s higher growth yield results in a much more nourished crop than the traditional aquaponics system. It is superior in its number of fruits, height, weight, and girth measurement. Moreover, the reconstructed aquaponics system is proven to eliminate all the hindrances present in the traditional aquaponics system, which are overcrowding of fish, algae growth, pest problems, contaminated water, and dead fish.
Using recycled concrete aggregates (RCA) for pavements is crucial to achieving sustainability. Implementing RCA for new pavement can minimize carbon footprint, conserve natural resources, reduce harmful emissions, and lower life cycle costs. Compared to natural aggregate (NA), RCA pavement has fewer comprehensive studies and sustainability assessments.
ACEP Magazine edition 4th launched on 05.06.2024Rahul
This document provides information about the third edition of the magazine "Sthapatya" published by the Association of Civil Engineers (Practicing) Aurangabad. It includes messages from current and past presidents of ACEP, memories and photos from past ACEP events, information on life time achievement awards given by ACEP, and a technical article on concrete maintenance, repairs and strengthening. The document highlights activities of ACEP and provides a technical educational article for members.
Advanced control scheme of doubly fed induction generator for wind turbine us...IJECEIAES
This paper describes a speed control device for generating electrical energy on an electricity network based on the doubly fed induction generator (DFIG) used for wind power conversion systems. At first, a double-fed induction generator model was constructed. A control law is formulated to govern the flow of energy between the stator of a DFIG and the energy network using three types of controllers: proportional integral (PI), sliding mode controller (SMC) and second order sliding mode controller (SOSMC). Their different results in terms of power reference tracking, reaction to unexpected speed fluctuations, sensitivity to perturbations, and resilience against machine parameter alterations are compared. MATLAB/Simulink was used to conduct the simulations for the preceding study. Multiple simulations have shown very satisfying results, and the investigations demonstrate the efficacy and power-enhancing capabilities of the suggested control system.
Understanding Inductive Bias in Machine LearningSUTEJAS
This presentation explores the concept of inductive bias in machine learning. It explains how algorithms come with built-in assumptions and preferences that guide the learning process. You'll learn about the different types of inductive bias and how they can impact the performance and generalizability of machine learning models.
The presentation also covers the positive and negative aspects of inductive bias, along with strategies for mitigating potential drawbacks. We'll explore examples of how bias manifests in algorithms like neural networks and decision trees.
By understanding inductive bias, you can gain valuable insights into how machine learning models work and make informed decisions when building and deploying them.
2. Gibran Dewanda, Widjiati and Sri Pantja Madyawati
http://www.iaeme.com/IJCIET/index.asp 431 editor@iaeme.com
1. INTRODUCTION
Developing countries are very related to industrial growth. Industrial growth has a positive
impact on improving people’s living standards. However, the negative impact that results in
environmental pollution which disrupts the environment ecosystem. One form of pollution is
water pollution due to organic substances, pathogenic substances, dangerous heavy metals, and
pesticides. One of the heavy metals that cause pollution is mercury. Mercury pollution in the
aquatic environment, both in the sea and rivers, will cause intoxication of marine organisms.
Mercury poisoning is usually caused by the habit of eating food from the sea originating from
waters contaminated by mercury (Palar, 2008). The whole body can easily absorb food
contaminated by methylmercury after being absorbed by methylmercury carried by the blood
to all body tissues (Paterson M and A.P. Talcott, 2006).
Mercury in contaminated fish or marine organisms, if consumed, will be digested and
absorbed by the digestive tract. The mercury absorbed will enter the bloodstream and be
distributed to certain organs, such as the liver, kidneys, and brain. Mercury is neurotoxic which
shows changes in cells in the cerebral cortex. The accumulation of mercury in the cerebrum
cortex will cause damage to pyramid cells (Despopoulus, 2000) exposure to large amounts of
mercury which occurs continuously. There will be the formation of free radicals in the body
which will cause a decrease in the body’s antioxidant activity and can cause disease. To
overcome this problem, extracellular antioxidants are usually needed in foods, such as vitamin
C, vitamin E, and curcumin (Bhagya, 2013).
Curcumin is a yellow pigment found in the plant rhizome of the genus curcuma (wahyuni
et al., 2018). Curcumin is expressed as a phytochemical that is safe to use, not toxic, or
teratogenic even when used at high doses. Curcumin can be used as an antioxidant compound.
The body needs antioxidants that can reduce free radical (purba and martosupono, 2009). In
addition, curcumin inhibits brain damage due to the activity of chemical mediators and at the
time of the occurrence of foreign objects into the brain (jayaprakasha, 2009). In vitro and in
vivo trials prove that curcumin is efficacious as an anti-amyloidogenic, antioxidant, and anti-
inflammatory drug that can prevent alzheimer’s disease (ringman et al., 2005). The purpose of
this study was to determine the effect of administering curcumin to mice (mus musculus)
exposed to methylmercury to decrease the number of pyramid cells that experienced necrosis.
2. MATERIALS & EXPERIMENTAL PROCEDURES
2.1. Materials and Equipment
This research was conducted in an experimental animal cage, Faculty of Veterinary,
Universitas Airlangga. The performance of histopathological preparations of mice brains was
carried out at the Veterinary Pathology Laboratory of the Faculty of Veterinary, Universitas
Airlangga.
The research material used in this study were as follows: (i) The experimental animals used
in this study were 20 male mice that were determined using the federe formula. Mice receiving
treatment were controlled by the criteria of 16 weeks of age with a body weight of 30 grams
divided into four groups. The mice were obtained from the Veterinaria Farma Center in
Surabaya. (ii) The equipment used in this study was a scale to measure the body weight of
mice, microscopes to examine histopathological preparations of cerebrum mice, mice cages,
sonde, syringes, surgical scissors, scalpels, tubes, and Petri disks. (iii) The research material
used was methylmercury in the form of Methylmercury (II) chloride with the chemical formula
(CH3ClHg) made by Sigma-Aldrich, distilled water, formalin, tube, and curcumin (Merck).
3. Potential of Curcumin in Pyramid Cell Necrosis of Mice (Mus Musculus) Due to Methylmercury
Exposure
http://www.iaeme.com/IJCIET/index.asp 432 editor@iaeme.com
The administration of curcumin was carried out in multilevel dosages of 150 mg/KgBW and
300 mg/KgBW.
2.2. Methods
The research design used was a laboratory experimental method. The study design used was a
completely randomized design (crd) with a factor of the number of pyramid cells that were
damaged with an assumption:
K- : Group of controlled mice given only with distilled water
K+ : Group of mice with 0.1 methylmercury at a dose of 0.0056 mg/kgbw for 30 days
P1 : Group of mice with 0.0056 mg/kgbw methylmercury per day and 150 mg/kgbw
curcumin for 30 days
P2 : Group of mice with 0.0056 mg/kgbw methylmercury and 300 mg/kgbw curcumin for
30 days
Histopathological assessment was carried out microscopically on histological changes that
occurred in the cerebral cortex of the brain of mice and focused on the number of pyramid cells
that experience cell necrosis (picnosis, karyorrhexis, and karyolysis). Observations were made
in five different fields of view. Observations were carried out in each of the five fields of view,
starting from the right, bottom, right, up and right side of the preparation then viewed under a
microscope with 400x magnification. The signs of cell damage observed are: (Price, S. A. And
Wilson, 2006)
I. Cells that undergo karyopyknotic will give a profile of the small nucleus, cells are
more basophilic, darker and obscure boundaries.
II. Cells that undergo karyorrhexis will give a profile of fragmented cell nucleus by
leaving the remnants of chromatin in the cell.
III. Cells that undergo karyolysis will give a profile of the cell nucleus that disappears.
Data collection in this study was carried out by: (i) Mice were adapted to the environment
around 7 days. During the adaptation process, mice were fed and given with water ad libitium.
(ii) Preparation of the treatment using 0.04 mg/KgBW dose (Johansson et al., 2007) (9) and it
was converted to the dose of mice 0.04 x 0.14 = 0.0056mg/KgBW. Toxic doses given were
based on fractions that did not kill mice but had the potential to cause toxic effects (Hamid et
al., 2014). The doses of curcumin were 150mg/kgBW and 300mg/kgBW (Aggarwal, 2007).
(iii) Experimental animals were divided randomly into four treatment groups with each
treatment consisting of 5 replications. (iv) Sampling was carried out on the 31st day. The brain
organs were taken and then histopathological preparations with Hematoxylin-Eosin Staining
was made. (v) Histopathological preparation was performed. (vi) Examination of brain
histopathology preparations was done using a microscope with 400x magnification on five
different fields of view.
Data in this study were analyzed using the Analysis of Variance (ANOVA) test with p
<0.05 if significant differences were found to be followed by Duncan’s multiple distance test.
3. RESULTS AND DISCUSSION
3.1. Result
The results showed that there were significant comparisons of the number of cerebrum pyramid
cells of mice (Mus musculus) which were damaged after being given with curcumin due to the
4. Gibran Dewanda, Widjiati and Sri Pantja Madyawati
http://www.iaeme.com/IJCIET/index.asp 433 editor@iaeme.com
exposure of methylmercury to which curcumin was administered and no curcumin was
administered.
Table 1. Analysis of the number of cerebrum pyramid cells of mice (Mus musculus) which have
necrosis due to exposure to methylmercury and given with curcumin.
Treatments
The mean number of pyramid cells necrosis ±
SD
K-
K+
P1
P2
2.56 a
± 0.83
23.28c
± 8.64
10.32b
± 4.93
5.80ab
± 2.97
Note: Different superscripts in the same column show significant differences (p<0.05)
K-: Negative control, only given with distilled water
K +: Positive control, exposed to methylmercury at a dose of 0.0056 mg/KgBW orally
P1: First treatment, exposed to methylmercury at a dose of 0.0056 mg/KgBW orally and
given with curcumin at a dose of 150 mg/KgBW orally
P2: Second treatment, exposed to methylmercury at a dose of 0.0056 mg/KgBW orally and
given with curcumin at a dose of 300mg/KgBW orally.
The results of the statistical analysis of variance (ANOVA) test showed a significant
difference of 0.000 (p<0.05). Furthermore, to find out the differences between treatments, a
further test was conducted with Duncan’s test with the results showing negative control
treatment which was only given aquadest (K-) significantly different (p<0.05) with P1 and K+.
Positive treatment which was only exposed to methylmercury (K+) was significantly different
from K-, P1, and P2 which were given with methylmercury and curcumin. The profile of
cerebrum pyramid cells in mice can be seen in Figure 1.
Figure 1. Pyramid cell cerebrum histopathology (Hematoxylin-Eosin staining; 1000 times
magnification. Blue arrow shows normal pyramid cells and red arrows show pyramid cells that
experience necrosis). Normal pyramid cells of the cell nucleus are clear, whereas necrotic pyramid
cells have a darker cell nucleus.
5. Potential of Curcumin in Pyramid Cell Necrosis of Mice (Mus Musculus) Due to Methylmercury
Exposure
http://www.iaeme.com/IJCIET/index.asp 434 editor@iaeme.com
This study also found a comparison between the number of decreases in pyramid cells that
experienced necrosis between the control group and the treatment group. The results of the
examination of pyramid cell histopathology cerebrum mice are shown in Figure 2 below:
Figure 2. Analysis of the number of mean cerebral pyramid cell necrosis of mice exposed to
methylmercury and given with curcumin.
The results of K+ treatment as a positive control showed the highest value with a large
number of pyramid cells experiencing necrosis. Treatments P1 and P2 showed a reduction in
the number of pyramid cells that experienced necrosis with the administration of curcumin.
The decrease in the number of cells experiencing necrosis was directly proportional to the
increase in the dose given. The decrease in each treatment was P1 of 150mg/kgBW curcumin
at 10.32 and P2 of 300mg/kgBW curcumin at 5.80.
3.2. Discussion
Methylmercury (Hg2+
) contained in the body induces apoptosis in neuron cells. In addition,
methylmercury is neurotoxic which shows changes in cells in the cerebral cortex.
Accumulation of mercury in the cerebral cortex will cause damage to pyramid cells
(Despopoulus, 2000).
The results of counting the number of necrotizing pyramid cells showed significant results
(p<0.05) among all treatments. In the positive control group which was only given with
methylmercury (K+) as much as 23.28 ± 8.642 showed an increase in the number of necrosis
cells when compared to negative control which was only given with distilled water (K-) as
much as 2.56 ± 0.832. This shows that methylmercury can increase pyramid cell necrosis due
to exposure to methylmercury. Longer exposure to methylmercury will reduce the number of
normal cells. This can be seen in the treatment of three weeks and six weeks, the number of
living cells decreases on exposure to mercury for six weeks. Cell necrosis was very large. In
this study, it was seen that most of the pyramid cells in the brain experienced necrosis due to
exposure to methylmercury (Bhagya, 2013).
Methylmercury exposure can produce very varied effects. All forms of the effects of
methylmercury depend on the magnitude and duration of exposure and also the age and health
status of the individual. Exposure to methylmercury at large levels can produce effects on the
nervous, respiratory, kidney, immune system, pregnancy, dermatology, and various other
6. Gibran Dewanda, Widjiati and Sri Pantja Madyawati
http://www.iaeme.com/IJCIET/index.asp 435 editor@iaeme.com
effects. The nervous system is the main target of metal toxicity, especially organic metal
compounds. Methylmercury, which has fat soluble properties, easily passes through the blood-
brain barrier and enters the nervous system (Fouad et al., 2009).
As one of the strongest thiol binding agents, Hg2+
specifically binds to thiols in cells. The
direct chemical interactions between Hg2+
and thiol groups of proteins or nonprotein molecules
may play an important role in Hg-induced neurotoxicity, resulting in disorders of endogenous
antioxidants and non-enzymatic antioxidants. NPSH acts as a nucleophilic binder of many of
its compounds and metabolites. Binding of NPSH metabolites through enzymatic and chemical
mechanisms and processes plays an important role in protecting against oxidative stress caused
by reactive oxygen species (ROS). About 90% NPSH is glutathione (GSH), which is the most
intracellular low molecular weight sulfhydryl compound (Ayer et al., 2010). GSH can join
Hg2+
directly and form a series that prevents Hg2+
from binding to cellular proteins. In addition,
GSH can react with free radicals, eliminating excessive ROS produced by oxidants.
Free radicals contained in mercury can cause lipid peroxidation, protein oxidation, ROS,
and ultimately cause brain neuron cell death. This is not surprising because the brain is more
susceptible to oxidative damage than other organs or tissues because of the high levels of
oxygen consumption, high polyunsaturated fat content, and the relative lack of antioxidant
enzymes (Ayer et al., 2010). In the normal state, the antioxidant defense system can easily
overcome the free radicals formed. The time of increased use of oxygen free radical production
is believed to be very instrumental in causing cardiovascular disease, cancer, Alzheimer’s
disease and Parkinson’s (Musalmah, 2009).
Histopathological description of the group given with Curcumin (P1 and P2) showed a
difference with the positive control group which was only exposed to methylmercury. The
number of pyramid cell necrosis was significantly different compared to the group exposed to
only 0.0056 mg/kgBW (K+). The P1 group, which was given a dose of 150 mg/kg of curcumin,
showed a decrease in the amount of necrosis compared to the K+ group which was only
exposed to methylmercury. P2 group, which administered by the dose of 300 mg/kgBW
curcumin, showed a decrease in the number of pyramid cells. The positive control group (K+)
exposed only to methylmercury showed results of 23.28 ± 8.642, while the treatment given
with curcumin (P1) was 10.32 ± 4.937 and (P2) was 5.80 ± 2.9777. This indicates that curcumin
can reduce the number of pyramid cell necrosis.
Curcumin has antioxidant activity. Antioxidants based on how they work function as an
antidote to the effects of free radicals. Besides, antioxidants can also prevent the occurrence of
free radical ion bonds with cells, repair cells and tissues that have been damaged so that no
damage to cells (Capelli and Cysewski, 2010). Curcumin has the function of repairing damaged
cells and tissues caused by free radicals. Curcumin has the properties of Scavenger and
Chelators. The nature of Scavenger is to function to bind oxygen so as to prevent the occurrence
of oxidation reactions. Whereas, Chelators properties function to bind metals and are able to
catalyze the occurrence of oxidation reactions.
Curcumin has gone through clinical trials of its effectiveness as a therapy in phases I, II
and III, so that even at high doses (12g/day for three months) and it is still safe to use. The
study shows that this is related to the ability of curcumin as an antioxidant, anti-inflammatory,
anti-cholestatic, anti-fibrogenesis, and anti-carcinogenic (Oktaviana, 2010). The human body
naturally has an antioxidant system defense. Antioxidants in curcumin work by protecting
lipids from the process of peroxidation by free radicals. When free radicals get electrons from
antioxidants, the free radicals no longer need to attack cells and the oxidation chain reaction
will be broken. After giving electrons, antioxidants become free radicals. By definition,
7. Potential of Curcumin in Pyramid Cell Necrosis of Mice (Mus Musculus) Due to Methylmercury
Exposure
http://www.iaeme.com/IJCIET/index.asp 436 editor@iaeme.com
antioxidants in this state are dangerous because they have the ability to change electrons
without becoming reactive.
Curcumin is known to protect biomembrane from peroxidative damage. Lipid peroxidation
is known as a chain reaction mediated by free radicals, which causes damage to cell
membranes. The inhibition of peroxidation by curcumin is caused by the rinsing of reactive
free radicals involved in peroxidation. Most antioxidants have phenolic group or diketone
group functions. Curcumin is a unique antioxidant, which contains a variety of functional
groups, including the B-diketo group, double carbon-carbon bonds, and phenyl rings
containing varying amounts of hydroxyl and methoxy substituents (García-Niño and Pedraza-
Chaverrí, 2014).
Curcumin as an antioxidant performs extraordinary H-atom donors from the central
methylene group rather than from a phenolic group dissolved in acid and neutral acetonitrile
(S, 2002). Besides that, curcumin is a classic phenolic chain-breaking antioxidant, donating H-
atoms from phenolic groups (Jovanovic et al., 1999). This phenolic group is very important for
free radical scavenging activities by increasing the activity of the methoxy group (Barclay et
al., 2000).
The most common mechanism by which free radicals can fight antioxidant defenses is by
attacking the biochemical components in the body and forming hydroperoxide. In this
pathophysiological form, cells will start producing free radicals in large quantities, due to
exogenous stress (chemical, physical and biological elements) and or nine metabolic activities
(especially in the plasma membrane, mitochondria, endoplasmic reticulum, and cytosol).
Cytosols, including dangerous hydroxyl (HOH) radicals, are one of the most dangerous
reactive oxygen species (ROS). Hydroxyl radicals can attack every type of molecule (including
carbohydrates, fats, amino acids, peptides, proteins, nucleotides, nucleic acids, and others). As
a result of this process, each molecule will lose one electron and then become radical. After
that, a radical chain reaction will occur due to the presence of oxygen molecules (through
breathing), and the formation of hydroperoxide (ROOH), a type of Reactive Oxygen
Metabolites (ROMs). Although hydroperoxide is a relatively stable type of chemical, it has the
potential to form free radicals again and can oxidize other target molecules. Furthermore, the
cell will pull out hydroperoxide in the extracellular environment, including blood,
cerebrospinal fluid, pleural fluid and others (Priyadarsini et al., 2003).
4. CONCLUSION
Curcumin can reduce the number of pyramid cells that are damaged due to exposure to
methylmercury. The decrease in the number of necrotic pyramid cells is directly proportional
to the increase in the dose given. The highest decrease in the number of pyramid cell necrosis
is in the provision of curcumin at a dose of 300 mg/KgBW.
ACKNOWLEDGEMENTS
The authors would like to thank every lecture in Faculty of Veterinary, Universitas Airlangga,
Surabaya, Indonesia for the support to finish this paper
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