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Plant Bioreactor
By
Avinash tiwari
M.Tech. biotechnology
2nd year (3rd sem)
201710902010002
INTRODUCTION
 Device or vessel, which are designed to provide an effective
environment for conversion of one material into some product by
appropriate biochemical reactions
 like-enzymes, microorganisms, cells of animals and plants,
subcellular structures such as chloroplasts and mitochondria
For- cell growth, enzyme production, biocatalysis, food
production, milk processing, tissue engineering, algae production,
protein synthesis, & anaerobic digestion
CLASSIFICATION
Bioreactor
Animal Plant Microbes
On the Basis of Operational Conditions and
The Nature of the Process
STRATEGIES FOR PLANT
Plant
1. Plant cell
bioreactor
2.Plant as bioreactor
On the basis of dynamics
ON THE BASIS OF CELLS TO CLUTURE
Seed-based plant bioreactors
Plant Suspension Cultures
Hairy Root System Bioreactor
Chloroplast bioreactor
VACCINE ANTIGENS
Antigens like-
• Insulin
• Rotavirus enterotoxin
• Anthrax lethal factor
• HIV antigen
• Foot and mouth disease virus antigen
• Heat stable toxin
THERAPEUTIC PRODUCTS
• The first successful production of a functional antibody, namely a
mouse immunoglobulin IgGI in plants, was reported in 1989.
• In 1992, C.J. Amtzen and co-workers- expressed hepatitis B surface
antigen in tobacco to produce immunologically active ingredients
via genetic engineering of plants
Nutritional components
• β-carotene (Naqvi et al., 2009),
• Lycopene (Fraser et al., 2002),
• Flavonoid (Butelli et al., 2008),
• Nutraceuticals (Kang et al., 2009),
• Fatty acid (Hoffmann et al., 2008),
• Vitamines (Nunes et al., 2009),
• Minerals (Lee & An, 2009) &
• Carbohydrates (Regina et al., 2006).
Biodegradable plastics
• Polyhydroxyalkanoates: biodegradable polymers which occur
naturally in plants.
• Plant was engineered to produce-
 PHAs or PHBs in the various plant cell compartments
Industrial products
• Hgc- Most expensive Drug
• hST (Human somatotropin)
• rHLF (Recombinant human lactoferrin)
• Synthetic fiber: Produced from Potato and tobacco.
ADVANTAGE & DISADVANTAGE
• Low cost source.
• Simple & Cost effective.
• Plant pathogens do not infect humans or animals.
• Produce large biomass.
• Easy storage for long time.
Plant proteins have different sugar residues from human or
animal proteins.
Plant bioreactor

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Plant bioreactor

  • 1. Plant Bioreactor By Avinash tiwari M.Tech. biotechnology 2nd year (3rd sem) 201710902010002
  • 2. INTRODUCTION  Device or vessel, which are designed to provide an effective environment for conversion of one material into some product by appropriate biochemical reactions  like-enzymes, microorganisms, cells of animals and plants, subcellular structures such as chloroplasts and mitochondria For- cell growth, enzyme production, biocatalysis, food production, milk processing, tissue engineering, algae production, protein synthesis, & anaerobic digestion
  • 3. CLASSIFICATION Bioreactor Animal Plant Microbes On the Basis of Operational Conditions and The Nature of the Process
  • 4. STRATEGIES FOR PLANT Plant 1. Plant cell bioreactor 2.Plant as bioreactor
  • 5. On the basis of dynamics
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  • 8. ON THE BASIS OF CELLS TO CLUTURE Seed-based plant bioreactors Plant Suspension Cultures Hairy Root System Bioreactor Chloroplast bioreactor
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  • 10. VACCINE ANTIGENS Antigens like- • Insulin • Rotavirus enterotoxin • Anthrax lethal factor • HIV antigen • Foot and mouth disease virus antigen • Heat stable toxin
  • 11. THERAPEUTIC PRODUCTS • The first successful production of a functional antibody, namely a mouse immunoglobulin IgGI in plants, was reported in 1989. • In 1992, C.J. Amtzen and co-workers- expressed hepatitis B surface antigen in tobacco to produce immunologically active ingredients via genetic engineering of plants
  • 12. Nutritional components • β-carotene (Naqvi et al., 2009), • Lycopene (Fraser et al., 2002), • Flavonoid (Butelli et al., 2008), • Nutraceuticals (Kang et al., 2009), • Fatty acid (Hoffmann et al., 2008), • Vitamines (Nunes et al., 2009), • Minerals (Lee & An, 2009) & • Carbohydrates (Regina et al., 2006).
  • 13. Biodegradable plastics • Polyhydroxyalkanoates: biodegradable polymers which occur naturally in plants. • Plant was engineered to produce-  PHAs or PHBs in the various plant cell compartments
  • 14. Industrial products • Hgc- Most expensive Drug • hST (Human somatotropin) • rHLF (Recombinant human lactoferrin) • Synthetic fiber: Produced from Potato and tobacco.
  • 15. ADVANTAGE & DISADVANTAGE • Low cost source. • Simple & Cost effective. • Plant pathogens do not infect humans or animals. • Produce large biomass. • Easy storage for long time. Plant proteins have different sugar residues from human or animal proteins.