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ENT 502 Insect Anatomy, Physiology and Nutrition
Course Teachers : Dr. S. Raguraman Ph.D (Associate
professor)
Present By
Logeswaran V
2020505014
I M.Sc Entomology
Physiology of Integuments and
Moulting in Insects
• Physiology is concerned with functions of organism
and its parts. Greek word-
‘physio’ means ‘physical (working/function)’
‘logy’ means study.
Integument:
Body wall or exoskeleton.
Ectodermal in origin.
• Epidermis (or
hypodermis)
• Basement
membrane
• Cuticle: Formed by the secretory products of the
epidermis.
• The cuticle consists of the outer non-chitinous
Epicuticle and the inner chitinous Procuticle.
Epicuticle:
Four sub layers, viz.
(i) Tectocuticle or cement layer
(ii) wax layer
(iii) Polyphenol layer
(iv) Cuticulin layer.
• Procuticle:
The procuticle is differentiated in to Exocuticle and
Endocuticle.
i. Endocuticle: Innermost and
thickest layer. Made up of
chitin and arthropodin. This
layer is colourless, soft and
flexible.
ii. Exocuticle: Outer layer, much
thicker with the composition
of chitin and sclerotin. This
layer is dark in colour and
rigid.
• Cuticle is composed of the following constituents:
 Chitin
 Proteins
 Lipids
 Polyphenols
 Cuticular enzymes
 Cuticular pigments
 Water
 Inorganic salts - Lime is deposited in cuticle in a few
aquatic insects.
• Nitrogenous polysaccharide named by Odier (1823).
• It is water insoluble but soluble in dilute acids, alkalies and organic
solvents.
• Made of long chains of acetylated glucosamine residues
N-Acetyl glucosamine (C8H13O5N)n
• Adjacent chains are held together by β –glycosidic linkages.
1-4 -linkage
N-acetyl-D-
glucosamine
glucosamine
Ranges from 25-55%
Proteins are of 3 types:
 Arthropodin : Water-soluble protein found in the endocuticle.
Untanned, elastic protein.
 Resilin: Rubber-like protein occurs as colourless cuticular
ligament such as wing-hinge ligament, clpeo-labral suture, etc.
Elastic in nature.
 Sclerotin: Tanned protein. Occurs in the exocuticle. It is in fact
tanned athropodin. Water insoluble.
Ranges from 25- 37%
 These are mostly contained in the wax layers of the epicuticle,
which average about 30 monolayers of wax molecules.
• Polyphenols and their quinone derivates play a role in
hardening and darkening (sclerotization) of the cuticle.
• The best known cuticular enzyme is “Tyrosinase enzyme system”
responsible for oxidation of tyrosine.
• For digestion of old cuticle the moulting fluid contains chitinase
(for digestion of protein and carbohydrate part of cuticle).
•These are metabolic products of substances ingested
with food.
e.g: Melanins, Carotenoids and Pterines
• A single layer of epidermal cells which are differentiated in to
(i) Ordinary epidermal cells
(ii) Specialized epidermal cells forming the dermal glands
(iii) Large occasional cells as well as the oenocytes
• It is a thin inner limiting
membrane on the under
surface of the epidermis
formed by its secretion or
by certain dead blood
cells.
 Composed of Chitin –Strong, rigid, and flexible.
 Provides large area for muscle attachment.
 Conserves water, minimizes water loss and hence
prevents desiccation.
 Prevents mechanical injury.
 Protective Coloration.
 Act as a tool for various activities like digging,
oviposition, and preying.
 Gives physical strength without making the insect
body heavier.
• Periodical process of shedding the old cuticle
accompanied by the formation of new cuticle
is known as moulting or ecdysis.
• Exuvia: The cuticular parts discarded during
moulting is known as exuvia.
• Stadium: The time interval between the two
subsequent moulting is called as stadium.
• Instar: The form assumed by the insect in any
stadium is called as instar.
20-Hydroxy ecdysone
Formula: C27H44O7
Endocrine gland in prothoracic region release
Ecdyson (20-Hydroxy Ecdyson)
Behavioral changes
 Larvae stops feeding & become inactive.
Changes in Epidermis
Cell size increase, Activities enhanced. Proteins &
Enzyme contents increase and thus tention cause loosening
the cuticle
Detachment of cuticle from epidermis is occurred.
1
2
3 Apolysis
Formation of sub cuticular space
In this stage the space is formed in sub cuticular area.
Secretion of Gel
Secretion of moulting gel in to sub cuticular space that
is rich in chitinase and protease.
New epicuticle layer formation
Cuticulin layers is laid on epidermis.
Pro cuticle layer formation
Pro cuticle layer is formed below epicuticle.
4
5
6
7
Activation of molting gel
The gel is converting into molting fluid and digest and
absorbed the endo cuticle.
Wax layer formation
In this stage the wax layer is formed.
Cement layer formation
Dermal gland produce cement layer.
8
9
10
Rupturing of old cuticle
As the water and air is absorbed and thus pressure is
generated by blood to head and thorax and “ecdysial line”
rupturing take place.
Moulting involved in 2 steps
Moulting
Removal of old cuticle
Due to peristaltic movement of the body and
Lubricant action of molting fluid helps in removal.
11
1
2
Formation of exo cuticle
The outer layer of pro cuticle develops as
exocuticle through adittion of protein and
tanning by Bursicon hormone.
Formation of endo cuticle
The below layer of pro cuticle develops as
endocuticle. This layer increase in thickness.
12
13
Control of moulting
 Corpora allata secretes Juvenile hormone (JH) or
Neotinin, there by inhibit metamorphosis. This CA
hormone(s) is therefore sometimes also called as
“Inhibitory or Status Quo” Hormone.
Corpora allata glands were discovered by JANET
during 1899.
JH first extracted from the abdomen of Hyalophora
cercopia (Moth).
JUVENILE HORMONE/NEOTININ
Neurosecretory hormone that controls Tanning or
Sclerotization and mechanical properties of the cuticle
during and after a moult.
Found in most ganglia of the CNS.
Bursicon:
Ecdysone / Moulting hormone/ Ecdysteriod
Neurosecretory cells activate prothoracic glands to secrete
ECDYSONE.
First experimental proof about the importance of
Prothoracic glands was provided by a Japanese worker,
Fukuda 1940 while working with silkworm.
Moulting of Cicada
• Chapman,R.F. 1982. The Insects – Structure and function.
Macmillian India Ltd.,Bangalore. 501-528.
• Duntson PA. 2004. The Insects: Structure, Function and
Biodiversity. Kalyani Publ., New Delhi.
• Wigglesworth VB.1984. Insect Physiology. 8th Ed. Chapman &
Hall, New York.
• Ragumoorthi, K.N. & Venkatasamy, Balasubramani & M R,
Srinivasan & Natarajan, N. (2011). Insecta- an introduction. 2nd
Edition. AE Publication, Coimbatore. 36-41.
References
Physiology of Integuments and moulting in insects

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Physiology of Integuments and moulting in insects

  • 1. ENT 502 Insect Anatomy, Physiology and Nutrition Course Teachers : Dr. S. Raguraman Ph.D (Associate professor) Present By Logeswaran V 2020505014 I M.Sc Entomology Physiology of Integuments and Moulting in Insects
  • 2. • Physiology is concerned with functions of organism and its parts. Greek word- ‘physio’ means ‘physical (working/function)’ ‘logy’ means study. Integument: Body wall or exoskeleton. Ectodermal in origin.
  • 3. • Epidermis (or hypodermis) • Basement membrane • Cuticle: Formed by the secretory products of the epidermis.
  • 4. • The cuticle consists of the outer non-chitinous Epicuticle and the inner chitinous Procuticle. Epicuticle: Four sub layers, viz. (i) Tectocuticle or cement layer (ii) wax layer (iii) Polyphenol layer (iv) Cuticulin layer.
  • 5. • Procuticle: The procuticle is differentiated in to Exocuticle and Endocuticle. i. Endocuticle: Innermost and thickest layer. Made up of chitin and arthropodin. This layer is colourless, soft and flexible. ii. Exocuticle: Outer layer, much thicker with the composition of chitin and sclerotin. This layer is dark in colour and rigid.
  • 6. • Cuticle is composed of the following constituents:  Chitin  Proteins  Lipids  Polyphenols  Cuticular enzymes  Cuticular pigments  Water  Inorganic salts - Lime is deposited in cuticle in a few aquatic insects.
  • 7. • Nitrogenous polysaccharide named by Odier (1823). • It is water insoluble but soluble in dilute acids, alkalies and organic solvents. • Made of long chains of acetylated glucosamine residues N-Acetyl glucosamine (C8H13O5N)n • Adjacent chains are held together by β –glycosidic linkages. 1-4 -linkage N-acetyl-D- glucosamine glucosamine Ranges from 25-55%
  • 8. Proteins are of 3 types:  Arthropodin : Water-soluble protein found in the endocuticle. Untanned, elastic protein.  Resilin: Rubber-like protein occurs as colourless cuticular ligament such as wing-hinge ligament, clpeo-labral suture, etc. Elastic in nature.  Sclerotin: Tanned protein. Occurs in the exocuticle. It is in fact tanned athropodin. Water insoluble. Ranges from 25- 37%  These are mostly contained in the wax layers of the epicuticle, which average about 30 monolayers of wax molecules.
  • 9. • Polyphenols and their quinone derivates play a role in hardening and darkening (sclerotization) of the cuticle. • The best known cuticular enzyme is “Tyrosinase enzyme system” responsible for oxidation of tyrosine. • For digestion of old cuticle the moulting fluid contains chitinase (for digestion of protein and carbohydrate part of cuticle). •These are metabolic products of substances ingested with food. e.g: Melanins, Carotenoids and Pterines
  • 10. • A single layer of epidermal cells which are differentiated in to (i) Ordinary epidermal cells (ii) Specialized epidermal cells forming the dermal glands (iii) Large occasional cells as well as the oenocytes • It is a thin inner limiting membrane on the under surface of the epidermis formed by its secretion or by certain dead blood cells.
  • 11.  Composed of Chitin –Strong, rigid, and flexible.  Provides large area for muscle attachment.  Conserves water, minimizes water loss and hence prevents desiccation.  Prevents mechanical injury.  Protective Coloration.  Act as a tool for various activities like digging, oviposition, and preying.  Gives physical strength without making the insect body heavier.
  • 12.
  • 13. • Periodical process of shedding the old cuticle accompanied by the formation of new cuticle is known as moulting or ecdysis. • Exuvia: The cuticular parts discarded during moulting is known as exuvia. • Stadium: The time interval between the two subsequent moulting is called as stadium. • Instar: The form assumed by the insect in any stadium is called as instar.
  • 14. 20-Hydroxy ecdysone Formula: C27H44O7 Endocrine gland in prothoracic region release Ecdyson (20-Hydroxy Ecdyson)
  • 15. Behavioral changes  Larvae stops feeding & become inactive. Changes in Epidermis Cell size increase, Activities enhanced. Proteins & Enzyme contents increase and thus tention cause loosening the cuticle Detachment of cuticle from epidermis is occurred. 1 2 3 Apolysis
  • 16. Formation of sub cuticular space In this stage the space is formed in sub cuticular area. Secretion of Gel Secretion of moulting gel in to sub cuticular space that is rich in chitinase and protease. New epicuticle layer formation Cuticulin layers is laid on epidermis. Pro cuticle layer formation Pro cuticle layer is formed below epicuticle. 4 5 6 7
  • 17.
  • 18. Activation of molting gel The gel is converting into molting fluid and digest and absorbed the endo cuticle. Wax layer formation In this stage the wax layer is formed. Cement layer formation Dermal gland produce cement layer. 8 9 10
  • 19. Rupturing of old cuticle As the water and air is absorbed and thus pressure is generated by blood to head and thorax and “ecdysial line” rupturing take place. Moulting involved in 2 steps Moulting Removal of old cuticle Due to peristaltic movement of the body and Lubricant action of molting fluid helps in removal. 11 1 2
  • 20. Formation of exo cuticle The outer layer of pro cuticle develops as exocuticle through adittion of protein and tanning by Bursicon hormone. Formation of endo cuticle The below layer of pro cuticle develops as endocuticle. This layer increase in thickness. 12 13
  • 21.
  • 23.  Corpora allata secretes Juvenile hormone (JH) or Neotinin, there by inhibit metamorphosis. This CA hormone(s) is therefore sometimes also called as “Inhibitory or Status Quo” Hormone. Corpora allata glands were discovered by JANET during 1899. JH first extracted from the abdomen of Hyalophora cercopia (Moth). JUVENILE HORMONE/NEOTININ
  • 24. Neurosecretory hormone that controls Tanning or Sclerotization and mechanical properties of the cuticle during and after a moult. Found in most ganglia of the CNS. Bursicon: Ecdysone / Moulting hormone/ Ecdysteriod Neurosecretory cells activate prothoracic glands to secrete ECDYSONE. First experimental proof about the importance of Prothoracic glands was provided by a Japanese worker, Fukuda 1940 while working with silkworm.
  • 26. • Chapman,R.F. 1982. The Insects – Structure and function. Macmillian India Ltd.,Bangalore. 501-528. • Duntson PA. 2004. The Insects: Structure, Function and Biodiversity. Kalyani Publ., New Delhi. • Wigglesworth VB.1984. Insect Physiology. 8th Ed. Chapman & Hall, New York. • Ragumoorthi, K.N. & Venkatasamy, Balasubramani & M R, Srinivasan & Natarajan, N. (2011). Insecta- an introduction. 2nd Edition. AE Publication, Coimbatore. 36-41. References