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IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
e-ISSN: 2278-3008, p-ISSN:2319-7676. Volume 10, Issue 6 Ver. II (Nov - Dec. 2015), PP 11-14
www.iosrjournals.org
DOI: 10.9790/3008-10621114 www.iosrjournals.org 11 | Page
Physico-chemical Characteristics of Water Quality for Culturing
the Freshwater Mussel Lamellidens marginalis in Pond and
Laboratory
Nirmala Natarajan1
and R. Susithira2
1
Associate Professor in Zoology, Periyar E.V.R. College (Autonomous), Tiruchirappalli - 620 023.
2
Teacher Educator, Urban Resource Center, SSA, Woraiyur, Tiruchirappalli - 620 003.
Abstract: Physicochemical characteristics viz., turbidity, pH, temperature, dissolved oxygen, salinity, hardness
and alkalinity of water were studied in the pond and laboratory for culturing the Freshwater Mussel
Lamellidens marginalis. The optimal ranges for the survival of Mussels were detected for the culture
maintenance. The data on the ecological factors of the pond and laboratory water have been studied for a
period of one year (from July 2013 to June 2014) and discussed with the light of similar work. The findings of
the present study confirmed that the physico-chemical parameters appeared to be varied due to the nature of
water as well as season.
Keywords: Freshwater mussel, Lamellidens marginalis, optimal ranges, physicochemical parameters.
I. Introduction
Water is a soul of nature which covers seven tenth of the earth’s surface. Water is an elixir of life, a
precious gift of nature to mankind and millions of other species living on the earth. Water resources comprising
of surface water viz., rivers, lakes and ponds, ground water, marine and coastal waters, support all living beings
including human beings. India has vast fresh water resources in the form of both lentic and lotic ecosystems.
The perennial reservoirs play an important role for domestic, agriculture and aquaculture as a valuable water
resource. The lentic ecosystems have long attracted attention of ecologists, both for their importance as a source
of drinking water and the development of fisheries [1].
Therefore, the attention is given on the physico-chemical factors which affect the aquatic inhabitants
[2]. Wetlands are very productive ecosystems, which help in the regulation of biological cycles, maintenance of
water quality, nutrient movement and support for food chains. In addition, they provide refuge for endangered
species of plants and animals and economic benefits. The natural aquatic resources are causing heavy and varied
pollution in aquatic environment leading to pollute water quality and depletion of aquatic biota [3]. Pollution
refers to undesirable changes in the physical, chemical or biological characteristics of our environment. This has
adversely affected the humans and other species of our biosphere directly or indirectly [4].
The Freshwater Molluscs constitute an important part of the ecosystem. Their participation in the way
of life in many organisms has made them significant partners in the ecological communities [5]. Freshwater
Mussels are widely distributed throughout the world occurring in both lotic and lentic systems [6]; [7]. In India ,
freshwater bodies show grate seasonal variations in the physicochemical factors which affect the natural
populations of Freshwater Mussels and consequently, transmission of disease. In view of the above, the present
study deals with the assessment of physic-chemical characteristics of a freshwater pond as well as the water
present in the laboratory for the cultural and survival of the experimental animal Freshwater Mussel, L.
marginalis.
II. Materials and Methods
The study was carried out in July 2013 to June 2014 in K. Sathanur, Moorthy Nagar pond located in
Tiruchirappalli of Tamil Nadu and in the laboratory tap water. Water samples were collected for physico-
chemical analyses in sterile bottles and taken to the laboratory aseptically. The collection was usually completed
during morning hours between 6:00 a.m. to 10:00 a.m. For each sampling event, odour (Odo), turbidity (Tur),
temperature (Tem), pH and dissolved oxygen (DO) were monitored at the sampling sites while total dissolved
solids (TDS), salinity (Sali.), total hardness (TH), calcium (Ca), magnesium (Mg), sodium (Na) and
potassium (K) were analyzed in the laboratory in accordance with the methods of FAO (1997) and APHA
(1998).
Physico-chemical Characteristics of Water Quality for Culturing the Freshwater Mussel…
DOI: 10.9790/3008-10621114 www.iosrjournals.org 12 | Page
III. Results and Discussion
The Physico-chemical data of the pond water situated at K. sathanur and laboratory tap water for 13
physico-chemical parameters are presented in Table-1 and Table-2 respectively. The obtained results were
compared with Standard prescribed under APHA [8]. The two water samples of pond and laboratory are
colourless and also with no objectionable odour. The turbidity of water samples ranged between 2.6 – 4.2 NTU
for pond water and 5.0 – 7.0 NTU for laboratory water. Temperature is an important biologically significant
factor, which plays an important role in the metabolic activities of the organism. It also influences the metabolic
behavior of aquatic ecosystem [9]; [10]. The temperature was ranging from 25.40°C to 28.80°C during the study
period in pond water and 26.00°C to 30.00°C in lab water. Lowest water temperature (25.40°C) was observed in
December 2013 and highest temperature (30.00°C) value was observed in April and May 2013.
Table-1. Physico-chemical characteristics of pond water.
Period Odo Tur
NTU
Tem
o
C
pH DO
mg/l
TDS
mg/l
Sali.
mg/l
TA
mg/l
TH
mg/l
Ca
mg/l
Mg
mg/l
Na
mg/l
K
mg/l
July’13 U 3.2 27.5 7.4 5.9 280 282.5 560 275 61.4 80.2 45.7 5.6
Aug’13 U 4.0 26.4 7.1 6.2 320 294.2 488 320 67.5 99.5 40.8 6.3
Sept’13 U 2.8 26.8 7.7 6.8 270 267.9 519 240 71.2 84.7 35.4 5.8
Oct’13 U 2.6 27.1 7.5 5.8 360 250.8 399 310 64.3 72.1 39.6 5.0
Nov’13 U 3.1 26.0 7.2 6.4 345 269.1 423 280 60.9 86.9 43.3 6.1
Dec’13 U 3.4 25.4 7.8 6.0 310 257.5 572 295 63.1 64.8 37.5 5.3
Jan’14 U 2.8 26.2 7.4 5.8 285 274.6 496 260 59.0 85.6 43.2 5.9
Feb’14 U 2.6 25.8 7.9 5.4 270 298.3 534 270 58.9 80.9 37.4 6.2
Mar’14 U 3.5 27.3 7.2 6.5 325 283.7 401 300 64.2 71.4 44.6 5.4
Apr’14 U 3.1 28.6 7.5 5.3 350 251.9 578 275 69.7 69.8 40.7 4.9
May’14 U 4.2 28.8 7.8 5.9 310 282.3 594 265 63.7 94.2 44.8 5.6
Jun’14 U 3.3 27.4 7.3 6.2 280 250.6 428 310 68.4 98.5 41.7 6.2
[[[[[[[
pH is a term used universally to express the intensity of the acid or alkaline condition of a solution.
Most of the water samples are slightly alkaline due to presence of carbonates and bicarbonates. pH of bore water
influenced by geology of catchment area and the buffering capacity of water. The pH variation is mostly due to
diurnal interplay of photosynthesis and community respiration of the biota and also is one of the most important
single factors, which influences aquatic production [11]. The pH values of water samples varied between 7.1 to
7.9 and 6.8 to 7.2 respectively were found for pond and lab water. In the pond water the values were more than
7.0 and impart the alkaline nature where as in lab water the hydrogen ion concentration was in acidic condition
but within the limit prescribed. Dissolved Oxygen in water is essential for life. Deficiency of dissolved oxygen
gives bad odour to water due to anaerobic decomposition of organic waste [12]. Dissolved Oxygen in this study
ranges from 5.3 mg/l to 6.8 mg/l in pond water and 5.0 mg/l to 5.6 mg/l in the lab water. In any aquatic
ecosystem the level of dissolved oxygen depends on the factors like temperature of water, concentration of
dissolved solids and biological activity of all life. Total dissolved solids denote mainly the various kinds of
minerals available in the water. In natural waters dissolved solids are composed mainly of carbonates,
bicarbonates, chlorides, sulfates, phosphates, nitrate, calcium, magnesium, sodium, potassium and iron [13].
In the present investigation maximum value of total dissolved solids was recorded in October 2013
(360 mg/l) at pond water and minimum value of (155 mg/l) was observed in April 2014 at the laboratory water
sample. The chloride concentration or salinity serves as an indicator of pollution by sewage. People accustomed
to higher chloride in water are subjected to laxative effects [14]. In the present analysis, chloride concentration
was found in the range of 250.6 mg/l to 298.3 mg/l in pond water. In laboratory water the minimum quantity
was recorded as 114.8 mg/l in the month of March 2014 and the maximum of salinity was observed as 129.5
mg/l in the laboratory tap water in the month of July 2013. The pattern has also been recorded [15]; [16]. This
again implies that all the waters studied were all of satisfactory quality in terms of their chloride contents and
that chloride does not contribute to problems of taste in some of the waters. Alkalinity of water is its capacity to
neutralize a strong acid and it is normally due to the presence of bicarbonate, carbonate and hydroxide
compound of calcium, sodium and potassium [17]. Total alkalinity was recorded as the range between 399.00
mg/l to 594.00 mg/l for pond water and 42.00 mg/l to 93.00 mg/l for laboratory water.
Hardness is the property of water which prevents the lather formation with soap and increases the
boiling points of water. Hardness of water mainly depends upon the amount of calcium or magnesium salts or
both [17]. Total hardness values of pond and laboratory water were studied and they vary between 240.00 mg/l
to 320.00 mg/l and 69.00 mg/l to 98.00 mg/l respectively. Calcium is a soft gray alkaline earth metal which is
Physico-chemical Characteristics of Water Quality for Culturing the Freshwater Mussel…
DOI: 10.9790/3008-10621114 www.iosrjournals.org 13 | Page
directly related to hardness. Calcium is the most abundant substances of the natural water. In aquatic
environment calcium serves as one of the micronutrients for most of the organisms [18]. Calcium concentration
ranged between 58.90 mg/l to 71.20 mg/l in pond water and 20.80 mg/l to 42.70 mg/l in the laboratory water.
Magnesium is one of the most abundant elements in nature and it is a significant member in water
hardness, it gives an unpleasant taste to water. Magnesium content in the investigated water samples were
ranging from 64.80 mg/l to 99.50 mg/l for pond water and between 31.50 mg/l to 41.80 mg/l for the laboratory
water. Magnesium is essential for chlorophyll bearing organism, since it goes into composition of the pigments.
Decrease value of magnesium may be due to plankton and algal uptake [17].
Table-2. Physico-chemical characteristics of laboratory tap water.
Period Odo TurN
TU
Tem
o
C
pH DO
mg/l
TDS
mg/l
Sali.
mg/l
TA
mg/l
TH
mg/l
Ca
mg/l
Mg
mg/l
Na
mg/l
K
mg/l
July’13 U 5 28 7.0 5.1 195 129.5 60 98 28.4 38.4 18.6 3.2
Aug’13 U 4 26 6.8 5.6 192 118.4 54 87 34.9 34.6 14.9 2.8
Sept’13 U 4 26 7.1 5.2 164 115.7 42 93 42.7 32.0 22.7 2.6
Oct.’13 U 5 27 7.2 5.5 190 118.6 52 95 27.3 36.7 20.5 3.4
Nov.’13 U 3 28 7.0 5.3 160 115.7 85 84 20.8 39.2 17.8 3.9
Dec.’13 U 3 27 6.9 5.6 184 121.9 93 71 28.7 41.5 14.1 2.7
Jan.’14 U 5 28 7.1 5.0 178 124.8 84 83 26.3 35.8 21.5 1.9
Feb.’14 U 3 28 6.9 5.1 189 116.3 76 69 34.5 37.4 24.9 2.1
Mar.’14 U 4 29 6.8 5.4 167 114.8 72 82 30.9 31.5 18.7 3.6
Apr.’14 U 4 30 7.2 5.5 155 125.1 61 97 41.2 41.8 13.6 4.1
May’14 U 5 30 6.8 5.4 179 119.5 54 91 39.8 40.9 11.2 3.8
Jun.’14 U 4 28 7.1 5.3 183 123.2 47 86 35.1 37.5 24.8 2.9
The maximum value of sodium was recorded to be 45.70 mg/l in July month of 2013 at the water
sample of pond and minimum 11.20 mg/l in May 2014 at sample of laboratory water. The maximum value of
potassium was recorded to be 6.30 mg/l in August 2013 in pond water and the minimum value of 1.90 mg/l in
January 2014 in the laboratory tap water. Potassium plays a vital role in metabolism of fish environment and is
an important macronutrient. From the results obtained, it can be concluded that the K. Sathanur pond is a non-
polluted freshwater body due to the block of municipal sewage discharge. Higher amount of nutrients leads to
eutrophication. More nutrients in the water body and makes the water fit enough for the survival of aquatic
organisms. Due to high organic matter contamination hydrophytes are growing drastically and deposited into the
lake after death which consequently reducing the depth of the lake day by day. The results obtained from the
present investigation of pond as well as laboratory fresh water shall be useful in culturing the Freshwater
Mussel, L. marginalis It is also recommended that a periodical survey is essential to find out the water quality.
IV. Conclusion
The assessment of water quality is an important factor to assess of pollution levels. This study revealed
that the water in the studied area is suitable for drinking and domestic purposes including the cultural
performance of aquatic organisms at the physic-chemical level. The results were compared favourably with
standards.
Acknowledgement
The authors thanks the Principal, Periyar E.V.R College (Autonomous), Tiruchirappalli, Tamil Nadu,
India for the encouragement and providing facilities for the study.
References
[1] Beckerman A.P. (2014). What can modern statistical tools do for limnology? J. Limnol. 73 (Suppl.1): 161- 170,
[2] Alaka A. P. (2014). Limnological and Correlation Studies of Birnal Water Body of Sangli, Maharashtra Int. Res. J. Environment
Sci. Vol. 3 (9), 43-49.
[3] Basavaraja, Simpi, S. M., Hiremath, K. N. S. Murthy, K. N. Chandrashekarappa, Anil N. Patel, E.T.Puttiah, (2011), Analysis of
Water Quality Using Physico-Chemical Parameters Hosahalli Tank in Shimoga District.
[4] Kuzhali S. Surumbar, N. Manikandan and R. Kumuthakalavalli, (2012). Physico chemical and biological parameters of paper
industry effluent. Scholars Research Library. J. Nat. Prod. Plant Resour., 2 (3): 445- 448.
[5] Subha Rao, N.V. (1989). Hand book of Freshwater Molluscs of India, Zoological survey of India, Calcutta.
[6] Bauer, G. and Wachter, K. (2001). Ecology and Evolution of the Freshwater Mussels Unionida. Ecological studies, Vol. 145,
Springer : Berlin.
[7] Killen, I.J., Aldridge, D.C. and Oliver, P.G. (2004). Freshwater Bivalves of the BritishIslets. Occational Publication 82, Field
studies council and AIIDGAP : Present Mount Ford, UK.
[8] APHA (1986). Standard methods for the examination of water and wastewater 15th ed. APHA Washington D.C.
Physico-chemical Characteristics of Water Quality for Culturing the Freshwater Mussel…
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[9] Welch P.S. (1952) Limnology, McGraw Hill Book CO., New York, USA. 2nd Edition 538 pp.
[10] Alaka A. P. (2013). Limnological studies of perennial reservoirs of Khanapur tahsil of Sangli district, Maharashtra, J. Ecobiol. 32
(3), 185-196,
[11] Hora, S. L. and Pillay T.V.R. (1962). Hand book on fish culture in India-Pacific region, FAO Fish Biol.Tech. (14) 204 pp.
[12] Manivasakam,, N. (1980).. Physico-chemical examination of water, sewage and industrial effluents. India Pragati Prakashan,
Meerut
[13] Esmaeili H.R. and Johal M.S. (2005). Study of physico-chemical parameters of water of Gobind Sagar reservoir, India. Proc. Nat.
Sem. New Trends in Fishery Development in India. (Ed: M.S. Johal), Punjab University, Chandigarh, 173-177,
[14] Ramulu N. K. and Benarjee G. (2013); Physicochemical factors influenced plankton biodiversity and fishbundance- A case study
of Andhra Pradesh. Int. J. Lifesc. Bt. &Pharm. Res.
[15] Lendhe R.S. and Yeragi S.G. (2004). Seasional variation in primary productivity of Phirange Kharbav Lake,
[16] Chavan R.J., Sawat R.J., Himara C.J. and Tat M.B. (2004). Studies on water quality of Manjara Project
[17] Rath P., Bhatta D., Sahoo B.N. and Panda U.C. (2000). Multivariate statistical approach to study physic-chemical characteristics in
Nandira Brahmani River, Angul-Talcher Belt. Orissa, India. Poll. Res., 19 (4): 701- 710.
[18] Shah, I and Shah, H (2013). Physico-Chemical Dynamics in Littoral Zone of Nageen Basin of Dal Lake, Kashmir, India, Int. Res. J.
Environment Sci., 2 (3), 11-14.

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  • 1. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676. Volume 10, Issue 6 Ver. II (Nov - Dec. 2015), PP 11-14 www.iosrjournals.org DOI: 10.9790/3008-10621114 www.iosrjournals.org 11 | Page Physico-chemical Characteristics of Water Quality for Culturing the Freshwater Mussel Lamellidens marginalis in Pond and Laboratory Nirmala Natarajan1 and R. Susithira2 1 Associate Professor in Zoology, Periyar E.V.R. College (Autonomous), Tiruchirappalli - 620 023. 2 Teacher Educator, Urban Resource Center, SSA, Woraiyur, Tiruchirappalli - 620 003. Abstract: Physicochemical characteristics viz., turbidity, pH, temperature, dissolved oxygen, salinity, hardness and alkalinity of water were studied in the pond and laboratory for culturing the Freshwater Mussel Lamellidens marginalis. The optimal ranges for the survival of Mussels were detected for the culture maintenance. The data on the ecological factors of the pond and laboratory water have been studied for a period of one year (from July 2013 to June 2014) and discussed with the light of similar work. The findings of the present study confirmed that the physico-chemical parameters appeared to be varied due to the nature of water as well as season. Keywords: Freshwater mussel, Lamellidens marginalis, optimal ranges, physicochemical parameters. I. Introduction Water is a soul of nature which covers seven tenth of the earth’s surface. Water is an elixir of life, a precious gift of nature to mankind and millions of other species living on the earth. Water resources comprising of surface water viz., rivers, lakes and ponds, ground water, marine and coastal waters, support all living beings including human beings. India has vast fresh water resources in the form of both lentic and lotic ecosystems. The perennial reservoirs play an important role for domestic, agriculture and aquaculture as a valuable water resource. The lentic ecosystems have long attracted attention of ecologists, both for their importance as a source of drinking water and the development of fisheries [1]. Therefore, the attention is given on the physico-chemical factors which affect the aquatic inhabitants [2]. Wetlands are very productive ecosystems, which help in the regulation of biological cycles, maintenance of water quality, nutrient movement and support for food chains. In addition, they provide refuge for endangered species of plants and animals and economic benefits. The natural aquatic resources are causing heavy and varied pollution in aquatic environment leading to pollute water quality and depletion of aquatic biota [3]. Pollution refers to undesirable changes in the physical, chemical or biological characteristics of our environment. This has adversely affected the humans and other species of our biosphere directly or indirectly [4]. The Freshwater Molluscs constitute an important part of the ecosystem. Their participation in the way of life in many organisms has made them significant partners in the ecological communities [5]. Freshwater Mussels are widely distributed throughout the world occurring in both lotic and lentic systems [6]; [7]. In India , freshwater bodies show grate seasonal variations in the physicochemical factors which affect the natural populations of Freshwater Mussels and consequently, transmission of disease. In view of the above, the present study deals with the assessment of physic-chemical characteristics of a freshwater pond as well as the water present in the laboratory for the cultural and survival of the experimental animal Freshwater Mussel, L. marginalis. II. Materials and Methods The study was carried out in July 2013 to June 2014 in K. Sathanur, Moorthy Nagar pond located in Tiruchirappalli of Tamil Nadu and in the laboratory tap water. Water samples were collected for physico- chemical analyses in sterile bottles and taken to the laboratory aseptically. The collection was usually completed during morning hours between 6:00 a.m. to 10:00 a.m. For each sampling event, odour (Odo), turbidity (Tur), temperature (Tem), pH and dissolved oxygen (DO) were monitored at the sampling sites while total dissolved solids (TDS), salinity (Sali.), total hardness (TH), calcium (Ca), magnesium (Mg), sodium (Na) and potassium (K) were analyzed in the laboratory in accordance with the methods of FAO (1997) and APHA (1998).
  • 2. Physico-chemical Characteristics of Water Quality for Culturing the Freshwater Mussel… DOI: 10.9790/3008-10621114 www.iosrjournals.org 12 | Page III. Results and Discussion The Physico-chemical data of the pond water situated at K. sathanur and laboratory tap water for 13 physico-chemical parameters are presented in Table-1 and Table-2 respectively. The obtained results were compared with Standard prescribed under APHA [8]. The two water samples of pond and laboratory are colourless and also with no objectionable odour. The turbidity of water samples ranged between 2.6 – 4.2 NTU for pond water and 5.0 – 7.0 NTU for laboratory water. Temperature is an important biologically significant factor, which plays an important role in the metabolic activities of the organism. It also influences the metabolic behavior of aquatic ecosystem [9]; [10]. The temperature was ranging from 25.40°C to 28.80°C during the study period in pond water and 26.00°C to 30.00°C in lab water. Lowest water temperature (25.40°C) was observed in December 2013 and highest temperature (30.00°C) value was observed in April and May 2013. Table-1. Physico-chemical characteristics of pond water. Period Odo Tur NTU Tem o C pH DO mg/l TDS mg/l Sali. mg/l TA mg/l TH mg/l Ca mg/l Mg mg/l Na mg/l K mg/l July’13 U 3.2 27.5 7.4 5.9 280 282.5 560 275 61.4 80.2 45.7 5.6 Aug’13 U 4.0 26.4 7.1 6.2 320 294.2 488 320 67.5 99.5 40.8 6.3 Sept’13 U 2.8 26.8 7.7 6.8 270 267.9 519 240 71.2 84.7 35.4 5.8 Oct’13 U 2.6 27.1 7.5 5.8 360 250.8 399 310 64.3 72.1 39.6 5.0 Nov’13 U 3.1 26.0 7.2 6.4 345 269.1 423 280 60.9 86.9 43.3 6.1 Dec’13 U 3.4 25.4 7.8 6.0 310 257.5 572 295 63.1 64.8 37.5 5.3 Jan’14 U 2.8 26.2 7.4 5.8 285 274.6 496 260 59.0 85.6 43.2 5.9 Feb’14 U 2.6 25.8 7.9 5.4 270 298.3 534 270 58.9 80.9 37.4 6.2 Mar’14 U 3.5 27.3 7.2 6.5 325 283.7 401 300 64.2 71.4 44.6 5.4 Apr’14 U 3.1 28.6 7.5 5.3 350 251.9 578 275 69.7 69.8 40.7 4.9 May’14 U 4.2 28.8 7.8 5.9 310 282.3 594 265 63.7 94.2 44.8 5.6 Jun’14 U 3.3 27.4 7.3 6.2 280 250.6 428 310 68.4 98.5 41.7 6.2 [[[[[[[ pH is a term used universally to express the intensity of the acid or alkaline condition of a solution. Most of the water samples are slightly alkaline due to presence of carbonates and bicarbonates. pH of bore water influenced by geology of catchment area and the buffering capacity of water. The pH variation is mostly due to diurnal interplay of photosynthesis and community respiration of the biota and also is one of the most important single factors, which influences aquatic production [11]. The pH values of water samples varied between 7.1 to 7.9 and 6.8 to 7.2 respectively were found for pond and lab water. In the pond water the values were more than 7.0 and impart the alkaline nature where as in lab water the hydrogen ion concentration was in acidic condition but within the limit prescribed. Dissolved Oxygen in water is essential for life. Deficiency of dissolved oxygen gives bad odour to water due to anaerobic decomposition of organic waste [12]. Dissolved Oxygen in this study ranges from 5.3 mg/l to 6.8 mg/l in pond water and 5.0 mg/l to 5.6 mg/l in the lab water. In any aquatic ecosystem the level of dissolved oxygen depends on the factors like temperature of water, concentration of dissolved solids and biological activity of all life. Total dissolved solids denote mainly the various kinds of minerals available in the water. In natural waters dissolved solids are composed mainly of carbonates, bicarbonates, chlorides, sulfates, phosphates, nitrate, calcium, magnesium, sodium, potassium and iron [13]. In the present investigation maximum value of total dissolved solids was recorded in October 2013 (360 mg/l) at pond water and minimum value of (155 mg/l) was observed in April 2014 at the laboratory water sample. The chloride concentration or salinity serves as an indicator of pollution by sewage. People accustomed to higher chloride in water are subjected to laxative effects [14]. In the present analysis, chloride concentration was found in the range of 250.6 mg/l to 298.3 mg/l in pond water. In laboratory water the minimum quantity was recorded as 114.8 mg/l in the month of March 2014 and the maximum of salinity was observed as 129.5 mg/l in the laboratory tap water in the month of July 2013. The pattern has also been recorded [15]; [16]. This again implies that all the waters studied were all of satisfactory quality in terms of their chloride contents and that chloride does not contribute to problems of taste in some of the waters. Alkalinity of water is its capacity to neutralize a strong acid and it is normally due to the presence of bicarbonate, carbonate and hydroxide compound of calcium, sodium and potassium [17]. Total alkalinity was recorded as the range between 399.00 mg/l to 594.00 mg/l for pond water and 42.00 mg/l to 93.00 mg/l for laboratory water. Hardness is the property of water which prevents the lather formation with soap and increases the boiling points of water. Hardness of water mainly depends upon the amount of calcium or magnesium salts or both [17]. Total hardness values of pond and laboratory water were studied and they vary between 240.00 mg/l to 320.00 mg/l and 69.00 mg/l to 98.00 mg/l respectively. Calcium is a soft gray alkaline earth metal which is
  • 3. Physico-chemical Characteristics of Water Quality for Culturing the Freshwater Mussel… DOI: 10.9790/3008-10621114 www.iosrjournals.org 13 | Page directly related to hardness. Calcium is the most abundant substances of the natural water. In aquatic environment calcium serves as one of the micronutrients for most of the organisms [18]. Calcium concentration ranged between 58.90 mg/l to 71.20 mg/l in pond water and 20.80 mg/l to 42.70 mg/l in the laboratory water. Magnesium is one of the most abundant elements in nature and it is a significant member in water hardness, it gives an unpleasant taste to water. Magnesium content in the investigated water samples were ranging from 64.80 mg/l to 99.50 mg/l for pond water and between 31.50 mg/l to 41.80 mg/l for the laboratory water. Magnesium is essential for chlorophyll bearing organism, since it goes into composition of the pigments. Decrease value of magnesium may be due to plankton and algal uptake [17]. Table-2. Physico-chemical characteristics of laboratory tap water. Period Odo TurN TU Tem o C pH DO mg/l TDS mg/l Sali. mg/l TA mg/l TH mg/l Ca mg/l Mg mg/l Na mg/l K mg/l July’13 U 5 28 7.0 5.1 195 129.5 60 98 28.4 38.4 18.6 3.2 Aug’13 U 4 26 6.8 5.6 192 118.4 54 87 34.9 34.6 14.9 2.8 Sept’13 U 4 26 7.1 5.2 164 115.7 42 93 42.7 32.0 22.7 2.6 Oct.’13 U 5 27 7.2 5.5 190 118.6 52 95 27.3 36.7 20.5 3.4 Nov.’13 U 3 28 7.0 5.3 160 115.7 85 84 20.8 39.2 17.8 3.9 Dec.’13 U 3 27 6.9 5.6 184 121.9 93 71 28.7 41.5 14.1 2.7 Jan.’14 U 5 28 7.1 5.0 178 124.8 84 83 26.3 35.8 21.5 1.9 Feb.’14 U 3 28 6.9 5.1 189 116.3 76 69 34.5 37.4 24.9 2.1 Mar.’14 U 4 29 6.8 5.4 167 114.8 72 82 30.9 31.5 18.7 3.6 Apr.’14 U 4 30 7.2 5.5 155 125.1 61 97 41.2 41.8 13.6 4.1 May’14 U 5 30 6.8 5.4 179 119.5 54 91 39.8 40.9 11.2 3.8 Jun.’14 U 4 28 7.1 5.3 183 123.2 47 86 35.1 37.5 24.8 2.9 The maximum value of sodium was recorded to be 45.70 mg/l in July month of 2013 at the water sample of pond and minimum 11.20 mg/l in May 2014 at sample of laboratory water. The maximum value of potassium was recorded to be 6.30 mg/l in August 2013 in pond water and the minimum value of 1.90 mg/l in January 2014 in the laboratory tap water. Potassium plays a vital role in metabolism of fish environment and is an important macronutrient. From the results obtained, it can be concluded that the K. Sathanur pond is a non- polluted freshwater body due to the block of municipal sewage discharge. Higher amount of nutrients leads to eutrophication. More nutrients in the water body and makes the water fit enough for the survival of aquatic organisms. Due to high organic matter contamination hydrophytes are growing drastically and deposited into the lake after death which consequently reducing the depth of the lake day by day. The results obtained from the present investigation of pond as well as laboratory fresh water shall be useful in culturing the Freshwater Mussel, L. marginalis It is also recommended that a periodical survey is essential to find out the water quality. IV. Conclusion The assessment of water quality is an important factor to assess of pollution levels. This study revealed that the water in the studied area is suitable for drinking and domestic purposes including the cultural performance of aquatic organisms at the physic-chemical level. The results were compared favourably with standards. Acknowledgement The authors thanks the Principal, Periyar E.V.R College (Autonomous), Tiruchirappalli, Tamil Nadu, India for the encouragement and providing facilities for the study. References [1] Beckerman A.P. (2014). What can modern statistical tools do for limnology? J. Limnol. 73 (Suppl.1): 161- 170, [2] Alaka A. P. (2014). Limnological and Correlation Studies of Birnal Water Body of Sangli, Maharashtra Int. Res. J. Environment Sci. Vol. 3 (9), 43-49. [3] Basavaraja, Simpi, S. M., Hiremath, K. N. S. Murthy, K. N. Chandrashekarappa, Anil N. Patel, E.T.Puttiah, (2011), Analysis of Water Quality Using Physico-Chemical Parameters Hosahalli Tank in Shimoga District. [4] Kuzhali S. Surumbar, N. Manikandan and R. Kumuthakalavalli, (2012). Physico chemical and biological parameters of paper industry effluent. Scholars Research Library. J. Nat. Prod. Plant Resour., 2 (3): 445- 448. [5] Subha Rao, N.V. (1989). Hand book of Freshwater Molluscs of India, Zoological survey of India, Calcutta. [6] Bauer, G. and Wachter, K. (2001). Ecology and Evolution of the Freshwater Mussels Unionida. Ecological studies, Vol. 145, Springer : Berlin. [7] Killen, I.J., Aldridge, D.C. and Oliver, P.G. (2004). Freshwater Bivalves of the BritishIslets. Occational Publication 82, Field studies council and AIIDGAP : Present Mount Ford, UK. [8] APHA (1986). Standard methods for the examination of water and wastewater 15th ed. APHA Washington D.C.
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