This document analyzes the water quality of the Mula-Mutha River in Pune, India across four sampling stations from Khadakwasla Dam to Bund Garden. Water samples were collected during pre-monsoon, monsoon, and post-monsoon seasons and tested for pH, turbidity, hardness, dissolved oxygen, biochemical oxygen demand, and chemical oxygen demand. The results showed deteriorating water quality from upstream to downstream with pollution levels within satisfactory limits at Khadakwasla Dam but very poor quality at downstream stations. Major sources of river pollution identified were domestic sewage, industrial effluents, agricultural runoff, and waste discharged from temples. Future measures recommended included public awareness programs, industrial waste