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BIOELECTROCHEMISTRY
PAMELA LOBO
PSCH 101 (UNIT-4)
M.Sc. SEM-1 (2017-2018)
INTRODUCTION: -
• Electrochemistry study of ionic solutions and electrodes where ions
and electrons combine and separate.
• Biology is all about flesh and blood and bone.
• Becker in his book The Body Electric said-electrochemical events
going on in living systems.
• Bioelectrochemistry helps to uncover all undiscovered factsto open
our minds.
CELLS AND MEMBRANES: -
• Robert Hooke in 1665-honeycomblike structures called cells.
• Gordon and Grendell,1925-Cells contained in bags called
membranes of phospholipids.
mitochondrion
MEMBRANE POTENTIALS: -
• (Eg. of dead frog) what would happen if we apply electrical
stimulus to the nerve that feeds the frogs legs?
• How do neuron in a living organisms produce electrical signals?
• How does permeability work in resting potential?
• Permeability also comes in degrees.
RESTING MEMBRANE POTENTIAL: -
• Resting membrane potential comes from-uneven distribution of ions.
• Types of ions present: - cations-Na+ , K+and anions Cl-
• Potassium ions-higher conc. –inside the cell.
• Sodium and chloride ions –higher conc. –outside the cell.
HOW IONS CROSS THE MEMBRANE: -
If only K+ crosses the membrane: -
If only Na+ crosses the membrane: -
EQUILLIBRIUM POTENTIAL: -
Na+ K+ PUMPS: -
THEORY OF MEMBRANE POTENTIAL: -
• Descended from the concept of liquid junction potential.
• Some membranes affected by light-photo electrodes.
• Albert Szent-Gyorgyi-regarded as semiconductors.
• Also raised the idea of electron transfer.
• Jahn (1962)-bielectrode reactions(redox reactions)a
The difference in concentration are not the cause of potential. The
cause is the electron transfer reaction taking place at the two
interfaces.
INTERFACIAL ELECTRON TRANSFER IN
BIOLOGICAL SYSTEMS: -
• On the electrode side that is surface of protein is more complex than
that of a metal.
• The ions in solution for electron exchange are very large compared to
ions in electrochemistry.
ADSORPTION OF PROTEIN ONTO METALS FROM
SOLUTION: -
• Cytochrome c is the model protein used in surface studies.
• Szucs et al. (1991) experiment on adsorption of biomolecules on metal
surface.
• Gold electrode, solution of enzyme glucose oxidase.
• Voltagrams measured-(3 different stages)
• Szucs et al. proved that adsorption of enzyme on metal surface
maybe disastrous for the enzyme structure.
• Much information about protein absorption can be obtained by
measuring the electrochemical isotherm, the θ – c soln relation
at constant temperature for a series of fixed potentials.
• One takes the protein (P) as absorbing fast upon the metal, and
transferring n electrons, corresponding to n carbonyl
interactions. The dissociation reaction is taken to be rate
determining. Then (Roscoe, 1996):
REFERENCES:-
• Modern electrochemistry, Vol 2B, J.O.M Bokris and A.K.N
Reddy
• https://www.khanacademy.org/science/biology/human-
biology/neuron-nervous-system/a/the-membrane-potential
• https://www.sciencedirect.com/science/article/pii/S092777651
7302734

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Physical bioelectrochem presentation pamela

  • 1. BIOELECTROCHEMISTRY PAMELA LOBO PSCH 101 (UNIT-4) M.Sc. SEM-1 (2017-2018)
  • 2. INTRODUCTION: - • Electrochemistry study of ionic solutions and electrodes where ions and electrons combine and separate. • Biology is all about flesh and blood and bone. • Becker in his book The Body Electric said-electrochemical events going on in living systems. • Bioelectrochemistry helps to uncover all undiscovered factsto open our minds.
  • 3. CELLS AND MEMBRANES: - • Robert Hooke in 1665-honeycomblike structures called cells. • Gordon and Grendell,1925-Cells contained in bags called membranes of phospholipids. mitochondrion
  • 4. MEMBRANE POTENTIALS: - • (Eg. of dead frog) what would happen if we apply electrical stimulus to the nerve that feeds the frogs legs? • How do neuron in a living organisms produce electrical signals? • How does permeability work in resting potential? • Permeability also comes in degrees.
  • 6.
  • 7. • Resting membrane potential comes from-uneven distribution of ions. • Types of ions present: - cations-Na+ , K+and anions Cl- • Potassium ions-higher conc. –inside the cell. • Sodium and chloride ions –higher conc. –outside the cell.
  • 8.
  • 9. HOW IONS CROSS THE MEMBRANE: -
  • 10. If only K+ crosses the membrane: -
  • 11. If only Na+ crosses the membrane: -
  • 14. THEORY OF MEMBRANE POTENTIAL: - • Descended from the concept of liquid junction potential. • Some membranes affected by light-photo electrodes. • Albert Szent-Gyorgyi-regarded as semiconductors. • Also raised the idea of electron transfer. • Jahn (1962)-bielectrode reactions(redox reactions)a
  • 15. The difference in concentration are not the cause of potential. The cause is the electron transfer reaction taking place at the two interfaces.
  • 16. INTERFACIAL ELECTRON TRANSFER IN BIOLOGICAL SYSTEMS: - • On the electrode side that is surface of protein is more complex than that of a metal. • The ions in solution for electron exchange are very large compared to ions in electrochemistry.
  • 17. ADSORPTION OF PROTEIN ONTO METALS FROM SOLUTION: - • Cytochrome c is the model protein used in surface studies. • Szucs et al. (1991) experiment on adsorption of biomolecules on metal surface. • Gold electrode, solution of enzyme glucose oxidase. • Voltagrams measured-(3 different stages)
  • 18. • Szucs et al. proved that adsorption of enzyme on metal surface maybe disastrous for the enzyme structure. • Much information about protein absorption can be obtained by measuring the electrochemical isotherm, the θ – c soln relation at constant temperature for a series of fixed potentials. • One takes the protein (P) as absorbing fast upon the metal, and transferring n electrons, corresponding to n carbonyl interactions. The dissociation reaction is taken to be rate determining. Then (Roscoe, 1996):
  • 19.
  • 20. REFERENCES:- • Modern electrochemistry, Vol 2B, J.O.M Bokris and A.K.N Reddy • https://www.khanacademy.org/science/biology/human- biology/neuron-nervous-system/a/the-membrane-potential • https://www.sciencedirect.com/science/article/pii/S092777651 7302734