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Pharmaceuticals in the Environment.
By:Abdel-Rahman Ragab
Outlines.
1- why interests increased for presence of pharmaceuticals in
the environment ?
2- Pathways for entry into the environment.
3- Environmental detection of pharmaceuticals.
Part 1 : why interests
increased for presence
of pharmaceuticals in
the environment ?
Vet diclofenac catastrophe.
In 1980s there were up to 80 million eagles in India; but today
the population numbers only several thousand.
99% decline of eagles species in south Asia.
Effects on aquatic organisms.
1-tricyclic antidepressants (ppb levels) : affect on development
,spawning and behaviors in shellfish, ciliates and other aquatic
organisms.
2-calcium-channel blockers : affect on sperm activity of some
aquatic.
3- Antiepileptic drugs : trigger extensive apoptosis in the
developing brain of human  neurodegeneration.
4-ppm and sub-ppm levels of various drugs (NSAIDS,
glucocorticoids, anti-fibrotics) affect collagen metabolism in
teleost fish, leading to defective/blocked fin regeneration.
5- Multi-drug transporters (efflux pumps) are common
defensive strategies for aquatic biota — possible significance of
efflux pump inhibitors in compromising aquatic health?
Drugs Having Double Uses:
Medicinals and Pest-Control Agents
It poses the possibility that these alternative uses serve as
additional sources for their introduction to
the environment.
Examples:(4-aminopyridine,
warfarin,triclosan,azacholesterols,antibiotics
,acetaminophen,caffeine,NSAIDs and pentobarbital).
Acetaminophen : analgesic and useful for control brown
Tree snake
Antibiotics and endocrine disruptors.
-antibiotics cause bacterial resistance .
-endocrine disruptors : interfere with hormone and disrupt its
function ex: estrogen in contraceptive pill.
A total of US$954 billion was spent on prescription medications globally in
2011 alone. in 2002 95 contaminant measured in us.
94cg
Figure 1 Number of publications per year for the period 1996–2014 retrieved
using Web of Knowledge™ and the search words “pharmaceutical*” and “waste
water.”
Part 2: Pathways for
entry into the
environment.
Pathways to sewage.
Wastes of
Pharmaceutical
manufacture
sewage
Urine &
feces
bathing
disposing
Pathways to Air .
Non inhaled active
ingredient
insignificant
completely
oxidized to innocuous
compounds such as
carbon dioxide and water
Pathways to soil.
Urine &
feces
sewage
disposing
into the waste
bin
Wastes of
Pharmaceutical
manufacture
River/Estuarine/Coastal
water
Pathway to water.
Surface water
Sewage treatment
River/Estuarine/Coastal water
Ground water
Landfill
Pathway to water
River/Estuarine/Coastal
water
Sewage treatment
Soil
Surface water
swimming
Pathway to biota.
River/Estuarine/Coastal
water
Groundwater Drinking water
sources
Figure 2 Pathways for entry into the environment.
Part 3: Environmental
detection of
pharmaceuticals.
Figure 2: Procedures and techniques used in the preparation and analysis of
environmental samples.
Sample collection.
Solid sludge.
collection
Placed in glass bottle then freeze-dried and ground
to 250 µm sized particle before storage .
Sample collection.
-composite sample, grab sampler,24-h sample with weather
consideration ( rain and seasonal variation).
bottle types used for the collection
of water Samples.
-Type chosen according to analyte of interest and environmental sample .
-silanized or not (alkoxysilane molecules).
-recovery % & physicochemical properties important .
Amber Clear glass PET HDPE
Sample bottle types used for the collection of water
samples in some field investigations of pharmaceutical analytes
Sample storage.
Purpose :prevention bacterial growth & analyte degradation.
When direct analysis isn't available .
Preservative are added ex HCL,HNO3 and H2SO4.
72 h store at 4 °C without preservative.
3 weeks at 4 °C 24 h then frozen at -20 °C for 3 weeks
Sample filtration.
Purpose : to remove particulate matter with filter paper.
Type of filter paper depend on analyte of interest
Ex estrogen glass fiber filters
Extraction.
Purpose : increase conc of analyte to reach detection limit of device .
2 types Solid-phase extraction & Liquid–liquid extraction.
Solid –phase extraction is suitable .
Separtion.
By high performance (pressure) liquid chromatography (HPLC)
Mass spectrometric detection and
quantization
Pharmaceuticals in the environment
Pharmaceuticals in the environment
Pharmaceuticals in the environment

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Pharmaceuticals in the environment

  • 1. Pharmaceuticals in the Environment. By:Abdel-Rahman Ragab
  • 2. Outlines. 1- why interests increased for presence of pharmaceuticals in the environment ? 2- Pathways for entry into the environment. 3- Environmental detection of pharmaceuticals.
  • 3. Part 1 : why interests increased for presence of pharmaceuticals in the environment ?
  • 4. Vet diclofenac catastrophe. In 1980s there were up to 80 million eagles in India; but today the population numbers only several thousand. 99% decline of eagles species in south Asia.
  • 5.
  • 6. Effects on aquatic organisms. 1-tricyclic antidepressants (ppb levels) : affect on development ,spawning and behaviors in shellfish, ciliates and other aquatic organisms. 2-calcium-channel blockers : affect on sperm activity of some aquatic. 3- Antiepileptic drugs : trigger extensive apoptosis in the developing brain of human  neurodegeneration. 4-ppm and sub-ppm levels of various drugs (NSAIDS, glucocorticoids, anti-fibrotics) affect collagen metabolism in teleost fish, leading to defective/blocked fin regeneration. 5- Multi-drug transporters (efflux pumps) are common defensive strategies for aquatic biota — possible significance of efflux pump inhibitors in compromising aquatic health?
  • 7.
  • 8. Drugs Having Double Uses: Medicinals and Pest-Control Agents It poses the possibility that these alternative uses serve as additional sources for their introduction to the environment. Examples:(4-aminopyridine, warfarin,triclosan,azacholesterols,antibiotics ,acetaminophen,caffeine,NSAIDs and pentobarbital). Acetaminophen : analgesic and useful for control brown Tree snake
  • 9. Antibiotics and endocrine disruptors. -antibiotics cause bacterial resistance . -endocrine disruptors : interfere with hormone and disrupt its function ex: estrogen in contraceptive pill.
  • 10. A total of US$954 billion was spent on prescription medications globally in 2011 alone. in 2002 95 contaminant measured in us. 94cg Figure 1 Number of publications per year for the period 1996–2014 retrieved using Web of Knowledge™ and the search words “pharmaceutical*” and “waste water.”
  • 11. Part 2: Pathways for entry into the environment.
  • 12.
  • 13. Pathways to sewage. Wastes of Pharmaceutical manufacture sewage Urine & feces bathing disposing
  • 14. Pathways to Air . Non inhaled active ingredient insignificant completely oxidized to innocuous compounds such as carbon dioxide and water
  • 15. Pathways to soil. Urine & feces sewage disposing into the waste bin Wastes of Pharmaceutical manufacture River/Estuarine/Coastal water
  • 16. Pathway to water. Surface water Sewage treatment River/Estuarine/Coastal water Ground water Landfill
  • 17. Pathway to water River/Estuarine/Coastal water Sewage treatment Soil Surface water swimming
  • 19. Figure 2 Pathways for entry into the environment.
  • 20.
  • 21. Part 3: Environmental detection of pharmaceuticals.
  • 22. Figure 2: Procedures and techniques used in the preparation and analysis of environmental samples.
  • 23. Sample collection. Solid sludge. collection Placed in glass bottle then freeze-dried and ground to 250 µm sized particle before storage .
  • 24. Sample collection. -composite sample, grab sampler,24-h sample with weather consideration ( rain and seasonal variation).
  • 25. bottle types used for the collection of water Samples. -Type chosen according to analyte of interest and environmental sample . -silanized or not (alkoxysilane molecules). -recovery % & physicochemical properties important . Amber Clear glass PET HDPE
  • 26. Sample bottle types used for the collection of water samples in some field investigations of pharmaceutical analytes
  • 27. Sample storage. Purpose :prevention bacterial growth & analyte degradation. When direct analysis isn't available . Preservative are added ex HCL,HNO3 and H2SO4. 72 h store at 4 °C without preservative. 3 weeks at 4 °C 24 h then frozen at -20 °C for 3 weeks
  • 28. Sample filtration. Purpose : to remove particulate matter with filter paper. Type of filter paper depend on analyte of interest Ex estrogen glass fiber filters
  • 29. Extraction. Purpose : increase conc of analyte to reach detection limit of device . 2 types Solid-phase extraction & Liquid–liquid extraction. Solid –phase extraction is suitable .
  • 30. Separtion. By high performance (pressure) liquid chromatography (HPLC)
  • 31. Mass spectrometric detection and quantization