The document describes experiments conducted by students to isolate bacteriophages from environmental samples. They enriched soil samples for phage particles, purified isolated phages through multiple rounds of plating and titration, and increased phage concentration through lysing infected bacterial cells. The students then isolated and analyzed phage DNA through spectrophotometry and restriction enzyme digestion followed by gel electrophoresis. While they were unable to find novel phages, the experiments provided experience in bacteriophage isolation techniques.
Since no universal tissue preparation method will be ideal for all sample and tissue types, the IHC protocol given here is intended as a starting point from which the experimenter should optimize as needed.
This guide is key to successful IHC experiments. Since no universal tissue preparation method will be ideal for all sample and tissue types, all protocols given here are intended as a starting point from which the experimenter must optimize as needed.
Since no universal tissue preparation method will be ideal for all sample and tissue types, the IHC protocol given here is intended as a starting point from which the experimenter should optimize as needed.
This guide is key to successful IHC experiments. Since no universal tissue preparation method will be ideal for all sample and tissue types, all protocols given here are intended as a starting point from which the experimenter must optimize as needed.
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. / NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Anti-Cleaved-PARP-1 (D214) -http://www.stjohnslabs.com/cleaved-parp-1-d214-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate
Anti-HSP A5 -http://www.stjohnslabs.com/hsp-a5-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Anti-Bad -http://www.stjohnslabs.com/bad-antibody-p-91313
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins[3][4] that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 42 kDa.
Anti-β-actin -http://www.stjohnslabs.com/b-actin-antibody-p-98565
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).
Anti-N-cadherin -http://www.stjohnslabs.com/n-cadherin-antibody-p-93283
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade.
Anti-EGFR -http://www.stjohnslabs.com/egfr-antibody-p-92140
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Anti-Bax -http://www.stjohnslabs.com/bax-antibody-p-91328
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Anti-MIF -http://www.stjohnslabs.com/mif-antibody-p-93124
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Western Blot Antibody Customer Review for Anti-Endoplasmin Polyclonal Antibod...St John's Laboratory Ltd
The Endoplasmin protein (also known as HSP90B1) is a molecular chaperone encoded by the HSP90B1 gene in humans. It plays a role in the processing and transport of proteins such as integrins and Toll-like receptors. It is located in the endoplasmic reticulum. The Endoplasmin polyclonal antibody detects endogenous levels of Endoplasmin protein.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Many of these processes are mediated by extensive and intimate interactions of actin with cellular membranes.
Anti-Actin β -http://www.stjohnslabs.com/actin-b-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Anti-Dnmt3b -http://www.stjohnslabs.com/dnmt3b-antibody-p-92052
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation.
Anti-Lamin A/C -http://www.stjohnslabs.com/lamin-ac-antibody-p-92942
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. / NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Anti-Cleaved-PARP-1 (D214) -http://www.stjohnslabs.com/cleaved-parp-1-d214-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate
Anti-HSP A5 -http://www.stjohnslabs.com/hsp-a5-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Anti-Bad -http://www.stjohnslabs.com/bad-antibody-p-91313
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins[3][4] that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Its molecular weight is approximately 42 kDa.
Anti-β-actin -http://www.stjohnslabs.com/b-actin-antibody-p-98565
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).
Anti-N-cadherin -http://www.stjohnslabs.com/n-cadherin-antibody-p-93283
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade.
Anti-EGFR -http://www.stjohnslabs.com/egfr-antibody-p-92140
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Anti-Bax -http://www.stjohnslabs.com/bax-antibody-p-91328
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
Anti-MIF -http://www.stjohnslabs.com/mif-antibody-p-93124
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Western Blot Antibody Customer Review for Anti-Endoplasmin Polyclonal Antibod...St John's Laboratory Ltd
The Endoplasmin protein (also known as HSP90B1) is a molecular chaperone encoded by the HSP90B1 gene in humans. It plays a role in the processing and transport of proteins such as integrins and Toll-like receptors. It is located in the endoplasmic reticulum. The Endoplasmin polyclonal antibody detects endogenous levels of Endoplasmin protein.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Many of these processes are mediated by extensive and intimate interactions of actin with cellular membranes.
Anti-Actin β -http://www.stjohnslabs.com/actin-b-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Anti-Dnmt3b -http://www.stjohnslabs.com/dnmt3b-antibody-p-92052
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation.
Anti-Lamin A/C -http://www.stjohnslabs.com/lamin-ac-antibody-p-92942
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Det är glädjande många pensionärer som går och funderar på att starta eget. Låt oss vara realister – att starta eget innebär många både för- och nackdelar. Den här bildserien tar upp de viktigaste, så att du får möjlighet att tänka igenom olika aspekter innan du sätter igång. Innehållet i denna bildserie är hämtat från boken "Starta eget företag snabbt och lönsamt". Den boken kan du köpa från sajten http://tips-om.se eller nätbokhandlarna Adlibris, Bokus och Bokia.
2. Vill du bli din egen chef? Innehållet i denna bildserie är hämtat från boken "Starta eget företag snabbt och lönsamt". Den boken kan du köpa från sajten http://tips-om.se eller nätbokhandlarna Adlibris, Bokus och Bokia.
3. Du kan styra över allt. Du fattar alla beslut – åtminstone i början innan företaget växer. Allt blir beroende av dina beslut och det kommer kännas enormt tillfredsställande om du lyckas. Innehållet i denna bildserie är hämtat från boken "Starta eget företag snabbt och lönsamt". Den boken kan du köpa från sajten http://tips-om.se eller nätbokhandlarna Adlibris, Bokus och Bokia.
4. Gillar du att ha stor frihet och göra mycket som du vill? Innehållet i denna bildserie är hämtat från boken "Starta eget företag snabbt och lönsamt". Den boken kan du köpa från sajten http://tips-om.se eller nätbokhandlarna Adlibris, Bokus och Bokia.
5. Du kan oftast välja när och var du själv vill jobba. Jag säger inte att du kommer jobba mindre – snarare mer. Det finns ett gammalt uttryck ungefär så här: ”En egenföretagare är en person som hellre jobbar 80 timmar i veckan för sitt eget företag än jobbar 40 timmar för någon annan”. Innehållet i denna bildserie är hämtat från boken "Starta eget företag snabbt och lönsamt". Den boken kan du köpa från sajten http://tips-om.se eller nätbokhandlarna Adlibris, Bokus och Bokia.
6. Du kanske gillar att ha mycket direktkontakt med dina kunder? Innehållet i denna bildserie är hämtat från boken "Starta eget företag snabbt och lönsamt". Den boken kan du köpa från sajten http://tips-om.se eller nätbokhandlarna Adlibris, Bokus och Bokia.
7. Som egenföretagare får du betydigt fler och närmare kontakter med kunder, tillverkare, leverantörer och andra intressenter. Innehållet i denna bildserie är hämtat från boken "Starta eget företag snabbt och lönsamt". Den boken kan du köpa från sajten http://tips-om.se eller nätbokhandlarna Adlibris, Bokus och Bokia.
8. Vill du ha möjlighet att tjäna mer än vad du gör i dag? Innehållet i denna bildserie är hämtat från boken "Starta eget företag snabbt och lönsamt". Den boken kan du köpa från sajten http://tips-om.se eller nätbokhandlarna Adlibris, Bokus och Bokia.
9. Du påverkar själv hur mycket du tjänar och lyckas du bra så kommer du känna en enorm tillfredsställelse – inte bara för att du tjänar mer pengar utan för att det är DU som åstadkommit detta. Innehållet i denna bildserie är hämtat från boken "Starta eget företag snabbt och lönsamt".
Monte Anderson of Options Real Estate explains how we can rediscover DUNCANVILLE. The idea is to take a different look at the City of Duncanville in order to see the opportunities. Duncanville has the opportunity to become an urban based community where there is an abundance of local entrepreneurs and walkable/bikeable streets.
This presentation is about quality assessment of soil and wastewater sample by various parameters. All the aspects of assessment from sampling to analysis are described in this presentation
polymerase chain reaction work-flow by Dr kelvin Agimogim.pptxkelvinagimogim1
Embark on a PCR expedition! Explore the fascinating journey through the Polymerase Chain Reaction (PCR) workflow, where science seamlessly blends with precision. Unveil the mysteries of PCR, from DNA discovery to mastering amplification, presented with clarity and a touch of flair. Prepare to immerse yourself in the realm of genetic innovation, elevating your understanding of this revolutionary technique. Join us as we amplify the excitement together!
RNA, DNA Isolation and cDNA synthesis.pptxASJADRAZA10
Isolation, quantification of nucleic acids from wheat and synthesis of cDNA.
Introduction
List of Genotypes
DNA Isolation (CTAB method)
Qualitative check of DNA- Gel electrophoresis
Quantitative test of DNA- Spectrophotometer
Protocol for RNA Isolation
RNA Confirmation
Normalization of RNA
cDNA Synthesis
Protocol for DNA Isolation of plant
50-100mg (2-3) young leaves were collected, then washed with tap water followed by distilled water in petri dish.
Leaves were ground using ethanol sterilized mortar pestle for 15-20 sec, by taking 1mL extraction buffer.
1mL (1000μL) of extraction buffer was again added to collect paste from mortar pestle & then transferred to the 2 mL micro centrifuge tube.
The sample in the tube is incubated at 65°C in water bath for 35-45 mins. (Contents in the tube was mixed by inverting at an interval for 5-10 mins)
The tubes were cooled for 10 minutes in ice.
The sample of equal vol (2mL) was centrifuged @14,000 rpm for 10 mins.
After that the supernatant was transferred to new 2 mL centrifuge tube and equal volume (as of sample) of chloroform: Isoamyl alcohol (24:1) was added.
Then mixed gently for 5-7 mins by inverting the tubes.
Again centrifuged for 10 mins @10,000 rpm
After centrifugation, three layers were observed in the tube.
a) aqueous phase i.e. DNA+RNA
b) protein coagulate
c) organic phase i.e. Chloroform
Again the supernatant (aqueous phase) was collected in 1.5mL tube and equal volume of ice-cold isopropanol was added and stored in -20°C overnight.
Following day, tubes were again centrifuged @10,000rpm for 10 mins.
The supernatant was discarded without disturbing the DNA pellet.
70% ethanol is taken and 0.5mL of it was added to the sample and mixed by tapping for 5 mins.
Again centrifuged @10,000rpm for 10 mins and the supernatant was discarded.
Pellet (DNA Precipitate) was air dried for 10 mins.
Then dissolved in 50μL TE-1X Buffer and the sample was stored at -20°C.
1g of analytical grade Agarose was weighed.
100 mL of autoclaved 1X TBE was added in flask.
Now heated on the oven until the solution becomes transparent.
Solution was allowed to cool down to 60℃.
2 μL of Ethidium Bromide (EtBr) is added in the flask.
Melted agarose gel was poured into the casting tray along with comb.
Any bubble in the gel was removed.
After solidification of gel, comb was removed gently and then running buffer was added in the electrophoretic tank.
Once gel got solidified, it was transferred it into gel tank.
A parafilm was taken and on it 2μL loading dye and 3μL sample was taken, gently mixed with the pipette tip only.
Then the mixture (sample +loading dye) was loaded into the well.
Then electrophoretic unit was run at 90 volt for 50-55 mins.
After that gel was put into the Gel Doc to see the DNA band
(using UV light).
Bright colour band were observed as in the figure.
Few (100-150mg) young leaves were ground into fine powder using liquid Nitrogen.
Induction of transformation by a deoxyribonucleic acid fraction isolated from...Babita Neupane
This is a highlight of the one of the groundbreaking paper of early 1940's in field of molecular biology.
Induction of transformation by a deoxyribonucleic acid fraction isolated from Pneumococcus Type III. Avery, O.T., Macleod, C. M., McCarty, M. (1944) The journal of experimental medicine 79(2):137-58.
Sudheer Mechineni, Head of Application Frameworks, Standard Chartered Bank
Discover how Standard Chartered Bank harnessed the power of Neo4j to transform complex data access challenges into a dynamic, scalable graph database solution. This keynote will cover their journey from initial adoption to deploying a fully automated, enterprise-grade causal cluster, highlighting key strategies for modelling organisational changes and ensuring robust disaster recovery. Learn how these innovations have not only enhanced Standard Chartered Bank’s data infrastructure but also positioned them as pioneers in the banking sector’s adoption of graph technology.
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdfMalak Abu Hammad
Discover how MongoDB Atlas and vector search technology can revolutionize your application's search capabilities. This comprehensive presentation covers:
* What is Vector Search?
* Importance and benefits of vector search
* Practical use cases across various industries
* Step-by-step implementation guide
* Live demos with code snippets
* Enhancing LLM capabilities with vector search
* Best practices and optimization strategies
Perfect for developers, AI enthusiasts, and tech leaders. Learn how to leverage MongoDB Atlas to deliver highly relevant, context-aware search results, transforming your data retrieval process. Stay ahead in tech innovation and maximize the potential of your applications.
#MongoDB #VectorSearch #AI #SemanticSearch #TechInnovation #DataScience #LLM #MachineLearning #SearchTechnology
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!SOFTTECHHUB
As the digital landscape continually evolves, operating systems play a critical role in shaping user experiences and productivity. The launch of Nitrux Linux 3.5.0 marks a significant milestone, offering a robust alternative to traditional systems such as Windows 11. This article delves into the essence of Nitrux Linux 3.5.0, exploring its unique features, advantages, and how it stands as a compelling choice for both casual users and tech enthusiasts.
zkStudyClub - Reef: Fast Succinct Non-Interactive Zero-Knowledge Regex ProofsAlex Pruden
This paper presents Reef, a system for generating publicly verifiable succinct non-interactive zero-knowledge proofs that a committed document matches or does not match a regular expression. We describe applications such as proving the strength of passwords, the provenance of email despite redactions, the validity of oblivious DNS queries, and the existence of mutations in DNA. Reef supports the Perl Compatible Regular Expression syntax, including wildcards, alternation, ranges, capture groups, Kleene star, negations, and lookarounds. Reef introduces a new type of automata, Skipping Alternating Finite Automata (SAFA), that skips irrelevant parts of a document when producing proofs without undermining soundness, and instantiates SAFA with a lookup argument. Our experimental evaluation confirms that Reef can generate proofs for documents with 32M characters; the proofs are small and cheap to verify (under a second).
Paper: https://eprint.iacr.org/2023/1886
In his public lecture, Christian Timmerer provides insights into the fascinating history of video streaming, starting from its humble beginnings before YouTube to the groundbreaking technologies that now dominate platforms like Netflix and ORF ON. Timmerer also presents provocative contributions of his own that have significantly influenced the industry. He concludes by looking at future challenges and invites the audience to join in a discussion.
Removing Uninteresting Bytes in Software FuzzingAftab Hussain
Imagine a world where software fuzzing, the process of mutating bytes in test seeds to uncover hidden and erroneous program behaviors, becomes faster and more effective. A lot depends on the initial seeds, which can significantly dictate the trajectory of a fuzzing campaign, particularly in terms of how long it takes to uncover interesting behaviour in your code. We introduce DIAR, a technique designed to speedup fuzzing campaigns by pinpointing and eliminating those uninteresting bytes in the seeds. Picture this: instead of wasting valuable resources on meaningless mutations in large, bloated seeds, DIAR removes the unnecessary bytes, streamlining the entire process.
In this work, we equipped AFL, a popular fuzzer, with DIAR and examined two critical Linux libraries -- Libxml's xmllint, a tool for parsing xml documents, and Binutil's readelf, an essential debugging and security analysis command-line tool used to display detailed information about ELF (Executable and Linkable Format). Our preliminary results show that AFL+DIAR does not only discover new paths more quickly but also achieves higher coverage overall. This work thus showcases how starting with lean and optimized seeds can lead to faster, more comprehensive fuzzing campaigns -- and DIAR helps you find such seeds.
- These are slides of the talk given at IEEE International Conference on Software Testing Verification and Validation Workshop, ICSTW 2022.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Pushing the limits of ePRTC: 100ns holdover for 100 daysAdtran
At WSTS 2024, Alon Stern explored the topic of parametric holdover and explained how recent research findings can be implemented in real-world PNT networks to achieve 100 nanoseconds of accuracy for up to 100 days.
UiPath Test Automation using UiPath Test Suite series, part 6DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 6. In this session, we will cover Test Automation with generative AI and Open AI.
UiPath Test Automation with generative AI and Open AI webinar offers an in-depth exploration of leveraging cutting-edge technologies for test automation within the UiPath platform. Attendees will delve into the integration of generative AI, a test automation solution, with Open AI advanced natural language processing capabilities.
Throughout the session, participants will discover how this synergy empowers testers to automate repetitive tasks, enhance testing accuracy, and expedite the software testing life cycle. Topics covered include the seamless integration process, practical use cases, and the benefits of harnessing AI-driven automation for UiPath testing initiatives. By attending this webinar, testers, and automation professionals can gain valuable insights into harnessing the power of AI to optimize their test automation workflows within the UiPath ecosystem, ultimately driving efficiency and quality in software development processes.
What will you get from this session?
1. Insights into integrating generative AI.
2. Understanding how this integration enhances test automation within the UiPath platform
3. Practical demonstrations
4. Exploration of real-world use cases illustrating the benefits of AI-driven test automation for UiPath
Topics covered:
What is generative AI
Test Automation with generative AI and Open AI.
UiPath integration with generative AI
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
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Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...SOFTTECHHUB
The choice of an operating system plays a pivotal role in shaping our computing experience. For decades, Microsoft's Windows has dominated the market, offering a familiar and widely adopted platform for personal and professional use. However, as technological advancements continue to push the boundaries of innovation, alternative operating systems have emerged, challenging the status quo and offering users a fresh perspective on computing.
One such alternative that has garnered significant attention and acclaim is Nitrux Linux 3.5.0, a sleek, powerful, and user-friendly Linux distribution that promises to redefine the way we interact with our devices. With its focus on performance, security, and customization, Nitrux Linux presents a compelling case for those seeking to break free from the constraints of proprietary software and embrace the freedom and flexibility of open-source computing.
Generative AI Deep Dive: Advancing from Proof of Concept to ProductionAggregage
Join Maher Hanafi, VP of Engineering at Betterworks, in this new session where he'll share a practical framework to transform Gen AI prototypes into impactful products! He'll delve into the complexities of data collection and management, model selection and optimization, and ensuring security, scalability, and responsible use.
2. What is a Bacteriophage?
• A virus that parasitizes a bacterium by infecting it
and reproducing inside it
• Bacteriophage means “eater of bacteria”
• There are an estimated 1031 worldwide, they are
everywhere *from the seas to the soil we walk on*
• One of the most diverse things on the planet
3. How are they useful?
• They destroy up to 40% of the bacteria in Earth’s
oceans each day.
• The can affect bacteria which can be good and bad
4. What are we trying to do?
• We are trying to isolate a new strand of arthobacter
phage by taking samples from the environment
around us.
• This could provide a framework for SEA scientists
and other researchers to delve into the possible
utility of these organisms in a variety of biomedical,
heath, environmental and ecological applications.
5. Methods
• Collected our soil samples
• Enriched our sample by adding sterile water, sterile 10 x LB medium, and
1 M CaCl solution then we incubated at 37 degrees for 24-48 hours.
• We then used this solution and put it in a conical tube, spun it for 2000
rpm for 5 min, then we pour the supernatant into a filtered syringe and
pushed what we could through.
• We then used 100 ml solution to make a titration out to 10-4
• Using the titrations we made plates
• Incubated at 30 degrees C for 24-48 hours
6. Methods (continued)
• Most of the phage that we got did not yield plaques so we used the Hudson
phage instead (KK 09-17-2013, Enrich 10-3 Hudson – MacKenzie, KK 07-172013, Enrich 10-3 Hudson – Sullivan)
• Using an inoculating loop we picked a plaque then put it into a
microcentifuge tube with PB and then we preformed the titration process
out to -5
• Then using the titrations we plated it out again with the mixture of our
phage, host bacteria and TA and incubated at 30 degrees C for 24-48 hours
• We also tried to re-enrich our samples and did a spot test to try and get a
plaque to see if we had phage, which didn’t work
7. Methods (continued)
• We then made a streak plate with the Hudson phage using an inoculating
loop we picked a plaque and streaked it across a plate and poured a TA
and arthro mixture onto the least concentrated section first and swirled it
carefully from the least concentrated section to the most concentrated
section to reduce contamination
• We did a spot test of the new enrichment with yielded nothing
• We then purified our phage three times using the titration process
•
•
•
•
•
We picked a plaque
Put it into a microcenrifuge tube with PB
(MacKenzie) tittered it out to 10-2 every time , (Sullivan) tittered it out to -4, -3, then -2
Placed it in a corresponding labeled host bacteria and let sit for 15 min
Added TA and poured them onto plates
8. Methods (continued)
• Using a webbed plate we added 8ml of PB onto it and let sit for an hour
• We then filtered the PB/phage solution using a filter syringe
• Then using that we titrated out to 10-10
• And using the titrations we plated all of them out with the phage/host
bacteria/TA mixture
• Also using the titration we did a spot test where we put 5 ml of each
titration onto a different square
• Then we incubated them at 30 degrees C
9. Methods (continued)
• Using the titration number that was best webbed plate we took our MTL
and titrated it out to that number again.
• Then using that number we took that titration and plated out 10 plates
(using the phage/bacteria/TA mixture) and incubated at 30 degrees C
• This then yielded 10 webbed plates which we flooded with PB and let sit
for an hour
• Then we pipetted the PB/phage mixture into a conical tube and spun for
min @ 2200 rpm
• Then using a vacuum filter we filtered the supernatant which was then
the 100 (HTL)
10. Methods (continued)
• We then did a titration of our HTL to find the titer of the HTL
• We also isolated the DNA by putting some of our phage into an oak ridge
tube, adding nuclease mix and mixing it by inversion, then we incubated
at 37 degrees C for 30 min.
• Then we let it sit for an hour at room temp.
• Then we added phage precipitant to the nuclease treated lysate and
mixed by inversion and incubated at 4 degrees C
11. Methods (continued)
• Using our phage in the oak ridge tubes we spun them in a centrifuge for
20 min at 10,000xg
• Then we poured out the supernatant (not disturbing the pellet at the
bottom) and drained the excess liquid by inverting the tube and letting it
sit for 2-3 min
• Then we added sterile water and gently re-suspended the pellet and let sit
for 5-10 min
• Then we added pre-warmed DNA clean up resin and then uncoated the
phage by pipetting the mixture up and down and swirling the tube
12. Methods (continued)
• Then using 2 columns we added some of our solution to each using a
pipette and then use a plunger to push the solution the solution through
• For each column we then pushed isopropanol through to wash the salts
and proteins off the DNA
• Then we dried the columns by centrifuging them at max speed for 5 min,
then we transferred the columns to a new tube without lid and
centrifuged if for a min at max speed
• Then we transferred it to a new tube, added pre warmed (80 degrees C) TE
to the resin in the column and let sit for a min and then centrifuged at
max speed for a min
• We combined the DNA into a single tube and stored at 4 degrees C
13. Methods (continued)
• We ran some of our DNA through a spectrometer to calculate our
micrograms per microliter
• We made gels using agarose, 1 x TAE buffer, and gel red
• We then electrophoresed 3 different gels
• The first gel was just our DNA vs. a DNA ladder
• The second gel was mixing our DNA with the enzymes BamH1, Cla1, EcoR1, Hae111,
Hind111, our DNA by itself and a DNA ladder (to see which enzyme would cut our
DNA)
• The third gel was mixing our DNA with the enzymes Pst1, Bcl1, Nco1, EcoRV with a
DNA ladder
• For the enzyme mixtures we used a mixture of 10x reaction buffer, our
DNA, 10x BSA, the enzyme and sterile water
14. MacKenzie’s Phage
• Coordinates where I got the phage: Lat 44.850554, Long -92.624617
• Location where I got the phage: A few feet from the river at the southern
end of campus
• Time and date collected- 11:30 AM September 9th, 2013
• Soil collection- moist and rich
• Soil depth- 57.2516 mm
• Air temp- 76 degrees F
• Weather conditions- Partly cloudy
• Weight of my soil – 1.47 g
15. First titer/streak plate/spot test
• I also ended up purifying the Hudson phage because my phage did not end
up yielding any plaques the first try (or when we tried to re-enrich it)
• Titer of the first set of plates (which went out from 0 to -5) – 2.42 x 104
pfu/ml
• The streak plates both had phage (of the same size, so I only continued
with one of them)
16. Purification (3 times)
• After picking the streak plate and plating it from 0 to -2, I got phage on
all of my plates (with numbers that consecutively went down as the
dilution went up), with two different sized plaques
• 1st purification (titrations 0 to -5)
• Titer of 2.42 x 104 pfu/ml
• 2nd purification (titrations 0 to -2)
• Titer of 4.55 x 104 pfu/ml
• 3rd purification (titrations 0 to -2)
• Titer of 3.6 x 105 pfu/ml
• I got two webbed plates after I picked a plaque from the third purification
17. Phage Lysate
• We made a spot plate, and we also picked a plaque from the last
purification and titrated out from 0 to -10) and I got three webbed plates
and three countable plates
• This was the spot plate
• It corresponded to my
plates that were titrated
out
• My plates ended up being
at titer of
4.05 x 107 pfu/ml
18. • This is my first countable plate (-4)
• And my webbed plate (-3)
19. HTL
• Using the webbed plates we flooded them and plated them on ten new
plates, which then yielded 10 webbed plates. We then collected a mixture
of phage and PB and filtered it (our HTL and the new 100 )
• We also did a titration of this HTL (-2 to -7) and plated it
• The titer that we got from this was 7.92 x 108 pfu/ml
20. Electrophoresis
• Using the HTL we isolated the DNA from anything else that had been in
our mixtures.
• We then mixed this isolated DNA with different enzymes so we could run
electrophoresis
• On the first test we had a group of 4 using one gel just using the DNA (2
ml of it)
• We also ran the DNA through a spectrophotometer and got the values
.376 and .218, which then calculated out to 0.188 mg/ml)
• On the second test we used the enzymes BamH1, Cla1, EcoR1, Hae111, and
Hind 111 (with 2.7 ml of my DNA)
• On the third test we used the enzymes Pst1, Bcl1, Nco1, and EcoRV (2.16 ml
of my DNA)
24. Sullivan’s Phage
• Coordinates where I got the phage: Lat 45.277801, Long -92.015927
• Location where I got the phage: My house from under a board
• Time and date collected- 1:30 PM September 8th, 2013
• Soil collection- dry and loose
• Soil depth- 55 mm
• Air temp- 74 degrees F
• Weather conditions- Cloudy, humid, occasionally misting
• Weight of my soil – 2.49 g
25. First titer/streak plate/spot test
• I also ended up purifying the Hudson phage because my phage did not end
up yielding any plaques the first try (or when we tried to re-enrich it)
• Titer of the first set of plates (which went out from 0 to -5) – 2.48 x 104
pfu/ml
26. Purification (3 times)
• After picking the streak plate and plating it from 0 to -2, I got phage on
all of my plates (with numbers that consecutively went down as the
dilution went up), with two different sized plaques
• 1st purification (titrations 0 to -5)
• Titer of 2.48 x 104 pfu/ml
• 2nd purification (titrations 0 to -4)
• Titer of 1.728 x 106 pfu/ml
• 3rd purification (titrations 0 to -4)
• Titer of 7.2 x 103 pfu/ml
27. Phage Lysate
• We made a spot plate, and we also picked a plaque from the last
purification and titrated out from 0 to -10 and I got four webbed plates
and three countable plates
• This was the spot plate
• It corresponded to my
plates that were titrated
out
• My plates ended up being
at titer of
1.202 x 1010 pfu/ml
28. • This is my first countable plate (-5)
• And my webbed plate (between -4 and -5)
• This is my -6 also
29. HTL
• Using the webbed plates we flooded them and plated them on ten new
plates, which then yielded 10 webbed plates. We then collected a mixture
of phage and PB and filtered it (our HTL and the new 100 )
• We also did a titration of this HTL (-3 to -8) and plated it
• The titer that we got from this was 3.2 x 109 pfu/ml
30. Electrophoresis
• Using the HTL we isolated the DNA from anything else that had been in
our mixtures.
• We then mixed this isolated DNA with different enzymes so we could run
electrophoresis
• On the first test we had a group of 4 using one gel just using the DNA (2
ml of it)
• We also ran the DNA through a spectrophotometer and got the values
.427 and .240, which then calculated out to 0.2135 mg/ml)
• On the second test we used the enzymes BamH1, Cla1, EcoR1, Hae111, and
Hind 111 (with 2.3 ml of my DNA)
• On the third test we used the enzymes Pst1, Bcl1, Nco1, and EcoRV (1.8 ml
of my DNA)
34. Conclusions
• Mackenzie’s plaques looked like Gordon and Sullivan’s look like Jawnski
• We may have found new phage, but not for sure because our plaques do
look like other known phages plaques