Automated system for bacterial identificationDEEKSHANT KUMAR
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Automated system for bacterial identificationDEEKSHANT KUMAR
[DOWNLOAD IT OPEN IT WITH MICROSOFT POWERPOINT THEN YOU WILL BE ABLE TO UNDERSTAND THE TOPIC COVERED.]
1. WHOLE TEXT IS RELIABLE.
2. TEXT HAS BEEN TAKEN FROM STANDARD TEXT BOOK FOR MEDICAL MICROBIOLOGY.
3. SOME PICTURE HAS BEEN TAKEN FROM JOURNAL.
Bacterial and Fungal CULTURE PRESERVATION.
SERIAL TRANSFER
PRESERVATION IN D/W
PRESERVATION UNDER OIL
LYOPHILIZATION
STORAGE OVER SILICA GEL
PRESERVATION ON PAPER
PRESERVATION ON BEADS
PRESERVATION ON SOIL
LIQUID DRYINNG.
CRYOPRESERVATION.
FROZEN AGAR PLUGS
PRESERVATION IN LIQ NITROGEN
2-STAGE FREEZING PROCESS
COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLESNCRIMS, Meerut
Principles of Sample Collection:
Aseptic precautions to minimize chances of
contamination.
Appropriate anatomic sites
Adequate volume
Adequate no. of samples
Appropriate time
Appropriate container with proper labelling
Before initiation of anti-microbials
Adequate information in request form
This presentation is made for S.Y.Bsc. Students.
The presentation includes Drinking water microbiology. The presentation includes information about coliform, indicator organisms as well as purification methods of drinking water.
Microbiology of E coli giving basic of Escherichia coli, its morphology, cultural and biochemical characteristics, Antigenic character, pathogenesis, laboratory diagnosis, prevention and control
Air is not a natural environment for microorganisms. Microorganisms present in air are liberated from various other sources. These various sources include soil, water, plant and animal surfaces and human beings.
this lecture describes the various procedures and maintenance steps that should be taken to insure that all lab equipment are working well in a controlled manner for the guarantee of accuracy of microbiological test results.
Bacterial and Fungal CULTURE PRESERVATION.
SERIAL TRANSFER
PRESERVATION IN D/W
PRESERVATION UNDER OIL
LYOPHILIZATION
STORAGE OVER SILICA GEL
PRESERVATION ON PAPER
PRESERVATION ON BEADS
PRESERVATION ON SOIL
LIQUID DRYINNG.
CRYOPRESERVATION.
FROZEN AGAR PLUGS
PRESERVATION IN LIQ NITROGEN
2-STAGE FREEZING PROCESS
COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLESNCRIMS, Meerut
Principles of Sample Collection:
Aseptic precautions to minimize chances of
contamination.
Appropriate anatomic sites
Adequate volume
Adequate no. of samples
Appropriate time
Appropriate container with proper labelling
Before initiation of anti-microbials
Adequate information in request form
This presentation is made for S.Y.Bsc. Students.
The presentation includes Drinking water microbiology. The presentation includes information about coliform, indicator organisms as well as purification methods of drinking water.
Microbiology of E coli giving basic of Escherichia coli, its morphology, cultural and biochemical characteristics, Antigenic character, pathogenesis, laboratory diagnosis, prevention and control
Air is not a natural environment for microorganisms. Microorganisms present in air are liberated from various other sources. These various sources include soil, water, plant and animal surfaces and human beings.
this lecture describes the various procedures and maintenance steps that should be taken to insure that all lab equipment are working well in a controlled manner for the guarantee of accuracy of microbiological test results.
Water quality describes the condition of the water, including chemical, physical, and biological characteristics, usually concerning its suitability for a particular purpose such as drinking.
Methods to detect potability of water samplevimala rodhe
Water is precious and it is the base for living, Several disease causing pathogens are transmitted through water. There are various methods to detect the presence of pathogens in drinking water samples.Some of the methods to detect microbiological quality of water are discussed.
Most probable number or multiple tube fermentation techniqueSamsuDeen12
multiple tube fermentation or most probable number is a microbiological technique used to check the portability of water. microbial analysis of water is determined, and distinguished between faecal and non faecal contaminated water.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
2. Bacteriology of water
• Drinking water should have the following properties
1. Biological quality
2. Chemical quality
3. Physical quality
3. Bacterial flora in water
1.Naturally occurring bacteria e.g. Micrococcus,
pseudomonas
2.Soil bacteria e.g. Bacillus subtilis, Bacillus meganterium
3.Sewage bacteria e.g. E.coli, Enterococcus faecalis,
C. perfengens, salmonella , vibrio
4. WATER BORNE PATHOGEN
BACTERIAL Vibrio cholera, E. coli, salmonella typhi, salmonella
paratyphi A,B & C, Shigella spp. , Yersinia enterocolitica
Viral Hepatitis A virus, Hepatitis E virus, Rotavirus
Protozoal E. Histolytica, Giardia lambliya, Balantidium
coli.,cryptosporium, Isosporium
Helmenthic Ascaris lumbricoides, Trichuris trichura.
5. Bacteriological Examination of Water
• INDICATOR ORGAMISM- Which satisfy two properties
1.Should be present in excess number , should not be able to
proliferate in water.
2.Should be more resistant than the pathogen.
Indicator organisms themselves are not pathogen , but their presence
in water supply indicate that there is a contamination of sewage &
the water supply needs to be disinfected.
7. Total coliforms
• Bacteria that occur in large number in faeces & sewage but also found
in environment in the absence of fecal contamination.
• Their presence in water does not necessarily signify faecal
contamination
• Member of Enterobacteriaceae
• Only E.coli is a reliable indicator as it is not found in other source.
8. Thermotolerant (faecal) Coliforms
• Term “fecal coliforms” has been used in water microbiology to denote
coliform organism which grow at 44 & 44.5 ˚ c & ferment lactose to
produce acid and gas
• Presence of thermotolerant coliforms nearly always indicates faecal
contamination.
• In thermotolerant coliforms E. coli more than 95% present.
• E. coli does not survive in water for long time & therefore is the best
indicator of recent human or animal fecal pollution of water
9. Faecal streptococci
• Faecal streptococci is evidence of faecal contamination
• Faecal streptococci tend to persist longer in the environment than
thermotolerant or total coliforms & are highly resistant to drying
• Faecal streptococci grow at 37 & 44˚c
10. Pseudomonas aeruginosa
• It can survive in the environment for the long time
• It can multiply in various aquatic habitats hence its presence in water
is not necessarily good indicator for fecal contamination
11. Bacteriological examination of water
• Collection and transport of water sample
1.Use heat sterile bottles containing sufficient volume of sodium
thiosulphate to neutralize the bactericidal effect of any chlorine
2.Each bottle of 100ml capacity should contain 0.1 ml of fresh 1.8%
aqueous solution of sodium thiosulphate.
3.When collecting the sample from taps, allow water to run to water
for 2-3 min before running it to bottle
4.Water from steam or lakes- the bottle should be opened only after
immersed at a depth of 30 cm with its mouth facing the current
5.Transport- bottle should be properly labeled and sent to lab as
quickly as possible at least within 6 hours
12. Presumptive coliform count (multiple tube method)
• Standard test employed for bacteriological analysis of water.
• Only procedure that can be used if water sample are very turbid or if
semi solids such as sediments are to be analyzed.
• In multiple tube method, the presumptive coliform count is
determined, which is expressed as the most probable number (MPN)
of coliform organism in 100 ml of water.
Medium – MacConkey purple broth ( double strength and single
strength) in bottles or tubes.
Inverted Durham’s tube is used to detect gas production
Bromocresol purple is used as indicator
13. Requirement
• One bottle with loose fitting caps calibrated at 50 and 100 ml for 50
ml of sample + 50 ml of culture media
• 10 test tubes for 10 ml of sample + 10 ml of culture medium
• Test tube rack
• Measuring cylinder of different capacity
• Durham's tubes
• Double and single strength MacConkey broth
14. MacConkey broth, double strength
Peptone 40g
Sodium taurocholate 10g
Lactose 20g
Sodium chloride 10g
Bromocresol purple , 1% (w/v) in ethenol 2ml
Distilled water 1liter
Dilute the double strength medium with an equal volume of D.W. to make
single strength
15. procedure
• Place 50 ml and 10 ml volume of double strength concentration and 5
ml volume of single strength into suitable size of bottle or tube
containing an inverted Durham's tube
• Aseptically pipette one 50 ml volume and five 10 ml volume of the
water in the vessel containing corresponding volume of double
strength medium.
• Pipette five 1 ml volume into vessel containing single strength
medium.
• Incubate the media aerobically at 37˚C
16. Cont…..
• After 24 h and 48h of incubation inspect the media and note the
culture of each volume of water that show the production of acid
(color change) and gas (a bubble at the top of Durham's tube)
• In respect of the combination of positive and negative result read off
the MOST PROBABLE NUMBER (MPN) of presumptive coliform
bacteria present in 100 ml of the sampled water.
17.
18.
19. Eijkman test
• It is done to confirm that the coliforms bacilli detected in the
presumptive test are fecal E.coli. This is done by
Sub culturing the positive tubes on lactose containing medium such
as brilliant green bile broth for detection of lactose fermentation of
acid and gas at 44˚c
Demonstrating positive indole test at 44˚c.
20. Other methods of water analysis
Membrane filtration method
• Principle –
• this method is based on the filtration of known volume of water
through a cellulose membrane with pore diameter of 0.45 -0.2µm.
• the bacteria are retain on the surface of membrane filter , such
membrane is transferred on to a petri dish containing selective
differential culture medium at an appropriate temperature ( 37 and
44˚c) and incubate; charrecterstics colonies of coliforms developes
which can be count didectly.
21. Air surveillance
• Air is important vehicle of transmission of many pathogenic
organisms
• The examination of air to detect the number of bacteria carrying
particle is important particularl in critical areas such as OTs. ,bone
marrow transplant units etc.
• Routine air sampling is not recommended because-
• There is no standard guideline mentioning for permissible level of
microbial contamination of environmental surface or air.
• HAI rates are not related with levels of general microbial
contamination of air or environmental surface .
22. CDC recommends targeted air surveillance , which should be carried out for-
• Investigation of an outbreak.
• For research purpose.
• After reconstruction or newly constructed buildings.
• After fumigation or fogging ( to monitor the quality)
23. EVALUATION OF QUALITY OF AIR IN OT
Microbiological parameters
• Can be performed routinely by two broad methods
1. Microbiological sampling method
2. Particle count method
24. Microbiological sampling method
• Can be done in two ways
1.Method that can measure bacteria carrying particle settle down by
gravity from air e.g. settle plate method
2.Method that can count number of bacteria carrying particle in a given
volume of air e.g. The slit sampler and air centrifugation method.
25. Particle count method
• The airborne particle concentrations in OT is measure by means of
laser light scattering instrument ( particle counter)
26. Settle plate method
• Petri dishes containing an agar medium of known surface area are left
open for 30 min. to 1 hour. Then , the plates are incubated at 37 c for
24 hours
• Colony count : large bacteria carrying dust particle settle onto the
medium. The number of colony formed onto the plate indicates the
number of settle particle containing bacteria.
• Blood agar is the preferred medium for an overall count of
pathogenic, saprophytic and commensal bacteria.
• Malt extract agar may be used for molds
27.
28. Slit sampler method
• Most efficient and convenient method for counting the number of
bacteria carrying particle suspended in a unit of volume of air
• Procedure – A special equipment called “ slit sampler” has three part
1.An area to hold a petri dish.
2.Suction pump.
3.Outer surface has a slit of 0.33 mm width and 27.5 mm length and
3mm depth.
29.
30.
31. Milk surveillance
• Milk can be occasionally contain bacteria which are derived from
three sources
1.From animals
2.From hand of the milk handler
3.From the environment
32. Methods used for disinfecting/Sterilizing milk
1.Thermized milk- it is raw milk that had been heated for 15 sec at 57-
68˚c
2.Pasteurization- milk is heated to high temperature for short time (
72˚c for 15 sec.)
3.Ultra heat treated milk- milk is expose to very high temperature of
135˚c for 1 sec so that all microorganisms with their spores are
destroyed.
4.Sterilized milk- the milk is heated at 100˚ c for long period .
33. Bacteriological examination of milk
• Chemical tests-
Methylene blue reduction test
Phosphate test-
it is based on the principle that if pasteurization is effective , it should
inactive the enzyme alkaline phosphate which is normally present.
Turbidity test
34. Methylene blue test
• Rapid ,inexpensive way of indicating poor quality of milk that had
been unrefrigerated .
• The basis of test is that the length of time for bacterial
dehydrogenases to reduce methylene blue dye and decolorize it is an
indicator of the number of viable bacteria.