Pesticide residues refer to any pesticide substance or its metabolites that remain in food, water, or animal feed after application. This document outlines methods for analyzing pesticide residues, including sampling procedures, sample preparation techniques like homogenization, common extraction methods like solid phase extraction, cleanup steps to remove interferences, and quantification methods like gas chromatography and liquid chromatography. It emphasizes the importance of analyzing pesticide residues to ensure food safety and compliance with legal limits.
Pesticide residue analysis by simran.pptxSimran Bhatia
INTRODUCTION
The importance of food quality has become a serious issue due to the widespread use of pesticides. Though, the farmers have a conventional understanding of agriculture; they lack in the technical understanding of pesticides, their uses and safety aspects which makes them vulnerable (FAO, 2011).
Pesticide Residue: - The amount of insecticide left over after a lapse of time.” Disappearance of pesticide residue takes place by two ways i.e. Dissipation (In which the disappearance of the residue is fast) and Persistence (ability of pesticide to remain present for a long time).
Steps in Pesticide residue analysis:-
Sampling (collection, transport & storage)
Sample Preparation (Chopping/grinding/blending)
Extraction : Removal of pesticide residue from components that are present naturally
Clean-up : Removal of constituents that interfere with analysis of the pesticide residue of interest (Partitioning and purification )
Identification (detection), Quantification (determination) & Confirmation
4. EXTRACTION__________________________________________________________
Extraction is the process by which toxicant is transferred from the treated bulky biological material in to a solvent.
Principle : LIKE DISSOLVES LIKE and OPPOSITES DO NOT ATTRACT
QuEChERS_________________________________________________________________
Quick, Easy, Cheap, Effective, Rugged, and Safe.
◦ Developed in 2001 by American scientist Michelangelo
◦ Based on Acetonitrile extraction
◦ cleanup using dispersive-solid phase extraction (d-SPE) using MgSO4 and further purification with PSA and silica sorbent C18
◦ This method became popular because of its microscale extraction procedure which is simpler, consumes less time and organic solvent than all the previous methods.
Flow chart for QuEChERS sample preparation technique for pesticide residue analysis in FRUITS AND VEGETABLES
Status of Maximum Residue Limits in India:
The Central Insecticides Board and Registration Committee (CIBRC) registers pesticides in India and recommends them for various crops.
A total of 234 pesticides have been registered by CIBRC (including Endosulfan* the use of which was banned in India in May 2011).
Food Safety and Standard Authority of India (FSSAI) is responsible for setting MRLs for the pesticides that have been registered by CIBRC.
The MRLs for all registered pesticides should be set for all the crops they have been registered.
Pesticide residue analysis by simran.pptxSimran Bhatia
INTRODUCTION
The importance of food quality has become a serious issue due to the widespread use of pesticides. Though, the farmers have a conventional understanding of agriculture; they lack in the technical understanding of pesticides, their uses and safety aspects which makes them vulnerable (FAO, 2011).
Pesticide Residue: - The amount of insecticide left over after a lapse of time.” Disappearance of pesticide residue takes place by two ways i.e. Dissipation (In which the disappearance of the residue is fast) and Persistence (ability of pesticide to remain present for a long time).
Steps in Pesticide residue analysis:-
Sampling (collection, transport & storage)
Sample Preparation (Chopping/grinding/blending)
Extraction : Removal of pesticide residue from components that are present naturally
Clean-up : Removal of constituents that interfere with analysis of the pesticide residue of interest (Partitioning and purification )
Identification (detection), Quantification (determination) & Confirmation
4. EXTRACTION__________________________________________________________
Extraction is the process by which toxicant is transferred from the treated bulky biological material in to a solvent.
Principle : LIKE DISSOLVES LIKE and OPPOSITES DO NOT ATTRACT
QuEChERS_________________________________________________________________
Quick, Easy, Cheap, Effective, Rugged, and Safe.
◦ Developed in 2001 by American scientist Michelangelo
◦ Based on Acetonitrile extraction
◦ cleanup using dispersive-solid phase extraction (d-SPE) using MgSO4 and further purification with PSA and silica sorbent C18
◦ This method became popular because of its microscale extraction procedure which is simpler, consumes less time and organic solvent than all the previous methods.
Flow chart for QuEChERS sample preparation technique for pesticide residue analysis in FRUITS AND VEGETABLES
Status of Maximum Residue Limits in India:
The Central Insecticides Board and Registration Committee (CIBRC) registers pesticides in India and recommends them for various crops.
A total of 234 pesticides have been registered by CIBRC (including Endosulfan* the use of which was banned in India in May 2011).
Food Safety and Standard Authority of India (FSSAI) is responsible for setting MRLs for the pesticides that have been registered by CIBRC.
The MRLs for all registered pesticides should be set for all the crops they have been registered.
Organic carbon in organic matter is oxidized by known but excess of chromic acid. The excess chromic acid not reduced by organic matter is determined by back titration with standard ferrous sulphate solution, using diphenylamine or ferroin indicator. The organic carbon content in soil is calculated from the chromic acid utilized (reduced) by it.
Pesticides have been linked to a number of health problems, including neurologic and endocrine (hormone) system disorders, birth defects, cancer, and other diseases.
Determination of soil available nitrogen by Alkaline
permanganate method (Subbiah and Asija, 1956).
Nitrogen is necessary for all forms of life. It is most important
essential plant nutrient for crop production as it is constituted the building blocks of almost all the plant structures.
This lecture is based on previously read lecture "Plant Mineral Analysis", 2012. Some new points were added, especially in LOD/LOQ section. The internal standard calculation was explained. The lecture was presented in the frame of International Course "Crop Production under Saline Stress As A Result Of Climatic Changes", The Faculty of Agriculture, The Hebrew University of Jerusalem.
GCMS & LCMS
htps://youtube.com/vishalshelke99
https://instagram.com/vishal_stagram
Sub :- Advanced Analytical Techniques
M.Pharmacy Sem1
Savitribai Phule Pune University
Contents :-
GC-MS
Introduction
Principle
Instrumentation
Application
LC-MS
Introduction
Principle
Instrumentation
Application
Introduction to Gas chromatography-Mass spectroscopy
Gas chromatography-Mass spectroscopy is one of the so-called hyphenated analytical techniques. It is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals
GC-MS is an instrumental technique, comprising a gas chromatograph coupled to a mass spectrometer by which complex mixtures of chemicals may be separated, identified & quantified. In order to a compound to be analysed by GC-MS it must be sufficiently volatile & thermally stable.
Principle :-
The Sample solution is injected into the GC inlet where it is vapourized & swept onto a chromatographic column by the carrier gas ( usually helium). The sample flows through the column & compounds comprising the mixture of interest are separated by virtue of their relative interaction with the coating of the column (stationery phase) & the carrier gas (mobile phase). The later part of the column passes through a heated transfer line & ends at the entrance to ion source where compounds eluting from the column are converted to ions
Soil parameters, analysis protocols, interpretation, and fertilizer recommend...ExternalEvents
The second lab managers’ meeting of the South-East Asia Laboratory NETwork (SEALNET) took place on 19 - 23 November 2018 in ICAR-IISS (Indian Institute of Soil Science), Bhopal, India.
Drs. Sanjay Srivastava and Pradip Dey, ICAR-IISS, Bhopal, India (1st Day)
Organic carbon in organic matter is oxidized by known but excess of chromic acid. The excess chromic acid not reduced by organic matter is determined by back titration with standard ferrous sulphate solution, using diphenylamine or ferroin indicator. The organic carbon content in soil is calculated from the chromic acid utilized (reduced) by it.
Pesticides have been linked to a number of health problems, including neurologic and endocrine (hormone) system disorders, birth defects, cancer, and other diseases.
Determination of soil available nitrogen by Alkaline
permanganate method (Subbiah and Asija, 1956).
Nitrogen is necessary for all forms of life. It is most important
essential plant nutrient for crop production as it is constituted the building blocks of almost all the plant structures.
This lecture is based on previously read lecture "Plant Mineral Analysis", 2012. Some new points were added, especially in LOD/LOQ section. The internal standard calculation was explained. The lecture was presented in the frame of International Course "Crop Production under Saline Stress As A Result Of Climatic Changes", The Faculty of Agriculture, The Hebrew University of Jerusalem.
GCMS & LCMS
htps://youtube.com/vishalshelke99
https://instagram.com/vishal_stagram
Sub :- Advanced Analytical Techniques
M.Pharmacy Sem1
Savitribai Phule Pune University
Contents :-
GC-MS
Introduction
Principle
Instrumentation
Application
LC-MS
Introduction
Principle
Instrumentation
Application
Introduction to Gas chromatography-Mass spectroscopy
Gas chromatography-Mass spectroscopy is one of the so-called hyphenated analytical techniques. It is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals
GC-MS is an instrumental technique, comprising a gas chromatograph coupled to a mass spectrometer by which complex mixtures of chemicals may be separated, identified & quantified. In order to a compound to be analysed by GC-MS it must be sufficiently volatile & thermally stable.
Principle :-
The Sample solution is injected into the GC inlet where it is vapourized & swept onto a chromatographic column by the carrier gas ( usually helium). The sample flows through the column & compounds comprising the mixture of interest are separated by virtue of their relative interaction with the coating of the column (stationery phase) & the carrier gas (mobile phase). The later part of the column passes through a heated transfer line & ends at the entrance to ion source where compounds eluting from the column are converted to ions
Soil parameters, analysis protocols, interpretation, and fertilizer recommend...ExternalEvents
The second lab managers’ meeting of the South-East Asia Laboratory NETwork (SEALNET) took place on 19 - 23 November 2018 in ICAR-IISS (Indian Institute of Soil Science), Bhopal, India.
Drs. Sanjay Srivastava and Pradip Dey, ICAR-IISS, Bhopal, India (1st Day)
Cross Flow or Tangential Flow Membrane Filtration (TFF) to Enable High Solids...njcnews777
Cross Flow or Tangential Flow Filtration (TFF) Membrane Plants are used in Desalination, Brackish Groundwater Treatment, High Chloride Surface Water Treatment, Waste Water Treatment Plant Effluent Reuse, Biopharmaceutical, Food & Protein Applications for removal of undesired constituents and harvesting of desireable products. Cross flow membrane filtration technology has been used widely in industry globally. Filtration membranes can be polymeric or ceramic, depending upon the application. The principles of cross-flow filtration are used in reverse osmosis, nanofiltration, ultrafiltration and microfiltration. When purifying water, it can be very cost effective in comparison to the traditional evaporation methods. Techniques to improve performance of cross flow filtration include:
Backwashing: In backwashing, the transmembrane pressure is periodically inverted by the use of a secondary pump, so that permeate flows back into the feed, lifting the fouling layer.
Clean-in-place: Clean-in-place systems are typically used to remove fouling from membranes after extensive use. The CIP process may use detergents, reactive agents such as sodium hypochlorite and acids and alkalis such as citric acid and sodium hydroxide.
Concentration: The volume of the fluid is reduced by allowing permeate flow to occur. Solvent, solutes, and particles smaller than the membrane pore size pass through the membrane, while particles larger than the pore size are retained, and thereby concentrated. In bioprocessing applications, concentration may be followed by diafiltration.
Diafiltration: In order to effectively remove permeate components from the slurry, fresh solvent may be added to the feed to replace the permeate volume, at the same rate as the permeate flow rate, such that the volume in the system remains constant. This is analogous to the washing of filter cake to remove soluble components. Dilution and re-concentration is sometimes also referred to as "diafiltration."
irrational usage of pesticide leads to development of resistance, resurgence and toxic residue problems in our food. ultimately imbalance of environment . so that detection of pesticide residue in all materials of earth especially in our food, milk, meat, water, soil aquatic ecosystem and agriculture land. for the analysis of resiude set of procedure, methods, instruments, skills and laboratory must required. In this seminar would like to enlighten the best, suitable and feasible methods are discussed.
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In this assignment file i will demonstrate the process involved in Thin layer chromatography for Chlorinated Pesticide
determination. if u like this work feel free to share this file. thank you.
Ethnobotany and Ethnopharmacology:
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Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
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The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
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Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
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This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
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Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptx
Pesticide Residue Analysis
1. PESTICIDE RESIDUE ANALYSIS
Presented By: Manish Joshi
Department of Entomology, SDAU, SK Nagar (Gujarat)
Submitted To: Dr. P. S. Patel (Assoc. Prof.)
Department of Entomology, SDAU, SK Nagar (Gujarat)
2. Pesticide
Pest- “Pest is any harmful, destructive, or
troublesome animal, plant or microorganism.”
Pesticide- “Pesticide is any substance or mixture
of substances intended for preventing, destroying,
repelling, or mitigating any pest.”
• US Environmental Protection Agency (EPA)
3. WHY DO WE NEED PESTICIDES ?
1. Protect Crop losses/ yield reduction
2. Protection during Storage
3. Vector Disease Control
India produces 16% of the world’s food grain but using less than 2% pesticides
used world-wide. Hence there is a good potential of growth.
4. Pesticide : Side effects on human body
Agrochemicals and Security: Homeland Security and Pesticides Pest-09
Acute exposure
Chronic exposure
Inhalation
Absorption through skin
Through food or water
How pesticides get in
Exposure levels
Central nervous system
Skin irritation
Eye irritation
Health effects
(depends on specific pesticide)
Hormone imbalance
Cancer
Liver damage
Reproductive effects
9. Pesticide Residues means any specified
substances in food, agricultural commodities,
or animal feed resulting from the use of a
pesticide.
The term includes any derivatives of a
pesticide, such as conversion products,
metabolites, reaction products and
impurities considered to be of toxicological
significance.
9
What is Pesticide Residue?
10. Terminology
Residue : When pesticides is applied on the commodity, its
parent compound or metabolism having pharmacological
property and/or toxicological nature, together termed as residue.
Maximum residual limit (MRL) : The limit beyond which
pesticide levels are not legally permitted.
Acceptable daily intake (ADI) : It is the daily dose of chemical
which during an entire life time, appears to be without
appreciable risk on the basis of all facts known at that time.
Waiting period (TMRL) : The determination of the minimum
interval required between application of pesticide and harvesting
of crop.
RL50 or T½ (Half-life value) : It is the time in days required to
reduce the pesticide residues to half of its initial deposits.
10
11. Persistence and Degradation
Persistence – The chemical that remain active for a
long period of time after application.
Degradation – The breakdown of a substance into
simpler molecular or atomic components through
chemical reaction(s) either in a plant or animal
(metabolic degradation) or in the environment
(environmental degradation)
11
12. Deposit: The amount of initially laid down
pesticide on the surface is called deposit.
Dissipation and persistence: In nature,
disappearance of residues takes place in two
steps.
The first step is the initial phase in which the
disappearance of the residue is fast. This phase is
called "Dissipation".
The second phase, in which there is a slow
decrease in the amount of residue, is known as
"Persistence".
12
13. Workflow for analysis of pesticide
residues
sampling
Sample
preparation
Sample
Analysis
Data
evaluation
And Reporting
15. 15
SAMPLING PROCEDURES
Sample collection is the process of taking
portion(s) of a food or other substance, for
laboratory analysis, so that resulting analytical
data and conclusions can be applied to the
original food or substance sampled.
17. 17
Water
• Underground water
• Do not take a sample from tank
• Purge for 10 minutes
• Collect water in washed glass bottle
• Collect 2.5 L water to represent one sample
• Transport sample to the laboratory at 40C
18. 18
SAMPLE SIZE
• Samples of 2.5 to 5 liter / sampling site
• These are reduced with through mixing and
by filling samples in to 1 to 2 bottles
19. 19
SAMPLING PROTOCOL
• Label it immediately
• Also record :
Geography depth & diameter
Exact location
Date and time of sampling
Water volume
Water and air temperature
Weather parameter
Sampling personal
21. 21
SAMPLING
• Sample taken with auger
• In grass land – 10 cm
• In forest land – 20 cm
• In orchard – 0 to 25 & 25 to 50 cm
• Sampling site spread evenly
• Sample should not taken from border strip or
places where pesticides have been transferred
from container to tank or where spray have
been mixed
• In large field, the sampling site should be 5 m
away from field periphery or above site
22. 22
SAMPLE SIZE
• In small field : 3 x 3 grid
• In medium size field : 4 x 4 grid
• In large size field : 5 x 5 grid
• Same in untreated plots
23. 23
Sample size
Type
Unit
weight (g)
Minimum amount
to be collected
Small or light units
e.g. Berries, peas etc.
25 g or less 1 kg
Medium sized units
e.g. Apple, orange, carrot, potato
25 - 250 g
1 kg
(At least 10 units)
Large sized units
e.g. Cabbage, cauliflower, melons
>250 g
2 kg
(At least 5 units)
Dairy products
e.g. Butter, Milk cream, Cheese
0.5 kg
Poultry products e.g. egg 0.5 kg
Animal tissue e.g. Meat, Chicken, Fish and
their products
0.5 kg
Oil and fats e.g. Cotton seed 0.25 kg
Spices e.g. Cumin, Fennel 0.5 kg
Cereals e.g. Wheat, Rice 1.0 kg
24. Sample preparation
• Reduce the sample – sub sampling
• Store reserve portion of the sample
• Homogenize the sub sample by :-
Chopping
Grinding
Blending
• For this, knives, grinder, warning blender are used.
24
25. Extraction process
• There are several extraction approaches
employed such as:
• Pressurized liquid extraction(PLE)
• Supercritical fluid extraction (SFE)
• Aqueous extraction
• Microwave-assisted extraction(MAE)
• Solid-phase extraction (SPE)
• Solid-phase microextraction (SPME)
• Matrix solid-phase dispersion (MSPD)
This is a
fundamental
process in
pesticide residue
analysis because
no in-situ
technique has
yet been
invented.
Extraction is the process by which toxicant is transferred from the
treated bulky biological material in to a solvent.
26. Pressurized liquid extraction(PLE)
PLE Is an accelerated solvent extraction.
Uses liquid for extraction.
Samples are loaded into vessels, which require a dispersant/drying agent to
aid solvent flow.
Extraction occur at elevated temperature I.e room temp to 40-200 and
pressure 1000-3000 psi to improve recoveries.
Static extraction mode is used to equilibrate the sample to extraction
conditions and dissolve the analytes in liquid.
Vessel is flushed with solvent rapidly, rather than a controlled dynamic flow in
Superficial Fluid Extraction using a restrictor thus making PLE faster than SFE.
Any liquid can be used provided it can be pumped through sample and its
pH is between 2-12.
27. Supercritical fluid extraction (SFE)
SFE uses supercritical fluid as an extraction solvent.
CO2 is common solvent used in SFE, due to its safe nature,
uncreativeness, ready availability and inexpensiveness.
The rest of the process is similar to that of Pressurized
Liquid Extraction.
28. Solid-phase Extraction
Solid-phase extraction is a separation process by which compounds that
are dissolved or suspended in a liquid mixture are separated from other
compounds in the mixture according to their physical and chemical
properties.
SPE consists of four steps: column preparation (prewash), sample loading
(retention or sorption), column post wash, and sample desorption (elution
or desorption).
compounds of interest are retained on the sorbent while interferences are
washed away.
Analytes are recovered via an elution solvent.
29. Clean-up
Clean-up is done to obtain uncontaminated products for analysis.
The clean-up process is vital especially in analysis of fatty foods.
Liquid-liquid and liquid-solid partitioning in which separation is based on polarity is the
primary mean of clean-up in residue analysis.
Separations based on molecular weight, gel-permeation or size exclusion chromatography is
common clean-up technique.
Other techniques used include precipitation, centrifugation, ultrafiltration, dialysis and
immunochemical.
Isolation of toxicant from interfering substances or solvents.
31. Spectrophotometric Method
• Three kinds of spectrophotometric method used in pesticide
residue analysis.
• Ultraviolet, visible and infra-red methods.
• Measure the conc. of pesticide in solution by measuring the
amount of the light that is absorbed by the solution.
• Range 180nm to 400nm
32. Chromatography
• The chromatography consist in the unique distribution of the
components of a mixture between stationary phase and
mobile phase due to different affinity of a single compounds
between forward both phase.
• It involve passing a mixture dissolved in a “mobile phase”
through a stationary phase, which separates the analytes to
be measured from other molecules in the mixture based on
differential distribution coefficient and different net rate of
migration between the mobile and stationary phase.
Chromo means “colour” & Graphy means “ To write”
33. Various techniques of chromatography
• Paper Chromatography
• Thin layer
Chromatography
Planar
Chromatography
• Gas Chromatography
• Liquid Chromatography
Column
Chromatography
34. Planar Chromatography
• Planar chromatography is a separation technique
in which the stationary phase in or on a plane.
• The plane can be paper, serving as such or
impregnated by a substance as the stationary bed
(Paper Chromatography)
OR
• A layer of solid particles spread on a support such
asa glass plate ( Thin layer chromatography)
35. Column Chromatography
• Stationary phase is placed into glass or metal column.
• The mixture of analytes is then applied and the mobile
phase, commonly referred to as the Eluent passed
through column either gravity feed or by use of
pumping system or applied gas pressure.
• The stationary phase either coated on to discrete small
particles (Matrix) and packed in to column.
• As the Eluent flow through the column analyte
separate on the basis of their distribution coefficient
and emerge individually in the eluent as it leaves the
column.
36. Gas Chromatography(GC)
• Volatility and thermal stability.
• Carrier gas- Helium, Argon and Nitrogen: Which are
chemically inert.
• The stationary phase is adhered to the inside of a small-
diameter glass tube ( a capillary column) or a solid matrix
inside a larger metal tube ( a packed column).
• 80% of pesticide can be analysed.
• Organochlorines, Organophosphates, Carbamates
37. Pesticide detected using GC
Organochlorines e.g. Dieldrine, DDT, Lindane
Organophosphorus e.g. Diazinon, dichlorovos,
malathion
Pyrethroid e.g. cypermethrin, deltamethrin
Aromatic fungicides e.g. Chlorothalonil, quintozene
Carbamates, imidazole, benzoylureas, sulfonylureas
38. Liquid Chromatography (LC)
• It is a separation technique in which the mobile phase is a
liquid. Present day liquid chromatography that generally
utilizes very small packing particles and a relatively high
pressure is referred to as high performance liquid
chromatography (HPLC).
• In the HPLC techniques, the sample is forced through a
column that is packed with irregularity or spherically shaped
particles or a porous monolithic layer ( Stationary phase) by a
liquid (Mobile phase) at high pressure.
39. Carbamates e.g. Carbendazim
Conazoles e.g. Fenamidone
Sulfonylureas e.g. triasulfuron
Triazines e.g. Simazine
Phenylureas e.g. linuron
Strobilurins e.g. Azoxystrobin
Neonicotinoids e.g. Acetamiprid
All the above plus their metabolites can be analysed by liquid
chromatography
Pesticide detected using LC
40. QuEChERS- Multiresidue-Method
ADVANTAGES
Rapid (8 Samples in Less Than 30 min)
Simple (No Laborious Steps, Minimal Sources of
Errors)
Cheap
Low Solvent Consumption (10 ml Acetonitrile)
Practically no Glassware Needed
Wide Pesticide Range (Polar, pH-Dependent
Compounds)
Extract in Acetonitrile (GC- and LC-Amenable)
41. Avoid un-judicial use of pesticides
Use only recommended dose of pesticides
Use pesticides only need base
Follow safe harvesting period i.e. waiting period
Avoid to use of pesticide near to ripening stage of crop
Follow washing and cooking before use of vegetables
Use botanicals / microbial insecticides for the control
of various crop pests
Purchase pesticides only from authorized dealers
Banned pesticides should not be used for pest control
Get detailed information from the authorized people
before mixing of various pesticides.
Follow IPM & GAP
Steps to overcome
pesticide residue problems
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