The purpose of this course is to develop participant fish identification skills and knowledge of regional freshwater fish species. Participants will learn an overall system for identifying fish. Characteristics of major taxonomic groups within each
family will provide the basis to approach species-level identification. Although emphasis will be placed on the families Cyprinidae, Percidae, Centrarchidae, Catostomidae, and Ictaluridae, specimens from 25 North American freshwater fish
families will be available for study. This course is "hands on" and lab-intensive. Field exercises will provide fresh specimens for identification.
Key Learning Objectives
- Learn how the use of automated software can make SRM development faster and more highly optimized.
- Learn how the use of a compound data store can further simplify method creation.
- Learn how the use of retention time-based SRM acquisition can increase MS/MS sensitivity and make method maintenance easier.
Event Overview:
In recent years, Gas Chromatography-triple quadrupole mass spectrometry has increased in popularity due to its ability to offer lower detection limits in complex matrices, simplified sample prep requirements, and faster analysis times. Of course, new instrument technology presents the need for the acquiring of new skills to harness the advantages offered by its adoption into current workflows.
In this webinar, a strategy for addressing both of these challenges is discussed in the context of new software designed to automate common method development and method maintenance tasks. Also, in addition to making the triple quadrupole easier to use, this strategy can increase sensitivity of the analysis, which will be demonstrated using a complex SRM pesticide method as an example.
For more information: www.thermoscientific.com/tsq8000
Waters analytical technologies enable laboratories to generate more information, complete analyses more rapidly and reduce overall costs throughout key steps in the agrochemical development workflow - including Synthetic Chemistry, Purification, Formulation, Trace Detection, Metabolite ID.
This presentation compares wo methods for the detection of low-level pesticide residues in fruit juice. One involves the use of QuEChERS sample preparation and the other a 'dliute and shoot' approach. Sample preparation is utilised to remove the matrix effects associated with mass spectrometry (MS), using a 'dilute and shoot' approach requires the use of highly sensitive MS detection. It can be seen from the results shown that the 'dilute and shoot' approach can be used in many cases.
LC-MS/MS Solutions for Toxicology and Clinical ResearchSCIEX
La spectrométrie de masse en tandem est devenue un outil essentiel pour les applications cliniques et la recherche biomédicale impliquant l’analyse de biomarqueurs. Contrairement aux méthodes classiques de dosage immunologique, l’analyse par chromatographie liquide couplée à la spectrométrie de masse (LC-MS/MS) permet une analyse très sélective et spécifique de composés multiples en un seul passage, conduisant à une plus grande confiance dans les résultats et permettant de nouvelles découvertes. La LC-MS/MS est idéalement adaptée pour identifier les hormones stéroïdes, la vitamine D et ses métabolites, des peptides ou des protéines et d'autres composés dans des matrices complexes tels que le sang, l'urine, la salive et les lysats céllulaires. Aujourd'hui, la LC-MS/MS est également la méthode préférée de l'analyse médico-légale surpassant les techniques d'analyse traditionnelles - à la fois à des fins de dépistage et de confirmation.
Cette présentation vous présentera comment SCIEX pourrait contribuer à améliorer le monde dans lequel nous vivons en permettant aux scientifiques et aux analystes de laboratoire de trouver des réponses aux défis analytiques complexes auxquels ils sont confrontés.
Key Learning Objectives
- Learn how the use of automated software can make SRM development faster and more highly optimized.
- Learn how the use of a compound data store can further simplify method creation.
- Learn how the use of retention time-based SRM acquisition can increase MS/MS sensitivity and make method maintenance easier.
Event Overview:
In recent years, Gas Chromatography-triple quadrupole mass spectrometry has increased in popularity due to its ability to offer lower detection limits in complex matrices, simplified sample prep requirements, and faster analysis times. Of course, new instrument technology presents the need for the acquiring of new skills to harness the advantages offered by its adoption into current workflows.
In this webinar, a strategy for addressing both of these challenges is discussed in the context of new software designed to automate common method development and method maintenance tasks. Also, in addition to making the triple quadrupole easier to use, this strategy can increase sensitivity of the analysis, which will be demonstrated using a complex SRM pesticide method as an example.
For more information: www.thermoscientific.com/tsq8000
Waters analytical technologies enable laboratories to generate more information, complete analyses more rapidly and reduce overall costs throughout key steps in the agrochemical development workflow - including Synthetic Chemistry, Purification, Formulation, Trace Detection, Metabolite ID.
This presentation compares wo methods for the detection of low-level pesticide residues in fruit juice. One involves the use of QuEChERS sample preparation and the other a 'dliute and shoot' approach. Sample preparation is utilised to remove the matrix effects associated with mass spectrometry (MS), using a 'dilute and shoot' approach requires the use of highly sensitive MS detection. It can be seen from the results shown that the 'dilute and shoot' approach can be used in many cases.
LC-MS/MS Solutions for Toxicology and Clinical ResearchSCIEX
La spectrométrie de masse en tandem est devenue un outil essentiel pour les applications cliniques et la recherche biomédicale impliquant l’analyse de biomarqueurs. Contrairement aux méthodes classiques de dosage immunologique, l’analyse par chromatographie liquide couplée à la spectrométrie de masse (LC-MS/MS) permet une analyse très sélective et spécifique de composés multiples en un seul passage, conduisant à une plus grande confiance dans les résultats et permettant de nouvelles découvertes. La LC-MS/MS est idéalement adaptée pour identifier les hormones stéroïdes, la vitamine D et ses métabolites, des peptides ou des protéines et d'autres composés dans des matrices complexes tels que le sang, l'urine, la salive et les lysats céllulaires. Aujourd'hui, la LC-MS/MS est également la méthode préférée de l'analyse médico-légale surpassant les techniques d'analyse traditionnelles - à la fois à des fins de dépistage et de confirmation.
Cette présentation vous présentera comment SCIEX pourrait contribuer à améliorer le monde dans lequel nous vivons en permettant aux scientifiques et aux analystes de laboratoire de trouver des réponses aux défis analytiques complexes auxquels ils sont confrontés.
This webinar will provide pesticides residue analysts with valuable information on the development and optimization of chromatographic separations and mass spectrometry methods for the analysis of pesticide residues in food. The expert speakers will share their knowledge in understanding the critical aspects of the method, assisting analysts in optimizing their methods for the most challenging analyses.
mass spectrometry for pesticides residue analysis- L2sherif Taha
This is the second lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (2) include: Electron ionization and Chemical ionization
Carbon 14 and archeological ages, Christian and Intelligent Design discussion of source, measurement, results, interpretation, and errors in Carbon-14 dating.
MASS SPECTROMETRY IN THE FIELD OF FOOD INDUSTRYErin Davis
This is a powerpoint presentation solely to give a brief idea about the role of Mass Spectrometry (MS) which is one of the powerful analytical technique.This presentation describes the role of Mass Spectrometry in the field of food industry.These slides deals with the basic principle,working,components,detailed analysis etc.
Mike Riddle has put together a brilliant slideshow examining the truths and misnomers of dating methods currently used by scientists eager to propogate their evolutionary agenda. I hope you enjoy Mr. Riddle's slidshow as much as I have.
mass spectrometry for pesticides residue analysis- L3sherif Taha
This is the third lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (3) include: Electrospray ionization and Atmospheric pressure chemical ionization
mass spectrometry for pesticides residue analysis- L1sherif Taha
This is the first lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (1) include Pesticides classification, introduction to mass spectrometry, vacuum system for Agilent GC MS/ MS and AB SCIEX LC MS/ MS
The purpose of this manual is to help trainers provide training-cum-demonstration support on fish pond management techniques. It can be used to teach farmers the concepts, skills and practices of good pond management that are vital for producing economically feasible yields and solving pond management problems.
In addition, this manual is useful as a quick-reference and self-help guide for fishery facilitators, fish farmers and extension workers. It is designed to arouse the interest of fish farmers and the general public, thereby stimulating greater investment in fish farming. This simple training and demonstration manual has the potential to greatly improve the practices of fish farmers and help promote rapid rural aquaculture development.
This webinar will provide pesticides residue analysts with valuable information on the development and optimization of chromatographic separations and mass spectrometry methods for the analysis of pesticide residues in food. The expert speakers will share their knowledge in understanding the critical aspects of the method, assisting analysts in optimizing their methods for the most challenging analyses.
mass spectrometry for pesticides residue analysis- L2sherif Taha
This is the second lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (2) include: Electron ionization and Chemical ionization
Carbon 14 and archeological ages, Christian and Intelligent Design discussion of source, measurement, results, interpretation, and errors in Carbon-14 dating.
MASS SPECTROMETRY IN THE FIELD OF FOOD INDUSTRYErin Davis
This is a powerpoint presentation solely to give a brief idea about the role of Mass Spectrometry (MS) which is one of the powerful analytical technique.This presentation describes the role of Mass Spectrometry in the field of food industry.These slides deals with the basic principle,working,components,detailed analysis etc.
Mike Riddle has put together a brilliant slideshow examining the truths and misnomers of dating methods currently used by scientists eager to propogate their evolutionary agenda. I hope you enjoy Mr. Riddle's slidshow as much as I have.
mass spectrometry for pesticides residue analysis- L3sherif Taha
This is the third lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (3) include: Electrospray ionization and Atmospheric pressure chemical ionization
mass spectrometry for pesticides residue analysis- L1sherif Taha
This is the first lecture in series of lectures on mass spectrometry for pesticides residue analysis. This lecture (1) include Pesticides classification, introduction to mass spectrometry, vacuum system for Agilent GC MS/ MS and AB SCIEX LC MS/ MS
The purpose of this manual is to help trainers provide training-cum-demonstration support on fish pond management techniques. It can be used to teach farmers the concepts, skills and practices of good pond management that are vital for producing economically feasible yields and solving pond management problems.
In addition, this manual is useful as a quick-reference and self-help guide for fishery facilitators, fish farmers and extension workers. It is designed to arouse the interest of fish farmers and the general public, thereby stimulating greater investment in fish farming. This simple training and demonstration manual has the potential to greatly improve the practices of fish farmers and help promote rapid rural aquaculture development.
What is pollination? What are pollinators important? This brochure provides tips for helping pollinators - they are responsible for assisting over 80% of the world's flowering plants!
National Wildlife Refuge System Coloring Bookusfws
The National Wildlife Refuge System Coloring Book was developed to introduce primary grade students to the Refuge System, which manages the world's premier system of public lands and waters set aside to conserve America's fish, wildlife and plants. The Refuge System has grown to more than 540 refuges with.
If you're looking for a fun way to watch birds and use a real Christmas tree, one way to recycle it after the holidays is to put it in the backyard and decorate it for wildlife.
The Pacific Salmon and Steelhead Coloring Bookusfws
Five kinds of salmon live in rivers along the Pacific Coast: the chinook (or king salmon), the coho (or silver salmon), the sockeye, pink (or humback) and chum (or dog) salmon. This coloring book talks about these fish and the steelhead (which is a type of rainbow trout) and is designed for anyone interested in learning about fish.
This coloring book aims to teach kids about fish you can catch and other animals that live in the aquatic environment. The aquatic environment is home to animals that live in water and on land. Some of these include fish, frogs and herons. Water is essential to all life.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
The ASGCT Annual Meeting was packed with exciting progress in the field advan...
Fish Identification Course
1. Date
November 16 – November 20, 2015
Location
NCTC, Shepherdstown, WV
Instructors
Stuart Welsh (WVU)
Dan Cincotta (WVDNR)
Who Should Attend
Natural resource professionals needing fish
identification skills for tasks such as
ecological research projects, predator-prey
studies, or assemblage-level biomonitoring
using fish.
Course Length
4.5 days
College Credit
2 semester hours
Tuition
Tuition for FWS, NPS, and BLM is prepaid.
Tuition is $1,195.00 for participants from
other agencies and organizations.
To Register
DOI employees: Log In to DOI Learn, enter
the course title in the search box, click
scheduled classes, click submit request.
Non-DOI employees: If you do not have a
DOI Learn account, please contact Matthew
Patterson for instructions on how to create an
account.
Course Contact
Matthew Patterson, 304/876-7473
matthew_patterson@fws.gov
Fish Identification
CSP 2220
Course Description
The purpose of this course is to develop participant fish identification skills and
knowledge of regional freshwater fish species. Participants will learn an overall
system for identifying fish. Characteristics of major taxonomic groups within each
family will provide the basis to approach species-level identification. Although
emphasis will be placed on the families Cyprinidae, Percidae, Centrarchidae,
Catostomidae, and Ictaluridae, specimens from 25 North American freshwater fish
families will be available for study. This course is "hands on" and lab-intensive.
Field exercises will provide fresh specimens for identification.
Objectives
Upon completion of this course, you will be able to:
Identify unknown fish by following methods as described in the course;
Use proper fish collection labeling and preservation techniques;
Discuss the benefits of using a combination of reference sources for fish
identification;
Use distributional maps as an aid to fish identification;
Use dichotomous keys;
Discuss identification tricks-of-the- trade; and
Correctly obtain morphometric information needed for fish identification.
Matthew Patterson (USFWS)
Student Comments about Fish ID
“This class changed the methodology that we
use to monitor our eel restoration sites. After
taking this class we feel much more confident
in our ability to ID fish.”
“Fantastic course, the instructors were
excellent”
“Great balance of lecture, lab and field
components”