PCR BASED MOLECULARMARKERS

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PRESNTED BY • •

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940C 2 min 400C -600C 1 min 700C 2 min Progressive addition of nucleotides to the free 3’-OH groups of the primers.

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ﬁ     Leaf samples collected fromdifferent plants

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Isolation of DNA Keep the tubesin PCR Thermocycler DNA strands separated Annealing of primer (36°C) 05 06 Complemenary strand synthesis 07 08 Band detected by Ethidium bromide staining Primer annealed to template DNA strands Amplified products separated by gel electrophoresis 01 02 03 04 Denature the DNA DNA Synthesis 35 to 45 cycles

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• fi fifi 4–6 nt as primer. • fi • uses less stringent conditions for annealing and primer extension • • Short extension times fi •

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• 18–32 ntfi • fi mismatches • • fi

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RAPD    AP- PCR   

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ﬁ

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• ﬁ • • ﬁ • •

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   fi fi fl

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 ﬂ   

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 

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Advantages     Disadvantages  Applications   

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    Primer elements:     

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PCR BASED MOLECULAR MARKERS

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