This document provides an overview and introduction to Pathway Studio, a software tool that helps researchers understand disease biology. It describes how Pathway Studio addresses challenges across the research and development value chain from discovery to post-launch. It highlights how Pathway Studio utilizes a large knowledgebase derived from literature to provide pathways, networks, and tools for visualizing and analyzing experimental omics data. Examples of how Pathway Studio can be used for tasks like target discovery, biomarker discovery, and drug repurposing are also presented.