Particle Size Analyses of Polydisperse Liposome Formulations with Multispectral Nanoparticle Tracking Analysis
•
3 likes•294 views
During this webinar, Dr. Singh will discuss the significance of liposome size characteristics in medicine. He will discuss the challenges present in particle size measurement for heterogeneous size containing formulation (polydisperse). He will also discuss his recently published results on polydisperse bead and liposome formulations using DLS, conventional NTA, laser diffraction and a novel multispectral NTA measurement techniques. View recorded webinars: http://bit.ly/particlewebinars
Recommended
Recommended
More Related Content
What's hot
What's hot (20)
Similar to Particle Size Analyses of Polydisperse Liposome Formulations with Multispectral Nanoparticle Tracking Analysis
Similar to Particle Size Analyses of Polydisperse Liposome Formulations with Multispectral Nanoparticle Tracking Analysis (20)
More from HORIBA Particle
More from HORIBA Particle (20)
Recently uploaded
Recently uploaded (20)
Particle Size Analyses of Polydisperse Liposome Formulations with Multispectral Nanoparticle Tracking Analysis
- 1. The views expressed are those of the authors and should not be construed to represent the positions of the U.S. Army or the DoD. Particle Size Analyses of Polydisperse Liposome Formulations with Multispectral Nanoparticle Tracking Analysis Pushpendra Singh October 16, 2019
- 2. 2 Outline Liposomes and their application in medicine Liposome manufacturing process Significance of particle size distribution for liposomes Challenges in particle size characterization
- 3. 3 Sercombe et al., Front. Pharmacol. 2015, 6: 286 Heavily being used in pharmaceutical industry as drug delivery system and vaccine Liposomes in Medicine
- 4. Liposomes in Medicine 4 Specialized delivery vehicles that mimic biological membranes Shield a drug from degradation and increase its lifespan in circulation Possess the ability of encapsulation of hydrophilic drug as well as capability of solubilizing lipophilic drug Modulate pharmacokinetics and biodistribution of drug to minimize side effects
- 6. Drying of Lipids Lipid Hydration Downsizing of Lipid Vesicles Liposome Manufacturing Process
- 7. 7 Sharma and Verma , Int. J. Green Pharm. 2017, 6: 286 Lipids in organic solvent Rotary Evaporator Aqueous Solution Dried Lipid Film Agitation MLVs LUVs/SUVs Liposome Manufacturing Process
- 8. 8 Sharma and Verma , Int. J. Green Pharm. 2017, 6: 286 Lipids in organic solvent Rotary Evaporator Aqueous Solution Dried Lipid Film Agitation MLVs LUVs/SUVs Liposome Manufacturing Process
- 9. 9 Sharma and Verma , Int. J. Green Pharm. 2017, 6: 286 Lipids in organic solvent Rotary Evaporator Aqueous Solution Dried Lipid Film Agitation MLVs LUVs/SUVs Liposome Manufacturing Process
- 10. 10 Sharma and Verma , Int. J. Green Pharm. 2017, 6: 286 Lipids in organic solvent Rotary Evaporator Aqueous Solution Dried Lipid Film Agitation MLVs LUVs/SUVs Liposome Manufacturing Process
- 11. 11 Sharma and Verma , Int. J. Green Pharm. 2017, 6: 286 Lipids in organic solvent Rotary Evaporator Aqueous Solution Dried Lipid Film Agitation MLVs LUVs/SUVs Downsizing Liposome Manufacturing Process
- 12. 12 Sharma and Verma , Int. J. Green Pharm. 2017, 6: 286 Lipids in organic solvent Rotary Evaporator Aqueous Solution Dried Lipid Film Agitation MLVs LUVs/SUVs Downsizing Filtration Liposome Manufacturing Process
- 15. Hydrated multilamellar vesicles Small size liposomes Liposome Size Distribution http://www.horiba.com
- 16. Liposomes in Vaccines 16 Adjuvant: Enhance and direct the adaptative immune response to vaccine antigens Our lab created Army Liposome Formulation (ALF) that contains various immunostimulants (e.g., Lipid A, QS21 and Aluminum salt)
- 17. Adapted from Pasquale et al., Vaccine 2015, 3: 320-343 Development of vaccine design 17 Live Pathogen Attenuated Pathogen Recombinant Immunogen
- 18. Licensed Vaccines 2017 Shingrix 18 Adapted from Pasquale et al., Vaccine 2015, 3: 320-343
- 19. Experimental vaccine: Malaria Ricin Ebola Anthrax HIV Dengue Cancer Human Studies Vaccines: 14 Phase I & 2 Phase II Malaria HIV Cancer Meningococcus ALF in Vaccines
- 20. Dimyristoyl phosphatidylcholine (DMPC) Dimyristoyl phosphatidylglycerol (DMPG) Cholesterol Monophosphoryl lipid A (MPLA) Core composition of ALF
- 21. ALF: Liposomes with MPLA ALFQ: ALF + QS21 ALFA: ALF + aluminum salt ALFQA: ALFQ + aluminum salt Types of ALF
- 22. Rationale To establish a stand-alone method for particle size characterization of polydisperse liposomal adjuvants
- 23. Why care about particle size?
- 24. Why care about particle size? Moyer et al., J Clin. Invest. 2016,126: 799-808 Biodistribution and Kinetics
- 25. Moyer et al., J Clin. Invest. 2016,126: 799-808 Biodistribution and Kinetics Why care about particle size?
- 26. Moyer et al., J Clin. Invest. 2016,126: 799-808 ProcessivityBiodistribution and Kinetics Why care about particle size?
- 27. ALF: size vs. immune response EndpointTiter Beck et al., J. Control Release 2018, 275: 12-19 Monodisperse Polydisperse
- 28. Challenges of particle sizing methods Dynamic Light Scattering (DLS) Laser Diffraction (LD) Nanoparticle Tracking Analysis (NTA) Autocorrelation of scattered intensities to deduce average particle size -Range 30 -10000 nm -does not work on polydisperse sample Narrow and wide angle scattering to deduce average particle size -Range 10 nm - 5 mm -does not work on polydisperse sample Tracking of each particle undergoing Brownian (random) motion to determine particle size -Small size range 30 - 1000 nm -works on polydisperse sample
- 29. ViewSizer
- 30. Manzo and Garcia-Parajo Rep. Prog. Phys. 2015, 78: 124601 Ernst and Kohler Phys. Chem. Chem. Phys., 2013,15: 845-849 How does it work?
- 31. Particle size distribution by ViewSizer
- 32. Alone Mix Singh et al., Int. J. Pharm. 2019, 566: 680-686 Size analysis of standards DLS LD NTA MANTA PSL beads ….. 70 nm ----- 200 nm .-.-. 600 nm 1300 nm
- 33. Alone Mix PSL beads ….. 70 nm ----- 200 nm .-.-. 600 nm 1300 nm DLS LD NTA MANTA Size analysis of standards Singh et al., Int. J. Pharm. 2019, 566: 680-686
- 34. Alone Mix PSL beads ….. 70 nm ----- 200 nm .-.-. 600 nm 1300 nm DLS LD NTA MANTA Size analysis of standards Singh et al., Int. J. Pharm. 2019, 566: 680-686
- 35. Alone Mix PSL beads ….. 70 nm ----- 200 nm .-.-. 600 nm 1300 nm DLS LD NTA MANTA Size analysis of standards Singh et al., Int. J. Pharm. 2019, 566: 680-686
- 36. Liposomes Lwet: Uniform (monodisperse) small sized liposomes manufactured by microfluidization Llyo: Heterogenous (polydisperse) sized liposomes nano to micron size 36
- 37. Particle size distribution by ViewSizer
- 38. Linear range of sensitivity LYO
- 40. Liposome size distribution in Lwet
- 41. Liposome size distribution in Llyo
- 42. DLS LD NTA MANTA ------ Lwet Llyo Size analysis of liposomes Singh et al., Int. J. Pharm. 2019, 566: 680-686
- 43. DLS LD NTA MANTA ------ Lwet Llyo Singh et al., Int. J. Pharm. 2019, 566: 680-686 Size analysis of liposomes
- 44. Summary Multispectral Nanoparticle Tracking Analysis can measure particle size 50 - 2000 nm in polydisperse liposomes Lyophilized liposome formulation contain >90% particles in the range of 500 - 1500 nm
- 45. Acknowledgements LAAR Gary Matyas Zoltan Beck WRAIR Nelson Michael Robert Gramzinski LTC(P) Julie Ake HJF Sandhya Vasan Merlin Robb HORIBA Jeffrey Bodycomb Kuba Tatarkiewicz ANATOM Bill Travers Sean Travers
- 46. Publication Singh, P., Matyas, G.R., Beck, Z. (2019) Particle Size Analyses of Polydisperse Liposome Formulations with a Novel Advanced Nanoparticle Tracking Technology. Int. J. Pharm. 566: 680-686. doi: 10.1016/j.ijpharm.2019.06.013.
- 47. Thank You
- 48. www.mantainc.com Particle size distribution by ViewSizer
- 49. Particle size distribution by ViewSizer www.mantainc.com