This document discusses personalized cancer vaccines created using neoantigens unique to a patient's tumor. The process involves procuring tumor cells from the patient for genetic sequencing to identify neoantigens. Predicted mutated peptides likely to bind the patient's HLA proteins are then synthesized into a personalized vaccine administered to the patient to trigger an immune response against tumors containing the same neoantigens. The company discussed offers DNA/RNA sequencing, neoantigen prediction and personalized vaccine manufacturing services.

2. Personalized Vaccine
？
The personalized vaccines are created based on
specific markers on a patient's tumor called
neoantigens.

3. A first class of potential cancer rejection antigens is formed by nonmutated proteins to which T cell
tolerance is incomplete-for instance, because of their restricted tissue expression pattern.
A second class of potential cancer rejection antigens is formed by peptides that are entirely absent
from the normal human genome, so-called neoantigens.
Neoantigens
？

4. How to make Personalized Vaccine
？
Tumour
procurement
Target
selection
Personal vaccine
manufacture
Vaccine
administration

5. Tumour
procurement
Take tumour cells from the patient;
These cells will be used for genetic sequencing

6. Target
selection
1. Extract DNA from tumour cells
2. Compare it with the DNA from healthy cells to see which chunks have changed
3. Figure out which ones contain instructions to make neoantigens
4. Predicted which mutated peptides were likely to bind autologous HLA-A or HLA-B
proteins of the patient

7. Synthesis clinical-grade long peptides targeting up to 20
neoantigens per patient
Personal vaccine
manufacture

8. Services we offer:
• DNA and RNA sequencing of peripheral blood mononuclear cells of a cancer patient for
detection of tumor-specific mutations.
• Translation of these mutations to human leukocyte antigen (HLA) molecules.
• Prediction of personalized peptides with high affinity binding to HLA molecules.
• Manufacture of personalized vaccine with pools of these synthetic personalized peptides.

9. Our advantages:

10. Address: 45-16 Ramsey Road, Shirley,
NY 11967, USA
Email: info@creative-peptides.com