Peptide chemists have a myriad of approaches available to optimize lead peptide structures for activity, potency and the desired selectivity for the target of interest. Thus multiple modifications and/or longer-range structural features (e.g. cyclization) are often necessary to obtain the desired stability. For example, while gonadotropin releasing hormone (GnRH) already contains pyroglutamic acid at the N-terminus and a C-terminal amide, clinically used analogs contain a D-amino acid at position 6 in the middle of the peptide to stabilize the peptides to metabolism as well as modified C-termini.

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Peptide Modification
Services
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Peptide Modification
1. Peptide Modification
2. Available Modifications
3. Application
4. Our Services

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Peptide Modification
Peptide modification refers to the covalent and
generally enzymatic modification of proteins following
protein biosynthesis. Proteins are synthesized by
ribosomes translating mRNA into polypeptide chains,
which may then undergo peptide modification to form
the mature protein product.

4. Available Modifications
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C-Terminal Modification
N-Terminal Modification
Unusual & Non-natural Amino Acids Modification
Peptide PEGylation
Fluorescent Dye/FRET pairs Modification
Post-translational Modification
Cyclic Peptides
Custom Labeling
Peptide Conjugation
Peptide Tags

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N-Terminal Modification
Acetyl
Formyl
Pyroglutamyl
(pGlu)
Fatty acids
Urea
Carbamate
Sulfonamide
Alkylamine

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C-Terminal Modification
Amides (NH2)
Aldehydes
N-alkyl amides
Esters
p-Nitroanilide (pNA) 7-Amino-4-Methylcoumarin (AMC)

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Unusual & Non-natural Amino
Acids Modification
D-amino acids
Homo-amino acids
β-homo-amino acids
N-methyl amino acids
α-methyl amino acids
Unusual amino acids

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Peptide PEGylation
PEG Carrier
Mini-PEG1 (8-Amino-3,6-dioxaoctanoic acid, AEEA)
PEG Spacer
Mini-PEG2 (12-amino-4,7,10-trioxadodecanoic acid)
Mini-PEG3 (15-amino-4,7,10,13-tetraoxapenta-decanoic acid)

9. Fluorescence and Dye Labeled
Peptides
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Post-translational Modification
Phosphorylation
Sulfation
Glycosylation
Hydroxylation
Acetylation
Prenylation

11. Other Peptide Modification
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Cyclic Peptides
•Backbone Cyclization
•Side-chain Cyclization
•CLIPS Cyclization
•Disulfide-rich Peptides
Custom Labeling
•Stable isotope labeled
•Photo cross linkers
•Spin labels
•Linkers & Spacers
Custom Conjugation
•Biotinylation
•Horseradish Peroxidase
•Gold/Silver Nanoparticles
•Magnetic Beads
Peptide Tags
•Flag tag (DYKDDDDK)
•HA tag (YPYDVPDYA)
•His tag (HHHHHH)
•Myc tag (EQKLISEEDL)

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Application
Amidation not only enhances the activity of peptide hormones,
but also prolongs their shelf life.
This increases their ability to enter cells.
Alter structure to better understand biological function
Enhance immunogenicity for antibody development and production
The changes reduce the effect of charged C- or N-termini during ELISA
binding.

13. Creative Peptides
Creative
Peptides
 We provide a variety of modified peptides for
proteomics and epigenetics.
 In addition, we will make appropriate modifications
to the peptide according to our customer's needs.
Peptide Synthesis Peptide Analysis
Peptide Design
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Our Advantages

15. LOGO
www.creative-peptides.com
Address: 45-16 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-624-4882
Fax: 1-631-614-7828
E-Mail: info@creative-peptides.com