This study evaluated the potential of gene therapy using an adeno-associated virus (AAV) vector encoding interleukin-1 receptor antagonist (IL-1Ra) for the treatment of experimental uveitis. Rabbits were injected intravitreally with the recombinant AAV vector expressing human IL-1Ra (rAAV-IL-1Ra) and expression of the transgene was detected for at least 100 days. Rabbits that received rAAV-IL-1Ra showed suppression of uveitis when experimentally induced at both 10 and 100 days after injection, demonstrating the potential for long-term treatment of uveitis with this gene therapy approach.
Genome editing as a tool for enhancing disease resistance in crops - Vladimir...OECD Environment
CRISPR-Cas genome editing can be used to engineer disease resistance in crops by targeting susceptibility genes. The document discusses using CRISPR to knock out the S-gene SlMLO1 in tomato, conferring resistance to powdery mildew. It also describes improving resistance to RNA and DNA viruses in Nicotiana benthamiana by targeting viral sequences or genes involved in viral infection. CRISPR allows generating mutations without transgenes for non-GM disease resistant crops.
This document describes a study examining a synthetic small molecule analogue (SMA-12b) of an immunomodulatory parasitic worm product (ES-62) and its ability to treat experimental arthritis. The key findings are:
1) SMA-12b prevents and treats collagen-induced arthritis in mice by modifying the expression of inflammatory genes, particularly inhibiting IL-1β production.
2) SMA-12b increases the expression of antioxidant response genes regulated by the transcription factor NRF2.
3) SMA-12b is unable to inhibit IL-1β expression in macrophages derived from NRF2-deficient mice, suggesting it acts through NRF2-mediated mechanisms.
Reengineering the scientific research paperAnita de Waard
Scientific papers tell stories that persuade with data. The document discusses how scientific papers have narrative structures similar to stories, with settings, characters, goals, attempts, and outcomes. It also notes that scientific papers use data and results to make their arguments and persuade readers of their conclusions.
Objective: To investigate the changes in the retina due to deltamethrin toxicity and the process in cell inflammation and apoptosis.
Study Design: Sixteen Wistar albino rats were randomly divided into two groups as control (n=8) and deltamethrin (n=8) groups. Saline was given to the control group, and 0.5 mL of 5 mg/kg deltamethrin was given to the deltamethrin group for 14 days each. Blood was collected for biochemical analysis. Retinal tissue was processed for histological examination.
Results: Compared to the control group, MDA levels were high while GSH and CAT levels were low in the deltamethrin group. Histopathological analysis showed spaces between the pigment epithelium, irregularity in the delimiting membrane, degenerated ganglion, cone and bacillus cell, pyknotic nuclei, thinned inner limitation membrane, and thickened vascular wall. The control group showed FAS expression in the pigment layer limiting membranes, in the nuclei of many cone and bacillus cells, and ganglion cells in the control group sections. In the deltamethrin group, FAS expression was observed in the inner and outer limiting membranes of the pigment epithelium, cone and bacillus cells, and ganglion cell nuclei. In the control group, negative NOS expression in the pigment epithelium and outer limiting membranes, internal limitation membrane, and ganglion cells in the cone and bacillus cell nuclei were observed. In the deltamethrin group, NOS expression was positive in the pigment epithelium, cone and bacillus, and ganglion cell nuclei.
Conclusion: We suggest that deltamethrin toxicity induced apoptotic process due to increased inflammation in the retina and may cause visual impairment as a result of neural damage.
Keywords: deltamethrin, FAS, insecticides, NOS, nitric oxide synthase, retina
Knowledge Media Panel U Toronto, Sept 30 2010Anita de Waard
The document discusses improving access to the key elements within scientific articles, which can be viewed as stories that follow common narrative structures. It suggests better identifying rhetorical elements like facts, hypotheses, and evidence through semantic annotation and linking data. This would allow exploring relationships between elements and adding external commentary and context over time.
Ranavirus was detected in several lizard species in Germany between 2011-2012, including green striped tree dragons, Asian glass lizards, green anoles, green iguanas, and bearded dragons. This represents the first detection of ranavirus in these lizard species. Sequence analysis found the viruses infecting each species were distinct yet related to other ranaviruses. The study suggests ranavirus infection may be an increasing threat to lizards and can cause disease in a variety of host species, especially when animals are transported or housed in large groups.
This study evaluated the effectiveness of crude extracts of Capsicum annum in controlling Newcastle disease virus (NDV) in indigenous chickens. 400 local chickens were divided into groups that were pre-treated with Capsicum annum extract, infected with NDV, or left untreated. Mortality rates were significantly lower in pre-treated groups (0-20%) compared to infected and untreated groups (50-60%). Antibody levels increased over time in pre-treated and vaccinated groups, conferring immunity. The study concludes that Capsicum annum extracts can effectively control NDV outbreaks if administered prophylactically before infection.
The document discusses how scientific articles tell stories through their structure and components to persuade editors, authors, and readers, similar to how speeches use components like an introduction to establish credibility. It notes that introductions in papers set up the subject and purpose, and introductions in speeches often use persuasive appeals. The document suggests that scientific papers position their subject and purpose in the introduction to persuade various audiences, just as speeches do.
Genome editing as a tool for enhancing disease resistance in crops - Vladimir...OECD Environment
CRISPR-Cas genome editing can be used to engineer disease resistance in crops by targeting susceptibility genes. The document discusses using CRISPR to knock out the S-gene SlMLO1 in tomato, conferring resistance to powdery mildew. It also describes improving resistance to RNA and DNA viruses in Nicotiana benthamiana by targeting viral sequences or genes involved in viral infection. CRISPR allows generating mutations without transgenes for non-GM disease resistant crops.
This document describes a study examining a synthetic small molecule analogue (SMA-12b) of an immunomodulatory parasitic worm product (ES-62) and its ability to treat experimental arthritis. The key findings are:
1) SMA-12b prevents and treats collagen-induced arthritis in mice by modifying the expression of inflammatory genes, particularly inhibiting IL-1β production.
2) SMA-12b increases the expression of antioxidant response genes regulated by the transcription factor NRF2.
3) SMA-12b is unable to inhibit IL-1β expression in macrophages derived from NRF2-deficient mice, suggesting it acts through NRF2-mediated mechanisms.
Reengineering the scientific research paperAnita de Waard
Scientific papers tell stories that persuade with data. The document discusses how scientific papers have narrative structures similar to stories, with settings, characters, goals, attempts, and outcomes. It also notes that scientific papers use data and results to make their arguments and persuade readers of their conclusions.
Objective: To investigate the changes in the retina due to deltamethrin toxicity and the process in cell inflammation and apoptosis.
Study Design: Sixteen Wistar albino rats were randomly divided into two groups as control (n=8) and deltamethrin (n=8) groups. Saline was given to the control group, and 0.5 mL of 5 mg/kg deltamethrin was given to the deltamethrin group for 14 days each. Blood was collected for biochemical analysis. Retinal tissue was processed for histological examination.
Results: Compared to the control group, MDA levels were high while GSH and CAT levels were low in the deltamethrin group. Histopathological analysis showed spaces between the pigment epithelium, irregularity in the delimiting membrane, degenerated ganglion, cone and bacillus cell, pyknotic nuclei, thinned inner limitation membrane, and thickened vascular wall. The control group showed FAS expression in the pigment layer limiting membranes, in the nuclei of many cone and bacillus cells, and ganglion cells in the control group sections. In the deltamethrin group, FAS expression was observed in the inner and outer limiting membranes of the pigment epithelium, cone and bacillus cells, and ganglion cell nuclei. In the control group, negative NOS expression in the pigment epithelium and outer limiting membranes, internal limitation membrane, and ganglion cells in the cone and bacillus cell nuclei were observed. In the deltamethrin group, NOS expression was positive in the pigment epithelium, cone and bacillus, and ganglion cell nuclei.
Conclusion: We suggest that deltamethrin toxicity induced apoptotic process due to increased inflammation in the retina and may cause visual impairment as a result of neural damage.
Keywords: deltamethrin, FAS, insecticides, NOS, nitric oxide synthase, retina
Knowledge Media Panel U Toronto, Sept 30 2010Anita de Waard
The document discusses improving access to the key elements within scientific articles, which can be viewed as stories that follow common narrative structures. It suggests better identifying rhetorical elements like facts, hypotheses, and evidence through semantic annotation and linking data. This would allow exploring relationships between elements and adding external commentary and context over time.
Ranavirus was detected in several lizard species in Germany between 2011-2012, including green striped tree dragons, Asian glass lizards, green anoles, green iguanas, and bearded dragons. This represents the first detection of ranavirus in these lizard species. Sequence analysis found the viruses infecting each species were distinct yet related to other ranaviruses. The study suggests ranavirus infection may be an increasing threat to lizards and can cause disease in a variety of host species, especially when animals are transported or housed in large groups.
This study evaluated the effectiveness of crude extracts of Capsicum annum in controlling Newcastle disease virus (NDV) in indigenous chickens. 400 local chickens were divided into groups that were pre-treated with Capsicum annum extract, infected with NDV, or left untreated. Mortality rates were significantly lower in pre-treated groups (0-20%) compared to infected and untreated groups (50-60%). Antibody levels increased over time in pre-treated and vaccinated groups, conferring immunity. The study concludes that Capsicum annum extracts can effectively control NDV outbreaks if administered prophylactically before infection.
The document discusses how scientific articles tell stories through their structure and components to persuade editors, authors, and readers, similar to how speeches use components like an introduction to establish credibility. It notes that introductions in papers set up the subject and purpose, and introductions in speeches often use persuasive appeals. The document suggests that scientific papers position their subject and purpose in the introduction to persuade various audiences, just as speeches do.
12 apple characteristics of prebreeding material kellerhals markusfruitbreedomics
This document summarizes the characteristics of apple parent lines developed by Agroscope to have resistance to multiple diseases. The lines were developed through crosses to pyramid resistances to apple scab, powdery mildew and fire blight. Seedlings were genotyped and phenotyped to select those with multiple resistance genes. Future crosses aim to combine additional traits like low chilling requirement, reduced allergenicity, and improved flavor while maintaining disease resistance and fruit quality.
Receptor binding and antigenic site analysis of hemagglutinin gene fragments ...UniversitasGadjahMada
We reported a retrospective study on hemagglutinin (HA) gene fragments of Avian Influenza (AI) viruses recovered between 2010 to 2012, using reverse transcriptase polymerase chain reaction (RT-PCR) followed by sequencing. The results provide information about the receptor binding sites (RBS) and antigenic sites character of HA gene of AI viruses in Indonesia. Viral RNA was extracted from allantoic fluid of specific pathogen free (SPF) of chicken embryonated eggs inoculated by AI suspected samples. Amplification was performed by using H5 specific primers to produce amplification target of 544 bp. The resulting sequences were analyzed with MEGA-5 consisting of multiple alignment, deductive amino acid prediction, and phylogenetic tree analysis. The results showed that out of the 12 samples amplified using RT-PCR technique, only 7 were detected to be avian influenza serotype H5 viruses. Sequence analysis of AIV H5 positive samples, showed a binding preference towards avian type receptors. Antigenic site analysis is consistent with the previous report, however, the antigenic site B at position 189 showed that the residue had undergone mutation from arginine to methionine. Phylogenetic tree analysis showed that these viruses were clustered into clade 2.1.3. Our report supports the importance of the previous study of RBS and antigenic properties of HPAI H5N1 in Indonesia.
The document summarizes the results of a summer scholarship project investigating the interactions between FZR1, an activator of the anaphase promoting complex/cyclosome, and several potential protein targets. Using techniques like immunohistochemistry, western blotting, and immunoprecipitation, the localization and interactions of PP2A-α, CDC14B, and NEDL2 were analyzed in wild type and FZR1 knockout postnatal mouse testis tissue. PP2A-α results were found to be too variable to draw conclusions. CDC14B localization differences were observed between wild type and knockout tissue, but no interaction with FZR1 was detected, though further optimization may be warranted. Improved staining of NED
This document reports on a study investigating the effect of the ABCA4 gene variant c.5461-10T/C, a frequent cause of Stargardt disease (STGD1), on mRNA splicing. The study found that in patient-derived photoreceptor progenitor cells, this variant resulted in skipping of exon 39 or exons 39 and 40 in the ABCA4 mRNA. In vitro minigene assays confirmed the variant caused these splicing defects. Patients who were homozygous for the variant displayed a severe phenotype, with early onset of STGD1, poor vision, and legal blindness by age 25. The variant was found to be located on a founder haplotype lacking other disease-causing variants,
This document summarizes research on the Scr74 gene family in Phytophthora infestans and how different variants of this gene are recognized in potato genotypes. The key points are:
1. 27 variants of the Scr74 effector gene were identified from 12 P. infestans isolates. 4 novel variants were cloned.
2. 13 amino acid sites in Scr74 were found to be under positive diversifying selection, indicating these sites are important for pathogen recognition.
3. 17 Scr74 variants were screened using PVX assays on 12 potato genotypes. Some genotypes had strong cell death responses to specific Scr74 variants, suggesting recognition of these variants.
4. Scr74 variants Scr74-A
Frizzled-4 is Required for Retinal Angiogenesis and Maintenance of the Blood-...Kim Paes
This document summarizes research on the role of Frizzled-4 (FZD4) in retinal angiogenesis and maintenance of the blood-retina barrier. Administration of a monoclonal antibody (1.99.25) that neutralizes FZD4 to wild type mice recapitulated the FZD4 knockout phenotype, inhibiting both physiological and pathological angiogenesis in the retina. Blocking FZD4 in developing retinal vessels resulted in abnormal expression of vascular markers. In adult mice, blocking FZD4 reinstated abnormal marker expression and enabled passage of molecules through the blood-retina barrier. The results demonstrate that FZD4 is required for retinal angiogenesis and maintenance of the mature blood-retina barrier.
ICAAC2008-nz2114 GP cocci F1-3962 v4.PPTVenkat Alluru
NZ2114 is a novel antimicrobial being developed to treat infections caused by Gram-positive cocci. It was tested against staphylococcal and streptococcal isolates, including resistant strains. NZ2114 demonstrated potent activity against these pathogens, with MIC50/90 generally unaffected by resistance to oxacillin, penicillin, macrolides, vancomycin, linezolid, or daptomycin. These results support the potential use of NZ2114 for treating infections where resistant staphylococci and streptococci are commonly present.
Immunogenicity of different stressed igG monoclonal antibody formulationsArchana Puranik
This study investigated the immunogenicity of different stressed monoclonal antibody formulations in transgenic mice with human immunoglobulin genes. Various stress conditions including freeze-thawing, pH shifts, heating, shaking, and metal-catalyzed oxidation were used to generate aggregates. Metal-catalyzed oxidation produced the most immunogenic formulation with the highest anti-drug antibody titers and largest number of responders. The quality of aggregates, not just their quantity or size, impacted the immune response. This study provides new insights into how different types of monoclonal antibody aggregates influence immunogenicity.
11 opportunities of germplasm for breeding peil andreasfruitbreedomics
The document discusses the history and management of fruit genetic resources. It describes how fruit genetic resource collections have existed for as long as fruit growing, with systematic preservation beginning in the early 20th century. Germplasm is assembled through donations, exchanges, and expeditions to centers of origin. It is then conserved ex-situ and characterized. Germplasm provides genetic diversity and traits for breeding to develop resilience to biotic and abiotic threats. The document highlights opportunities germplasm offers, like resistance traits, and examples of its use in apple breeding. It also describes evaluations of germplasm resistance to diseases and evaluations of quality.
In this presentation you will find everything you need to know about the antiviral drug Remdesivir.
It was made by pharmaceutical student, for medicinal chemistry course.
Hope you enjoy it and leave a like.
This document summarizes research on mechanisms of longevity and aging in C. elegans. Key findings include:
- 23-26% of longevity is genetic, with the remaining 75% due to environmental factors.
- Genes involved in proteostasis and the unfolded protein response are differentially regulated by temperature and genotype. Inhibition of certain proteostasis genes increases lifespan at 25°C but decreases it at 15°C.
- Neurodegeneration caused by RNAi of proteostasis genes starts in motor neurons with fewer synaptic connections. This suggests impaired neuronal maintenance or function at cooler temperatures.
- In wild type worms, RNAi of UPR genes hsp-3 and hsp-4
This document summarizes a study investigating the relationship between activation of the alpha7 nicotinic acetylcholine receptor (α7nAChR) and the nuclear factor erythroid 2–related factor 2 (Nrf2) in antagonizing renal ischemia-reperfusion injury. The study examined the effects of a selective α7nAChR agonist on apoptosis and apoptotic signaling in renal epithelial cells subjected to hypoxia-reoxygenation injury. It was found that the agonist attenuated apoptosis and modulated levels of apoptotic proteins like caspase-3 and Bax. Inhibition of Nrf2 partially blocked these effects, indicating Nrf2 mediates the anti-apoptotic effects of α7nAChR activation
Pilot studies were conducted on marker-assisted breeding of apples at Agroscope in Switzerland. Crosses were made between apple varieties with desired traits like disease resistance genes. Over 10,000 seedlings were screened and a marker-assisted selection was applied using newly developed SNP markers to select plants with specific disease resistance genes and fruit quality traits. Around 1,250 progeny plants from each cross were analyzed molecularly. The results showed differences between expected and observed ratios of traits, indicating the need for phenotypic validation. Selected plants were grafted and further evaluations will assess fruit quality and disease resistance.
This study uses the fruit fly Drosophila melanogaster as a model to further investigate the genetic causes of the neurological disease epilepsy. Specifically, it examines a bang-sensitive mutant known as slamdance (sda) which has been linked to a mutation in the gene CG14509. The study aims to complete the connection between CG14509 and sda by rescuing sda and other mutant alleles through transgenic expression of CG14509 guided to different neuronal populations. This helps characterize CG14509's role and localization patterns in the brain. Rescue experiments support CG14509 as the gene for sda, and indicate it has developmental impacts and localizes to optic lobes and neural tracts.
1) The study examined CD4+ T cells in the central nervous system (CNS) of mice during experimental allergic encephalomyelitis (EAE), both during active disease and remission.
2) Flow cytometry analysis revealed a 30-fold reduction in the number of CD4+ T cells in the CNS of mice in remission compared to those with active EAE.
3) However, the CD4+ T cells that remained in the CNS during remission maintained an activated memory/effector phenotype, suggesting remission is not due to downregulation of T cell function.
This document summarizes a technical session on pyramiding scab and mildew resistance genes in apple breeding at Agroscope. The objective is to cross parent lines with different resistance genes to combine two or more genes against the same pathogen. Two crosses were made between parent lines containing different resistance genes for apple scab (Rvi6 and Rvi2) and powdery mildew (Pl2). SNP markers were used to analyze the crosses and determine which resistance genes were passed to the progeny. The document demonstrates using Excel to interpret the results of the marker analysis and determine which resistance genes were combined in the progeny for future disease resistance.
Research and application of plant immunityFOODCROPS
Plant immunity can be induced or activated through primary immune responses. Research has found that elicitors such as proteins, oligosaccharides, rhizobacteria, and fungi can induce generally similar immune responses in plants, primarily involving the salicylic acid, jasmonic acid, and ethylene pathways. The immune responses of plants may differ from those of mammals and insects in not forming immune structures like IgG or IGG. Plant immunity appears to be a basic primary immune response that uses largely similar pathways to defend against pathogen invasion in a broad-spectrum or multifunctional manner.
Detection of ehrlichia platys dna in brown dog ticksJosephine Huang
This document reports on a study that detected Ehrlichia platys DNA in brown dog ticks (Rhipicephalus sanguineus) collected from dogs in Okinawa Island, Japan. Key findings include:
- Partial 16S rRNA gene sequences of E. platys were detected in 3 out of 32 ticks collected from 2 dogs using PCR and sequencing.
- This represents the first detection of E. platys in Japan and the first report of detecting it in ticks.
- Sequence analysis showed the 3 positive ticks were highly similar (99.7-100% identical) to known E. platys sequences, confirming the detection.
- This suggests R. sanguineus ticks
1) Researchers studied the internal ribosomal entry site (IRES) in the 5' untranslated region of the canine dicistrovirus (CDV-A) genome.
2) Using computational prediction and SHAPE analysis, they determined the secondary structure of the CDV-A 5'UTR IRES, which resembles the poliovirus IRES structure.
3) In vitro translation assays in rabbit reticulocyte lysate showed that the CDV-A 5'UTR IRES can direct cap-independent translation, and requires the initiation factor eIF4A.
This letter discusses a case study of a 50-year-old woman with breast cancer who developed a choroidal metastasis in her left eye. Systemic chemotherapy was ineffective at controlling the growth of the choroidal lesion. She received an intravitreal injection of bevacizumab, which led to significant regression of the choroidal tumor over a 6 month period with no recurrence over 24 months of follow up. The letter suggests intravitreal bevacizumab may be an effective treatment for choroidal metastases when systemic chemotherapy proves insufficient.
This summary provides the key points from the document in 3 sentences:
This study explored how sulforaphane affects calcium levels and cell viability in renal tubular cells. The results showed that sulforaphane increased calcium levels in the cells in a concentration-dependent manner by promoting calcium entry from outside the cell rather than calcium release from internal stores. Sulforaphane also decreased cell viability in a concentration-dependent manner, which was independent of changes in calcium levels and may have involved the induction of apoptosis.
12 apple characteristics of prebreeding material kellerhals markusfruitbreedomics
This document summarizes the characteristics of apple parent lines developed by Agroscope to have resistance to multiple diseases. The lines were developed through crosses to pyramid resistances to apple scab, powdery mildew and fire blight. Seedlings were genotyped and phenotyped to select those with multiple resistance genes. Future crosses aim to combine additional traits like low chilling requirement, reduced allergenicity, and improved flavor while maintaining disease resistance and fruit quality.
Receptor binding and antigenic site analysis of hemagglutinin gene fragments ...UniversitasGadjahMada
We reported a retrospective study on hemagglutinin (HA) gene fragments of Avian Influenza (AI) viruses recovered between 2010 to 2012, using reverse transcriptase polymerase chain reaction (RT-PCR) followed by sequencing. The results provide information about the receptor binding sites (RBS) and antigenic sites character of HA gene of AI viruses in Indonesia. Viral RNA was extracted from allantoic fluid of specific pathogen free (SPF) of chicken embryonated eggs inoculated by AI suspected samples. Amplification was performed by using H5 specific primers to produce amplification target of 544 bp. The resulting sequences were analyzed with MEGA-5 consisting of multiple alignment, deductive amino acid prediction, and phylogenetic tree analysis. The results showed that out of the 12 samples amplified using RT-PCR technique, only 7 were detected to be avian influenza serotype H5 viruses. Sequence analysis of AIV H5 positive samples, showed a binding preference towards avian type receptors. Antigenic site analysis is consistent with the previous report, however, the antigenic site B at position 189 showed that the residue had undergone mutation from arginine to methionine. Phylogenetic tree analysis showed that these viruses were clustered into clade 2.1.3. Our report supports the importance of the previous study of RBS and antigenic properties of HPAI H5N1 in Indonesia.
The document summarizes the results of a summer scholarship project investigating the interactions between FZR1, an activator of the anaphase promoting complex/cyclosome, and several potential protein targets. Using techniques like immunohistochemistry, western blotting, and immunoprecipitation, the localization and interactions of PP2A-α, CDC14B, and NEDL2 were analyzed in wild type and FZR1 knockout postnatal mouse testis tissue. PP2A-α results were found to be too variable to draw conclusions. CDC14B localization differences were observed between wild type and knockout tissue, but no interaction with FZR1 was detected, though further optimization may be warranted. Improved staining of NED
This document reports on a study investigating the effect of the ABCA4 gene variant c.5461-10T/C, a frequent cause of Stargardt disease (STGD1), on mRNA splicing. The study found that in patient-derived photoreceptor progenitor cells, this variant resulted in skipping of exon 39 or exons 39 and 40 in the ABCA4 mRNA. In vitro minigene assays confirmed the variant caused these splicing defects. Patients who were homozygous for the variant displayed a severe phenotype, with early onset of STGD1, poor vision, and legal blindness by age 25. The variant was found to be located on a founder haplotype lacking other disease-causing variants,
This document summarizes research on the Scr74 gene family in Phytophthora infestans and how different variants of this gene are recognized in potato genotypes. The key points are:
1. 27 variants of the Scr74 effector gene were identified from 12 P. infestans isolates. 4 novel variants were cloned.
2. 13 amino acid sites in Scr74 were found to be under positive diversifying selection, indicating these sites are important for pathogen recognition.
3. 17 Scr74 variants were screened using PVX assays on 12 potato genotypes. Some genotypes had strong cell death responses to specific Scr74 variants, suggesting recognition of these variants.
4. Scr74 variants Scr74-A
Frizzled-4 is Required for Retinal Angiogenesis and Maintenance of the Blood-...Kim Paes
This document summarizes research on the role of Frizzled-4 (FZD4) in retinal angiogenesis and maintenance of the blood-retina barrier. Administration of a monoclonal antibody (1.99.25) that neutralizes FZD4 to wild type mice recapitulated the FZD4 knockout phenotype, inhibiting both physiological and pathological angiogenesis in the retina. Blocking FZD4 in developing retinal vessels resulted in abnormal expression of vascular markers. In adult mice, blocking FZD4 reinstated abnormal marker expression and enabled passage of molecules through the blood-retina barrier. The results demonstrate that FZD4 is required for retinal angiogenesis and maintenance of the mature blood-retina barrier.
ICAAC2008-nz2114 GP cocci F1-3962 v4.PPTVenkat Alluru
NZ2114 is a novel antimicrobial being developed to treat infections caused by Gram-positive cocci. It was tested against staphylococcal and streptococcal isolates, including resistant strains. NZ2114 demonstrated potent activity against these pathogens, with MIC50/90 generally unaffected by resistance to oxacillin, penicillin, macrolides, vancomycin, linezolid, or daptomycin. These results support the potential use of NZ2114 for treating infections where resistant staphylococci and streptococci are commonly present.
Immunogenicity of different stressed igG monoclonal antibody formulationsArchana Puranik
This study investigated the immunogenicity of different stressed monoclonal antibody formulations in transgenic mice with human immunoglobulin genes. Various stress conditions including freeze-thawing, pH shifts, heating, shaking, and metal-catalyzed oxidation were used to generate aggregates. Metal-catalyzed oxidation produced the most immunogenic formulation with the highest anti-drug antibody titers and largest number of responders. The quality of aggregates, not just their quantity or size, impacted the immune response. This study provides new insights into how different types of monoclonal antibody aggregates influence immunogenicity.
11 opportunities of germplasm for breeding peil andreasfruitbreedomics
The document discusses the history and management of fruit genetic resources. It describes how fruit genetic resource collections have existed for as long as fruit growing, with systematic preservation beginning in the early 20th century. Germplasm is assembled through donations, exchanges, and expeditions to centers of origin. It is then conserved ex-situ and characterized. Germplasm provides genetic diversity and traits for breeding to develop resilience to biotic and abiotic threats. The document highlights opportunities germplasm offers, like resistance traits, and examples of its use in apple breeding. It also describes evaluations of germplasm resistance to diseases and evaluations of quality.
In this presentation you will find everything you need to know about the antiviral drug Remdesivir.
It was made by pharmaceutical student, for medicinal chemistry course.
Hope you enjoy it and leave a like.
This document summarizes research on mechanisms of longevity and aging in C. elegans. Key findings include:
- 23-26% of longevity is genetic, with the remaining 75% due to environmental factors.
- Genes involved in proteostasis and the unfolded protein response are differentially regulated by temperature and genotype. Inhibition of certain proteostasis genes increases lifespan at 25°C but decreases it at 15°C.
- Neurodegeneration caused by RNAi of proteostasis genes starts in motor neurons with fewer synaptic connections. This suggests impaired neuronal maintenance or function at cooler temperatures.
- In wild type worms, RNAi of UPR genes hsp-3 and hsp-4
This document summarizes a study investigating the relationship between activation of the alpha7 nicotinic acetylcholine receptor (α7nAChR) and the nuclear factor erythroid 2–related factor 2 (Nrf2) in antagonizing renal ischemia-reperfusion injury. The study examined the effects of a selective α7nAChR agonist on apoptosis and apoptotic signaling in renal epithelial cells subjected to hypoxia-reoxygenation injury. It was found that the agonist attenuated apoptosis and modulated levels of apoptotic proteins like caspase-3 and Bax. Inhibition of Nrf2 partially blocked these effects, indicating Nrf2 mediates the anti-apoptotic effects of α7nAChR activation
Pilot studies were conducted on marker-assisted breeding of apples at Agroscope in Switzerland. Crosses were made between apple varieties with desired traits like disease resistance genes. Over 10,000 seedlings were screened and a marker-assisted selection was applied using newly developed SNP markers to select plants with specific disease resistance genes and fruit quality traits. Around 1,250 progeny plants from each cross were analyzed molecularly. The results showed differences between expected and observed ratios of traits, indicating the need for phenotypic validation. Selected plants were grafted and further evaluations will assess fruit quality and disease resistance.
This study uses the fruit fly Drosophila melanogaster as a model to further investigate the genetic causes of the neurological disease epilepsy. Specifically, it examines a bang-sensitive mutant known as slamdance (sda) which has been linked to a mutation in the gene CG14509. The study aims to complete the connection between CG14509 and sda by rescuing sda and other mutant alleles through transgenic expression of CG14509 guided to different neuronal populations. This helps characterize CG14509's role and localization patterns in the brain. Rescue experiments support CG14509 as the gene for sda, and indicate it has developmental impacts and localizes to optic lobes and neural tracts.
1) The study examined CD4+ T cells in the central nervous system (CNS) of mice during experimental allergic encephalomyelitis (EAE), both during active disease and remission.
2) Flow cytometry analysis revealed a 30-fold reduction in the number of CD4+ T cells in the CNS of mice in remission compared to those with active EAE.
3) However, the CD4+ T cells that remained in the CNS during remission maintained an activated memory/effector phenotype, suggesting remission is not due to downregulation of T cell function.
This document summarizes a technical session on pyramiding scab and mildew resistance genes in apple breeding at Agroscope. The objective is to cross parent lines with different resistance genes to combine two or more genes against the same pathogen. Two crosses were made between parent lines containing different resistance genes for apple scab (Rvi6 and Rvi2) and powdery mildew (Pl2). SNP markers were used to analyze the crosses and determine which resistance genes were passed to the progeny. The document demonstrates using Excel to interpret the results of the marker analysis and determine which resistance genes were combined in the progeny for future disease resistance.
Research and application of plant immunityFOODCROPS
Plant immunity can be induced or activated through primary immune responses. Research has found that elicitors such as proteins, oligosaccharides, rhizobacteria, and fungi can induce generally similar immune responses in plants, primarily involving the salicylic acid, jasmonic acid, and ethylene pathways. The immune responses of plants may differ from those of mammals and insects in not forming immune structures like IgG or IGG. Plant immunity appears to be a basic primary immune response that uses largely similar pathways to defend against pathogen invasion in a broad-spectrum or multifunctional manner.
Detection of ehrlichia platys dna in brown dog ticksJosephine Huang
This document reports on a study that detected Ehrlichia platys DNA in brown dog ticks (Rhipicephalus sanguineus) collected from dogs in Okinawa Island, Japan. Key findings include:
- Partial 16S rRNA gene sequences of E. platys were detected in 3 out of 32 ticks collected from 2 dogs using PCR and sequencing.
- This represents the first detection of E. platys in Japan and the first report of detecting it in ticks.
- Sequence analysis showed the 3 positive ticks were highly similar (99.7-100% identical) to known E. platys sequences, confirming the detection.
- This suggests R. sanguineus ticks
1) Researchers studied the internal ribosomal entry site (IRES) in the 5' untranslated region of the canine dicistrovirus (CDV-A) genome.
2) Using computational prediction and SHAPE analysis, they determined the secondary structure of the CDV-A 5'UTR IRES, which resembles the poliovirus IRES structure.
3) In vitro translation assays in rabbit reticulocyte lysate showed that the CDV-A 5'UTR IRES can direct cap-independent translation, and requires the initiation factor eIF4A.
This letter discusses a case study of a 50-year-old woman with breast cancer who developed a choroidal metastasis in her left eye. Systemic chemotherapy was ineffective at controlling the growth of the choroidal lesion. She received an intravitreal injection of bevacizumab, which led to significant regression of the choroidal tumor over a 6 month period with no recurrence over 24 months of follow up. The letter suggests intravitreal bevacizumab may be an effective treatment for choroidal metastases when systemic chemotherapy proves insufficient.
This summary provides the key points from the document in 3 sentences:
This study explored how sulforaphane affects calcium levels and cell viability in renal tubular cells. The results showed that sulforaphane increased calcium levels in the cells in a concentration-dependent manner by promoting calcium entry from outside the cell rather than calcium release from internal stores. Sulforaphane also decreased cell viability in a concentration-dependent manner, which was independent of changes in calcium levels and may have involved the induction of apoptosis.
Analisis procesos ligeros vs pesados resumidoOscar Limachi
El documento compara procesos ágiles y pesados, describiendo características del PUDS y Scrum. PUDS divide el proceso en 4 fases iterativas con flujos de trabajo repetitivos, mientras que Scrum se enfoca en incrementos demostrables mensuales con comunicación entre el equipo y cliente. También resume Microsoft Solutions Framework, Microsoft Operations Framework e ITIL, marcos enfocados en entregar soluciones de TI exitosas siguiendo principios como comunicación abierta, enfoque en valor de negocio y aprendizaje continuo.
The document discusses demand and supply. It states that demand is the inverse relationship between price and quantity demanded, while supply is the direct relationship between price and quantity supplied. The document includes graphs that illustrate the demand curve, which slopes downward, and the supply curve, which slopes upward. It also shows how market equilibrium is reached at the price and quantity where the supply and demand curves intersect.
The document provides information about cochlear implants, including:
1) Cochlear implants bypass damaged parts of the inner ear to electrically stimulate the auditory nerve. They allow access to sound and can help develop speech recognition and communication.
2) The history of cochlear implants dates back to the 18th century but modern multi-channel implants were developed in the 1980s and have improved performance.
3) Over 100,000 people worldwide have received cochlear implants, including over 6,000 procedures annually in the US. The number of recipients is growing as criteria have expanded to include milder and unilateral hearing losses.
Waterproofing concrete roof by Astec Re-Ply SystemShakil Chowdhury
The 50 years old concrete roof of BAT Bangladesh was leaking, and leaking in spite of numerous efforts by its maintenance division. Their latest step of 'roofing felt' too failed to control the leaking. Astec Re-Ply System was installed by its authorized applicator in Bangladesh (Rafsha BD Ltd) in December 2012. The project management was very happy with the work because 'the problem roof' never leaked again till date. The presentation unfolds the waterproofing work in BAT Bangladesh. It was a tough job, but done easily.
Astec Re-Ply restores Viyellatex Roof in BangladeshShakil Chowdhury
Eco Couture Ltd is RMG factory in Bangladesh. Its' 75000 SFT metal roof was leaking and spreading corrosion. They wanted Astec Re-Ply System to restore the roof. The presentation unveils how the work was accomplished by Rafsha Bangladesh Ltd. Rafsha is the global partner of Insulating Coatings Corporation of USA and the authorized applicator of ASTEC product in Bangladesh.
El documento proporciona tres formas de contactar a Bioestética JC: a través de su sitio web www.bioesteticajc.com, por correo electrónico a Pedidos@bioesteticajc.com o Contactanos@bioesteticajc.com, o llamando al número de teléfono PBX (6) 8906589 en Manizales, Colombia.
Este documento presenta una propuesta de negocio para desarrollar un shampoo innovador llamado "Fambúúú" para limpiar vehículos. El shampoo protegerá los vehículos de la lluvia, el polvo y la suciedad, manteniéndolos limpios sin importar las condiciones climáticas. El mercado objetivo es Bucaramanga y su área metropolitana, donde se espera captar al 1% del mercado de propietarios de vehículos. Se requiere una inversión inicial de $78.200.000 pesos
Novel research aimed at finding a cure for AIDS requires animal models responding to human antiretroviral drugs. However, there have been few antiretrovirals cross-active against the simian viruses. In this study, we expanded the arsenal of drugs active against the simian retrovirus SIVmac251 and showed that this virus is inhibited by the protease inhibitor, darunavir, and the CCR5 blocker, maraviroc. Administration of these two drugs in combination with the reverse transcriptase inhibitors, tenofovir and emtricitabine, and the integrase inhibitor, raltegravir, resulted in prolonged plasma viral loads below assay detection limits, and, surprisingly, restricted the viral reservoir, a marker of which is viral DNA. We then decided to employ this multidrug regimen (termed “highly intensified ART”) in order to increase the potency of a previous strategy based on the gold drug auranofin, which recently proved able to restrict the viral reservoir in vivo. A short course of highly intensified ART following the previous treatment resulted, upon therapy suspension, in a remarkably spontaneous control of the infection, that may pave the way to a persistent suppression of viremia in the absence of ART. These results corroborate the robustness of the macaque AIDS model as a vanguard for potentially future treatments for HIV in humans.
Gene therapy with recombinant adeno-associated vectors was tested for neovascular age-related macular degeneration in a phase 1 clinical trial. Nine patients were enrolled and randomly assigned to receive either a low dose (3 patients) or high dose (3 patients) of rAAV.sFLT-1 gene therapy, or no treatment (2 control patients). The gene therapy was delivered via subretinal injection and was found to be safe and well tolerated with no drug-related adverse events. Patients receiving gene therapy required fewer rescue injections of ranibizumab over the one-year follow-up period compared to control patients, suggesting rAAV.sFLT-1 may provide long-term treatment effects for
Gene transfer in the liver using recombinant adeno-associated virusJonathan G. Godwin
This document describes methods for delivering gene transfer vectors to the liver using recombinant adeno-associated virus (rAAV). It discusses that intravenous injection of rAAV serotypes results in efficient transduction of the liver. The document provides protocols for preparing rAAV vector samples and delivering the vectors to mouse liver through lateral tail vein injection, retro-orbital sinus injection, or portal vein injection. It notes that tail vein injection is the most common method but retro-orbital sinus injection can be used as an alternative for young or neonatal mice. Precise vector dosing and injection technique are emphasized for achieving effective liver-directed gene transfer.
The document discusses malaria, which infects hundreds of millions annually and kills over 1 million people per year, mostly in Africa. It outlines the challenges in developing an effective malaria vaccine, including the parasite's ability to evade the immune system and lack of animal models for testing. Several past and current vaccine candidates are mentioned, including SPf66 (the first field trial vaccine), RTS,S (the most advanced candidate to date), and PfSPZ Vaccine (a whole parasite vaccine showing promise in recent trials). Overall, the document reviews the state of malaria vaccine research and the hurdles remaining in developing a highly effective vaccine.
This document summarizes a study that evaluated the potential of an adeno-associated virus (AAV) vector delivering a peptide derived from the Nrf2 protein to target the Nrf2 signaling pathway in the retina. The Nrf2 peptide was fused to a cell-penetrating peptide sequence (Tat-peptide) and expressed from an AAV vector. In vitro, the TatNrf2mer peptide induced antioxidant gene expression, blocked IL-1β secretion, and protected cells from oxidative injury. In mouse models, TatNrf2mer expression partially protected photoreceptor function and decreased inflammatory cytokines and cells in models of retinal oxidative injury and uveitis. The results suggest this AAV-delivered TatNrf
The document summarizes a study on the protein profiles of gamma ray irradiated blood stages of Plasmodium berghei, the malaria parasite, for developing a malaria vaccine candidate. Key findings include:
1) Protein profiles differed between infected and uninfected blood, indicating exported parasite proteins. Higher irradiation doses and dose rates resulted in more protein bands.
2) A dose of 150 Gy and dose rate of 380 Gy/hour altered protein profiles the most for vaccine development.
3) Protein profiles depended on parasite density in blood and differed between P. berghei and other rodent malaria species.
4) Profiles were unaffected by infection duration but depended on parasite composition in blood stages. Altered proteins could
1) Researchers expressed and purified the UL35 gene product (VP26) of duck plague virus (DPV) to homogeneity.
2) Polyclonal antibodies were produced in rabbits using the purified recombinant VP26 protein.
3) Western blot analysis confirmed that the polyclonal antibodies recognized the recombinant VP26 protein.
This study analyzed faecal specimens from 2,495 diarrhoea cases in Kolkata, India between 2007-2009 to determine the seasonal distribution and characteristics of norovirus (NoV) infections. NoV was detected in 78 cases, mostly children under 2 years old, sometimes as the sole pathogen but often along with other enteric pathogens. Sequencing of the NVGII strains showed clustering with GII.4, GII.13 and GII.6 NoV types. NoV infections occurred year-round and were associated with mild dehydration in children and adults in Kolkata.
Gene Olinger, USAMRIID, Fort Detrick USA, presents at the ProImmune Antigen Characterization and Biomarker Discovery Summit, January 2011.
Protective Immune Reponses to Ebola Virus
Dr. Tanja Opriessnig - Update on novel experimental pig vaccine approachesJohn Blue
Update on novel experimental pig vaccine approaches - Dr. Tanja Opriessnig, The Roslin Institute, University of Edinburgh and Iowa State University, from the 2016 North American PRRS Symposium, December 3‐4, 2016, Chicago, Illinois, USA.
More presentations at http://www.swinecast.com/2016-north-american-prrs-symposium
This document describes research on cloning, expressing, and purifying the L-type lectin LvLTLC1 from the white leg shrimp Litopenaeus vannamei. LvLTLC1 was previously shown to support shrimp innate immunity against Vibrio parahaemolyticus, the pathogen causing acute hepatopancreatic necrosis disease (AHPND). The researchers cloned the LvLTLC1 gene from L. vannamei shrimp and expressed it recombinantly in E. coli. They purified the recombinant LvLTLC1 protein and found it was able to agglutinate, or clump, V. parahaemolyticus in a calcium-independent manner. This suggests LvLTLC1
MVI is working with ICGEB in India to develop a vaccine against Plasmodium vivax. This effort includes Bharat Biotech, which will manufacture the vaccine for preclinical and initial safety trials in adults. The vaccine aims to prevent infection, decrease infection intensity, and prevent malaria transmission.
Paratuberculosis (PTB) remains one of the most obstacles limit animal breeding sector all over the world. The current study aimed to detect the etiology of PTB in tissues of clinically suspected small ruminants using histopathological and real-time polymerase chain reaction (RT-PCR) methods. Clinical examination showed 10 (26.4%) PTB suspected cases out of the total (38) examined animals. The suspected cases were euthanized, necropsied, gross lesions were recorded and tissue samples were collected for histopathological and molecular procedures. Grossly intestinal and mesenteric lymph nodes thickening, corrugations and edematous swellings were recorded. Semi-thin sections of the intestine and mesenteric lymph nodes stained with toluidine blue demonstrated MAP organism inside epithelium cells and macrophages. RT-PCR detected MAP IS900 gene in all suspected cases (100%), thus we recommend using RT-PCR as a rapid sensitive method in the diagnosis of PTB.
Key-words: Paratuberculosis, Mycobacterium, Semi thin sections, Toluidine blue, IS900 gene
This study investigated whether lower levels of eosinophil-related T helper 2 (Th2) cytokines are associated with coronary artery lesions in patients with Kawasaki disease. The researchers measured levels of interleukin (IL)-4, IL-5, eotaxin, and eosinophil cationic protein (ECP) in 95 Kawasaki disease patients before and after intravenous immunoglobulin (IVIG) treatment and compared them to 30 healthy controls. They found that higher levels of IL-5 and eosinophils after IVIG treatment were associated with a lower rate of coronary artery lesions. An increase in IL-5 and eosinophils levels after treatment, but not ECP, was inversely correlated with
This study investigated whether lower levels of eosinophil-related Th2 cytokines (IL-4, IL-5, eotaxin) and the eosinophil activation marker ECP are associated with coronary artery lesions (CAL) in patients with Kawasaki disease. The study found that:
1) KD patients had higher levels of eosinophils, IL-4, IL-5, eotaxin, and ECP compared to controls.
2) After IVIG treatment, levels of IL-4, IL-5, and eotaxin increased while ECP levels decreased.
3) Higher post-IVIG levels of eosinophils and IL-5
Prevalence of Rota Virus Detection by Reverse TranscriptasePolymerase Chain R...IOSRJPBS
The present study was conducted for the period from 1/6/2016 to 20/1/2017 in Baquba city. The study aimed to detection of rotavirus in stool specimens of children fewer than five age and also explore the effects of certain demographic factors on the detection rates by revers transcriptase- polymerase chain reaction. The study included 49 patients with acute diarrhea, 32 were male and 17 were female. The age range was two months to 5 years. Demographic information on the patients regarding age, sex, residence, type of feeding and source of drinking water were collected from their parents. Stool specimens were collected from each patients and. Detection of rotavirus in stool specimens was done by conventional reverse transcriptase polymerase chain reaction (RT-PCR). The results of present study showed that the overall infection rate by rotavirus among patients with acute diarrhea by RT-PCR tests was 93.88%. The highest infection rate was recorded among those >10-≤15 months of age. None of the results showed significantly difference between female and male, PCR (88% vs 96.87%). Likewise, there was insignificantly difference between urban and rural residence, PCR (95.65% vs 92.30%). The results revealed insignificantly higher infection rate among patients (those below 2 years) feed mixing (91.66%) and bottled (100%) compared to that breast feeding (77.77%) by RT-PCR. The rotavirus infection rate was insignificantly higher among patients consuming municipal water for drinking (97.22%) compared to those consuming bottled water (84.61%) by the RT-PCR. The study concluded that rotavirus was detected in high rates among children less than 5 years old with acute diarrhea in Baquba city, particularly those less than 2 year old.
The study found that Ivermectin, an FDA-approved anti-parasitic drug, is able to inhibit the replication of SARS-CoV-2, the virus that causes COVID-19, in vitro. A single dose of Ivermectin resulted in a 5000-fold reduction in viral RNA at 48 hours in infected Vero-hSLAM cells. The authors hypothesize that Ivermectin acts by inhibiting the nuclear import of viral proteins through interaction with importin proteins. They conclude that Ivermectin warrants further investigation for potential benefits against COVID-19 in humans.
The study found that Ivermectin, an FDA-approved drug used to treat parasites, inhibited the replication of SARS-CoV-2, the virus that causes COVID-19, in vitro. A single dose of Ivermectin resulted in a 5000-fold reduction in viral RNA at 48 hours in infected cells. The authors hypothesize this is likely through inhibiting the nuclear import of viral proteins. They conclude Ivermectin warrants further investigation for possible benefits in humans and could help limit viral load and prevent severe disease if given early in infection.
The study found that Ivermectin, an FDA-approved anti-parasitic drug, is able to inhibit the replication of SARS-CoV-2, the virus that causes COVID-19, in vitro. A single dose of Ivermectin resulted in a ~5000-fold reduction in viral RNA at 48 hours when added to infected cells. Ivermectin is believed to work by inhibiting the nuclear import of viral proteins through interaction with importin proteins. The study suggests Ivermectin warrants further investigation for potential benefits against COVID-19 in humans.
1) The document discusses OCT technology, including its principles and history of development. OCT uses low coherence interferometry to perform high-resolution cross-sectional imaging of biological tissues.
2) Time-domain OCT was initially developed but newer Fourier-domain OCT provides faster acquisition speed and higher resolution.
3) The document reviews various clinical applications of OCT in ophthalmology, including imaging of the retina, glaucoma, cornea, and cataracts. Common scanning patterns and what can be observed are described for retinal and glaucoma examination.
The clinical lab provides diagnostic test data to aid in the detection, diagnosis and treatment of disease. The lab is responsible for correct identification, collection and processing of patient specimens, accurate performance of testing, timely reporting of results, and communication with healthcare professionals. There are six main steps in how a sample flows through the lab: 1) test is ordered, 2) sample is collected, 3) sample is delivered to the lab, 4) sample is processed, 5) sample is analyzed, and 6) results are reported. Common specimen types include blood, urine, body fluids, sputum, stool, and tissue samples.
This document discusses hearing and assistive devices, from hearing aids to electronic ears. It begins by explaining the auditory system as an energy transformation system, where acoustic energy is transformed into mechanical, hydraulic, and bioelectric energy as it travels through the outer, middle, and inner ear. It then provides detailed descriptions of the anatomy and functions of the outer, middle, and inner ear. It also discusses various physical properties of sound including frequency, wavelength, amplitude, interference, resonance, and more. The document is an in-depth overview of the human auditory system and how assistive devices can help with hearing impairments.
This document is a near vision test chart that contains lines of progressively smaller letters labeled with visual acuity measurements ranging from 20/200 to 20/20. The 20/20 line contains the smallest 3 point type text that a person with normal vision can read from a distance of 35 cm, indicating excellent close-up visual acuity.
The document discusses anatomy and diseases of the eye. It describes the layers of the eyeball including the uvea and sclera. Various types of uveitis are classified and their symptoms, signs, etiologies, and treatments are explained. Specific uveitic entities like HLA-B27 associated uveitis, sarcoidosis, and toxoplasmosis are also summarized along with their diagnostic approach and management. Uveal tumors and scleral diseases are briefly covered as well.
Cosa hanno in comune un mattoncino Lego e la backdoor XZ?Speck&Tech
ABSTRACT: A prima vista, un mattoncino Lego e la backdoor XZ potrebbero avere in comune il fatto di essere entrambi blocchi di costruzione, o dipendenze di progetti creativi e software. La realtà è che un mattoncino Lego e il caso della backdoor XZ hanno molto di più di tutto ciò in comune.
Partecipate alla presentazione per immergervi in una storia di interoperabilità, standard e formati aperti, per poi discutere del ruolo importante che i contributori hanno in una comunità open source sostenibile.
BIO: Sostenitrice del software libero e dei formati standard e aperti. È stata un membro attivo dei progetti Fedora e openSUSE e ha co-fondato l'Associazione LibreItalia dove è stata coinvolta in diversi eventi, migrazioni e formazione relativi a LibreOffice. In precedenza ha lavorato a migrazioni e corsi di formazione su LibreOffice per diverse amministrazioni pubbliche e privati. Da gennaio 2020 lavora in SUSE come Software Release Engineer per Uyuni e SUSE Manager e quando non segue la sua passione per i computer e per Geeko coltiva la sua curiosità per l'astronomia (da cui deriva il suo nickname deneb_alpha).
Introducing Milvus Lite: Easy-to-Install, Easy-to-Use vector database for you...Zilliz
Join us to introduce Milvus Lite, a vector database that can run on notebooks and laptops, share the same API with Milvus, and integrate with every popular GenAI framework. This webinar is perfect for developers seeking easy-to-use, well-integrated vector databases for their GenAI apps.
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
Pushing the limits of ePRTC: 100ns holdover for 100 daysAdtran
At WSTS 2024, Alon Stern explored the topic of parametric holdover and explained how recent research findings can be implemented in real-world PNT networks to achieve 100 nanoseconds of accuracy for up to 100 days.
Dr. Sean Tan, Head of Data Science, Changi Airport Group
Discover how Changi Airport Group (CAG) leverages graph technologies and generative AI to revolutionize their search capabilities. This session delves into the unique search needs of CAG’s diverse passengers and customers, showcasing how graph data structures enhance the accuracy and relevance of AI-generated search results, mitigating the risk of “hallucinations” and improving the overall customer journey.
UiPath Test Automation using UiPath Test Suite series, part 5DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 5. In this session, we will cover CI/CD with devops.
Topics covered:
CI/CD with in UiPath
End-to-end overview of CI/CD pipeline with Azure devops
Speaker:
Lyndsey Byblow, Test Suite Sales Engineer @ UiPath, Inc.
Sudheer Mechineni, Head of Application Frameworks, Standard Chartered Bank
Discover how Standard Chartered Bank harnessed the power of Neo4j to transform complex data access challenges into a dynamic, scalable graph database solution. This keynote will cover their journey from initial adoption to deploying a fully automated, enterprise-grade causal cluster, highlighting key strategies for modelling organisational changes and ensuring robust disaster recovery. Learn how these innovations have not only enhanced Standard Chartered Bank’s data infrastructure but also positioned them as pioneers in the banking sector’s adoption of graph technology.
How to Get CNIC Information System with Paksim Ga.pptxdanishmna97
Pakdata Cf is a groundbreaking system designed to streamline and facilitate access to CNIC information. This innovative platform leverages advanced technology to provide users with efficient and secure access to their CNIC details.
Threats to mobile devices are more prevalent and increasing in scope and complexity. Users of mobile devices desire to take full advantage of the features
available on those devices, but many of the features provide convenience and capability but sacrifice security. This best practices guide outlines steps the users can take to better protect personal devices and information.
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdfMalak Abu Hammad
Discover how MongoDB Atlas and vector search technology can revolutionize your application's search capabilities. This comprehensive presentation covers:
* What is Vector Search?
* Importance and benefits of vector search
* Practical use cases across various industries
* Step-by-step implementation guide
* Live demos with code snippets
* Enhancing LLM capabilities with vector search
* Best practices and optimization strategies
Perfect for developers, AI enthusiasts, and tech leaders. Learn how to leverage MongoDB Atlas to deliver highly relevant, context-aware search results, transforming your data retrieval process. Stay ahead in tech innovation and maximize the potential of your applications.
#MongoDB #VectorSearch #AI #SemanticSearch #TechInnovation #DataScience #LLM #MachineLearning #SearchTechnology
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdfPaige Cruz
Monitoring and observability aren’t traditionally found in software curriculums and many of us cobble this knowledge together from whatever vendor or ecosystem we were first introduced to and whatever is a part of your current company’s observability stack.
While the dev and ops silo continues to crumble….many organizations still relegate monitoring & observability as the purview of ops, infra and SRE teams. This is a mistake - achieving a highly observable system requires collaboration up and down the stack.
I, a former op, would like to extend an invitation to all application developers to join the observability party will share these foundational concepts to build on:
Building RAG with self-deployed Milvus vector database and Snowpark Container...Zilliz
This talk will give hands-on advice on building RAG applications with an open-source Milvus database deployed as a docker container. We will also introduce the integration of Milvus with Snowpark Container Services.
TrustArc Webinar - 2024 Global Privacy SurveyTrustArc
How does your privacy program stack up against your peers? What challenges are privacy teams tackling and prioritizing in 2024?
In the fifth annual Global Privacy Benchmarks Survey, we asked over 1,800 global privacy professionals and business executives to share their perspectives on the current state of privacy inside and outside of their organizations. This year’s report focused on emerging areas of importance for privacy and compliance professionals, including considerations and implications of Artificial Intelligence (AI) technologies, building brand trust, and different approaches for achieving higher privacy competence scores.
See how organizational priorities and strategic approaches to data security and privacy are evolving around the globe.
This webinar will review:
- The top 10 privacy insights from the fifth annual Global Privacy Benchmarks Survey
- The top challenges for privacy leaders, practitioners, and organizations in 2024
- Key themes to consider in developing and maintaining your privacy program
Full-RAG: A modern architecture for hyper-personalizationZilliz
Mike Del Balso, CEO & Co-Founder at Tecton, presents "Full RAG," a novel approach to AI recommendation systems, aiming to push beyond the limitations of traditional models through a deep integration of contextual insights and real-time data, leveraging the Retrieval-Augmented Generation architecture. This talk will outline Full RAG's potential to significantly enhance personalization, address engineering challenges such as data management and model training, and introduce data enrichment with reranking as a key solution. Attendees will gain crucial insights into the importance of hyperpersonalization in AI, the capabilities of Full RAG for advanced personalization, and strategies for managing complex data integrations for deploying cutting-edge AI solutions.