MAB Training session

1. FruitBreedomics – Technical Session
Pyramiding scab and mildew resistance in
the Agroscope breeding program
I. Baumgartner (Agroscope)
San Giuseppe di Comacchio (Italy),
June 19th 2015

2. Overview
• Objective: pyramiding resistance genes
 two or more genes against the same pathogen
• Crosses: parent selection (traits? pedigree?)
• SNP markers
• Polymorphism in parents
• Interpretaion of results: demonstration in Excel

3. Mother (Resistances) Father (Resistances) Flowers Fruits Seeds
ACW 11303
(Rvi6=Vf)
ACW 18522
(Rvi6=Vf, Rvi2=Vh2)
2246 352 2227
ACW 13652
(Rvi6=Vf, Pl2)
ACW 11567
(Rvi2=Vh2)
1370 323 2793
Progeny 1
Progeny 2
Pilot studies crosses at Agroscope

4. • Phenotypic symptoms (single resistance genes)?
• Pedigree?
Be sure about the resistances

5. LG1
Rvi6
resistance
to apple
scab
Scab and mildew resistances located on different chromosomes
LG2 LG3 LG4 LG5 LG6 LG7 LG8
LG9 LG10 LG11 LG12 LG13 LG14
LG15
LG16 LG17
Rvi2
resistance
to apple
scab
Pl2
resistance
to powdery
mildew
Apple genetic linkage map (17 chromosomes)

6. Trait / Locus Marker
Scab resistance Rvi6=Vf (LG1) Rvi6_42M10SP6_Y124 (SNP)
Scab resistance Rvi2=Vh2 (LG2) Rvi2_region53_M417 (SNP)
Mildew resistance Pl2 (LG11) Pl2_3_Y211 (SNP)
Fruit texture Md-PG1 (LG10) PG_FEM_LC_19 (SNP)
Shelf life Md-ACO1 (LG10) ACO_FEM_cg_4 (SNP)
Shelf life Md-ACS1 (LG15) ACS_FEM_cg_5 (SNP)
Crispness (LG16) Crispness_SNP1 (ss475881704; SNP1 from RosBREED)
Acidity (LG16) Acidity_SNP2 (ss475876558; SNP2 from RosBREED)
Markers used

7. Literature on SNPs for scab and mildew resistance

8. Code SNP
M A/C
R A/G
W A/T
S C/G
Y C/T
K G/T
H A/C/T
V A/C/G
D A/G/T
B C/G/T
Jänsch et al. 2015

9. Polymorphism in parents
Cross 1206
Rvi6_42M10SP6_Y124 Rvi2_region53_M417 Pl2_3_Y211
ACW13652 CT CC CT
ACW11567 CC CA CC
T = Rvi6 resisance A = Rvi2 resistance T = Pl2 resistance
Cross 1205
Rvi6_42M10SP6_Y124 Rvi2_region53_M417
ACW11303 CT CC
ACW18522 CT CA
T = Vf resisance A = Rvi2 resistance

10. Demonstration in Excel

11. Thank you for your attention
powdery mildew
Agroscope good food, healthy environment