The document is a presentation on microscope maintenance and care, discussing its basic parts, components, and the appropriate practices for keeping it in good working condition. It emphasizes dos and don’ts, outlining the procedures for cleaning, storage, and regular maintenance checks. Additionally, it includes a maintenance chart for tracking the upkeep of the microscope.

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DODOMA REGIONAL REFERRAL HOSPITAL
SINGIDA COLLEGE OF HEALTH SCIENCE AND
TECHNOLOGY FIELD STUDENTS PRESENTATION
TOPIC: MICROSCOPE

2. Presentation outline
 Basic parts of the microscope
 Components of the microscope
 Identify materials used in correct care and
maintenance of the microscope;
 Maintenance and repair of microscope.
 The do‘s and don’ts’ when caring of a microscope
 Tool used for record keeping of maintenance of
microscope.
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3. THE PRIMARY TOOL : THE MICROSCOPE
Compound Microscope Teaching Microscope fitted with a Monitor Teaching microscope for Multiple Observers
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4. PRIMARY TOOL :THE MICROSCOPE
Teaching microscope for Multiple Observers
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5. COMPONENTS OF THE MICROSCOPE
Support
System
Magnification
System
Illumination
System
Adjustment
System
It consists of the base, arm, and stage. The base and
arm are structural elements which hold the other parts of
the microscope in place while the stage holds the slide
It is an optical compound microscope: Eyepiece
Lenses and Objective Lenses:
It consists of a light source, such as an electric
lamp or a light-emitting diode, and a lens system
forming the condenser.
It is the focusing parts which consists of two
knobs - the coarse adjustment knob and the fine
adjustment knob.
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6. COMPONENTS OF THE MICROSCOPE
Support System
Base: Must rest on a firm, flat bench or table
Supports the main tube and the mechanical
stage
Arm:
Holds the slide secure and allows the specimen
moved smoothly backwards,forwards and
sideways
Mechanical
Stage:
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7. COMPONENTS OF THE MICROSCOPE
Magnification System
Holds the objective lenses of different
magnification
Revolving
Nosepiece:
x4,x10,x40,x100 for magnification of the image
Objectives:
x10 for magnification of image
Eyepiece:
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8. COMPONENTS OF THE MICROSCOPE
Illumination
System
a)Main Tube
b)Body Tube (Prism)
c)Sub-stage Condenser
 Made up of number of lenses
 Centers the light from the mirror or
electric light source to central spot
on microscopic field
 Can be raised or lowered to
give maximum/minimum illumination
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9. COMPONENTS OF THE MICROSCOPE
Illumination
System
a)Iris diaphragm – controls the
amount of light passing through the
condenser
b)Light source – use to direct light
from light source to the microscopic
field
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10. COMPONENTS OF THE MICROSCOPE
Adjustment System
Used to focus the specimen being
examined
Coarse
Adjustment:
Used for finer focusing
Fine
Adjustment:
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11. Materials for care and Cleaning:
 Soft cotton cloth and lens cleansing tissue
 Special tissue paper or whole absorbent paper
 70% Alcohol
 Make-up brush (softest) /camel hairbrush
 soft cloth
 Plastic cover
CARE AND MAINTENANCE
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12. CARE AND MAINTENANCE
The eyepieces:
 Clean upper lens with a lens cleaning tissue
 Clean lower lens with a fine brush
The condenser
 Clean the same way as the objectives using a
soft cloth
The support and stage
 Clean using soft brush
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13. CARE AND MAINTENANCE:
(STORAGE OF A MICROSCOPE)
Laboratories with electricity:
 A warm cupboard heated by 1 or 2 light bulbs (40W)
Laboratories without electricity:
 A desiccator 15-20 cm in diameter with no less
than 250 g of dry blue silica gel (which indicates
humidity by turning pink)
** Important: The microscopes must be kept in the warm
cupboard even if the laboratory is air conditioned
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14. KEY MESSAGES
DOS
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• Line up the x10 objective (or empty socket) with the ocular when microscope is not in use
• Lower the stage and the condenser
• Adjust with x10 before moving x100 lens to slide
• Oil immersion objective must be cleaned after use
• Report to the Maintenance Department if any malfunction is observed
• Cover the microscope when not in use

15. KEY MESSAGES
DON’TS
• Clean the lenses of the objectives and eyepieces with xylene
• Use ordinary paper or cotton wool to clean the lenses
• Touch the objectives with your finger
•
• Clean the inside lenses of the objectives and eyepieces with cloth or paper
• Keep the microscope in a closed wooden box in hot humid places
• Carry the microscope by the limb with one hand
• Expose the microscope to direct sunlight
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16. MICROSCOPE MAINTENANCE CHART
MICROSCOPE
SERIAL # MONTH 20
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QA Review
Date:
Audit .
DATE OF MONTH
DAILY MAINTENANCE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Check all optics for damage
Check coarse/ fine adjustments
Dust/clean surfaces of scope components
Clean externalsurface
Remove oil
Replace bulbs as needed
Microscopecoveredafter use
TECH’S INITIALS
WRAIR PB#
COMMENTS
1)………………………………………………………………………………………………………………………………………………………………………..
2)………………………………………………………………………………………………………………………………………………………………………….
3)………………………………………………………………………………………………………………………………………………………………………….
QA Check

17. ANKS FOR YOUR TIME