This document discusses applications of NMR, MRI, and micro-MRI techniques. It summarizes that these techniques can be used to image lipids, liposomes, plant tissues, biofilms, tumor spheroids, Xenopus laevis cells, and composite materials at the microscale level. Specific applications mentioned include online observation of drug penetration and destruction of tumors, monitoring seed protection and germination, and observing the effects of agents on tumor spheroids over time. The document also discusses potential future directions for miniaturization and combining MRI with other complementary methods.

1. ”Arbeitsgruppe NMR - MRI- MR-MicroImaging“
Molecule (Lipid)
nm
selforganization
Liposomes
50-500nm
Biosensor3D-MR-Micro-
Imaging of a survival
plant (submillimeter)
Technology
Application

2. •Life and Materials Sciences
•Drug Encapsulation
•Online Observation of Destruction of Tumors
•Seeds: Protection, Germination
•Composite Materials
•Drug penetration through the skin (online observation)
........
A
PD Dr. Frank Volke Magnetic Resonance

3. 3He / 1H lung coil for
Siemens Scanner 1,5 T
Technical Solutions (e.g.)
PD Dr. Frank Volke Magnetic Resonance

4. MRI-Endoskop:
NMR-Spektroskopie und MRI
PD Dr. Frank Volke Magnetic Resonance
• MRI-Endoskop (endoluminal)
Cooperation: Univ.-
Heidelberg/MR Inside
• Foldable coil, stabilization,
positioning via optical channel,
works with Siemens 1,5 T
clinical Scanner. Very good
homogeneity and resolution
(compare MRI Image and
microscope Image)
2 mm

5. NMR-MRI-µMRI
Biofilms (flow, structure, forces)
B. Manz, F. Volke, D. Goll, H. Horn, Measuring Local Flow Velocities
and Biofilm Structure in Biofilm Systems with Magnetic Resonance
Imaging (MRI),
Biotechnology and Bioengineering 84, 424-432 (2003).

6. 13C-1H correlation spectrum
(MAS at 8000 Hz) of a pure
POPC /water dispersion (2H2O)
at 310 K
Volke, HRMAS in Biomembrane Research
BRUKER REPORT 145: ISSN 0724-0185
NOESY 1H

7. NMR-MRI-µMRI
Xenopus laevis
• Single cell imaging at 600 MHz Proton-NMR (14,1 T)
• The effect of strong magnetic fields on cells
Daniel Mietchen @
PD Dr. Frank Volke Magnetic Resonance

8. Rim
with
Quiecent
Cells
Necrotic
zone
Nutrient
Solution
Proliferating Cells
MC Cell
Multicellular
Tumor Spheroid
NMR Micro Imaging (on-line). Effect of Agents on Tumor Spheroids
On-line monitoring of drug
effects during several hours
Imaging of structural and
motional properties
Diagnostic tool
Dr. Frank Volke; IBMT,
Cells
MCTS
In vivo
Micro imaging
Calculated Diffusion Images
0,0 mm 3,0 mm
0,0 mm
3,0 mm
11 h nach HEMAD[10-9 m²s-2 ]
0
4
0,0 mm 3,0 mm
0,0 mm
3,0 mm
16 h nach HEMAD[10-9 m²s-2 ]
0
4
Jörg Breidt
vor HEMA
PD Dr. Frank Volke Magnetic Resonance

9. NMR-MRI-µMRI
Quo vadis ?
•Miniaturization !!!
•Combination of MR with Complementary Methods
(US,Laser, IR, OCT)
•Micro-flow Imaging
•Lab-on-chip (Micro-coils)
•Broad range of composite materials, incl. organic ones: e.g.
•Cryobiology, Hydration
•Biofilms, Surface characterization
•Drug-encapsulation
PD Dr. Frank Volke Magnetic Resonance