Osteosarcoma is the most common type of bone cancer that occurs most often in children and adolescents. This study aims to reveal the mechanism of how miR-139 regulates osteosarcoma progression by targeting ITGAV. The expression of miR-139 and ITGAV were detected in osteosarcoma tissue samples using qRT-PCR and western blot. The effects of miR-139 and ITGAV on cell cycle were measured using flow cytometry. The results demonstrate that miR-139 inhibits osteosarcoma progression by targeting ITGAV.

1. Valentina Troitiño, III semester. Universidad Pontificia Bolivariana

2. Osteosarcoma
❏ Is the most common type of bone cancer, commonly happens
in the growing part of long bones such as the femoral
metaphysis.
❏ It happens most often in children, adolescents, and young
adults. Osteosarcoma have more prevalence in males than in
females.
❏ Osteosarcoma may grow into nearby tissues, such as tendons
or muscles. It may also spread, or metastasize, through the
bloodstream to other organs or bones in the body.

3. Introduction
❏ MiR-139: A non-coding RNA that's involved in
post-transcriptional regulation of gene expression.
It's believed that mir-139 could inhibit the biological behavior
of osteosarcoma cells and the tumor growth via targeting
ITGAV.
❏ MG63: Is one of the cell lines commonly used for research
studies of Osteosarcoma.

4. Objective
Osteosarcoma is a relatively common primary malignant bone
tumor in clinic, which frequently occurs in children and
adolescents. It is essential to clarify the molecular mechanism of
osteosarcoma to provide better diagnosis and treatment.
Abnormal expression of miRNAs is closely related to the
pathogenesis and progression of osteosarcoma. MiRNAs play a
regulatory role in tumorigenesis and development of
osteosarcoma. The purpose of this study is to reveal the working
mechanism of miR-139/ITGAV axis in osteosarcoma progression.

5. Methods
❏ qRT-PCR: The reverse transcription of RNA to complementary
DNA. Was used to detect the expression of miR-139 and ITGAV
in osteosarcoma tissue.
❏ Western blot: Antigens are distributed to determine if they
are reacting with the patient’s antibodies using a
polyacrylamide gel electrophoresis. Was used to indicate the
amount of the protein expression of ITGAV in osteosarcoma
tissue.

6. Methods
❏ Immunohistochemistry: The marked antibody interacts with
the antigen to show up a specific cell. Was used to indicate the
amount of the protein expression of ITGAV in osteosarcoma
tissue.
❏ Flow cytometry: It is based on having one or more lasers
applied to a non turbulent jet of a solution in which the cells
circulate at high speed. It was used to measure the effects of
miR-139 and ITGAV on cell cycle of MG63.

7. Results
Fig. 1 C
Fig. 1 G

8. Results
Fig. 2 B-D Fig. 3 C

9. Discussion
Author Statement
Does the statement fulfill
with the article?
Xu et al., 2014;
Wang et al.,
2019a,b,c).
Recent studies have shown that miRNAs play a role in the
progression of osteosarcoma.
Liu et al.,
2016; Huang et
al., 2016.
miRNA wields a crucial role by targeting and regulating its
upstream and downstream genes, forming a complex
regulatory network.
(Hongdan and
Feng, 2018;
Huang et al.,
2018).
After binding to the specific sequence of the target gene
3’-UTR, miRNA leads to the degradation or translation
inhibition of mRNA, and negatively regulates the expression
of the target gene, thus exerting its biological function.

10. Conclusions
❏ Based on the knowledge in molecular biology and the
methods used in this article we can find therapeutic
targets to know who should be attacked when treating
osteosarcoma, supported by the new “personalized
medicine”
❏ The research done with miRNA helps to find new
biomarkers for diagnosis or follow-up of patients with
osteosarcoma due to the increase in the gen ITGAV.