2. Colorectal cancer is a desense that develops in
the colon or rectum, two important parts of the
digestive system.
Cause
It is caused by polyps (beligns) in the lining of the
colon or rectum that over time become cancer
Colorectal cancer
3. Metastasis
A metastasis is the spread of cancer cells
from the original tumor to other parts of
the body. It is the process by which
cancer is sead to organs or tissues other
than where it originated.
4. Evaluate the regulation of tumor metastasis and CD8
+ T cell infiltration by the circRNF216/miR-576-
5p/ZC3H12C axis in colorectal cancer
5. Methods
WB assay and antibodies
In this study, colorectal cancer
was being evaluated and the
influence of these proteins on the
metastasis process, for which the
western blot was done.
Cuantitative real-time PCR (qrt-
PCR)
The PCR test is used to amplify the
nucleic acid of interest, in this
case a circRNA, miRNA, mRNA
6. Methods
Luciferase reporter assay
It is a laboratory technique used to
measure the function of luciferase,
it is an enzyme that produces light
when oxidized. The amount of light
produced is proportional to the
amount of luciferase present in the
sample
Immunohistochemistry
Immunohistochemistry (IHC) is a
laboratory technique used to detect
the presence of specific proteins in
cells or tissues. In the trial it was
used for ZC3H12C and N-cadherin in
lung tissue
9. Author
what did the author
say?
agree or disagree?
Panda AC. Circular RNAs Act as miRNA
sponges.
Tousands of circRNAs
have been identifed in eukaryotes thanks to
the advances in high-throughput sequencing
and unbiased computational approaches
AGREE
Sharma P, Shen Y, Wen S, Yamada S,
Jungbluth AA, Gnjatic S, et al. CD8
tumor-infltrating lymphocytes are
predictive of
survival in muscle-invasive urothelial
carcinoma
Of note, intratumoral CD8+ T cells in TIME
are a critical positive predictor of clinical
outcomes
AGREE
Chen YG, Kim MV, Chen X, Batista PJ,
Aoyama S, Wilusz JE, et al. Sensing
Self and Foreign Circular RNAs by
Intron Identity.
Mol Cell.
CircRNAs leads to innate immunity gene
expression that presents pro?tection against
viral infection
AGREE
Discussion
10. Conclusion
• Molecular biology allows diseases to
be identified and characterized
more precisely at the molecular
level.
• Molecular biology provides
fundamental information about the
molecular mechanisms of diseases.