Seminario Biologia Molecular. Tercer semestre de Medicina Universidad Pontificia Bolivariana. Cancer de pulmon con metastasis en hueso

1. Hector Daniel Grisales Daza
3rd Semester
Faculty of Medicine
Pontifical Bolivarian University

2. Why it happens?
Introduction
Annual incidence is greater than
1,600,000 cases.
More than 1,370,000 people die each
year from this disease
It is the most common malignant tumor in
men worldwide. In women, it ranks fourth
in incidence. Lung cancer is also the cancer
with the highest mortality rate regardless
of gender.
It occurs when abnormal cells grow
out of control in the lungs, forming
tumors. These tumors can interfere
with the normal functioning of the
lung and, in advanced stages, can
spread to other parts of the body
through the lymphatic system or
bloodstream, in a process known as
metastasis.
Cancer of lung
Epidemiology
Non-small cell lung cancer, most
common (about 85% of cases)
Small cell lung cancer: less common
(about 15% of cases), more dangerous
Types
Causes
Smoking (main risk factor), 90% of cases
are due to this
Exposure to wood smoke
Exposure to metals (nickel, arsenic,
radon and cadmium)

3. Micro Rna- 182/interleukin-8 regulatory axis in
osteolytic bone metastasis of lung cancer
Introduction
Micro Rna 182 (Mir 182) plays a crucial role in the osteoclastic regulation of lung cancer metastasis in NSCLC.
Mir 182 interferes with the IL8 regulation pathway, called the KLHL21:NF-kB, specifically
acting by inhibiting the production of an inhibitor of IL 8 synthesis, this leads to
an increase in the expression of IL8 and others pro factors tumors.
As a result, the phosphorylation of the STAT3 and NFATc1 pathway present in pre-osteoclastic cells is stimulated,
which in turn accelerates the cellular differentiation of osteoclasts in the bone marrow.

4. To evaluate a novel microRNA-
182/interleukin-8 regulatory axis
that controls osteolytic bone
metastasis of lung cancer
General objetive
Objetive

5. PCRq Secuenciación
Métodos
Es una técnica que permite amplificar y
cuantificar en tiempo real una pequeña
cantidad de ADN o ARN, en una muestra hasta
obtener cantidades significativas para su
análisis. Esto se logra mediante ciclos
repetitivos de calentamiento y enfriamiento.
Se usó en el artículo para evaluar el grado de
expresión de algunos ARNs que participan en
la metástasis del cáncer de pulmón
Es el proceso de determinar el orden
específico de las bases nitrogenadas
(adenina, timina, uracilo citosina y
guanina) en una región específica de una
molécula de ácido nucleico, ya sea ADN o
ARN, se realizó para establecer la relación
entre el mir 182 y el KLHL21

6. Métodos
Inmunoblot Elisa
Es una prueba que se clasifica como un
inmunoensayo el cual se hace una electroforesis para
separar las proteínas y posteriormente se
identifican proteínas específicas por medio de una
reacción con anticuerpo, se usó para evaluar la
presencia del biomarcador (mir 182)
en las pruebas de cáncer de pulmón
Es una prueba de inmunoensayo la cual se
basa en la interacción específica entre un
anticuerpo y su antígeno, este último
enlazado a una enzima, la cual mediante
algún producto detectable, como, por ejemplo,
el cambio de color, hará posible la medición
del antígeno en la muestra utilizando la
espectrofotometría. Se usó para determinar
niveles cuantitativos de diferentes proteinas
como IL-8, IL-1, IL-12, and CXCL3

7. Resultados
Control

8. Resultados

9. AUTHORS What did they say? AGREE OR NOT
Discussion
"Multiple studies have shown that IL-8 is also overexpressed
in a variety of cancer cell lines and functions as an autocrine
and/or paracrine growth factor to facilitate tumor growth,
invasion, metastasis, and angiogenesis"
"MiR-182 is highly expressed in sensory tissues and organs,
and its function is involved in the inner ear and retina
development, as well as osteoclast and T cell differentiation"
"Also, both bone- metastatic myeloma and breast cancer cells
are able to stimulate the production of IL-6 by bone marrow
stromal cells, resulting in increased osteoclast differentiation
and bone destruction "
Krishnan V, Shuman LA,
Sosnoski DM, et al.
Ogawa R, Yamamoto
T, Hirai H, Hanada K,
et al.
Wei Q, Lei R, Hu G.

10. Thanks to molecular biology, in this articule has could shown several
advances for the cancer of lungs
Conclusion #1
By recognizing the mir 182/IL-8/STAT3 axis, effective treatments
can be proposed that interrupt certain segments of the
osteoclastogenic pathway
Conclusion #2
Identify cytokines/molecules that suggest a pulmonary metastatic
process to bone (IL-8, IL-1, IL-12, and CXCL3 P.e), resulting in a
more accurate and quick diagnosis
Conclusions

11. Mapa conceptual