Metal Ion Lab Introduction
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This lab experiment aims to separate iron(II), nickel(II), and copper(II) species using ion exchange chromatography. Ion exchange chromatography relies on charge interactions between ions in the sample and charges on the resin stationary phase. A strong anion exchange resin will be used to separate the species, and visible spectroscopy will then qualitatively determine concentrations and evaluate the separation effectiveness. The experiment allows analysis of species' differing affinities for the resin and responses to the mobile phase composition.
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Ion Exchange Chromatography Lecture
Ion-exchange chromatography separates molecules on the basis of charge. The stationary phase contains resin beads with cationic or anionic functional groups that can bind positively or negatively charged molecules. Proteins either bind to the resin based on their net charge or pass through. Elution is achieved by changing pH or adding salts to compete for binding sites on the resin. Modern systems automate ion-exchange chromatography using gradients controlled by multiple pumps and fraction collectors.
Chiral chromatography & ion pair chromatography
This document discusses chiral chromatography and ion-pair chromatography. It begins with definitions of stereoisomers including enantiomers, diastereomers, and racemates. It then covers the principles of chiral chromatography, which involves using a chiral stationary phase or chiral derivatization to separate enantiomers. Applications include resolving drug enantiomers. The document also discusses ion-pair chromatography, which uses ion-pairing reagents to separate charged analytes in HPLC. Key factors that affect ion-pair chromatography are also reviewed.
Affinity chromatography 1
Ion exchange chromatography separates components based on their surface charge by using a stationary phase with oppositely charged functional groups. The document provides background on the history and development of ion exchange and other chromatography techniques. It explains the principles and applications of ion exchange chromatography, including how it uses resins and gradients to differentially elute ions based on their affinity for the stationary phase.
Ion exchange chromatography
Theoretical background
Cont’d
Ion exchangers
There are three classes of ion exchangers , these include
Resins
Gels
Inorganic exchangers
Selectivity for ion exchange
In general , ion exchangers favour the binding of ions of
Higher charge
Decreased hydrated radius
Increased polarizability
Ion exchange resins are used for the separation of small molecules.
Ion exchange gels are used for the separation of large molecules like protiens ,nucleic acids.
Separations involving harsh chemical conditions(high temperature , high radiation levels, strongly basic solutions or powerful oxidizing agents) employ inorganic ion exchangers
Advantages
Detectability: useful for the detection of many in-organic salts and organic ions with poor uv absorptivity like alkyl amines or sulfonates.
Preparative separations: usually preferred because of the availability of volatile buffers . volatile buffers makes the removal of mobile phase easier.
Useful to resolve very complex samples, i.e in the case of multi step separation
Useful for separation of mixtures of biological origin, in organic salts and some organo- metallics
Applications
Conversion from one salt to other e.g we can prepare tetra propyl ammonium hydroxide from a tetra propyl salt of some other anion.
household (laundry detergents and water filters) to produce soft water
Ion exchange is used to prepare de-ionized water
separate and purify metals
Dealkalization
analysis and purification of immunoglobulins
Separation of inorganic ions
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This document discusses spectrophotometry techniques for measuring light absorption by molecules. It covers the electromagnetic spectrum, Beer-Lambert law, applications of UV-vis spectroscopy like determining cell density and protein concentration, and methods for measuring absorbance of molecules like DNA, RNA, proteins, and other biological compounds. Key concepts explained include the relationship between absorbance, molar extinction coefficient, concentration, and path length in the Beer-Lambert law.
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The document discusses the effect of counter-ion type and concentration on the retention of various compounds in liquid chromatography. Specifically, it examines how the retention of β-blockers, alkaloids, and oligolysines is affected by changing the concentration of counter-ions like perchlorate, heptafluorobutyrate, and trifluoroacetate in the mobile phase. The retention was found to increase with rising counter-ion concentration. Different counter-ions also resulted in varying retention, with some ions producing stronger interactions than others. Peak efficiency and symmetry were also influenced by the mobile phase counter-ion concentration.
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Recommended
Ion Exchange Chromatography Lecture
Ion-exchange chromatography separates molecules on the basis of charge. The stationary phase contains resin beads with cationic or anionic functional groups that can bind positively or negatively charged molecules. Proteins either bind to the resin based on their net charge or pass through. Elution is achieved by changing pH or adding salts to compete for binding sites on the resin. Modern systems automate ion-exchange chromatography using gradients controlled by multiple pumps and fraction collectors.
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Ion exchange chromatography separates components based on their surface charge by using a stationary phase with oppositely charged functional groups. The document provides background on the history and development of ion exchange and other chromatography techniques. It explains the principles and applications of ion exchange chromatography, including how it uses resins and gradients to differentially elute ions based on their affinity for the stationary phase.
Ion exchange chromatography
Theoretical background
Cont’d
Ion exchangers
There are three classes of ion exchangers , these include
Resins
Gels
Inorganic exchangers
Selectivity for ion exchange
In general , ion exchangers favour the binding of ions of
Higher charge
Decreased hydrated radius
Increased polarizability
Ion exchange resins are used for the separation of small molecules.
Ion exchange gels are used for the separation of large molecules like protiens ,nucleic acids.
Separations involving harsh chemical conditions(high temperature , high radiation levels, strongly basic solutions or powerful oxidizing agents) employ inorganic ion exchangers
Advantages
Detectability: useful for the detection of many in-organic salts and organic ions with poor uv absorptivity like alkyl amines or sulfonates.
Preparative separations: usually preferred because of the availability of volatile buffers . volatile buffers makes the removal of mobile phase easier.
Useful to resolve very complex samples, i.e in the case of multi step separation
Useful for separation of mixtures of biological origin, in organic salts and some organo- metallics
Applications
Conversion from one salt to other e.g we can prepare tetra propyl ammonium hydroxide from a tetra propyl salt of some other anion.
household (laundry detergents and water filters) to produce soft water
Ion exchange is used to prepare de-ionized water
separate and purify metals
Dealkalization
analysis and purification of immunoglobulins
Separation of inorganic ions
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This document discusses spectrophotometry techniques for measuring light absorption by molecules. It covers the electromagnetic spectrum, Beer-Lambert law, applications of UV-vis spectroscopy like determining cell density and protein concentration, and methods for measuring absorbance of molecules like DNA, RNA, proteins, and other biological compounds. Key concepts explained include the relationship between absorbance, molar extinction coefficient, concentration, and path length in the Beer-Lambert law.
Ion par chromatography
The document discusses the effect of counter-ion type and concentration on the retention of various compounds in liquid chromatography. Specifically, it examines how the retention of β-blockers, alkaloids, and oligolysines is affected by changing the concentration of counter-ions like perchlorate, heptafluorobutyrate, and trifluoroacetate in the mobile phase. The retention was found to increase with rising counter-ion concentration. Different counter-ions also resulted in varying retention, with some ions producing stronger interactions than others. Peak efficiency and symmetry were also influenced by the mobile phase counter-ion concentration.
Jastine powerpoint (chromatography)
Chromatography separates mixtures by exploiting differences in how compounds interact with stationary and mobile phases. There are several types including adsorption chromatography which uses a solid stationary phase that adsorbs compounds from a liquid or gas mobile phase. Partition chromatography employs a liquid stationary phase coated on a solid support that compounds partition between. Affinity chromatography most selectively separates compounds based on specific interactions like antibody-protein binding.
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This document provides an overview of ion exchange chromatography. It describes the basic principles and types, including cation exchange which attracts positively charged molecules to a negatively charged resin, and anion exchange which attracts negatively charged molecules to a positively charged resin. Common resins for each type are described. The document outlines the basic process, including equilibrium, sample application, elution, and regeneration. Factors that affect ion exchange chromatography like resin properties, pH, mobile phase modifiers are also summarized. Finally, some applications of ion exchange chromatography are listed such as water softening and purification, and separation of protein and inorganic mixtures.
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This document provides an overview of ion exchange chromatography. It describes the basic principles of how ion exchange chromatography separates ions and polar molecules based on their charge. The document outlines the different types of ion exchangers used as stationary phases in cation exchange chromatography and anion exchange chromatography. It also discusses several factors that can affect the separation process and provides some examples of applications for ion exchange chromatography.
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Ion exchange chromatography
This document discusses ion exchange chromatography, including its principle, types of ion exchange resins, practical requirements, factors affecting separation, and applications. Ion exchange chromatography separates similar charged ions through reversible ion exchange between ions in solution and ions on an ion exchange resin. There are two main types of resins: cation exchange resins that separate cations, and anion exchange resins that separate anions. Key factors that affect ion exchange separation include the nature and properties of the resin, and the nature of the exchanging ions. Ion exchange chromatography has applications in water softening, producing deionized water, separating and purifying metals and ions, and analysis and purification of biomolecules.
ion exchange chromatography
Ion exchange chromatography is a process that separates ions based on their charge. It works by reversibly exchanging ions in solution with ions bound to an inert resin. There are two main types of ion exchange resins: cation exchangers that bind positively charged ions and anion exchangers that bind negatively charged ions. Samples containing mixtures of ions are passed through a column packed with the resin, and ions of opposite charge bind to the resin while others pass through. The bound ions can then be selectively eluted by altering conditions like increasing salt concentration or pH. Ion exchange chromatography has applications in separating amino acids, nucleic acids, metals, vitamins and other biological molecules.
Ion exchange chromatography
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chromatographic methods (that is a mobile phase is a liquid and a stationary phase is a
solid).
ION CHROMATOGRAPHY ION CHROMATOGRAPHY
ION CHROMATOGRAPHY , THE CHROMATOGRAPHY BASED ON ITS TYPES LIKE RESINS AND MORE ITS TYPES OFION EXCHANGE RESINS, THE DIFFERENT TYPES HAVE DIFFERENT ADVANTAGES AND LIMITATIONS
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2. The simulation results from the developed models showed good agreement with reported experimental data, with an average percentage absolute error of 8.95%.
3. Key findings were that the hollow fiber supported liquid membrane system using a synergistic mixture of D2EHPA and TOPO extractants is effective for the extraction of neodymium ions from solution.
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Ion Exchange Chromatography
Ion exchange chromatography separates ions and polar molecules based on their affinity for an ion exchanger. There are two main types: cation exchange attracts positively charged molecules to a negatively charged resin, while anion exchange attracts negatively charged molecules to a positively charged resin. The stationary phase is an ion exchange resin and the mobile phase is usually a liquid or gas that carries sample components through the resin. The process involves equilibrating the resin, adsorbing the sample so charged molecules bind to the resin, then eluting bound molecules by increasing ionic strength so they detach from the resin in a purified form.
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Ion exchange chromatography
Ion exchange chromatography is a technique that separates ions and polar molecules based on their charge. It works by using an ion exchange resin with charged functional groups that interact with and retain analyte ions of the opposite charge from a mobile phase. Common stationary phases use functional groups like sulfonate, carboxylate or quaternary amine. The document discusses the history of ion exchange chromatography and provides examples of its applications including protein purification, water analysis, separation of amino acids, vitamins and drugs. Factors that affect separations like pH, ionic strength, temperature and mobile phase modifiers are also summarized.
Ion exchange chromatography
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organic pollutants from large volumes of water. This article reviews the practical feasibility of various kinds of raw
and surface modified carbon nanotubes for adsorption of heavy metal ions from wastewater. Further, properties of
CNTs (adsorption sites), characterization of CNTs (pore volume, BET surface area, surface total acidity, surface
total basicity) and solution properties (ionic strength, effect of pH) are explained very well. The adsorption
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an assignment on ion exchange chromatography
Ion exchange chromatography is a separation technique based on charge interactions between molecules and an ion exchange resin. There are two types of resins - cation exchangers that attract negatively charged molecules and anion exchangers that attract positively charged molecules. The separation is achieved by altering the mobile phase buffer pH and salt concentration to selectively elute molecules from the stationary phase resin based on their charge. Ion exchange chromatography is useful for purifying and separating a wide range of biomolecules like proteins, nucleotides, and amino acids while preserving their structure.
ion exchange chromatography
This document provides an overview of ion exchange chromatography. It describes the basic principles of how ion exchange chromatography separates ions and polar molecules based on their charge. The document outlines the different types of ion exchangers used as stationary phases in cation exchange chromatography and anion exchange chromatography. It also discusses several factors that can affect the separation process and provides some examples of applications for ion exchange chromatography.
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Ion exchange chromatography
This document discusses ion exchange chromatography, including its principle, types of ion exchange resins, practical requirements, factors affecting separation, and applications. Ion exchange chromatography separates similar charged ions through reversible ion exchange between ions in solution and ions on an ion exchange resin. There are two main types of resins: cation exchange resins that separate cations, and anion exchange resins that separate anions. Key factors that affect ion exchange separation include the nature and properties of the resin, and the nature of the exchanging ions. Ion exchange chromatography has applications in water softening, producing deionized water, separating and purifying metals and ions, and analysis and purification of biomolecules.
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Ion exchange chromatography is a process that separates ions based on their charge. It works by reversibly exchanging ions in solution with ions bound to an inert resin. There are two main types of ion exchange resins: cation exchangers that bind positively charged ions and anion exchangers that bind negatively charged ions. Samples containing mixtures of ions are passed through a column packed with the resin, and ions of opposite charge bind to the resin while others pass through. The bound ions can then be selectively eluted by altering conditions like increasing salt concentration or pH. Ion exchange chromatography has applications in separating amino acids, nucleic acids, metals, vitamins and other biological molecules.
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chromatographic methods (that is a mobile phase is a liquid and a stationary phase is a
solid).
ION CHROMATOGRAPHY ION CHROMATOGRAPHY
ION CHROMATOGRAPHY , THE CHROMATOGRAPHY BASED ON ITS TYPES LIKE RESINS AND MORE ITS TYPES OFION EXCHANGE RESINS, THE DIFFERENT TYPES HAVE DIFFERENT ADVANTAGES AND LIMITATIONS
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1. A mathematical model was developed to simulate the synergistic extraction of neodymium ions using a hollow fiber supported liquid membrane with a mixture of 0.5M D2EHPA and 0.5M TOPO extractants.
2. The simulation results from the developed models showed good agreement with reported experimental data, with an average percentage absolute error of 8.95%.
3. Key findings were that the hollow fiber supported liquid membrane system using a synergistic mixture of D2EHPA and TOPO extractants is effective for the extraction of neodymium ions from solution.
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Gaas' hplc
This document provides information on chromatography techniques. It discusses the basic components and processes involved in chromatography. The key techniques described are column chromatography, planar chromatography, gas chromatography, liquid chromatography, and affinity chromatography. It also summarizes different modes of chromatography such as normal phase chromatography, reverse phase chromatography, ion exchange chromatography, size exclusion chromatography, and affinity chromatography.
Ion Exchange Chromatography
Ion exchange chromatography separates ions and polar molecules based on their affinity for an ion exchanger. There are two main types: cation exchange attracts positively charged molecules to a negatively charged resin, while anion exchange attracts negatively charged molecules to a positively charged resin. The stationary phase is an ion exchange resin and the mobile phase is usually a liquid or gas that carries sample components through the resin. The process involves equilibrating the resin, adsorbing the sample so charged molecules bind to the resin, then eluting bound molecules by increasing ionic strength so they detach from the resin in a purified form.
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Ion exchange chromatography
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Ion exchange chromatography
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The document discusses ion exchange chromatography, which separates charged molecules by exchanging them for ions attached to an insoluble matrix. It describes the principle of reversible ion exchange between oppositely charged molecules and the matrix. The document outlines the types of ion exchange resins used, including polystyrene and cellulose. It also discusses cation and anion exchangers, preparation of ion exchangers, factors affecting separation, and applications such as water softening and analyzing nucleic acids.
Ion exchange chromatography
Ion exchange chromatography uses charged sites on a stationary phase to selectively retain ionized solutes from a mobile phase based on electrostatic attraction. Cation exchangers contain negatively charged groups that attract positively charged cations, while anion exchangers contain positively charged groups that attract negatively charged anions. Key factors that influence selectivity include ion charge, hydrated radius, and polarizability. Ion exchange chromatography has various applications including separation of ions, removal of interferents, water softening, and demineralization.
Ion Exchange Chromatography, ppt
Ion exchange chromatography is a technique used to separate mixtures of similarly charged ions using an ion exchange resin. The resin works by reversibly exchanging ions between those present in the solution and the resin. There are different types of ion exchange resins classified by their chemical nature (strong/weak cation or anion exchangers) and source (natural or synthetic). Successful ion exchange requires the resin to be chemically stable, insoluble, sufficiently cross-linked, and contain exchange groups.
Water Quality Control and Treatment Water Treatment
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water treatment slides
1) Water treatment involves ensuring a safe and clean drinking water supply. It requires identifying a water source and protecting it from contamination through appropriate treatment and distribution.
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Ion exchange chromatography2
The document is a report on a seminar about ion exchange chromatography. It discusses the principles and mechanisms of ion exchange chromatography. Key points include:
- Ion exchange chromatography separates molecules based on differences in their net surface charge using anion or cation exchange resins with oppositely charged groups.
- Separation occurs as sample molecules reversibly adsorb to the ion exchanger via electrostatic interactions that can be controlled by modifying the pH or ionic strength of the mobile phase buffer.
- Different techniques like isocratic or gradient elution allow selective desorption and elution of sample components from the column.
- Proper choice of ion exchanger, buffer pH, and other parameters depends
analytical chemistry 2 ppt.pptx
Ion exchange chromatography uses an insoluble matrix with electrostatically bound ions that can reversibly exchange with ions in a solution. The technique separates charged compounds like proteins that differ by a single amino acid. It works by attracting oppositely charged analytes to a stationary phase made of an inert support coupled to positive or negative functional groups, which the analytes can exchange with to bind to the matrix and be separated from other compounds based on their charge.
Ion exchange chromatography
This document discusses ion exchange chromatography, including its principle, types of ion exchange resins, practical requirements, factors affecting separation, and applications. Ion exchange chromatography separates ions based on their affinity for ion exchange resins through reversible ion exchange reactions. There are two main types of resins - cation exchange resins that separate cations and anion exchange resins that separate anions. Key factors that affect ion exchange separations are the nature of the ions and properties of the resins, such as cross-linking and swelling. Ion exchange chromatography has various applications, including water softening, producing deionized water, separating and purifying metals and ions, and analysis/purification in fields like biochemistry.
Ion exchange chromatography
Ion exchange chromatography works under the principle of reversible adsorption and this method involves the separation of ions by using different types of exchange resins based on the ions to be separated.
Ion Chromatography Paper
Ion chromatography is a form of liquid chromatography that separates and quantifies ions and ionizable molecules. It utilizes different stationary phases and separation techniques including ion exchange, ion exclusion, ion pairing, and ion suppression chromatography. Ion exchange chromatography separates ions based on their affinity for oppositely charged groups on the stationary phase. Sample preparation techniques such as dialysis and solid phase extraction are used to reduce matrix effects. Ion chromatography is widely used in environmental analysis due to its ability to detect ions at trace levels. It has been used to detect perchlorate in drinking water within 7 minutes with a detection limit of 20 μg/L.
Ion exchange chromatography
The document discusses ion exchange chromatography, which separates charged compounds by reversible ion exchange between ions in solution and ions bound to an insoluble matrix. It works by binding either the compound of interest or contaminants to the column. The document covers the principle, working, types, requirements, and applications of ion exchange chromatography. It discusses cation and anion exchangers used as the stationary phase and factors that affect separation like pH, ionic strength, and temperature.
Ion excghnge chromatography
This document summarizes a seminar on ion exchange chromatography. It introduces the topic, covering the basic principles of how ion exchange chromatography separates ions and polar molecules using stationary phases with positively or negatively charged groups. It then discusses the types of ion exchange resins, including classifications based on chemical nature and source. Examples of applications are given, such as separating similar ions, water softening, demineralization, separating sugars and amino acids. The document concludes by discussing some advantages and disadvantages of ion exchange chromatography.
Ion-Exchange-Chromatography.pptx
Ion exchange chromatography separates ions and polar molecules based on their charge properties. It works by having a stationary phase with charged functional groups that attract and bind to oppositely charged molecules in the mobile phase. There are two main types - cation exchange chromatography which binds positively charged molecules, and anion exchange chromatography which binds negatively charged molecules. Proteins can be separated using ion exchange chromatography by adjusting the pH and ionic concentrations to manipulate the net charge on the proteins. Ion exchange chromatography has applications in separating molecules like amino acids, proteins, and nucleotides.
Ion exchange chromatography - by Shree
Download and play it my friends it contain VIDEO
The technique of ion exchange chromatography is based upon the interaction between charged solute molecules and oppositely charged moieties covalently linked to chromatographic matrix.
The reasons for its widespread success is its applicability, high resolving power, high capacity and simplicity of the technique.
Separation in ion exchange chromatography depends upon the reversible adsorption of charged solute molecules to immobilized ion exchange groups of opposite charge. Most experiments are performed by following : Video For Understanding Play It
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Chromatography is a technique used to separate the components of a mixture. There are several types of chromatography based on the mechanism of separation, including ion-exchange, affinity, size-exclusion, adsorption, and partition chromatography. The document provides definitions and principles of each type as well as examples of their applications in separating biochemical compounds like proteins, peptides, amino acids, and alkaloids. Chromatography is widely used in fields like biochemistry, forensic science, and environmental analysis.
Ion exchange chromatography -SlideShare
This ppt provide a good knowledge about ion exchange chromatography. I think this is very helpful for you .Here i have tried to explain a best way and simple method so guys you all enjoy this and gain your knowledge. And wish for me to provide more pptx for you all .at the end i want your experience give me suggestion if i made any mistake thank you .
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This document provides an overview of chromatography and mass spectroscopy. It explains that chromatography is a technique used to separate mixtures by partitioning components between a stationary and mobile phase. Mass spectroscopy determines the mass of molecules by ionizing samples and separating ions based on their mass-to-charge ratio, producing spectra that can identify compounds. The document describes various chromatography and mass spectroscopy techniques and processes, such as how they work, their components, and advantages of combining the two methods.
Ion Exchange Chromatography.pptx
Principle, classification, Properties, Advantages and Applications of Ion Exchange Chromatography are discussed
Ion Exchange Chromatography.pptx
Ion exchange chromatography is a process that separates ions and polar molecules based on their interactions with an inert matrix. There are two types of ion exchangers - cationic exchangers that attract positively charged cations, and anionic exchangers that attract negatively charged anions. The principle of separation relies on the reversible exchange of ions between the analyte in solution and oppositely charged ions on the exchanger matrix. Ion exchange chromatography is commonly performed using column chromatography and takes advantage of the attraction between oppositely charged stationary and mobile phases.
Ion exchange chromatography
Ion exchange chromatography is a technique used to separate charged molecules based on their interaction with oppositely charged groups on a resin. It works by reversible exchange of ions between the ions in a sample and those on an ion exchange resin. There are two types of resins - cation exchange resins which interact with positively charged ions, and anion exchange resins which interact with negatively charged ions. The process involves equilibrating the resin, applying the sample, washing unbound molecules, and then eluting the bound molecules using an increasing salt gradient. Ion exchange chromatography is widely used to purify proteins and analyze ions in applications like biochemistry, water quality testing, and metal purification.
Ion exchange chromatography .ppt
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Synthesis and properties of Polyaniline
This document summarizes the synthesis and properties of polyaniline. Polyaniline was prepared through chemical and electrochemical polymerization in acidic medium. Different solvents, including DMF and m-cresol, were compared for their effect on polyaniline's conductivity. UV-vis spectroscopy and cyclic voltammetry were used to analyze the polymer films. The conductivity of polyaniline was influenced by acidity and the electronic structure of different solvents, which impacts the polymer chain conformation. Polyaniline in m-cresol had higher conductivity than in DMF due to stronger interactions between adjacent polarons.
Ionexchange appln
This document discusses ion exchange, the process by which similarly charged ions can be separated using an ion exchange resin. It describes the principles of cation and anion exchange and how ions are reversibly exchanged between the solution and resin. Different types of ion exchange resins are classified based on their chemical nature and source. The document outlines factors that affect ion exchange separations and provides examples of applications such as water softening and separation of inorganic/organic/biochemical compounds.
Ion Exchange chromatography by Mr. Vinayak
Ion exchange chromatography is a process that separates charged ions using an ion exchange resin. The resin exchanges ions from a sample solution for ions on the resin surface according to their relative affinities. There are two main types of resins - cation exchange resins that attract positively charged ions and anion exchange resins that attract negatively charged ions. The separation occurs through reversible ion exchange between the ions in the sample and resin as the sample passes through a column packed with the resin. Ion exchange chromatography has applications in water softening, enzyme extraction, and purification and separation of ions, sugars, amino acids and proteins.
Lc nmr
HPLC Chromatography and NMR Spectrometry are analytical techniques. HPLC uses different principles like adsorption, ion-exchange, and size-based separation to separate mixtures. It works by pumping a mobile phase through a stationary phase column. NMR spectroscopy uses strong magnetic fields and radio waves to analyze nuclear spin properties to determine molecular structure. Both techniques have various applications in chemistry, forensics, environmental analysis, and quality control. LC-NMR coupling combines the separation power of liquid chromatography with the structural information of nuclear magnetic resonance for analyzing complex mixtures.
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Metal Ion Lab Introduction
- 1. Introduction The objective of this lab experiment is to separate species of Fe(II), Ni(II) and Cu(II) using ion exchange chromatography and to determine the effectiveness of the separation using visible spectroscopy. Ion exchange chromatography relies on charge-charge interactions between the ion(s) in the sample and the charges immobilised on the resin used. In ion exchange chromatography, the stationary phase (resin) consists of a charged polymeric matrix with active or counter-ions that will exchange reversibly with other ions in a surrounding solution. The electric charge carried by the polymer is effectively neutralised by the charges on the counter ions. Ion exchange chromatography can be divided into cation exchange chromatography, in which the ion exchanger or stationary phase (resin) is a polymeric anion with active cations that exchange reversibly with cations in solution; and anion exchange chromatography which uses a polymeric cation with active anions for separation. A strong anion exchange resin is used in this experiment to separate the species. A qualitative determination of concentration of the species and the effectiveness of the separation achieved by the ion exchange chromatography is tested using visible spectroscopy. This procedure and the calculation of various parameters enable the analyst to determine the differences in affinities of the separating species for the resin used and their responses to changes in the composition of the mobile phase. 2