This document is an assignment report on mass spectrometry techniques. It begins with an introduction that defines mass spectrometry as a technique used to quantify known materials, identify unknown compounds, and elucidate molecular structures. The basic principle is described as the study of charged molecules and fragment ions produced when a sample is exposed to ionizing conditions, and the mass-to-charge ratio spectrum that results. The document then covers the advantages and disadvantages of mass spectrometry, describes the typical instrumentation including sample introduction, ionization source, mass analyzer and detector, and discusses applications in various fields like pharmaceutical, clinical, and environmental analysis. It concludes by emphasizing the usefulness of mass spectrometry for studying charged molecules and identifying unknown

1. BIOANALYTICAL TECHNIQUES
ASSIGNMENT
ASSIGNMENT TOWARDS PARTIAL FULFILMENT OF
2nd Internal Examination
ASSIGNMENT REPORT FOR SEMESTER –IV
M.Sc. ZOOLOGY(2ND year)
NAME-AKANKSHA ROUT
REG. NO.-190705180139
SECTION – B
CAMPUS –BBSR
GUIDED BY- Mr. Bibhudutta Mishra

2. MASS SPECTROMETRY
IN BIOANALYSIS
Akanksha Rout
Centurion University Of Technology and Management , BBSR
Core Course-Bioanalytical techniques
M.Sc Zoology (4th semester)
2019-2021
Sec B
Registration no:190705180139

3. CONTENTS
1. • INTRODUCTION
2. • BASIC PRINCIPLE
3. • ADVANTAGES AND DISADVANTAGES
4. • INSTRUMENTATION
5. • APPLICATIONS
6. • CONCLUSION
7. • ACKNOWLEDGEMENT

4. WHAT IS MASS SPECTROSCOPY???
1.INTRODUCTION
• Mass spectrometry is apowerful analytical techniqueused toquantify known materials, to
identifyunknown compoundswithin a sample and toelucidate the structure and chemical propertiesof
different molecules.
• Thus, it is the study of the charged molecules and fragment ions produced from a sample exposed to
ionizing conditions and also of the relative intensity spectrum which results from the correlation of the
ions with theirmass to charge ratio.
• A micro-analytical technique requiring only a fewnano-moles of the sample to obtain characteristic
information pertaining to the structure and molecular weight ofanalyte. This technique basically
studies theeffect of ionizing energy on molecules.
• → Now a day's mass spectrometry isused in manyareasincludingpharmaceutical,
clinical, geological, biotechnology and environmental.
• This unique concept was first introduced by scientists : Sir J. J. Thomson & Wilhelm Wien in
1898.

5. 2. PRINCIPLE

7. ADVANTAGES DISADVANTAGES
• Molecular weight &
formula analysis
• Sample destruction
• Qualitative and
Quantitative analysis
• Sample always desired
to be in the gaseous
form.
• Less amount of sample is
taken.
• Complex and expensive
or high cost not cost
effective
• Less than 1 minute for
analysis.
• Should maintain vacuum
throughout the process.

8. INSTRUMENTATION
WHAT IS MASS SPECTROMETER???
INSTRUMENT

9. INSTRUMENTATION
WHAT ARE ITS COMPONENTS????
INSTRUMENT

10. CONCLUSION
• Mass spectrometry is thus, used for the study of charged
molecules and fragment ions.
• Thus, it is useful for the identification of an unknown compound.

11. APPLICATIONS
Molecular weight , formula and elemental composition
and determination
Identification of an unknown compound
Distinction between cis and trans isomers
Identification of fragmentation patterns
Impurity detection
As LC-MS in analysis of proteins

12. CONCLUSION
• Mass spectrometry is thus, used for the study of charged molecules and
fragment ions.
• Thus, it is useful for the identification of an unknown compound.
• Thus, showing its high applicability and usage in the field of :
 Trace Gas Analysis
 Pharmaceutical Industry
 Space exploration
 Forensic Toxicology
 Archaeological Dating

13. ACKNOWLEDGEMENT
I would like to express my special thanks of gratitude to my Dean Dr.
Yashaswi Nayak as well as the HoD Dr. Sitaram Swain (Head of the
Department) as well as our subject teacher Mr. Bibhudutta Mishra who gave
me the golden opportunity to do this wonderful project on the topic Mass
Spectroscopy, which also helped me in doing a lot of research and it helped to
know about so many new things.
I am really thankful to them.
Secondly I would also like a thank my Department friends who helped me
lot in finishing this project within the limited time and resources.It helped me
increase my knowledge and skills.
*THANKS AGAIN TO ALL WHO SUPPORTED*