This document outlines the methodology for analyzing elements in plant leaves through both dry and wet analysis. Dry analysis uses an elemental analyzer to measure nitrogen, while wet analysis involves ashing leaves in a furnace to convert organic material to inorganic ash that can then be analyzed using an ICP instrument. The wet analysis methodology further describes preparing an ash solution, then a process for analyzing silica levels using a spectrophotometer.

1. MOHAMAD RAAFI B AHMAD
TAIB
152036
(Practical Report Presentation)

3. PRINCIPAL
Dry (Bomb) analysis
 Instrument use Elemental Analyzer (EA)
 For nitrogen
Wet analysis (solubility)
 Organic material need to be converted to
inorganic material
 Ashing (greyish-white)
 Instrument use furnace and ICP
 The elements contain in the leaves can be
diluted using dilute acid.

4. Methodology
(MS 667:Pt. I-VIII:1980)

5. ASHING AND PREPARATION OF
ASH SOLUTION
 Leaf need to be dry and grind to get homogenous.
 Weigh about 1 to 2 g of the sample into a silica
dish.
 Place the dish in the furnace (500⁰C for 5 hours)
until greyish-white

6.  Add 2 ml Hydrochloric acid and evaporate to dryness
on a hot plate.
 Add 10 ml of nitric acid and digest on a water-bath
for 1 hour.

7.  Filter into a 100 ml volumetric flask using filter
paper.
 Make up to volume with water and mix well.

9. PRINCIPAL
Wet analysis (solubility)
 Inorganic material.
 Use spectrometer uv to get the wavelength of
silica (yellow colour).

10.  Weight sample 0.1g ,put in 250 ml
Erlenmeyer flask
 Add 5g Sodium carbonate
 Add 100ml distilled water
 Boil on Hot plate for 15-30 min
ANALYSIS OF SILICA

11.  Pour 5 ml sulfuric acid
 Boil in water bath (6 hours), leave 2 days
 Filter samples & dilute 10 times
 Pipette 10 ml sample & add molybdate, acid
reagent and citric acid (shake)
 Obtain result from spectrophotometer
(410 nm yellow wavelength)