The document outlines various lab skills developed across several chemistry, biochemistry, microbiology, and senior research courses, including techniques such as titrations, spectroscopy, chromatography, organic synthesis, microbiological assays, protein purification, cell culture, and mass spectrometry analysis. Key skills involve the safe use of lab equipment and glassware, preparation of samples and media, separation and analytical methods like titration, spectroscopy, and chromatography, and techniques for protein analysis, DNA work, and organic synthesis.

1. Lab Skills
 Gen ChemI& II (102, 104) withlab
o Lab Safety
o Glassware andinstrumentationuse
o Titrations
o Potentiometry
o Fluorometry
o pH meter
o Scale
 OrganicChemI & II and lab(343, 344, 345)
o OrganicSynthesis
o Volumetrictechniques
o Solutionprep
o Extraction
o Filtration(vacuum)
o Separation
o Sublimation
o Distillation
o Chromatography(column,GC,TLC)
o Spectroscopyanalysis:x-ray,IR,UV,visible,NMR,massspectroscopy,Raman
o Columnpacking
o Centrifuge
o Reflux
o Recrystallization
o Rotary evaporator
o Meltingpoint/boilingpointdetermination
 Quantitative Analysis(221) withlab
o Gravimetricanalysis
o Titrimetricprecipitation
o Titrimetry:Monoproticandpolyproticacidsandbases,metal-ioncomplexation,
oxidation-reductionreactions
o Coulometricmethods
o Potentiometry
o Molecularspectroscopy
o Gas and liquidchromatography
o Fluorimetric/colorimetricdetermination
 Microbiology(383) withlab
o Bacterial Staining
o Platingmethods
o Graphinggrowthcurves
o Bacterial identification
o Microscope application(oil immersion)
o Mediaand bufferprep
o Selectivemedia

2. o Conjugation
o Antibioticassay,plaqueassay,Amestest
 BiochemistryLab(603)
o Batch cultures
o DNA extraction
o PlasmidDNA prep
o Restrictionenzymedigests
o PCR (qPCR,RT-PCR)
o Gel electrophoresis
o SDS-PAGE
o Differential Centrifugation
o Microarray Interpretation
o ProteinAssay(Lowry,Bradford,ligandbinding)
o Western/Northern/Southernblotting
o ProteinPurification (chromatography,ion-exchange)
 BiochemistryProteinStructures(601)
o Proteinmodelingsoftware(pymol)
o BLAST
o FRET interpretation
 SeniorResearch(691)
o Organicsynthesisof drugsfollowinglab notes
o Mice injections
o Rotary evaporator
o Reflux
o TLC plate for productyielddetermination
o Columnpreparationforsample separation
o Centrifugation
o Human and mice cell culturesfordrug interactions
o Drug metabolism(DMPK) assayprep
o Scanningmetaboliteprofilesusingmassspectrometry