The document describes various organelles and structures observed in electron microscopy images of cells and tissues, including mitochondria, the endoplasmic reticulum, lysosomes, centrioles, the Golgi complex, peroxisomes, lipid droplets, and glycogen granules. Key details are provided about the appearance, location, and functions of these organelles and inclusions.

1. CYTOLOGY Lab.

2. 1. Single mitochondria, RER & Lipid droplet.
2. Mitochondria ,RER & Secondery lysosomes.
3. Mitochondria, SER & glycogen granules.
4. T.S of centriole , Golgi complex &coated vesicle.
5. Golgi complex.
6. Peroxisome & Golgi complex.
7. Golgi apparatus in epididymis

3. 8. Nucleus & RER (EM).
9. Nuclear pore & rER continuous with the outer
nuclear membrane(EM).
10. Melanin pigment in negroid skin
11. Glycogen granules in liver. Slide
12. lipid droplet.(EM)

4. Single mitochondria, rER
& lipid droplet
Mitochondria.
Outer membrane and inner folded
membrane.
Cristae
Rough endoplasmic reticulum :
Consists of stacks of flattened
cisternae.
Membranous organells.
Polyribosomes on cytosolic surface of
the membrane

5. Mitochondria, rER & secondary lysosomes
Secondary
lysosomes.
Membranous
cell organelles..
Appears
heterogenous
due to presence
of digested
materials

6. sER , Mitochondria &
glycogen granules
Smooth endoplasmic reticulum.
Absence of ribosomes (smooth)
Cisternae is tubular in shape (not
flattened)
Mostly found in cells synthesizing steroid
hormones, cholesterol and triglycerides
like liver and endocrine cells.
Glycogen granules: appears
as collections
of electron-dense
particles (rosett appearance)

7. T.S. of centriole, Golgi
complex & coated vesicle
Centrioles are non-membranous
organelles.
centriole is a hollow cylinder with a dense
wall around an electron lucent central core.
Its wall is made up of 9 bundles of
microtubules, each bundle consists of 3
microtubules (triplets).

8. Golgi complex
Cis face and trans face, transfer
vesicles secretory vesicles.
Cis-face (immature or forming face)
which is usually convex. It is directed
towards rough endoplasmic reticulum
and its cisternae are highly
fenestrated.
Trans-face (mature or secretory face)
is usually concave and is directed
towards the plasmalemma. The lumen
of its cisternae tends to be wider.

9. 6- Nucleus & rER NUCLEUS: The nuclear
envelope, chromatin, nucleolus, and
nuclear matrix.

10. Peroxisome & Golgi complex
Spherical membranous organelles.
Have a dense central region called crystaline core.
Fine granular homogenous content.

11. Lipid droplets & cell membrane
A cell inclusion, Electron dense and homogenous, Variable in size.

12. Nuclear pores & rER continous with the outer
nuclear membrane

13. Golgi apparatus in epididymis
Dark stained area in cells of epididymis --- Supranuclear in position

14. Glycogen granules in liver
A cytoplasmic inclusion, present in large amounts in liver cells
(hepatocytes). Glycogen appears as dark acidophilic granules

15. Melanin pigments in negroid
skin
A cytoplasmic deposit - Dark brown pigment. - In the
epidermis of skin.