This document discusses validating and enhancing reference materials from the Genome in a Bottle (GiaB) Consortium. 1) MetaSV trio analysis validated over 98% of GiaB deletion calls for sample NA12878, and identified additional high-quality calls not currently in the GiaB gold set. 2) MetaSV ensemble approach and optimized assembly helps refine structural variant breakpoints. Applying this to a Jewish trio can help enhance the GiaB gold set. 3) Over 75% of high-confidence MetaSV calls overlap with calls from the Parliament SV caller, demonstrating consistency between methods.