This document describes the development of an instrument to measure 21st century skills among Malaysian secondary school students in biology. The researchers identified five key components of 21st century skills - digital age literacy, inventive thinking, effective communication, high productivity, and spiritual values. They developed 82 questionnaire items measuring these components and field tested them with 433 students. Analysis using the Rasch model showed the instrument had very good reliability. However, 11 items were identified as misfit and need revision. The developed instrument will allow assessment of students' existing 21st century skills and help teachers improve instruction to better develop these important skills.