Calibration of infrared (IR) spectroscopy ensures the instrument provides accurate measurements. The calibration process involves using a polystyrene film standard to produce an IR spectrum. The spectrum is compared to a reference standard to check if the strongest peak is within 95% of the maximum. If not, adjustments are made to correct the signal. Periodic calibration is needed to account for instrument drift over time and ensure precise results.

1. Submitted by:-
Vishnu
1704120033
B.Pharma 6th sem.

2.  CALIBRATION
 INFRARED SPECTROSCOPY
 CALIBRATION OF IR SPECTROSCOPY
 IR SPECTROSCOPY CALIBRATION PROCEDURE

3. CALIBRATION
Calibration is a process by which ensure that an instrument readings are accurate
with reference to established Standards. Calibration is a comparison between a
known measurement and measurement using your instrument.
The most precise measurement instrument is of no use if you cannot be sure that
it is reading accurately. There are some definitions on the error of measurement
is:
• Calibration range:- the region between the within which a quantity is measured,
received or transmitted which is expressed by stating the lower and upper range
values
• Instrument range:- the capability of the instrument may be different than the
calibration range

4. Calibration required because of:
Many instruments or components having changes due to some mechanical
stress or another physical parameters and perform degradation in the
instruments that degradation is known as “Drift” at that time an instrument
looses its ability. So there is calibration needed.

5. INFRARED SPECTROSCOPY
It also known as IR spectroscopy and vibrational spectroscopy.
IR spectroscopy generally refers to the analysis of interaction of molecule with
infrared light. IR spectroscopy is mainly based on absorption spectroscopy It
can be used to identify and study chemical substances.
The technique or method of IR spectroscopy is conducted with an instrument
called IR spectrophotometer to produce an IR spectrum.
Some common laboratory instrument that used by this technique i.e.
FTIR (Fourier transform infrared spectrophotometer) which can accurately
identify compounds such as compounded plastics, fillers, paints, rubber,
coating, resins and adhesives.

6. Calibration of IR spectroscopy
Whenever use any technical instrument, make sure that the
instrument is in proper working or not. To ensure proper working of
instrument should use known sample and calibrate the instrument.

7. IR Spectroscopy calibration procedure:
• A instrument spectrophotometer requires calibration periodically,
which is the first step to using spectrophotometer to warm up
correctly. The wavelength of IR
spectrophotometer is usually carried out with the strip of
polystyrene film as a standard.
• take a piece of polystyrene in sample holder scan the instrument
and verify the peak in IR spectra.
• Allow the spectrophotometer warm up for 10 min. so that
spectrophotometer Stabilizes to produces accurate result and
results depends on source radiation of sample.

8. Continue…..
• The sample can also be examined as solution in solvent such as
Ccl4 or chloroform solution and the 2nd cell contain pure solvent.
• Place the polystyrene film on the sample holder and without test
use of sample of known spectra called a standard.
• Retrieve the spectra for polystyrene sample than compare the
spectra with standard reference and also ensure the peak of
spectra during test.
• than the peak are compare with absorptive wavelength and check
the spectra to ensure that the signal or wave is within 95% of
maximum of strongest peak.

9. • If the strongest peak in the spectra is more or less than scale than
adjust the signal as to correct the reduction of adjustment.
• many times spectrophotometer should have calibration. Just before
and after your work a minimum frequency of calibration is needed.