1) Antibodies are antigen-binding proteins produced by B cells and plasma cells in response to antigens. The immune system responds to antigens by producing antibodies that target multiple epitopes on the antigen from several recruited B cell clones.
2) There are five major classes of antibodies (immunoglobulins): IgG, IgM, IgA, IgE, and IgD. Each class has unique structural and functional properties conferred by their constant region amino acid sequences.
3) IgG is the most abundant immunoglobulin and exists as four subclasses that differ in chain sequences and effector functions like complement activation and binding to Fc receptors. IgM is the first antibody produced and most efficient at activating complement.
immunoglobulin are produce by the body against specific stimuli i.e antigen. antibody are of different types and these are classify according to there nature, temperature of reaction, structure and crossing of placenta. antibody are of Y shaped structure which is composed of different region. classification is based on the type of heavy chain, there are 5 major classes of antibodies, IgA, Igd, Igm, ige, most important of these are igm and igg, although each and every have there own significance.
OUTCOMES
By the end of this session student should be able to know
The structure of antibody
Immunoglobulin classes
Monoclonal antibodies VS polyclonal
INTRODUCTION
Antibodies are globulin proteins (immunoglobulins [Ig]) that react specifically with the antigen that stimulated their production.
They make up about 20% of the protein in blood plasma. Blood contains three types of globulins,
alpha,
beta,
gamma,
Antibodies are gamma globulins.
INTRODUCTION
There are five classes of antibodies:
1. IgG,
2. IgM,
3. IgA,
4. IgD,
5. IgE
Antibodies are subdivided into these five classes based on differences in their heavy chains.
ROLE OF ANTIBODIES
The most important functions of antibodies are to
neutralize toxins and viruses,
to opsonize microbes
so they are more easily phagocytosed, to activate complement, and to prevent the attachment of microbes to mucosal surfaces.
In addition to these functions, antibodies have a catalytic (enzymatic) capability
Antibody Type
IgA
IgD
IgE
IgG
IgM
Function
Found in saliva, tears, mucus, breast milk and intestinal fluid, IgA protects against ingested and inhaled pathogens.
This antibody is found on the surface of your B cells. Though its exact function is unclear, experts think that IgD supports B cell maturation and activation.
Found mainly in the skin, lungs and mucus membranes, IgE antibodies cause your mast cells (a type of white blood cell) to release histamine and other chemicals into your bloodstream. IgE antibodies are helpful for fighting off allergic reactions.
This is the most common antibody, making up approximately 70% to 75% of all immunoglobulins in your body. It’s found mainly in blood and tissue fluids. IgG antibodies help protect your body from viral and bacterial infections.
Found in your blood and lymph system, IgM antibodies act as the first line of defense against infections. They also play a large role in immune regulation.
MONOCLONAL VS POLYCLONAL
A. Polyclonal antibodies contain a heterologous mixture of IgGs against the whole antigen
B. monoclonal antibodies are composed of a single IgG against one epitope.
Polyclonal antibodies
Monoclonal antibodies
Refer to a mixture of immunoglobulin molecules that are secreted against a particular antigen.
Refer to a homogenous population of antibodies that are produced by a single clone of plasma B cells.
Produced by different clones of plasma B cells.
Produced by the same clone of plasma B cells.
A heterogeneous antibody population.
A homogenous antibody population.
Interact with different epitopes on the same antigen.
Interact with a particular epitope on the antigen.
STRUCTURE OF ANTIBODY
Immunoglobulins are glycoproteins made up of
1. light (L)
2. heavy (H) polypeptide chains.
The terms light and heavy refer to molecular weight
STRUCTURE OF ANTIBODY
The simplest antibody molecule has a Y shape consist of
immunoglobulin are produce by the body against specific stimuli i.e antigen. antibody are of different types and these are classify according to there nature, temperature of reaction, structure and crossing of placenta. antibody are of Y shaped structure which is composed of different region. classification is based on the type of heavy chain, there are 5 major classes of antibodies, IgA, Igd, Igm, ige, most important of these are igm and igg, although each and every have there own significance.
OUTCOMES
By the end of this session student should be able to know
The structure of antibody
Immunoglobulin classes
Monoclonal antibodies VS polyclonal
INTRODUCTION
Antibodies are globulin proteins (immunoglobulins [Ig]) that react specifically with the antigen that stimulated their production.
They make up about 20% of the protein in blood plasma. Blood contains three types of globulins,
alpha,
beta,
gamma,
Antibodies are gamma globulins.
INTRODUCTION
There are five classes of antibodies:
1. IgG,
2. IgM,
3. IgA,
4. IgD,
5. IgE
Antibodies are subdivided into these five classes based on differences in their heavy chains.
ROLE OF ANTIBODIES
The most important functions of antibodies are to
neutralize toxins and viruses,
to opsonize microbes
so they are more easily phagocytosed, to activate complement, and to prevent the attachment of microbes to mucosal surfaces.
In addition to these functions, antibodies have a catalytic (enzymatic) capability
Antibody Type
IgA
IgD
IgE
IgG
IgM
Function
Found in saliva, tears, mucus, breast milk and intestinal fluid, IgA protects against ingested and inhaled pathogens.
This antibody is found on the surface of your B cells. Though its exact function is unclear, experts think that IgD supports B cell maturation and activation.
Found mainly in the skin, lungs and mucus membranes, IgE antibodies cause your mast cells (a type of white blood cell) to release histamine and other chemicals into your bloodstream. IgE antibodies are helpful for fighting off allergic reactions.
This is the most common antibody, making up approximately 70% to 75% of all immunoglobulins in your body. It’s found mainly in blood and tissue fluids. IgG antibodies help protect your body from viral and bacterial infections.
Found in your blood and lymph system, IgM antibodies act as the first line of defense against infections. They also play a large role in immune regulation.
MONOCLONAL VS POLYCLONAL
A. Polyclonal antibodies contain a heterologous mixture of IgGs against the whole antigen
B. monoclonal antibodies are composed of a single IgG against one epitope.
Polyclonal antibodies
Monoclonal antibodies
Refer to a mixture of immunoglobulin molecules that are secreted against a particular antigen.
Refer to a homogenous population of antibodies that are produced by a single clone of plasma B cells.
Produced by different clones of plasma B cells.
Produced by the same clone of plasma B cells.
A heterogeneous antibody population.
A homogenous antibody population.
Interact with different epitopes on the same antigen.
Interact with a particular epitope on the antigen.
STRUCTURE OF ANTIBODY
Immunoglobulins are glycoproteins made up of
1. light (L)
2. heavy (H) polypeptide chains.
The terms light and heavy refer to molecular weight
STRUCTURE OF ANTIBODY
The simplest antibody molecule has a Y shape consist of
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Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
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The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
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Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
2. Most antigensare complex andcontainmanydifferentantigenicdeterminants andthe immune system
usuallyrespondsbyproducingantibodiestoseveral epitopesonthe antigen.Thisresponse requiresthe
recruitmentof several clonesof Bcells.Theiroutputsare monoclonal antibodies,eachof which
specificallybindsasingle antigenicdeterminant .Together these monoclonal antibodiesmake upthe
polyclonal andheterogeneousserumantibodyresponsetoanimmunizingantigen.
Antibody Classes and Biological Activities
Each class isdistinguishedbyuniqueaminoacidsequencesinthe heavy-chainconstantregionthat
conferclass-specificstructural andfunctional properties.
3.
4. ImmunoglobulinG(IgG)
IgG, the most abundantclassinserum,constitutesabout 80% of the total serumimmunoglobulin.The
IgG molecule consistsoftwoheavychainsandtwolightchain.
lightchains.There are fourhuman IgG subclassesdistinguishedbydifferencesin -chainsequence and
numberedaccordingtotheirdecreasingaverage serumconcentrations:IgG1,IgG2,IgG3,andIgG4 . The
aminoacid sequencesthatdistinguishthe fourIgGsubclassesare encodedbydifferentgerm-line CH
genes,whose DNA sequencesare 90%–95% homologous.The structural characteristicsthatdistinguish
these subclassesfromone anotherare the size of the hinge regionandthe numberandpositionof the
interchaindisulfide bondsbetweenthe heavychains.
IgG1 IgG3 andIgG4 readilycrossthe placentaandplayan importantrole inprotectingthe developing
fetus.
IgG3 isthe most effective complementactivator,followed byIgG1; IgG2 is lessefficient andIgG4 isnot
able to activate complementatall
IgG1 and IgG3 bindwithhighaffinitytoFcreceptorson phagocyticcellsandthusmediate
opsonization.IgG4has an intermediate affinityforFcreceptors andIgG2 has an extremelylow affinity.
ImmunoglobulinM (IgM)
IgMaccounts for 5%–10% of the total serumimmunoglobulin,withanaverage serumconcentrationof
1.5 mg/ml.MonomericIgM,witha molecularweightof 180,000, isexpressedasmembrane-bound
5. antibodyonB cells.IgMissecretedbyplasmacellsasa pentamer inwhichfive monomerunitsare held
togetherbydisulfide bondsthatlinktheircarboxyl-terminalheavychaindomains.
IgMis the firstimmunoglobulinclassproducedinaprimaryresponse toan antigen.
IgMis alsomore efficientthanIgG at activatingcomplement.ComplementactivationrequirestwoFc
regionsinclose proximity andthe pentamericstructure ofasingle molecule ofIgMfulfillsthis
requirement.Because of itslarge size,IgMdoesnotdiffuse well andtherefore isfoundinverylow
concentrationsinthe intercellulartissue fluids.The presence of the JchainallowsIgMto bind to
receptorsonsecretorycells whichtransportitacrossepithelialliningstoenterthe external secretions
that bathe mucosal surfaces.AlthoughIgA isthe majorisotype foundinthesesecretions IgMplaysan
importantaccessoryrole asa secretoryimmunoglobulin.
ImmunoglobulinA(IgA)
AlthoughIgA constitutesonly10%–15%of the total immunoglobulininserum, itisthe predominant
immunoglobulinclassinexternalsecretionssuchasbreastmilk,saliva,tears,andmucus of the
bronchial,genitourinary anddigestivetracts.
6. The dailyproductionof secretoryIgA isgreaterthan thatof any otherimmunoglobulinclass.IgA-
secretingplasmacellsare concentratedalongmucousmembranesurfaces.Alongthe jejunumof the
small intestine,forexample,there are more than2.5 1010 IgA-secretingplasmacells—anumberthat
surpassesthe total plasmacell populationof the bone marrow lymph andspleencombined!Everyday,a
humansecretesfrom5 g to 15 g of secretoryIgA intomucoussecretion.
ImmunoglobulinE (IgE)
The potentbiological activityof IgEallowedittobe identifiedinserumdespite itsextremelylow
average serumconcentration(0.3g/ml).IgEantibodiesmediatethe immediatehypersensitivityreactions
that are responsible forthe symptomsof hayfever,asthma,hives,andanaphylacticshock.The presence
of a serumcomponentresponsible forallergicreactions.
IgE bindsto Fc receptorsonthe membranesof bloodbasophilsandtissue mastcells
7. ImmunoglobulinD(IgD)
IgD has a serum concentration of 30 g/ml and constitutes about 0.2% of the total immunoglobulin in
serum. IgD together with IgM is the major membranebound immunoglobulin expressed by mature B
cells and its role in the physiology of B cells is under investigation.No biological effector function has
been identified for IgD