Autophagy is a normal cellular process that maintains homeostasis through degradation and recycling of cellular components. It plays a dual role in cancer and other diseases. The document discusses three main types of autophagy and the steps of autophagosome formation. It explores the link between autophagy and diseases like cancer, diabetes, and neurodegenerative disorders. Specifically, it examines the role of autophagy in diabetic neuropathy and potential interventions that target the autophagy process.
here is some information about autophagy, how it happend, when it happend and it's mechanism.
and some information about it's effect on cancer and some disorders.
Autophagy the housekeeper in every cellfathi neana
Autophagy is a catabolic process involving the degradation of a cell’s own components through the lysosomal machinery. It is a tightly regulated process that plays a normal part in cell growth, development, and homeostasis, helping to maintain a balance between the synthesis, degradation, and subsequent recycling of cellular products.
It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Autophagy is an evolutionarily conserved mechanism of cellular self-digestion in which proteins and organelles are degraded through delivery to lysosomes. Defects in this process are implicated in numerous human diseases including cancer.
here is some information about autophagy, how it happend, when it happend and it's mechanism.
and some information about it's effect on cancer and some disorders.
Autophagy the housekeeper in every cellfathi neana
Autophagy is a catabolic process involving the degradation of a cell’s own components through the lysosomal machinery. It is a tightly regulated process that plays a normal part in cell growth, development, and homeostasis, helping to maintain a balance between the synthesis, degradation, and subsequent recycling of cellular products.
It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Autophagy is an evolutionarily conserved mechanism of cellular self-digestion in which proteins and organelles are degraded through delivery to lysosomes. Defects in this process are implicated in numerous human diseases including cancer.
A detailed description of programmed cell death mechanism also called Apoptosis.
It explains about the factors, mechanism and pathways involved in the apoptosis.
MULTIFACETED ROLES OF AUTOPHAGY IN CANCER & NEURODEGENERATIVE DISORDERSPHARMA IQ EDUCATION
1. What is Autophagy
2. Overview of the mechanisms lying behind autophagy
3. Mechanism of autophagy
4. Physiological role of autophagy
5. Role of Autophagy in cancer
6. Autophagy in tumor suppression
7. Autophagy in tumor promotion
8. Role of Autophagy in neurodegenerative diseases
9. Mechanism of autophagy in neurodegenerative disorders
10. Conclusion
11. References
12. Thank You
Introduction
Definition
History
Evolution and origin of apoptosis
Significance
Purpose of apoptosis
Steps /process
Morphological and biochemical changes
Mechanism of apoptosis
Caspases
Regulation of apoptosis
Disorders of apoptosis
Application
Conclusion
Referances
Membrane proteins are proteins that interact with, or are part of, biological membranes. They include integral membrane proteins that are permanently anchored to the membrane and peripheral membrane proteins which are only temporarily attached to the lipid bilayer or to integral proteins.
This presentation illustrates the various pathways of development of AD ,including the recent molecular pathways , and their implication in early diagnosis and therapy .
A detailed description of programmed cell death mechanism also called Apoptosis.
It explains about the factors, mechanism and pathways involved in the apoptosis.
MULTIFACETED ROLES OF AUTOPHAGY IN CANCER & NEURODEGENERATIVE DISORDERSPHARMA IQ EDUCATION
1. What is Autophagy
2. Overview of the mechanisms lying behind autophagy
3. Mechanism of autophagy
4. Physiological role of autophagy
5. Role of Autophagy in cancer
6. Autophagy in tumor suppression
7. Autophagy in tumor promotion
8. Role of Autophagy in neurodegenerative diseases
9. Mechanism of autophagy in neurodegenerative disorders
10. Conclusion
11. References
12. Thank You
Introduction
Definition
History
Evolution and origin of apoptosis
Significance
Purpose of apoptosis
Steps /process
Morphological and biochemical changes
Mechanism of apoptosis
Caspases
Regulation of apoptosis
Disorders of apoptosis
Application
Conclusion
Referances
Membrane proteins are proteins that interact with, or are part of, biological membranes. They include integral membrane proteins that are permanently anchored to the membrane and peripheral membrane proteins which are only temporarily attached to the lipid bilayer or to integral proteins.
This presentation illustrates the various pathways of development of AD ,including the recent molecular pathways , and their implication in early diagnosis and therapy .
This presentation introduces a brief and rapid review for an important research area (oxidative stress) and its relation to liver fibrosis.
Liver fibrosis is very important for us as we are facing a very dangerous and continuously growing problem in Egypt, HEPATIC PATIENTS COMPLICATIONS.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
Nucleophilic Addition of carbonyl compounds.pptxSSR02
Nucleophilic addition is the most important reaction of carbonyls. Not just aldehydes and ketones, but also carboxylic acid derivatives in general.
Carbonyls undergo addition reactions with a large range of nucleophiles.
Comparing the relative basicity of the nucleophile and the product is extremely helpful in determining how reversible the addition reaction is. Reactions with Grignards and hydrides are irreversible. Reactions with weak bases like halides and carboxylates generally don’t happen.
Electronic effects (inductive effects, electron donation) have a large impact on reactivity.
Large groups adjacent to the carbonyl will slow the rate of reaction.
Neutral nucleophiles can also add to carbonyls, although their additions are generally slower and more reversible. Acid catalysis is sometimes employed to increase the rate of addition.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptxMAGOTI ERNEST
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
2. CONTENTS
12/9/2017AUTOPHAGY LINKS TO VARIOUS DISEASES2
Introduction
Types of autophagy
Dual role of autophagy
Autophagy in cancer cells
Autophagosome formation
Diseases and autophagy role
Role of autophagy in diabetes and diabetic complications
Role of autophagy in diabetic neuropathy
3. Introduction
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Definition:
• Autophagy is a normal physiological process in the
body that deals with destruction of cells in the body.
• It maintains homeostasis or normal functioning by protein
degradation and turnover of the destroyed cell organelles
for new cell formation.
4. Types of Autophagy
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There are three different forms of autophagy that are
commonly described
1. Macroautophagy
2. Microautophagy
3. Chaperone – mediated autophagy
Micro Autophagy
Cytosolic components are directly taken up by the
lysosome itself through invagination of the lysosomal
membrane.
Macro Autophagy
Delivery of cytoplamic cargo to the
intermediatery of a double membrane bound vesicle ,
referred as an autophagosome, that fuses with the
5. Conti…
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5
Chaperone – Mediated Autophagy
Targeted proteins are traslocated across the
lysosomal membrane in a complex with chaperone
proteins (such as Hsc-70) that are recognised by the
lysosomal membrane receptor LAMP-2A, resulting in
their unfolding and degradation
6. Autophagy Role In Cancer
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Autophagy is a survival-promoting pathway that captures, degrades,
and recycles intracellular proteins and organelles in lysosomes.
Although in some contexts autophagy suppresses tumorigenesis ,
and most contexts autophagy facilitates tumorigenesis.
Cancers can up regulate autophagy to survive micro environmental
stress and to increase growth and aggressiveness.
Mechanisms by which autophagy promotes cancer include
suppressing induction of the p53 tumour suppressor protein and
maintaining metabolic function of mitochondria.
7. ssssssssssssssss0
z
Conti....
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AUTOPHAGY LINKS TO VARIOUS
DISEASES
7
Tumour suppression by Autophagy in mouse liver
Autophagy deficiency
( Atg7or Becn1 Atg5)
P62accumulation
Mitochondrial defects
Oxidative stress
DNA damage
Cell death
Chronic tissue damage
Inflammation
Oncogenic signaling
Genome instability
Tumour initiation
Tumour promotion by Autophagy
Deregulated prolifiration
(KRASG12D or BRAFV600E)
Autophagy
Oxidative stress
ER stress
Mitochondrial function
Metabolism
Stress tolerance
Tumour promotion
zz
8. AUTOPHAGOSOME FOMATION
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DISEASES
8
Five steps are involved in the autophagic pathways have been
identified:
1. Nucleation
2. Elongation
3. Closure
4. Fusion
5. Degradation
Nucleation
Isolated membrane appears in a cytosol.
Elongation
It is characterized by membrane bending and increase in the
size of the phagophore.
Closure
The autophagosome membrane wraps around the cytosolic
components.
Fusion
The fusion of autophagosome with a lysosome to form an
autolysosome.
10. Diseases and Autophagy
role
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AUTOPHAGY
CANCER
CELL DEATH
INNATE IMMUNE
SYSTEM
CARDIOMYOPATHY
TYPE II DIABETES
AGING
INFECTIOUS DISEASES
NEURODEGENERATIVE
DISEASES
11. ROLE OF AUTOPHAGY IN
DIABETES AND DIABETIC
COMPLICATIONS
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13. Role of Autophagy in Diabetic
Neuropathy
12/9/2017AUTOPHAGY LINKS TO VARIOUS DISEASES13
Role of autophagy in diabetes can by done by two
ways
1. Protein aggregation and Endoplasmic
Reticulum(ER) stress in Diabetic
Neuropathy(DN).
2. Oxidative stress and mitotoxicity in Diabetic
Neuropathy(DN).
14. Protein aggregation and ER stress in
DN
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• As the path physiology of DN revolves around one of the unifying
mechanism i.e., reactive oxygen species (ROS) resulting in oxidative
stress, there exists a propensity of protein misfolding and aggregation.
• Proteins are highly susceptible to the modifications by ROS ROS
induced protein modifications might be reversible such as S-
glutathionylation and S-nitrosation.
• But irreversible protein modifications such as carbonylation results in
protein misfolding and aggregation depending on the severity of
• carbonylation.
• Both ER and Ubiquitin proteasome system (UPS) acts as quality control
systems for proteins deregulated under hyperglycemic conditions .
• ER requires reduced environment for oxidative folding of proteins.
• The reduced environment is maintained by antioxidants such as reduced
glutathione.
15. Oxidative stress and Mitotoxicity in
Diabetic Neuropathy
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• Mitochondrial dysfunction mainly occur either due to
synthesis of damaged electron transport chain(ETC)
components or due to enhanced ROS generation from
ETC.
• Later one occurs due to enhanced supply of NADH to ETC
which causes partial reduction of molecular oxygen to
superoxide in hyperglycemic state.
• It was well proved from the studies on diabetic rats that
hyperglycemia caused impairment in the function of ETC
enzyme complexes and some enzymes of tricarboxylic
acid(TCA) cycle.
As a result of failure of mitochondrial function and
metabolism reduced ATP generation occurs and thus leads
to bioenergetic crisis followed by necrosis .
16. Conti....
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Mitochondrial dysfunction in hyperglycemia is also
associated with Ca2+ dyshomeostasis.
Elevated levels of intramitochondrial Ca2+ levels make
mitochondria to work faster by stimulating TCA enzymes and
thus enhances the generation of ROS further .
19. Repurposed Drug Targets in Autophagy
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Rapamycin or chloroquine are studied as autophagy modulators in
clinical trials for lung diseases.
For example : chloroquine, which inhibits autophagy-mediated cell
survival in tumour cells, is used as an intervention for patients with
small cell lung cancer in a clinical trial.
Interventions using hydrochloroquine and other anticancer drugs such
as erlotinib are used for non-small cell lung cancer in two clinical trials.
paradox may used due to the differential roles of autophagy in
different stages of tumorigenesis .
20. Conti….
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Lymphangioleiomyomatosis (LAM), a progressive lung
disease caused by mutation in the tuberous sclerosis
genes (tsc), is associated with inappropriate activation of
mTOR signaling, which regulates cellular growth and
lymphangiogenesis
For example : Chloroquine is also used with rapamycin for
patients with LAM.