The document summarizes resistance and immune responses to infectious diseases. It discusses the four main types of pathogens (viruses, bacteria, protozoa, helminths) and provides details on immune responses to specific pathogens like influenza virus, diphtheria bacteria, malaria protozoa (Plasmodium), and parasitic worms. It also notes that microbes have evolved ways to evade the immune system, such as antigenic variation, hiding in protected niches, and suppressing immune responses.
CLONAL SELECTION THEORY IS AN SCIENTIFIC THEORY IN IMMUNOLOGY THAT EXPALINS THE FUNCTION OF CELLS OF THE IMMUNE SYSTEM IN RESPONSE TO SPECIFIC ANTIGEN INVADING THE BODY.
Antibodies are immune system-related proteins called immunoglobulins. Each antibody consists of four polypeptides– two heavy chains and two light chains joined to form a "Y" shaped molecule. ... This variable region, composed of 110-130 amino acids, give the antibody its specificity for binding antigen.
CLONAL SELECTION THEORY IS AN SCIENTIFIC THEORY IN IMMUNOLOGY THAT EXPALINS THE FUNCTION OF CELLS OF THE IMMUNE SYSTEM IN RESPONSE TO SPECIFIC ANTIGEN INVADING THE BODY.
Antibodies are immune system-related proteins called immunoglobulins. Each antibody consists of four polypeptides– two heavy chains and two light chains joined to form a "Y" shaped molecule. ... This variable region, composed of 110-130 amino acids, give the antibody its specificity for binding antigen.
Parasitic infection and immunomodulation: A possible explanation for the hygi...Apollo Hospitals
Helminthic parasites have a long history of co-evolution with human beings. The incidence of helminthic infection has significantly decreased in developed countries due to better sanitary measures. However, epidemiological data suggest a corresponding increase in the incidence of autoimmune and allergic diseases in association with a reduction in helminthic infections in these societies. The immune response to helminthic infection involves both innate and adaptive processes, with a strongly polarised Th2 response being the most characteristic feature. However, there is a concomitant increase in the functional regulatory T cell responses. This might explain the paradoxical decrease in both Th2-and Th1-mediated diseases such as allergy and immune-mediated inflammatory disorders in populations with increased incidence of helminthic infection. Parasitic infection therefore appears to confer a degree of immunomodulation, and for this reason, utilising helminthic infection as a therapeutic modality for the treatment of allergic and autoimmune disease has been proposed. Improved understanding of the immunologic responses to helminth infection allows these mechanisms to be exploited, enabling manipulation of the immune response in Th1-dominant conditions such as inflammatory bowel disease and multiple sclerosis, and providing a new approach to treatment of these and other inflammatory and allergic conditions.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
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A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
4. INTRODUCTION
Despite innate and adaptive immune responses to
pathogens, infectious diseases which have plagued
human populations throughout history still cause
millions of deaths per year.
There are 4 main types of pathogens that cause
infectious diseases:
Viruses
Bacteria
Protozoa
Helminths
5. VIRAL INFECTION
The immune response to viral infections involve both:
Humoral immunity
Cell-mediated components
Antibody to a viral receptor can block viral infections of host cells. However, a number of viruses,
including influenza, are able to mutate their receptor molecules and thus evade the humoral
antibody response.
Once a viral infection has been established, cell-mediated immumity appears to be more important
than humoral.CD8 Tc and CD4 TH cells are the main components of cell-mediated antiviral defense.
6. INDUCTION OF ANTIVIRAL ACTIVITY
Induction of antiviral activity by IFN-α and IFN-β.
These interferons bind to the IFN receptor, which in turn induces the synthesis of both 2-
5(A) synthetase and protein kinase (PKR).
The action of 2-5(A) synthetase result in the activation of RNAse L, which can degrade
messanger RNA.
PKR inactivates the translation inititation factor elF-2 by phosphorylating it.
Both pathways thus result in the inhibition of protein synthesis and thereby effectively
block viral replication.
8. INFLUENZA VIRUS
The influenza virus infects the upper respiratory tract and major
central airways in humans, horses, birds etc.
The envelope is covered with neuraminidase and hemagglutinin
spikes, which are responsible for the attachment of the virus to the
host cells.
Inside is an inner layer of matrix proteins surrounding the
nucleocapsid, which consist of eight ssRNA molecules associated
with nucleoprotein.
The eight RNA strands encode ten proteins: PB1, PB2, PA, HA
(hemagglutinin), NP (nucleoprotein), NA (neuraminidase), M1,
M2, NS1 and NS2.
9. HOST RESPONSE TO INFLUENZA
ACTION
Two mechanism generate variations in influenza
surface antigens.
In antigenic drift, the accumulation of point
mutations eventually yield a variant protein that is
no longer recognized by antibody to the original
antigen.
Antigenic shift may occur by reassortment of an
entire ssRNA between human and animal virions
infecting the same cell. Only four of the eight RNA
strand are depicted.
10. BACTERIAL INFECTIONS
Bacteria enter the body either through a number of natural routes (e.g., the respiratory tract, the
gastrointestinal tract etc.) or through normally inaccessible routes opened up by breaks in mucous
membranes or skin.
There are four primarily steps in bacterial infection:
Attachment to host cells
Proliferation
Invasion of host tissue
Toxin-induced damage to host cells
12. IMMUNE RESPONSE TO BACTERIA
The immune response to extracellular bacterial infections is generally mediated by
antibody.
Antibody can induce localized production of immune effector molecules of the
complement system.
Antibody can also activate complement-mediated lysis of the bacterium, neutralize toxins,
and serve as an opsonin to increase phagocytosis.
Some bacteria secrete protease enzymes that cleave IgA dimers, thus reducing the
effectiveness of IgA in the mucous secretions.
Host defense against intracellular bacteria depends largely on CD4 T-cell-mediated
responses.
14. PROTOZOAN DISEASES
Protozoans are unicellular eukaryotic organisms.
They are responsible for several serious diseases in humans, including ambeoblasts,
African sleeping sickness, malaria and toxoplasmois.
The type of immune response that develops to protozoan infection depend in part on the
location of the parasite within the host.
Any protozoans have life cycle stages in which they are free in the bloodstream, during
these stages the humoral antibody is most effective..
Many of these same pathogens are also capable of intracellular growth; during these
stages, cell-mediated immune reactions are effective in host defense.
15. LIFE CYCLE OF PLASMODIUM
Sporozoites enter the Bloodstream when an infected mosquito takes a blood
meal.
The Sporozoites migrate to the liver, where they multiply, transforming
liver hepatocytes into giant multinucleate schizonts, which release thousands
of merozoites into the bloodstream.
The merozoites infect red blood cells, which eventually rupture, releasing
more merozoites.
Eventually some of the merozoites differentiate into male and female
gametocytes, which are ingested by a mosquito and differentiate into
gametes.
The gametes fuse to form a zygote that differentiates to the sporozoite stage
within the salivary gland of the mosquito.
16. IMMUNE RESPONSES TO PROTOZOANS
Both humoral and cell-mediated immune responses have been implicated in immunity to
protozoan infections.
Humoral antibody is effective against blood-borne stages of the protozoan life cycle.
Once protozoans have infected host cells, cell-mediated immunity is necessary.
Protozoans escape the immune response through several mechanisms.
Trypanosoma brucei are covered by a glycoprotein coat that is constantly changed by a
genetic switch mechanism.
Plasmodium, the causative agent of malaria, slough off their glycoprotein coat after
antibody has bound to it.
18. DISEASE CAUSED BY PARASITIC
WORMS (HELMINTHS)
Helminths are large, multicellular organisms that reside in humans but do not ordinarily
multiply there and are not intracellular pathogens.
Although helminths are more accessible to the immune system than protozoans.
The immune system is not strongly engaged and the level of immunity generated to
helminths is often very poor.
Several helminthes are important pathogens of domestic animals and invade humans who
ingest contaminated food.
Taenia, a tapeworm of cattle
Trichinella, the roundworm of pigs
19. IMMUNE RESPONSE TO HELMINTHS
The response includes an IgE
humoral component and a
cell-mediated component
involving CD4 T cells.
C = complement
ECF = eosinophil
chemotactic factor
PAF = platelet-activating
factor
20. RESISTANCE / IMMUNE EVASION BY
MICROBES
Microorganisms have developed many means to resist or evade the immune system:
Antigenic variation
Growth in niches that are inaccessible to the host immune system
Resistance to innate immune defense
Impairment of effective T-cell antimicrobial responses by specific or nonspecific
immunosuppression.