The document discusses the identification and classification of insects in the orders Homoptera and Hymenoptera. Homoptera include insects like aphids, whiteflies, and scales that have piercing-sucking mouthparts and uniform wing texture. Hymenoptera undergo complete metamorphosis and include bees, ants, and wasps. The best approach for controlling pests from these orders is an integrated method using cultural, biological, and chemical methods as needed.
Thrips (order Thysanoptera) are minute (mostly 1 mm long or less), slender insects with fringed wings and unique asymmetrical mouthparts. Different thrips species feed mostly on plants by puncturing and sucking up the contents, although a few are predators. Entomologists have described approximately 6,000 species.
Plant Biodiversity Enhances Bees and Other Pollinators in Agro Ecosystems.pptxacademickushal83
In summary, exploring entomology aspects related to plant biodiversity and its impact on pollinators in agro ecosystems highlights several key points. Entomologists study pollinator diversity and foraging behavior influenced by plant diversity. They also assess habitat preferences and health, examining how access to diverse floral resources impacts pollinator populations. Moreover, entomologists investigate pesticide impacts, quantify pollinator contributions to crop pollination, and explore plant diversity's role in supporting natural pest control and climate change resilience. Entomology contributes to conservation by promoting pollinator-friendly practices and educating stakeholders. Overall, these aspects provide a comprehensive understanding of the relationship between plant biodiversity and pollinators, informing sustainable agriculture and biodiversity conservation efforts.
Thrips (order Thysanoptera) are minute (mostly 1 mm long or less), slender insects with fringed wings and unique asymmetrical mouthparts. Different thrips species feed mostly on plants by puncturing and sucking up the contents, although a few are predators. Entomologists have described approximately 6,000 species.
Plant Biodiversity Enhances Bees and Other Pollinators in Agro Ecosystems.pptxacademickushal83
In summary, exploring entomology aspects related to plant biodiversity and its impact on pollinators in agro ecosystems highlights several key points. Entomologists study pollinator diversity and foraging behavior influenced by plant diversity. They also assess habitat preferences and health, examining how access to diverse floral resources impacts pollinator populations. Moreover, entomologists investigate pesticide impacts, quantify pollinator contributions to crop pollination, and explore plant diversity's role in supporting natural pest control and climate change resilience. Entomology contributes to conservation by promoting pollinator-friendly practices and educating stakeholders. Overall, these aspects provide a comprehensive understanding of the relationship between plant biodiversity and pollinators, informing sustainable agriculture and biodiversity conservation efforts.
Protected cultivation and pest managementKishore S. M
Protected cultivation becomes new sensation for growing crops and vegetables so management of pests is one of the major issue into consideration. This presentation gives a recent strategies of pest management.
Here i would like to inform you about different bio control agents and their mode of action, it may help you in further understanding...........................................
1. GENERAL ACCOUNT ON CLASSIFICATION, INSECT CLASSIFICATION
2. The branch of zoology that is concerned with the study of insects is called entomology. What is Applied Entomology? The branch of entomology which deals with the study of ways to control insects (Pests) in terrestrial ecosystem. Entomologist: An entomologist is a scientist who studies insects. Entomologists have many important jobs, such as the study of the classification, life cycle, distribution, physiology, behavior, ecology and population dynamics of insects. What is Entomology?
3. Body divided into 3 parts (head, thorax, and abdomen) Head with 1 pair of antennae and compound eye Mouthparts consisting of a pair of mandibles and 2 pairs of maxillae Thorax with 3 pairs of legs and usually 1 or 2 pairs of wings Abdomen is segmented Genital opening is present near anus Metamorphosis usually occurs About 1,000,000 species occur in worldwide distribution. Class Insecta
4. Class Insecta
5. The class Insecta is divided into orders on the basis of the structure of the head, including eyes, mouthparts, and antennae; the thorax, including legs and wings; and the abdomen, including segmentation. The genitalia and their accessory structures, which are usually located in the ninth abdominal segment, are also important in classification. Other taxonomic criteria include sensory receptors, pattern of wing venation, and position of mouthparts. In addition, type of metamorphosis and form of larva and pupa are also used to distinguish insects. How Insects are Classified?
6. Scientific classification Kingdom: Animalia Phylum: Arthropoda Class: Insecta Order: Diptera Family: Culicidae Subfamily: Anophelinae Genus: Anopheles Species: stephensi Anopheles stephensi (Mosquito)
7. Insects are classified into 29 orders. These insect orders are the key in identifying and understanding insects. The insect orders are arranged from the most primitive wingless insects to the insect groups that have undergone the greatest evolutionary change. Most insect order names end in ptera, which comes from the Greek word pteron, meaning wing. Insects Classification
8. Insects Orders 1. Thysanura 8. Grylloblatodea 15. Zoraptera 22. Mecoptera 2. Diplura 9. Orthoptera 16. Psocoptera 23. Siphonaptera 3. Protura 10. Phasmida 17. Mallophaga 24. Coleoptera 4. Collembola 11. Dermaptera 18. Siphunculata 25. Strepsiptera 5. Ephemeroptera 12. Embiidina 19. Hemiptera 26. Diptera 6. Odonata 13. Dictyoptera 20. Thysanoptera 27. Lepidoptera 7. Plecoptera 14. Isoptera 21. Neuroptera 28. Trichoptera 29. Hymenoptera
HCM 124 lecture 9 medical entomology.pptxelphaswalela
I. OBJECTIVES
1
•To demonstrate good aseptic technique in culture transfer or inoculation and in
handling sterile materials.
•To demonstrate skil in isolation of organisms from a mixed culture using selectve
and differential media.
•To isoate microorganisms from a wide variety of sources and describe their colonial
morphology.
II. INTRODUCTION
Most environments carry a mixed microbial population. To fully appreciate the
contribution of each group of organisms to the ecology of the mass, one must first
dissect this mixed culture to obtain single colonies. The single colony is transferred
(picked) to a fresh medium to obtain a larger, homogeneous culture that may be studied
and characterized by a variety of techniques. One suchtechnique is caled aseptic
technique. Microbiologists and health workers use this technique to prevent
contamination of cultures from outside sources and to prevent the introduction of
potential disease agents into the human body (nfection can occur through contamination
of your hands and clothing with material from your bacterial cultures).
Aseptic Techniques
Aseptic techniques (also called sterile techniques) are defined as the processes
required for transferring a culture from one vessel to anotherwithout introducing any
additional organisms to the cuture or contaminating the environment with the culture.
The following conditions must exist for aseptic technique to be successful:
1. The work area must be wiped with an antiseptic to reduce the number of potential
contaminants.
2. The transfer instruments must be sterile.
3. The work must be accomplished quicky and efficiently to minimize the time of
exposure during which contamnation of the culture or laboratory worker can occur.
Developing a thorough understanding and knowledge of aseptic techniques and
culture transfer procedures is a prerequisite to working with microbiological cutures. You
wll save yourself a lot of time and energy and avoid erroneous results if a few simple
and common sense rules are observed when working with cultures.
Dr. Eby Bassiri ebassiri@sas.upenn.edu
you can find answers to what are insects, evolution, why are they dominant creatures on earth, population size, where they are found, life cycle, how insects see, etc.,
This presentation was delivered by Dr. Majumdar to over 40 hoop house (organic) farmers at a meeting organized by the Federation of Southern Cooperatives (Epes, AL). Future workshops will have more indepth information about insects lifecycles and organic control methods suitable for hoop house agriculture.
The non-biting flies are one of the most important pest group because they are not only nuisance pests, but many are also important in disease transmission.
2) Flies feed on a variety of food materials but most of those of structural importance develop in either fermenting or decaying organic matter, although a few are parasitic on other animals. Fly control is often quite challenging because their larval developmental sites must usually be located and eliminated for success, and these sites may be some distance from where the adults are nuisance. Also, flies may invade the walls and attics of structures in large numbers to overwinter, presenting winter-time control challenge.
This presentation was delivered at five vegetable production meetings across Alabama and it consisted of various sub-topics such as new insecticides, trap cropping, pheromone-based monitoring systems, and insect identification and scouting techniques.
MICROBIAL INSECTIDES...USES AND APPLICATIONS OF INSECTICIDESlokismicrobiology
Microbial insecticides are those microorganisms or their products that are capable of attacking and killing pest insects. This may be considered to be an aspect of biological control, but when one is dealing with the products of such microbes the control may be termed chemical.
This presentation was given on October 10, 2013, in Hartselle, AL. Has some news slides about factors that influence insect pests in high tunnels and some slides on how to improve quality of organic produce with correct use of organic insecticides. Refer to the other long version of the high tunnel IPM presentation I have for details about the three-tiered IPM recommendations for Alabama producers.
In statistics, the two-way analysis of variance is an extension of the one-way ANOVA that examines the influence of two different categorical independent variables on one continuous dependent variable.
Weed biology is the study of the establishment, growth, reproduction, and life cycles of weed species and weed societies/vegetation. Weed biology is an integrated science with the aim of minimizing the negative effects, as well as using and developing the positive effects, of weeds.
Protected cultivation and pest managementKishore S. M
Protected cultivation becomes new sensation for growing crops and vegetables so management of pests is one of the major issue into consideration. This presentation gives a recent strategies of pest management.
Here i would like to inform you about different bio control agents and their mode of action, it may help you in further understanding...........................................
1. GENERAL ACCOUNT ON CLASSIFICATION, INSECT CLASSIFICATION
2. The branch of zoology that is concerned with the study of insects is called entomology. What is Applied Entomology? The branch of entomology which deals with the study of ways to control insects (Pests) in terrestrial ecosystem. Entomologist: An entomologist is a scientist who studies insects. Entomologists have many important jobs, such as the study of the classification, life cycle, distribution, physiology, behavior, ecology and population dynamics of insects. What is Entomology?
3. Body divided into 3 parts (head, thorax, and abdomen) Head with 1 pair of antennae and compound eye Mouthparts consisting of a pair of mandibles and 2 pairs of maxillae Thorax with 3 pairs of legs and usually 1 or 2 pairs of wings Abdomen is segmented Genital opening is present near anus Metamorphosis usually occurs About 1,000,000 species occur in worldwide distribution. Class Insecta
4. Class Insecta
5. The class Insecta is divided into orders on the basis of the structure of the head, including eyes, mouthparts, and antennae; the thorax, including legs and wings; and the abdomen, including segmentation. The genitalia and their accessory structures, which are usually located in the ninth abdominal segment, are also important in classification. Other taxonomic criteria include sensory receptors, pattern of wing venation, and position of mouthparts. In addition, type of metamorphosis and form of larva and pupa are also used to distinguish insects. How Insects are Classified?
6. Scientific classification Kingdom: Animalia Phylum: Arthropoda Class: Insecta Order: Diptera Family: Culicidae Subfamily: Anophelinae Genus: Anopheles Species: stephensi Anopheles stephensi (Mosquito)
7. Insects are classified into 29 orders. These insect orders are the key in identifying and understanding insects. The insect orders are arranged from the most primitive wingless insects to the insect groups that have undergone the greatest evolutionary change. Most insect order names end in ptera, which comes from the Greek word pteron, meaning wing. Insects Classification
8. Insects Orders 1. Thysanura 8. Grylloblatodea 15. Zoraptera 22. Mecoptera 2. Diplura 9. Orthoptera 16. Psocoptera 23. Siphonaptera 3. Protura 10. Phasmida 17. Mallophaga 24. Coleoptera 4. Collembola 11. Dermaptera 18. Siphunculata 25. Strepsiptera 5. Ephemeroptera 12. Embiidina 19. Hemiptera 26. Diptera 6. Odonata 13. Dictyoptera 20. Thysanoptera 27. Lepidoptera 7. Plecoptera 14. Isoptera 21. Neuroptera 28. Trichoptera 29. Hymenoptera
HCM 124 lecture 9 medical entomology.pptxelphaswalela
I. OBJECTIVES
1
•To demonstrate good aseptic technique in culture transfer or inoculation and in
handling sterile materials.
•To demonstrate skil in isolation of organisms from a mixed culture using selectve
and differential media.
•To isoate microorganisms from a wide variety of sources and describe their colonial
morphology.
II. INTRODUCTION
Most environments carry a mixed microbial population. To fully appreciate the
contribution of each group of organisms to the ecology of the mass, one must first
dissect this mixed culture to obtain single colonies. The single colony is transferred
(picked) to a fresh medium to obtain a larger, homogeneous culture that may be studied
and characterized by a variety of techniques. One suchtechnique is caled aseptic
technique. Microbiologists and health workers use this technique to prevent
contamination of cultures from outside sources and to prevent the introduction of
potential disease agents into the human body (nfection can occur through contamination
of your hands and clothing with material from your bacterial cultures).
Aseptic Techniques
Aseptic techniques (also called sterile techniques) are defined as the processes
required for transferring a culture from one vessel to anotherwithout introducing any
additional organisms to the cuture or contaminating the environment with the culture.
The following conditions must exist for aseptic technique to be successful:
1. The work area must be wiped with an antiseptic to reduce the number of potential
contaminants.
2. The transfer instruments must be sterile.
3. The work must be accomplished quicky and efficiently to minimize the time of
exposure during which contamnation of the culture or laboratory worker can occur.
Developing a thorough understanding and knowledge of aseptic techniques and
culture transfer procedures is a prerequisite to working with microbiological cutures. You
wll save yourself a lot of time and energy and avoid erroneous results if a few simple
and common sense rules are observed when working with cultures.
Dr. Eby Bassiri ebassiri@sas.upenn.edu
you can find answers to what are insects, evolution, why are they dominant creatures on earth, population size, where they are found, life cycle, how insects see, etc.,
This presentation was delivered by Dr. Majumdar to over 40 hoop house (organic) farmers at a meeting organized by the Federation of Southern Cooperatives (Epes, AL). Future workshops will have more indepth information about insects lifecycles and organic control methods suitable for hoop house agriculture.
The non-biting flies are one of the most important pest group because they are not only nuisance pests, but many are also important in disease transmission.
2) Flies feed on a variety of food materials but most of those of structural importance develop in either fermenting or decaying organic matter, although a few are parasitic on other animals. Fly control is often quite challenging because their larval developmental sites must usually be located and eliminated for success, and these sites may be some distance from where the adults are nuisance. Also, flies may invade the walls and attics of structures in large numbers to overwinter, presenting winter-time control challenge.
This presentation was delivered at five vegetable production meetings across Alabama and it consisted of various sub-topics such as new insecticides, trap cropping, pheromone-based monitoring systems, and insect identification and scouting techniques.
MICROBIAL INSECTIDES...USES AND APPLICATIONS OF INSECTICIDESlokismicrobiology
Microbial insecticides are those microorganisms or their products that are capable of attacking and killing pest insects. This may be considered to be an aspect of biological control, but when one is dealing with the products of such microbes the control may be termed chemical.
This presentation was given on October 10, 2013, in Hartselle, AL. Has some news slides about factors that influence insect pests in high tunnels and some slides on how to improve quality of organic produce with correct use of organic insecticides. Refer to the other long version of the high tunnel IPM presentation I have for details about the three-tiered IPM recommendations for Alabama producers.
In statistics, the two-way analysis of variance is an extension of the one-way ANOVA that examines the influence of two different categorical independent variables on one continuous dependent variable.
Weed biology is the study of the establishment, growth, reproduction, and life cycles of weed species and weed societies/vegetation. Weed biology is an integrated science with the aim of minimizing the negative effects, as well as using and developing the positive effects, of weeds.
Primary salinity is caused by natural processes such the accumulation of salt from rainfall over many thousands of years or from the weathering of rocks.
The study revealed that the range for the pH values was recorded between 6.8 and 8.5 mg/L. Average Electrical Conductivity (EC) was recorded between 103 µS/cm – 33016 µS/cm and average Salinity was recorded between recorded 0.1ppt-31.40ppt during the sampling period.
The current nitrate levels in drinking water in Jaffna peninsula are much higher than the WHO and SLS levels. The nitrate-N content of groundwater in the Jaffna Peninsula ranges from 0.1 to 45 mg/L as per the literature though the permissible nitrate-N level in drinking water is 10 mg/L.
Phosphorus (P), next to nitrogen, is often the most limiting nutrient for crop and forage production. Phosphorus' primary role in a plant is to store and transfer energy produced by photosynthesis for use in growth and reproductive processes. Soil P cycles in a variety forms in the soil
Paddy fields account for around 20% of human-related emissions of methane — a potent greenhouse gas. Farmers normally flood rice fields throughout the growing season, meaning that methane is produced by microbes underwater as they help to decay any flooded organic matter
Routine maintenance involves complete replacement of equipment based on time intervals without any inspections. This is also a common maintenance strategy applied to industrial equipment, for example, for the replacement of filters and wearing components
A rose is a woody perennial flowering plant of the genus Rosa, in the family Rosaceae, or the flower it bears. There are over three hundred species and tens of thousands of cultivars. They form a group of plants that can be erect shrubs, climbing, or trailing, with stems that are often armed with sharp prickles.
There are two main types of hydraulic power transmission systems: hydrokinetic, such as the hydraulic coupling and the hydraulic torque converter, which use the kinetic energy of the liquid; and hydrostatic, which use the pressure energy of the liquid. The hydraulic coupling is a device that links two rotatable shafts.
Nursery management may be defined as the sum of the activities performed for the successful production, care, and marketing of different planting materials (seeds, seedlings, cuttings, etc.) in a different nursery section. Conducting employees properly, maintenance care and protection of properties, etc
Current estimates of soil C storage potential are based on models or factors that assume linearity between C input levels and C stocks at steady-state, implying that SOC stocks could increase without limit as C input levels increase. However, some soils show little or no increase in steady-state SOC stock with increasing C input levels suggesting that SOC can become saturated with respect to C input.
Artificial insemination is the deliberate introduction of sperm into a female's cervix or uterine cavity for the purpose of achieving a pregnancy through in vivo fertilization by means other than sexual intercourse or in vitro fertilisation.
Chemical Industries (Colombo) Limited was incorporated in 1964 as a supplier of high-quality chemical products for the local market.
CIC acquired the Pelwehera Farm in the year 2000 under the prevailing government’s privatization programme.
symbiotic N fixation & challenges to extension to NSupun Madushanka
Some nitrogen-fixing bacteria have symbiotic relationships with [[plant groups, especially legumes. Looser non-symbiotic relationships between diazotrophs and plants are often referred to as associative, as seen in nitrogen fixation on rice roots. Nitrogen fixation occurs between some termites and fungi.
The diesel engine was invented during the industrial revolution by a German engineer. Rudolf Diesel grew up in France but then left for England during the Franco-German war. ... By studying thermodynamics, Diesel found he could make a smaller, internal combustion engine that would convert all heat into work.
A combination of biotechnology and nanotechnology has the potential to revolutionize agricultural systems and provide solutions for current and future problems. These include the development and use of smart fertilizers with controlled nutrient release, together with bioformulations based on bacteria or enzymes.
Participatory methods in Agricultural TechnologySupun Madushanka
Participatory technology development (PTD) is an approach to learning and innovation that is used in international development as part of projects and programmes relating to sustainable agriculture.
The irrigation works in ancient Sri Lanka, the earliest dating from about 300 BCE, in the reign of King Pandukabhaya and under continuous development for the next thousand years, were some of the most complex irrigation systems of the ancient world.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
2. CONTENT
Objective
Identification of insect
Major characteristics and their orders
Identification of homoptera and ultimately their controlling
method
Getting an idea about Hymenoptera and controlling method
Summary
References
2
3. OBJECTIVE
3
To identify insects their major characteristics and their
orders
Having a good understand about Homopterans and
Hymenopterans
4. IDENTIFICATION OF INSECTS
An insect is a very small animal with particular characteristics. Insects
are invertebrates. They have no backbones. Most insects walk, but
some can fly and jump.
Domain - Eukarya
Kingdom - Animalia
Phylum - Arthropod
Class - Insecta
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5. MAJOR CHARACTERISTICS
All Insects have an exoskeleton.
They have three main body parts: THORAX , ABDOMEN , AND HEAD.
Insects also have a pair of antennae on the top of their heads
Insects have compound eyes, three pairs of legs and many have two pairs of
wings,
Abdomen – the posterior (farthest to the rear) section of an insect’s body
Antennae – little feelers on top of the insect’s head that help them receive
messages, sound, and motion
Thorax – the but not all Abdomen – the posterior (farthest to the rear) section of
an insect’s body middle section of an insect’s body
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9. HOMOPTERA SUBORDERS
Auchenorrhyncha
Red-banded leafhopper, Graphocephala coccinea
Scientific classification
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Hemiptera
Suborder: Auchenorrhyncha
Infraorders
•Cicadomorpha
•Fulgoromorpha
An aphid.
Scientific classification
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Hemiptera
Suborder: Sternorrhyn
ha
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10. STERNORRHYNCHA
The aphids and scale insects are the other well-known "Homoptera“.
All members of this group are plant-feeders, and many are vectors of viral and
fungal diseases of plants.
Auchenorrhyncha
Auchenorrhyncha species produce either audible sounds or substrate
vibrations as a form of communication.
Such calls range from vibrations inaudible to humans, to the calls of many
species of cicadas that can be heard for hundreds of meters at least.
In season, they produce the most characteristic and ubiquitous noise of the
bush. The Auchenorrhyncha (Cicadinea) cicadas, leafhoppers, treehoppers,
and spittlebugs.
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11. ORDINAL CHARACTERISTICS
Piercing-sucking mouthparts arising from posterior margin of the head
front wings
Homogeneous texture Wings held roof-like over abdomen
Phytophagous.
Gradual metamorphosis.
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12. Leaf hoppers
Morphological characteristics
Leafhopper adults and nymphs are active.
jumping insects recognized by their piercing-
sucking mouthparts and by the presence of
four rows of enlarged, spine like setae on their
hind tibiae.
Damage
Light colored speckling on plant leaves
sucking sap and plant juices from within the
plant tissues.
Leaf unchecked, this gradual feeding reduces
the plant’s vigor over time, browning the
leaves.
Control
covering all leaves and stems with insecticidal
soap to control nymphs.
Add 1 tablespoon of rubbing alcohol to 1
quart of soap to boost the soap’s effectiveness.
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13. Cicadas – Homoptera
Wings – 2 and straight if present
Mouthparts – piercing and sucking
Metamorphosis – incomplete
• Annual cicadas typically cause only minimal,
non-permanent damage to mature trees.
• such as tip browning and some leaf wilting
due to the laying of eggs in the branch tips.
Damage
Controlling method
Non-Chemical Cicada Control
• On small or young trees, wrap the
branches loosely with cheesecloth or other
mesh cloth.
• Knock the insects off of small trees and
shrubs with blasts of water from a garden
hose.
• Do not plant trees within a year or two
prior to the predicted emergence of
periodical cicadas in that area.
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15. Physical Methods
Barriers
Water barriers
Fences
Nets
Hand picking
Shaking plants
Fire
Temperature Control
Traps
Light traps
Sticky traps
Electromagnetic energy
Use of Visible (0.38-0.78µ) and
UV radiation (2*10^-2 – 0.38µ)
Use of radio frequency
Advantages Disadvantages
Some methods are inexpensive High technical methods
are expensive
Less hazardous to health &
environment
Not always practical
Have immediate effect
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16. Chemical Methods
Neem insecticidal oil spray
Systemic insecticides
usually contain neonicotinoid compounds
as active ingredients
Advantages Disadvantages
High efficiency detrimental for the
environment and
produce considerable
damage to ecosystems
act very fast may be harmful for non-
target species.
pollute air, water and soil
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17. Biological Control
Predators
Parasitoids
Pathogen
Competitors
Advantages Disadvantages
Highly specific to one pest. Only a few working examples
A long term solution if
equilibrium is established.
Expensive to research and a high
level of skills and initial set up
costs.
Inexpensive over the long term. Agent may become a pest itself.
No environmental Frequent input needed to
maintain population balance.
Can be used in a glasshouse. Needs to be large scale
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18. Cultural Methods
• Use of resistant varieties
• Crop rotation
• Crop residues destruction
• Tilling and cultivating the soil
• Pruning and thinning
• Fertilization
• Clean culture
• Water management
• Planting of trap crops
• Use of clean seed
• Variation in time of plant and harvesting
Advantages Disadvantages
no extra cost is required. No visible results
observe
safe for application. not effective at
epidemic condition
Effective for many pests
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21. Metamorphosis
Hymenoptera are Holometabolous, their life cycle consists of four
phases egg, multiple instars, pupa, and adult.
"Complete metamorphosis" is the common term for this life cycle.
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BEE ANT
29. SUMMERY
Insects devide into more groups and among them,homoptera has uniform texture
of the front wing and hymonoptera has membranous nature of wins.
Homoptera are sucking insects and hymonoptera are chewing insects.
Both of them have complete life cycles .Nymph stage of homoptera and larve stage
of hymonoptera are the destructive stages.
Examples for the homopterans are aphids and bees ,ants, true wasps, sawfly
classified under hymenopterans.
The best method is integrated method for controlling those pests. Exept that,
can use pest cultural practices, biological methods and insecticides at the
economic injury level.