Microbial biomass conversion processes take advantage of the ability of microorganisms to consume and digest biomass and release hydrogen. Depending on the pathway, this research could result in commercial-scale systems in the mid- to long-term timeframe that could be suitable for distributed, semi-central, or central hydrogen production scales, depending on the feedstock used.
Biohydrogen may produced by steam reforming of methane (biogas) produced by anaerobic digestion of organic waste. In the latter process, natural gas and steam react to produce hydrogen and carbon dioxide.
Hydrocarbon are major constituents of crude oil and petroleum. They can be biodegraded by naturally-occurring microorganisms in freshwater and marine environments under a variety of aerobic and anaerobic conditions. The ability of microorganisms - bacteria, archaea, fungi, or algae - to break down hydrocarbons is the basis for natural and enhanced bioremediation. To promote biodegradation, amendments such as nitrogen and phosphorous fertilizer are often added to stimulate microbial growth and metabolism
Methanogenesis or biomethanation is the formation of methane by microbes known as methanogens. Organisms capable of producing methane have been identified only from the domain Archaea, a group phylogenetically distinct from both eukaryotes and bacteria, although many live in close association with anaerobic bacteria.
Biohydrogen may produced by steam reforming of methane (biogas) produced by anaerobic digestion of organic waste. In the latter process, natural gas and steam react to produce hydrogen and carbon dioxide.
Hydrocarbon are major constituents of crude oil and petroleum. They can be biodegraded by naturally-occurring microorganisms in freshwater and marine environments under a variety of aerobic and anaerobic conditions. The ability of microorganisms - bacteria, archaea, fungi, or algae - to break down hydrocarbons is the basis for natural and enhanced bioremediation. To promote biodegradation, amendments such as nitrogen and phosphorous fertilizer are often added to stimulate microbial growth and metabolism
Methanogenesis or biomethanation is the formation of methane by microbes known as methanogens. Organisms capable of producing methane have been identified only from the domain Archaea, a group phylogenetically distinct from both eukaryotes and bacteria, although many live in close association with anaerobic bacteria.
Biodegradation or biological degradation is the phenomenon of biological transformation of organic compounds by living organisms, particularly the microorganisms.
Biodegradation basically involves the conversion of complex organic molecules to simpler (and mostly non-toxic) ones. The term biotransformation is used for incomplete biodegradation of organic compounds involving one or a few reactions. Biotransformation is employed for the synthesis of commercially important products by microorganisms.
Bioremediation refers to the process of using microorganisms to remove the environmental pollutants i.e. the toxic wastes found in soil, water, air etc. The microbes serve as scavengers in bioremediation. The removal of organic wastes by microbes for environmental clean-up is the essence of bioremediation. The other names used (by some authors) for bioremediation are bio-treatment, bio-reclamation and bio-restoration.
It is rather difficult to show any distinction between biodegradation and bioremediation. Further, in biotechnology, most of the reactions of biodegradation/bioremediation involve xenobiotic.
Industrial Production of Amino Acid (L-Lysine)Mominul Islam
Three amino acids which are produced at large scale includes-
- L-lysine
- L-glutamic acid
- DL- methionine
We are now going to discuss about the production of L-Lysine
“Bioleaching" or "bio-oxidation" employs the use of naturally occurring bacteria, harmless to both humans and the environment, to extract of metals from their ores.
Conversion of insoluble metal sulfides into water-soluble metal sulfates.
It is mainly used to recover certain metals from sulfide ores. This is much cleaner than the traditional leaching.
Bioremediation of heavy metals pollution by Udaykumar Pankajkumar BhanushaliUdayBhanushali111
Mechanisms and techniques used for Bioremediation which includes phytoremediation, Bacterial & fungal bioremediation. Examples of heavy metal pollution
Hydrogen production from Biological organisms as well as from electrochemical or thermal process which is helpful for transportation.Advantage: No emission of Green House effect
Biodegradation or biological degradation is the phenomenon of biological transformation of organic compounds by living organisms, particularly the microorganisms.
Biodegradation basically involves the conversion of complex organic molecules to simpler (and mostly non-toxic) ones. The term biotransformation is used for incomplete biodegradation of organic compounds involving one or a few reactions. Biotransformation is employed for the synthesis of commercially important products by microorganisms.
Bioremediation refers to the process of using microorganisms to remove the environmental pollutants i.e. the toxic wastes found in soil, water, air etc. The microbes serve as scavengers in bioremediation. The removal of organic wastes by microbes for environmental clean-up is the essence of bioremediation. The other names used (by some authors) for bioremediation are bio-treatment, bio-reclamation and bio-restoration.
It is rather difficult to show any distinction between biodegradation and bioremediation. Further, in biotechnology, most of the reactions of biodegradation/bioremediation involve xenobiotic.
Industrial Production of Amino Acid (L-Lysine)Mominul Islam
Three amino acids which are produced at large scale includes-
- L-lysine
- L-glutamic acid
- DL- methionine
We are now going to discuss about the production of L-Lysine
“Bioleaching" or "bio-oxidation" employs the use of naturally occurring bacteria, harmless to both humans and the environment, to extract of metals from their ores.
Conversion of insoluble metal sulfides into water-soluble metal sulfates.
It is mainly used to recover certain metals from sulfide ores. This is much cleaner than the traditional leaching.
Bioremediation of heavy metals pollution by Udaykumar Pankajkumar BhanushaliUdayBhanushali111
Mechanisms and techniques used for Bioremediation which includes phytoremediation, Bacterial & fungal bioremediation. Examples of heavy metal pollution
Hydrogen production from Biological organisms as well as from electrochemical or thermal process which is helpful for transportation.Advantage: No emission of Green House effect
Electricity Generation from Biogas Produced in a Lab-Scale Anaerobic Digester...inventionjournals
The sludge produced during wastewater treatment should be stabilized in order to minimize the damage to the environment. This study includes the evaluation of sludge stabilization and biogas formation by anaerobic digestion in order to generate electricity using stirling motor.The study was carried out with the raw sludge form the thickener of the wastewatertreatment plant. The main aim of the study is to provide sludge stabilization resulting biogas production by reduction of organic matter and to generate electricity. Anaerobic digestion studies were carried out using a laboratory scale anaerobic reactor with a volume of 7L.Under themesophilic condition, the sludge age was maintained at 10 days during the first 20 days of operation, while the reactor was operated for 90 days until the end of the run, with a sludge age of 20 days.The results have changed in the range of 42-52% after the organic matter reduction obtained from the anaerobic digestion. Concentrations of 3735.7300 ppm, 5060.5768 ppm, and 6951.4013 ppm biogas were obtained. Biogas was turned on by mechanical energy with a Stirlingmotor and then turned to direct current and the lamps with 3V 20mA each were run for 60 minutes
This brief document describes how to convert waste into energy, particularly electricity. It is a new way of waste management. It is eco-friendly and helps fight climate change which has become a global crisis.
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
Extraction of Bio-Fuel from Algae by Anaerobic DigestionEditor IJMTER
The growing energy demand across the globe has instigated us to synthesize bio-fuel
from algae, a renewable resource. Algae Botryococcus braunii when subjected to anaerobic digestion
and broken down by enzymes liberate methane and CO2. The CO2 obtained is cultivated in open
ponds and are passed through a fluidised bed chamber after pre-treatment. The chamber contains
enzymes which breakdown the algal colloid into fatty acids. These fatty acids on decomposition
release CO2 that is internally cycled for algal cultivation and the methane can be profitably and
cleanly extracted. This methane can be used as a fuel in vehicles (CNG) and also in various industrial
and domestic fields, providing a low-cost solution to the global energy crisis.
Isolation and Screening of Hydrogen Producing Bacterial Strain from Sugarcane...Editor IJCATR
The aim of this study is to isolate a highly competent bacterium with potent cellulose degrading capability and a better
hydrogen producer. Soil sample from sugarcane bagasse yard was isolated, serially diluted and plated on cellulose specific nutrient
agar plate. Four colonies have been isolated in which a single colony has potent cellulose degrading ability and the highest hydrogen
productivity of 275.13 mL H2 L-1. The newly isolated bacterium was morphologically and biochemically characterized. The
molecular characterization of the bacterium was carried out using 16S rDNA sequencing and the organism was identified as
Bacilllus subtilis AuChE413. Proteomic analysis such as MALDI-TOF was carried out to differentiate the isolated Bacillus subtilis
from Bacillus thuringiensis and Bacillus amyloliquefaciens. Phylogenetic tree was constructed to analyze the evolutionary
relationship among different genus and species with the newly isolated strain.
The problems attract worldwide attention K/a Global Environmental Problems.
The top three environmental problems are: (1) Greenhouse Effect and Global Warming (2) Depletion of Ozone and (3) Acid Rain.
Aim1: To study the method of genome identification through ENSEMBL browser.
Aim2: To study the method of genome identification through VISTA.
Aim3: To study the method of genome identification through UCSC Genome Browser.
Aim4: To study the method of genome and amino acid sequences through UCSC Genome Browser.
Intracellular Components
We will now begin our discussion of intracellular organelles. As we have mentioned, only eukaryotic cells have intracellular sub-divisions, so our discussion will exclude prokaryotic cells. We will also focus on animal cells, since plant cells have a number of further specialized structures. In this section we will discuss the importance of the cell nucleus, mitochondria, peroxisomes, endoplasmic reticulum, golgi apparatus, and lysosome.
Types of Receptors
Receptors are protein molecules in the target cell or on its surface that bind ligands. There are two types of receptors: internal receptors and cell-surface receptors.
The cells derived from root apical and shoot-apical meristems and cambium differentiate and mature to perform specific functions. This act leading to maturation is termed as differentiation. During differentiation, cells undergo few to major structural changes both in their cell walls and protoplasm. The living differentiated cells, that by now have lost the capacity to divide can regain the capacity of division under certain conditions. This phenomenon is termed as dedifferentiation. For example, formation of meristems – interfascicular cambium and cork cambium from fully differentiated parenchyma cells. While doing so, such meristems / tissues are able to divide and produce cells that once again lose the capacity to divide but mature to perform specific functions, i.e., get redifferentiated.
Meat and milk from farmed animals including livestock (cattle, goat and buffalo) and poultry are sources of high quality protein and essential amino acids, minerals, fats and fatty acids, readily available vitamins, small quantities of carbohydrates and other bioactive components.1 The Food and Agriculture Organization (FAO) 2008 estimate shows that meat consumption has grown with increase in population. The average global per capita meat consumption is 42.1 kg/year with 82.9 kg/year in developed and 31.1 kg/year in developing countries in a recommended daily animal-sourced protein per capita of 50 kg per year2. Milk on the other hand is consumed in various forms: liquid, cheese, powder, and cream at a global per capita consumption of 108 kg per person per year which is way below the FAO recommended daily consumption of 200 kg.
Antibodies, also known as immunoglobulins, are secreted by B cells (plasma cells) to neutralize antigens such as bacteria and viruses. The classical representation of an antibody is a Y-shaped molecule composed of four polypeptides-two heavy chains and two light chains. Each tip of the "Y" contains a paratope (a structure analogous to a lock) that is specific for one particular epitope (similarly analogous to a key) on an antigen, allowing these two structures to bind together with precision. The ability of binding to an antigen has led to their ubiquitous use in a variety of life science and medical science. These antibodies can be classified into two primary types (monoclonal and polyclonal) by the means in which they are created from lymphocytes. Each of them has important role in the immune system, diagnostic exams, and treatments.
There are many characteristics of biological data. All these characteristics make the management of biological information a particularly challenging problem. Here mainly we will focus on characteristics of biological information and multidisciplinary field called bioinformatics. Bioinformatics, now a days has emerged with graduate degree programs in several universities.
Hormones, Proteins, etc. present in blood in minute concentration can be assayed by the recent advanced technique of “Enzyme Immuno Assay” without involving any disadvantage. The basic reaction is the interaction between an antibody and an antigen.
Meat and milk from farmed animals including livestock (cattle, goat and buffalo) and poultry are sources of high quality protein and essential amino acids, minerals, fats and fatty acids, readily available vitamins, small quantities of carbohydrates and other bioactive components.1 The Food and Agriculture Organization (FAO) 2008 estimate shows that meat consumption has grown with increase in population. The average global per capita meat consumption is 42.1 kg/year with 82.9 kg/year in developed and 31.1 kg/year in developing countries in a recommended daily animal-sourced protein per capita of 50 kg per year2. Milk on the other hand is consumed in various forms: liquid, cheese, powder, and cream at a global per capita consumption of 108 kg per person per year which is way below the FAO recommended daily consumption of 200 kg.
In shotgun sequencing the genome is broken randomly into short fragments (1 to 2 kbp long) suitable for sequencing. The fragments are ligated into a suitable vector and then partially sequenced. Around 400–500 bp of sequence can be generated from each fragment in a single sequencing run. In some cases, both ends of a fragment are sequenced. Computerized searching for overlaps between individual sequences then assembles the complete sequence.
Sequence assembly refers to aligning and merging fragments from a longer DNA sequence in order to reconstruct the original sequence. This is needed as DNA sequencing technology cannot read whole genomes in one go, but rather reads small pieces of between 20 and 30,000 bases, depending on the technology used. Typically the short fragments, called reads, result from shotgun sequencing genomic DNA, or gene transcript (ESTs).
The problem of sequence assembly can be compared to taking many copies of a book, passing each of them through a shredder with a different cutter, and piecing the text of the book back together just by looking at the shredded pieces. Besides the obvious difficulty of this task, there are some extra practical issues: the original may have many repeated paragraphs, and some shreds may be modified during shredding to have typos. Excerpts from another book may also be added in, and some shreds may be completely unrecognizable.
Vaccine (L. vacca = cow) is a preparation/suspension or extract of dead/attenuated (weakened) germs of a disease which on inoculation (injection) into a healthy person provides temporary/permanent active/passive immunity by inducing antibodies formation.
Thus antibody provoking agents are called vaccines.
Biological treatment is an important and integral part of any wastewater treatment plant that treats wastewater from either municipality or industry having soluble organic impurities or a mix of the two types of wastewater sources.
The four processes are: (1) Preliminary Treatment (2) Primary Treatment (3) Secondary or Biological Treatment and (4) Tertiary or Advanced Treatment
The genetic variations found in the in vitro cultured cells are collectively referred to as somaclonal variations.
The plants derived from such cells are referred to somaclones. Some authors use the terms calliclones and proto-clones to represent cultures obtained from callus and protoplasts respectively.
The growth of plant cells in vitro is an asexual process involving only mitotic division of cells. Thus, culturing of cells is the method to clone a particular genotype. It is therefore expected that plants arising from a given tissue culture should be the exact copies of the parental plant.
The occurrence of phenotypic variants among the regenerated plants (from tissue cultures) has been known for several years. These variations were earlier dismissed as tissue culture artefacts. The term somaclonal variations was first used by Larkin and Scowcraft (1981) for variations arising due to culture of cells, i.e., variability generated by a tissue culture. This term is now universally accepted.
As described elsewhere the explant used in tissue culture may come from any part of the plant organs or cells. These include leaves, roots, protoplasts, microspores and embryos. Somaclonal variations are reported in all types of plant tissue cultures.
In recent years, the term gametoclonal variations is used for the variations observed in the regenerated plants from gametic cells (e.g., anther cultures). For the plants obtained from protoplast cultures, proto-clonal variations is used.
Solid waste management is a polite term for garbage management. As long as humans have been living in settled communities, solid waste, or garbage, has been an issue, and modern societies generate far more solid waste than early humans ever did.
The chemical compounds produced by plants are collectively referred to as phytochemicals. Biotechnologists have special interest in plant tissue culture for the large scale production of commercially important compounds. These include pharmaceuticals, flavours, fragrances, cosmetics, food additives, feed stocks and antimicrobials.
Most of these products are secondary metabolites— chemical compounds that do not participate in metabolism of plants. Thus, secondary metabolites are not directly needed by plants as they do not perform any physiological function (as is the case with primary metabolites such as amino acids, nucleic acids etc.). Although the native plants are capable of producing the secondary metabolites of commercial interest, tissue culture systems are preferred.
The biotic stresses are caused by insects, pathogens (viruses, fungi, bacteria), and wounds. The abiotic stresses are due to herbicides, water deficiency (caused by drought, temperature, and salinity), ozone and intense light.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
1. Hydrogen Production: Microbial Biomass Conversion
Dr. Naveen Gaurav
Associate Professor and Head
Department of Biotechnology
Shri Guru Ram Rai University
Dehradun
2. Hydrogen Production: Microbial Biomass Conversion
Microbial biomass conversion processes take advantage of the ability of microorganisms to
consume and digest biomass and release hydrogen. Depending on the pathway, this
research could result in commercial-scale systems in the mid- to long-term timeframe that
could be suitable for distributed, semi-central, or central hydrogen production scales,
depending on the feedstock used.
HOW DOES IT WORK?
In fermentation-based systems, microorganisms, such as bacteria, break down organic
matter to produce hydrogen. The organic matter can be refined sugars, raw biomass
sources such as corn stover, and even wastewater. Because no light is required, these
methods are sometimes called "dark fermentation" methods.
In direct hydrogen fermentation, the microbes produce the hydrogen themselves. These
microbes can break down complex molecules through many different pathways, and the
byproducts of some of the pathways can be combined by enzymes to produce hydrogen.
Researchers are studying how to make fermentation systems produce hydrogen faster
(improving the rate) and produce more hydrogen from the same amount of organic matter
(increasing the yield).
Microbial electrolysis cells (MECs) are devices that harness the energy and protons
produced by microbes breaking down organic matter, combined with an additional small
electric current, to produce hydrogen. This technology is very new, and researchers are
working on improving many aspects of the system, from finding lower-cost materials to
identifying the most effective type of microbes to use.
3. WHY IS THIS PATHWAY BEING CONSIDERED?
Biomass is an abundant domestic resource, and many microbes have evolved to efficiently
break down biomass to produce hydrogen and other products. Fermentation has already
been used as an industrial technology to generate biofuels and other products, and many
of the challenges to scaling up systems have been addressed for different products,
allowing hydrogen researchers to focus on the challenges unique to hydrogen production.
MEC-based systems have the potential to produce hydrogen from resources that otherwise
can’t be used for fuel production, and could reduce the large amount of energy normally
needed for wastewater treatment while producing a valuable fuel in the form of hydrogen.
These two pathways can be combined to maximize the hydrogen yield from the starting
biomass feedstock.
4. Process: Hydrogen production by microbes
hydrogen-producing bacteria and algae: Clostridium, Enterobacter,
Klebsiella, Citrobacter, Bacillus and Cyanobacteria, Anabaena
5. RESEARCH FOCUSES ON OVERCOMING CHALLENGES
1. Improving the rates and yields of hydrogen production from fermentation processes
through a number of methods such as microbial strain improvements, reactor system
optimization, and identifying feedstock sources and processing methods with the highest
yields.
2. Developing MEC systems that can be scaled up to commercially relevant sizes while
maintaining the production rates and system efficiencies seen at the bench scale and
minimizing the costs of the reactor components.
Examples:
6. Nitrogenase-dependent hydrogen production
Benemann and Weare demonstrated that a nitrogen-fixing cyanobacterium, Anabaena
cylindrica, produced hydrogen and oxygen gas simultaneously in an argon atmosphere for
several hours. Nitrogenase is responsible for nitrogen-fixation and is distributed mainly
among prokaryotes, including cyanobacteria, but does not occur in eukaryotes, under which
microalgae are classified. Molecular nitrogen is reduced to ammonium with consumption of
reducing power (e' mediated by ferredoxin) and ATP. The reaction is substantially irreversible
and produces ammonia:
N2 + 6H1+ + 6e- =2HN3
12ATP ↔ 12(ADP+Pi)
However, nitrogenase catalyzes proton reduction in the absence of nitrogen gas (i.e. in an
argon atmosphere).
2H+ + 2e- = H2
4ATP ↔ 4(ADP+Pi)
Hydrogen production catalyzed by nitrogenase occurs as a side reaction at a rate of one-third
to one-fourth that of nitrogen-fixation, even in a 100% nitrogen gas atmosphere.
Nitrogenase itself is extremely oxygen-labile. Unlike in the case of hydrogenase, however,
cyanobacteria have developed mechanisms for protecting nitrogenase from oxygen gas and
supplying it with energy (ATP) and reducing power. The most successful mechanism is the
localization of nitrogenase in the heterocysts of filamentous cyanobacteria Vegetative cells
(ordinary cells) in filamentous cyanobacteria carry out oxygenic photosynthesis. Organic
compounds produced by CO; reduction are transferred into heterocysts and are decomposed to
provide nitrogenase with reducing power. ATP can be provided by PSI-dependent and
anoxygenic photosynthesis within heterocysts.
7. Investigations into prolongation and optimization of hydrogen production, revealed that
the hydrogen-producing activity of cyanobacteria was stimulated by nitrogen starvation.
The presence and physiological roles of hydrogenases in nitrogen-fixing cyanobacteria
remains controversial, but 'uptake' hydrogenase appears to consume and re-use hydrogen
gas, resulting in a decrease in net hydrogen production. Asada and Kawamura reported
aerobic hydrogen production by a nitrogen-fixing Anabaena sp., believed to be an uptake
hydrogenase-deficient strain. After being cultured for 12 days, the strain accumulated
approximately 10% hydrogen and 70% oxygen gas within the gas phase of the vessel, by the
nitrogenase side reaction, even in the presence of air.
Miyamoto et al. conducted outdoor experiments on hydrogen production by Anabaena
cylindrica, in California. A nitrogen-starved culture of the cyanobacteria was continuously
sparged by argon-based gas, while the hydrogen content of the effluent gas was measured.
The average conversion efficiency over a period of one month (combustion energy of
hydrogen gas produced by cyanobacteria/incident solar energy into the photo-bioreactor
area) was approximately 0.2%.
Mitsui and co-workers extensively screened cyanobacteria for their hydrogen-producing
ability, and tested Miami BG-7, one of the most potent hydrogen-producing cyanobacteria,
in an outdoor culture. These workers also isolated a unicellular aerobic nitrogen
fixer, Synechococcus sp. Miami BG043511, and with the use of synchronous culture
techniques, discovered a new mechanism for protecting and driving oxygen-labile
nitrogenase in non-heterocystous and oxygen-evolving cells. This strain is also a potent
hydrogen-producer, having an estimated conversion efficiency of 3.5% based on PAR using
an artificial light source.
8. Hydrogen production by photosynthetic bacteria
Photosynthetic bacteria undergo anoxygenic photosynthesis with organic compounds
or reduced sulfur compounds as electron donors. Some non-sulfur photosynthetic
bacteria are potent hydrogen producers, utilizing organic acids such as lactic, succinic
and butyric acids, or alcohols as electron donors. Since light energy is not required for
water oxidation, the efficiency of light energy conversion to hydrogen gas by
photosynthetic bacteria, is in principle much higher than that by cyanobacteria.
Hydrogen production by photosynthetic bacteria is mediated by nitrogenase activity,
although hydrogenases may be active for both hydrogen production and hydrogen
uptake under some conditions. Miyake and Kawamura demonstrated a maximum
energy conversion efficiency (combustion energy of hydrogen gas produced/incident
light energy) of 6 to 8% using Rhodobacter sp. in laboratory experiments
Thank you
References: Online notes, notes from research papers
and Books by google search Engine