Group presentation on HPLC (High-performance liquid chromatography). The group members are listed. HPLC is introduced as a powerful analytical technique that uses high pressure to pump a mobile phase through a column packed with porous particles. The main principles of separation in HPLC are adsorption and differences in affinity between sample components and the stationary phase. The main types of HPLC are normal phase, reverse phase, ion-exchange, size-exclusion, and affinity chromatography. Key components of HPLC instrumentation are the pump, injector, column, detector, and data system. Common detectors are UV-Vis, fluorescence, and mass spectrometry. Applications of HPLC include analysis in pharmaceuticals, forensics, clinical settings, and more